Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes
This study is a continuation of our previous work [Murugan, S.S., Balakrishnamurthy, P., Mathew, Y.J., 2007. Antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Phytother. Res. 21, 545–547], in search of possible antimutagenic properties in broccoli flower hea...
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| Veröffentlicht in: | Food and chemical toxicology 2008-10, Vol.46 (10), p.3351-3353 |
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| creator | Anupama, M. Murgan, S.S. Balakrishna Murthy, P. |
| description | This study is a continuation of our previous work [Murugan, S.S., Balakrishnamurthy, P., Mathew, Y.J., 2007. Antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Phytother. Res. 21, 545–547], in search of possible antimutagenic properties in broccoli flower head extracts. In the present investigation, the effect of addition of ethanol extract of broccoli flower head on mitomycin-C (MMC) induced sister chromatid exchange (SCE) in cultured human peripheral blood lymphocytes was investigated.
Broccoli flower head was extracted in ethanol using either acetone or ethanol as solvents. The extract was tested at final concentrations of 200 and 400
μg/ml culture and set for SCE assay. MMC at a concentration of 1
μg/ml and the test concentrations of broccoli flower head were added to the culture following 48
h from the initiation of culture. Enumeration of SCE in second division mitotic cells indicated that broccoli flower head extract significantly reduced MMC induced SCEs at both the concentrations tested. This observation is in line with our earlier finding and confirms to the presence of antimutagenic principles in broccoli flower head extract. |
| doi_str_mv | 10.1016/j.fct.2008.08.009 |
| format | Article |
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Broccoli flower head was extracted in ethanol using either acetone or ethanol as solvents. The extract was tested at final concentrations of 200 and 400
μg/ml culture and set for SCE assay. MMC at a concentration of 1
μg/ml and the test concentrations of broccoli flower head were added to the culture following 48
h from the initiation of culture. Enumeration of SCE in second division mitotic cells indicated that broccoli flower head extract significantly reduced MMC induced SCEs at both the concentrations tested. This observation is in line with our earlier finding and confirms to the presence of antimutagenic principles in broccoli flower head extract.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2008.08.009</identifier><identifier>PMID: 18775466</identifier><identifier>CODEN: FCTOD7</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Antigenotoxic ; Antimutagenic ; Biological and medical sciences ; Brassica ; Brassica - chemistry ; Broccoli flower head ; Cells, Cultured ; Chemical mutagenesis ; Dose-Response Relationship, Drug ; Flowers - chemistry ; Human lymphocytes ; Humans ; Lymphocytes - drug effects ; Medical sciences ; Mitomycin - toxicity ; Mitomycin-C ; Mutagenicity Tests ; Mutagens - toxicity ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Salmonella ; Sister chromatid exchange ; Sister Chromatid Exchange - drug effects ; Toxicology</subject><ispartof>Food and chemical toxicology, 2008-10, Vol.46 (10), p.3351-3353</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-db2c4f82a9071d7708a0005894bdf4659c1c7b6b1e6adb4a5f6642b4d884f0c03</citedby><cites>FETCH-LOGICAL-c412t-db2c4f82a9071d7708a0005894bdf4659c1c7b6b1e6adb4a5f6642b4d884f0c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fct.2008.08.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20804684$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18775466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anupama, M.</creatorcontrib><creatorcontrib>Murgan, S.S.</creatorcontrib><creatorcontrib>Balakrishna Murthy, P.</creatorcontrib><title>Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>This study is a continuation of our previous work [Murugan, S.S., Balakrishnamurthy, P., Mathew, Y.J., 2007. Antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Phytother. Res. 21, 545–547], in search of possible antimutagenic properties in broccoli flower head extracts. In the present investigation, the effect of addition of ethanol extract of broccoli flower head on mitomycin-C (MMC) induced sister chromatid exchange (SCE) in cultured human peripheral blood lymphocytes was investigated.
Broccoli flower head was extracted in ethanol using either acetone or ethanol as solvents. The extract was tested at final concentrations of 200 and 400
μg/ml culture and set for SCE assay. MMC at a concentration of 1
μg/ml and the test concentrations of broccoli flower head were added to the culture following 48
h from the initiation of culture. Enumeration of SCE in second division mitotic cells indicated that broccoli flower head extract significantly reduced MMC induced SCEs at both the concentrations tested. This observation is in line with our earlier finding and confirms to the presence of antimutagenic principles in broccoli flower head extract.</description><subject>Antigenotoxic</subject><subject>Antimutagenic</subject><subject>Biological and medical sciences</subject><subject>Brassica</subject><subject>Brassica - chemistry</subject><subject>Broccoli flower head</subject><subject>Cells, Cultured</subject><subject>Chemical mutagenesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flowers - chemistry</subject><subject>Human lymphocytes</subject><subject>Humans</subject><subject>Lymphocytes - drug effects</subject><subject>Medical sciences</subject><subject>Mitomycin - toxicity</subject><subject>Mitomycin-C</subject><subject>Mutagenicity Tests</subject><subject>Mutagens - toxicity</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Salmonella</subject><subject>Sister chromatid exchange</subject><subject>Sister Chromatid Exchange - drug effects</subject><subject>Toxicology</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEGL1DAUx4Mo7rj6AbxILu6tY9JJkxRPOqzrwoIXPYf05dVmaJsxSV3n25syg94WHjwIv__Lnx8hbznbcsblh8O2h7ytGdPbdVj7jGy4VrtK7hr-nGxYrXQlW95ckVcpHRhjiiv5klwVSDVCyg05fo4BIIye9mN4xEgHtI7inxwtZBrRLYCJTj6H6QR-rvbUz-ubo8mnXHgYYphs9msIBjv_xEJQWMa8lDQdlsnOdDxNxyHAKWN6TV70dkz45rKvyY8vt9_3X6uHb3f3-08PFQhe58p1NYhe17YtnZ1STNtSv9Gt6FwvZNMCB9XJjqO0rhO26aUUdSec1qJnwHbX5OZ89xjDrwVTNpNPgONoZwxLMjXjTEktC8jPIMSQUsTeHKOfbDwZzsyq2RxM0WxWzWYd1pbMu8vxpZvQ_U9cvBbg_QWwCezYRzuDT_-4mmkmpBaF-3jmsKj47TGaBB7n4tdHLJ-64J-o8RdrmZyl</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Anupama, M.</creator><creator>Murgan, S.S.</creator><creator>Balakrishna Murthy, P.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20081001</creationdate><title>Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes</title><author>Anupama, M. ; Murgan, S.S. ; Balakrishna Murthy, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-db2c4f82a9071d7708a0005894bdf4659c1c7b6b1e6adb4a5f6642b4d884f0c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antigenotoxic</topic><topic>Antimutagenic</topic><topic>Biological and medical sciences</topic><topic>Brassica</topic><topic>Brassica - chemistry</topic><topic>Broccoli flower head</topic><topic>Cells, Cultured</topic><topic>Chemical mutagenesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Flowers - chemistry</topic><topic>Human lymphocytes</topic><topic>Humans</topic><topic>Lymphocytes - drug effects</topic><topic>Medical sciences</topic><topic>Mitomycin - toxicity</topic><topic>Mitomycin-C</topic><topic>Mutagenicity Tests</topic><topic>Mutagens - toxicity</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Salmonella</topic><topic>Sister chromatid exchange</topic><topic>Sister Chromatid Exchange - drug effects</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anupama, M.</creatorcontrib><creatorcontrib>Murgan, S.S.</creatorcontrib><creatorcontrib>Balakrishna Murthy, P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anupama, M.</au><au>Murgan, S.S.</au><au>Balakrishna Murthy, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>46</volume><issue>10</issue><spage>3351</spage><epage>3353</epage><pages>3351-3353</pages><issn>0278-6915</issn><eissn>1873-6351</eissn><coden>FCTOD7</coden><abstract>This study is a continuation of our previous work [Murugan, S.S., Balakrishnamurthy, P., Mathew, Y.J., 2007. Antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Phytother. Res. 21, 545–547], in search of possible antimutagenic properties in broccoli flower head extracts. In the present investigation, the effect of addition of ethanol extract of broccoli flower head on mitomycin-C (MMC) induced sister chromatid exchange (SCE) in cultured human peripheral blood lymphocytes was investigated.
Broccoli flower head was extracted in ethanol using either acetone or ethanol as solvents. The extract was tested at final concentrations of 200 and 400
μg/ml culture and set for SCE assay. MMC at a concentration of 1
μg/ml and the test concentrations of broccoli flower head were added to the culture following 48
h from the initiation of culture. Enumeration of SCE in second division mitotic cells indicated that broccoli flower head extract significantly reduced MMC induced SCEs at both the concentrations tested. This observation is in line with our earlier finding and confirms to the presence of antimutagenic principles in broccoli flower head extract.</abstract><cop>Oxford</cop><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>18775466</pmid><doi>10.1016/j.fct.2008.08.009</doi><tpages>3</tpages></addata></record> |
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| subjects | Antigenotoxic Antimutagenic Biological and medical sciences Brassica Brassica - chemistry Broccoli flower head Cells, Cultured Chemical mutagenesis Dose-Response Relationship, Drug Flowers - chemistry Human lymphocytes Humans Lymphocytes - drug effects Medical sciences Mitomycin - toxicity Mitomycin-C Mutagenicity Tests Mutagens - toxicity Plant Extracts - chemistry Plant Extracts - pharmacology Salmonella Sister chromatid exchange Sister Chromatid Exchange - drug effects Toxicology |
| title | Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes |
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