Quenching autofluorescence of insect tissues for in situ detection of endosymbionts

Oligonucleotide-probed fluorescent in situ hybridization (FISH) targeting 16S rRNA is a powerful technique for detecting and characterizing bacterial cells in environmental samples without cultivation; however, general application of the technique to insect endosymbionts has been hindered by the str...

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Veröffentlicht in:	Applied Entomology and Zoology 2009/05/25, Vol.44(2), pp.281-291
Hauptverfasser:	Koga, R.(National Inst. of Advanced Industrial Science and Technology Tsukuba, Ibaraki (Japan)), Tsuchida, T, Fukatsu, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Aphididae ARN Carnoy's solution CHEMILUMINESCENCE METHOD FISH FLUORESCENCE FLUORESCENCIA Fluorescent in situ hybridization HIBRIDACION HYBRIDATION HYBRIDIZATION HYDROGEN PEROXIDE METHODE PAR CHIMILUMINESCENCE METODO DE QUIMIOLUMINESCENCIA PEROXIDO DE HIDROGENO PEROXYDE D'HYDROGENE PESCADO POISSON (ALIMENT) quenching autofluorescence of insect tissues RNA SIMBIONTICO SYMBIONTS SYMBIOTE
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