Evaluation of the Sensitivity of the Flow Through Assay for detection of White Spot Syndrome Virus (WSSV) using a cocktail of monoclonal antibodies
A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as a...
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| Veröffentlicht in: | Journal of immunological methods 2018-05, Vol.456, p.54-60 |
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| container_title | Journal of immunological methods |
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| creator | Inchara, U.B. Sathish, R.P. Shankar, K.M. Abhiman, P.B. Prakash, P. |
| description | A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as antigen for evaluation. Out of the total 11 cocktails and four individual of MAbs, 2 MAb cocktails C-05 + C-56 and C-14 + C-56 exhibited highest sensitivity in the FTA. The two MAb cocktail were 100 times more sensitive than 1-step PCR and nearly equivalent to 2-step PCR for the detection of WSSV. The detection limit of WSSV by MAb cocktail increased by two fold compared to the single MAb C-05 currently being used in (FTA).
•The sensitivity of detection of WSSV by FTA using a panel of MAb and their cocktail was evaluated.•The detection limit of WSSV by MAbs cocktail was found to increase by two fold relative to single MAb C-05.•The MAb cocktail was 100 times more sensitive than 1st step PCR and nearly equivalent to 2nd step PCR. |
| doi_str_mv | 10.1016/j.jim.2018.02.012 |
| format | Article |
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•The sensitivity of detection of WSSV by FTA using a panel of MAb and their cocktail was evaluated.•The detection limit of WSSV by MAbs cocktail was found to increase by two fold relative to single MAb C-05.•The MAb cocktail was 100 times more sensitive than 1st step PCR and nearly equivalent to 2nd step PCR.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2018.02.012</identifier><identifier>PMID: 29486144</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antibodies, Monoclonal - genetics ; Antibodies, Monoclonal - immunology ; Cocktail ; FTA ; Immunodetection ; Monoclonal antibodies ; Polymerase Chain Reaction ; White spot syndrome virus 1 - genetics ; White spot syndrome virus 1 - immunology ; White spot syndrome virus 1 - isolation & purification ; WSSV</subject><ispartof>Journal of immunological methods, 2018-05, Vol.456, p.54-60</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-352035e3f2700f053e044db5fd11497cd9527c115729e7aa96272e6809f271bc3</citedby><cites>FETCH-LOGICAL-c353t-352035e3f2700f053e044db5fd11497cd9527c115729e7aa96272e6809f271bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022175917305161$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29486144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inchara, U.B.</creatorcontrib><creatorcontrib>Sathish, R.P.</creatorcontrib><creatorcontrib>Shankar, K.M.</creatorcontrib><creatorcontrib>Abhiman, P.B.</creatorcontrib><creatorcontrib>Prakash, P.</creatorcontrib><title>Evaluation of the Sensitivity of the Flow Through Assay for detection of White Spot Syndrome Virus (WSSV) using a cocktail of monoclonal antibodies</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as antigen for evaluation. Out of the total 11 cocktails and four individual of MAbs, 2 MAb cocktails C-05 + C-56 and C-14 + C-56 exhibited highest sensitivity in the FTA. The two MAb cocktail were 100 times more sensitive than 1-step PCR and nearly equivalent to 2-step PCR for the detection of WSSV. The detection limit of WSSV by MAb cocktail increased by two fold compared to the single MAb C-05 currently being used in (FTA).
•The sensitivity of detection of WSSV by FTA using a panel of MAb and their cocktail was evaluated.•The detection limit of WSSV by MAbs cocktail was found to increase by two fold relative to single MAb C-05.•The MAb cocktail was 100 times more sensitive than 1st step PCR and nearly equivalent to 2nd step PCR.</description><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Cocktail</subject><subject>FTA</subject><subject>Immunodetection</subject><subject>Monoclonal antibodies</subject><subject>Polymerase Chain Reaction</subject><subject>White spot syndrome virus 1 - genetics</subject><subject>White spot syndrome virus 1 - immunology</subject><subject>White spot syndrome virus 1 - isolation & purification</subject><subject>WSSV</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu2zAQRYmiReM8PqCbgst0IWVIiaKJroIgSQsEyMJpsiRoahTTlUSXpFz4O_rDpeG4y64GGNxzgZlDyCcGJQPWXK3LtRtKDmxeAi-B8XdkxuaSF1KBeE9mAJwXTAp1Qk5jXAMAgwY-khOu6nnD6npG_txuTT-Z5PxIfUfTCukCx-iS27q0O67uev-bPq2Cn15X9DpGs6OdD7TFhPaIvqxcyvDGJ7rYjW3wA9JnF6ZIL18Wi-cvdIpufKWGWm9_JuP6PTT40dvej6anZkxu6VuH8Zx86Ewf8eJtnpEfd7dPN9-Kh8f77zfXD4WtRJWKSnCoBFYdlwAdiAqhrtul6FrGaiVtqwSXljEhuUJpjGq45NjMQWWCLW11Ri4PvZvgf00Ykx5ctNj3ZkQ_Rc0BFGdCCJmj7BC1wccYsNOb4AYTdpqB3rvQa51d6L0LDVxnF5n5_FY_LQds_xHH5-fA10MA85Fbh0FH63C02LqQ_6pb7_5T_xfQFJoy</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Inchara, U.B.</creator><creator>Sathish, R.P.</creator><creator>Shankar, K.M.</creator><creator>Abhiman, P.B.</creator><creator>Prakash, P.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201805</creationdate><title>Evaluation of the Sensitivity of the Flow Through Assay for detection of White Spot Syndrome Virus (WSSV) using a cocktail of monoclonal antibodies</title><author>Inchara, U.B. ; Sathish, R.P. ; Shankar, K.M. ; Abhiman, P.B. ; Prakash, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-352035e3f2700f053e044db5fd11497cd9527c115729e7aa96272e6809f271bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Cocktail</topic><topic>FTA</topic><topic>Immunodetection</topic><topic>Monoclonal antibodies</topic><topic>Polymerase Chain Reaction</topic><topic>White spot syndrome virus 1 - genetics</topic><topic>White spot syndrome virus 1 - immunology</topic><topic>White spot syndrome virus 1 - isolation & purification</topic><topic>WSSV</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inchara, U.B.</creatorcontrib><creatorcontrib>Sathish, R.P.</creatorcontrib><creatorcontrib>Shankar, K.M.</creatorcontrib><creatorcontrib>Abhiman, P.B.</creatorcontrib><creatorcontrib>Prakash, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inchara, U.B.</au><au>Sathish, R.P.</au><au>Shankar, K.M.</au><au>Abhiman, P.B.</au><au>Prakash, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the Sensitivity of the Flow Through Assay for detection of White Spot Syndrome Virus (WSSV) using a cocktail of monoclonal antibodies</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>2018-05</date><risdate>2018</risdate><volume>456</volume><spage>54</spage><epage>60</epage><pages>54-60</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as antigen for evaluation. Out of the total 11 cocktails and four individual of MAbs, 2 MAb cocktails C-05 + C-56 and C-14 + C-56 exhibited highest sensitivity in the FTA. The two MAb cocktail were 100 times more sensitive than 1-step PCR and nearly equivalent to 2-step PCR for the detection of WSSV. The detection limit of WSSV by MAb cocktail increased by two fold compared to the single MAb C-05 currently being used in (FTA).
•The sensitivity of detection of WSSV by FTA using a panel of MAb and their cocktail was evaluated.•The detection limit of WSSV by MAbs cocktail was found to increase by two fold relative to single MAb C-05.•The MAb cocktail was 100 times more sensitive than 1st step PCR and nearly equivalent to 2nd step PCR.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29486144</pmid><doi>10.1016/j.jim.2018.02.012</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 2018-05, Vol.456, p.54-60 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Cocktail FTA Immunodetection Monoclonal antibodies Polymerase Chain Reaction White spot syndrome virus 1 - genetics White spot syndrome virus 1 - immunology White spot syndrome virus 1 - isolation & purification WSSV |
| title | Evaluation of the Sensitivity of the Flow Through Assay for detection of White Spot Syndrome Virus (WSSV) using a cocktail of monoclonal antibodies |
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