Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD45RB+ dendritic cells
Background Bone marrow‐derived mesenchymal stem cells (BMSCs) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSCs on immune tolerance of allogeneic heart transplantation and the involve...
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| Veröffentlicht in: | Clinical transplantation 2018-04, Vol.32 (4), p.e13231-n/a |
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| container_title | Clinical transplantation |
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| creator | Hua, Fei Chen, Yueqiu Yang, Ziying Teng, Xiaomei Huang, Haoyue Shen, Zhenya |
| description | Background
Bone marrow‐derived mesenchymal stem cells (BMSCs) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSCs on immune tolerance of allogeneic heart transplantation and the involvement of CD45RB+ dendritic cells (DCs).
Methods
Bone marrow‐derived DCs and BMSCs were co‐cultured, with CD45RB expression on the surface of DCs measured by flow cytometry. qRT‐PCR and Western blotting were used to detect mRNA and protein levels. Cytometric bead array was performed to determine the serum level of IL‐10. Survival time of transplanted heart and expression of CD4+, CD8+, IL‐2, IL‐4, IL‐10, IFN‐γ were determined. Immunofluorescence assay was employed to determine intensity of C3d and C4d.
Results
DCs co‐cultured with BMSCs showed increased CD45RB and Foxp3 levels. CD45RB+ DCs co‐cultured with T‐cells CD4+ displayed increased T‐cell CD4+ Foxp3 ratio and IL‐10 than DCs. Both of them extended survival time of transplanted heart, decreased histopathological classification and score, intensity of C3d, C4d, proportion of CD4+, expression levels of IL‐2 and IFN‐γ, and increased the CD4+ Foxp3 ratio and levels of IL‐4 and IL‐10. CD45RB+ DCs achieved better protective effects than DCs.
Conclusion
BMSCs increased the expression of CD45RB in the bone marrow‐derived DCs, thereby strengthening immunosuppression capacity of T cells and immune tolerance of allogeneic heart transplantation. |
| doi_str_mv | 10.1111/ctr.13231 |
| format | Article |
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Bone marrow‐derived mesenchymal stem cells (BMSCs) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSCs on immune tolerance of allogeneic heart transplantation and the involvement of CD45RB+ dendritic cells (DCs).
Methods
Bone marrow‐derived DCs and BMSCs were co‐cultured, with CD45RB expression on the surface of DCs measured by flow cytometry. qRT‐PCR and Western blotting were used to detect mRNA and protein levels. Cytometric bead array was performed to determine the serum level of IL‐10. Survival time of transplanted heart and expression of CD4+, CD8+, IL‐2, IL‐4, IL‐10, IFN‐γ were determined. Immunofluorescence assay was employed to determine intensity of C3d and C4d.
Results
DCs co‐cultured with BMSCs showed increased CD45RB and Foxp3 levels. CD45RB+ DCs co‐cultured with T‐cells CD4+ displayed increased T‐cell CD4+ Foxp3 ratio and IL‐10 than DCs. Both of them extended survival time of transplanted heart, decreased histopathological classification and score, intensity of C3d, C4d, proportion of CD4+, expression levels of IL‐2 and IFN‐γ, and increased the CD4+ Foxp3 ratio and levels of IL‐4 and IL‐10. CD45RB+ DCs achieved better protective effects than DCs.
Conclusion
BMSCs increased the expression of CD45RB in the bone marrow‐derived DCs, thereby strengthening immunosuppression capacity of T cells and immune tolerance of allogeneic heart transplantation.</description><identifier>ISSN: 0902-0063</identifier><identifier>EISSN: 1399-0012</identifier><identifier>DOI: 10.1111/ctr.13231</identifier><identifier>PMID: 29488658</identifier><language>eng</language><publisher>Denmark</publisher><subject>allogeneic heart transplantation ; bone marrow mesenchymal stem cell ; CD4 ; CD8 ; dendritic cell ; immune tolerance</subject><ispartof>Clinical transplantation, 2018-04, Vol.32 (4), p.e13231-n/a</ispartof><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-8221-9628</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fctr.13231$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fctr.13231$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29488658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hua, Fei</creatorcontrib><creatorcontrib>Chen, Yueqiu</creatorcontrib><creatorcontrib>Yang, Ziying</creatorcontrib><creatorcontrib>Teng, Xiaomei</creatorcontrib><creatorcontrib>Huang, Haoyue</creatorcontrib><creatorcontrib>Shen, Zhenya</creatorcontrib><title>Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD45RB+ dendritic cells</title><title>Clinical transplantation</title><addtitle>Clin Transplant</addtitle><description>Background
Bone marrow‐derived mesenchymal stem cells (BMSCs) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSCs on immune tolerance of allogeneic heart transplantation and the involvement of CD45RB+ dendritic cells (DCs).
Methods
Bone marrow‐derived DCs and BMSCs were co‐cultured, with CD45RB expression on the surface of DCs measured by flow cytometry. qRT‐PCR and Western blotting were used to detect mRNA and protein levels. Cytometric bead array was performed to determine the serum level of IL‐10. Survival time of transplanted heart and expression of CD4+, CD8+, IL‐2, IL‐4, IL‐10, IFN‐γ were determined. Immunofluorescence assay was employed to determine intensity of C3d and C4d.
Results
DCs co‐cultured with BMSCs showed increased CD45RB and Foxp3 levels. CD45RB+ DCs co‐cultured with T‐cells CD4+ displayed increased T‐cell CD4+ Foxp3 ratio and IL‐10 than DCs. Both of them extended survival time of transplanted heart, decreased histopathological classification and score, intensity of C3d, C4d, proportion of CD4+, expression levels of IL‐2 and IFN‐γ, and increased the CD4+ Foxp3 ratio and levels of IL‐4 and IL‐10. CD45RB+ DCs achieved better protective effects than DCs.
Conclusion
BMSCs increased the expression of CD45RB in the bone marrow‐derived DCs, thereby strengthening immunosuppression capacity of T cells and immune tolerance of allogeneic heart transplantation.</description><subject>allogeneic heart transplantation</subject><subject>bone marrow mesenchymal stem cell</subject><subject>CD4</subject><subject>CD8</subject><subject>dendritic cell</subject><subject>immune tolerance</subject><issn>0902-0063</issn><issn>1399-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNo9kdtOAyEQhonRaK1e-AKGSxPTlsOeuNR6TJpomnq9YZfZuoaFCqxNH8T3ldYqNzPDfPND5kfogpIxjWdSBzemnHF6gAaUCzEihLJDNCCCsJhn_ASdev8RbzOapcfohImkKLK0GKDvV2cD1KH9AixjsAbbBlfWAO6kc3aNO_Bg6vdNJzX2ATpcg9Yetwa3XddHLlgNTpoatpNSa7sEA22N30G6gENs-ZWWJsiderXBDpa9jpVZ4uldks5vr7ECo1wb4tRO_QwdNVJ7ON_HIXp7uF9Mn0azl8fn6c1stGIppaNUqaahWcEU55DTSikBlHOimkpkaSMLkiey4iwXOdC6EKzJiyQReU1lrkDkfIiufnVXzn724EPZtX77A2nA9r5khAhG41Nb9HKP9lUHqly5Ni5oU_6tMgKTX2Ddatj89ykptx6V0aNy51E5Xcx3Cf8B3Q-GIQ</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Hua, Fei</creator><creator>Chen, Yueqiu</creator><creator>Yang, Ziying</creator><creator>Teng, Xiaomei</creator><creator>Huang, Haoyue</creator><creator>Shen, Zhenya</creator><scope>NPM</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8221-9628</orcidid></search><sort><creationdate>201804</creationdate><title>Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD45RB+ dendritic cells</title><author>Hua, Fei ; Chen, Yueqiu ; Yang, Ziying ; Teng, Xiaomei ; Huang, Haoyue ; Shen, Zhenya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2511-5ddff1682d33e71bdd9e1330dfb965fa8074ab32797e1c892f784497c1a7de973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>allogeneic heart transplantation</topic><topic>bone marrow mesenchymal stem cell</topic><topic>CD4</topic><topic>CD8</topic><topic>dendritic cell</topic><topic>immune tolerance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hua, Fei</creatorcontrib><creatorcontrib>Chen, Yueqiu</creatorcontrib><creatorcontrib>Yang, Ziying</creatorcontrib><creatorcontrib>Teng, Xiaomei</creatorcontrib><creatorcontrib>Huang, Haoyue</creatorcontrib><creatorcontrib>Shen, Zhenya</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hua, Fei</au><au>Chen, Yueqiu</au><au>Yang, Ziying</au><au>Teng, Xiaomei</au><au>Huang, Haoyue</au><au>Shen, Zhenya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD45RB+ dendritic cells</atitle><jtitle>Clinical transplantation</jtitle><addtitle>Clin Transplant</addtitle><date>2018-04</date><risdate>2018</risdate><volume>32</volume><issue>4</issue><spage>e13231</spage><epage>n/a</epage><pages>e13231-n/a</pages><issn>0902-0063</issn><eissn>1399-0012</eissn><abstract>Background
Bone marrow‐derived mesenchymal stem cells (BMSCs) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSCs on immune tolerance of allogeneic heart transplantation and the involvement of CD45RB+ dendritic cells (DCs).
Methods
Bone marrow‐derived DCs and BMSCs were co‐cultured, with CD45RB expression on the surface of DCs measured by flow cytometry. qRT‐PCR and Western blotting were used to detect mRNA and protein levels. Cytometric bead array was performed to determine the serum level of IL‐10. Survival time of transplanted heart and expression of CD4+, CD8+, IL‐2, IL‐4, IL‐10, IFN‐γ were determined. Immunofluorescence assay was employed to determine intensity of C3d and C4d.
Results
DCs co‐cultured with BMSCs showed increased CD45RB and Foxp3 levels. CD45RB+ DCs co‐cultured with T‐cells CD4+ displayed increased T‐cell CD4+ Foxp3 ratio and IL‐10 than DCs. Both of them extended survival time of transplanted heart, decreased histopathological classification and score, intensity of C3d, C4d, proportion of CD4+, expression levels of IL‐2 and IFN‐γ, and increased the CD4+ Foxp3 ratio and levels of IL‐4 and IL‐10. CD45RB+ DCs achieved better protective effects than DCs.
Conclusion
BMSCs increased the expression of CD45RB in the bone marrow‐derived DCs, thereby strengthening immunosuppression capacity of T cells and immune tolerance of allogeneic heart transplantation.</abstract><cop>Denmark</cop><pmid>29488658</pmid><doi>10.1111/ctr.13231</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8221-9628</orcidid></addata></record> |
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| language | eng |
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| source | Wiley Online Library (Online service) |
| subjects | allogeneic heart transplantation bone marrow mesenchymal stem cell CD4 CD8 dendritic cell immune tolerance |
| title | Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD45RB+ dendritic cells |
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