Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells
Purpose: Gastric cancer is one of the most frequently diagnosed malignancies in the world, especially in Korea and Japan. To understand the molecular mechanism associated with gastric carcinogenesis, we attempted to identify novel gastric cancer–related genes using a novel 2K cDNA microarray. Experi...
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| Veröffentlicht in: | Clinical cancer research 2005-01, Vol.11 (2), p.473-482 |
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| creator | KIM, Jeong-Min SOHN, Ho-Yong KIM, Jong-Guk KIM, Nam-Soon SUN YOUNG YOON OH, Jung-Hwa JIN OK YANG JOO HEON KIM KYU SANG SONG RHO, Seung-Moo HYAN SOOK YOO YONG SUNG KIM |
| description | Purpose: Gastric cancer is one of the most frequently diagnosed malignancies in the world, especially in Korea and Japan. To understand
the molecular mechanism associated with gastric carcinogenesis, we attempted to identify novel gastric cancer–related genes
using a novel 2K cDNA microarray.
Experimental Design: A 2K cDNA microarray was fabricated from 1,995 novel expressed sequence tags (ESTs) showing no hits or a low homology with
ESTs in public databases from our 143,452 ESTs collected from gastric cancer cell lines and tissues. An analysis of the gene
expression for human gastric cancer cell lines to a normal cell line was done using this cDNA microarray. Data for the different
expressed genes were verified using semiquantitative reverse transcription-PCR, Western blotting, and immunohistochemical
staining in the gastric cell lines and tissues.
Results: Forty genes were identified as either up-regulated or down-regulated genes in human gastric cancer cells. Among these, genes
such as SKB1 , NT5C3 , ZNF9 , p30 , CDC20 , and FEN1 , were confirmed to be up-regulated genes in nine gastric cell lines and in 25 pairs of tissue samples from patients by semiquantitative
reverse transcription-PCR. On the other hand, genes such as MT2A and CXX1 were identified as down-regulated genes. In particular, the SKB1 , CDC20 , and FEN1 genes were overexpressed in ≥68% of tissues and the MT2A gene was down-expressed in 72% of the tissues. Western blotting and immunohistochemical analyses for CDC20 and SKB1 showed
overexpression and localization changes of the corresponding protein in human gastric cancer tissues.
Conclusions: Novel genes that are related to human gastric cancer were identified using cDNA microarray developed in our laboratory. In
particular, CDC20 and MT2A represent a potential biomarker of human gastric cancer. These newly identified genes should provide a valuable resource
for understanding the molecular mechanism associated with tumorigenesis of gastric carcinogenesis and for the discovery of
potential diagnostic markers of gastric cancer. |
| doi_str_mv | 10.1158/1078-0432.473.11.2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_20089897</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20089897</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-ec1dfe5e1227637bac60b1f24d43f2dbd0b56e62f08105ca4731960ab27867333</originalsourceid><addsrcrecordid>eNpdkctu1DAUhi0EoqXtC7BA3gCrDL7EsWdZpWWoVIrUy9o6cY5njDLJYGeA7niHviFPgtMZVMTK1vF3zm9_JuQ1ZzPOlfnAmTYFK6WYlVrm0kw8I4dcKV1IUannef8XOCCvUvrKGC85K1-SA64040ayQ_Jw0WI_Bh8cjGHo6eDpAtIYg6M19A7j718P19jBiC1dYI-J3qXQLylQd3Z1Sj8HFweIEe5pPfQjhH46vBq-Y0fPf24ippQbb_DbFvMwegvL9E899P-F0Rq7Lh2TFx66hCf79YjcfTy_rT8Vl18WF_XpZeEUK8cCHW89KuRC6ErqBlzFGu5F2ZbSi7ZpWaMqrIRnhjPlIEvi84pBI7SptJTyiLzbzd3EIV8wjXYdkss3gB6HbbKCMTM3c51BsQPzY1OK6O0mhjXEe8uZnb7CTqbtZNrmlFyyIje92U_fNmtsn1r27jPwdg9ActD5mBWE9MRVpTRzYTL3fsetwnL1I0S07lFWlogQ3eoxbsqVfwDS16CV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20089897</pqid></control><display><type>article</type><title>Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>KIM, Jeong-Min ; SOHN, Ho-Yong ; KIM, Jong-Guk ; KIM, Nam-Soon ; SUN YOUNG YOON ; OH, Jung-Hwa ; JIN OK YANG ; JOO HEON KIM ; KYU SANG SONG ; RHO, Seung-Moo ; HYAN SOOK YOO ; YONG SUNG KIM</creator><creatorcontrib>KIM, Jeong-Min ; SOHN, Ho-Yong ; KIM, Jong-Guk ; KIM, Nam-Soon ; SUN YOUNG YOON ; OH, Jung-Hwa ; JIN OK YANG ; JOO HEON KIM ; KYU SANG SONG ; RHO, Seung-Moo ; HYAN SOOK YOO ; YONG SUNG KIM</creatorcontrib><description>Purpose: Gastric cancer is one of the most frequently diagnosed malignancies in the world, especially in Korea and Japan. To understand
the molecular mechanism associated with gastric carcinogenesis, we attempted to identify novel gastric cancer–related genes
using a novel 2K cDNA microarray.
Experimental Design: A 2K cDNA microarray was fabricated from 1,995 novel expressed sequence tags (ESTs) showing no hits or a low homology with
ESTs in public databases from our 143,452 ESTs collected from gastric cancer cell lines and tissues. An analysis of the gene
expression for human gastric cancer cell lines to a normal cell line was done using this cDNA microarray. Data for the different
expressed genes were verified using semiquantitative reverse transcription-PCR, Western blotting, and immunohistochemical
staining in the gastric cell lines and tissues.
Results: Forty genes were identified as either up-regulated or down-regulated genes in human gastric cancer cells. Among these, genes
such as SKB1 , NT5C3 , ZNF9 , p30 , CDC20 , and FEN1 , were confirmed to be up-regulated genes in nine gastric cell lines and in 25 pairs of tissue samples from patients by semiquantitative
reverse transcription-PCR. On the other hand, genes such as MT2A and CXX1 were identified as down-regulated genes. In particular, the SKB1 , CDC20 , and FEN1 genes were overexpressed in ≥68% of tissues and the MT2A gene was down-expressed in 72% of the tissues. Western blotting and immunohistochemical analyses for CDC20 and SKB1 showed
overexpression and localization changes of the corresponding protein in human gastric cancer tissues.
Conclusions: Novel genes that are related to human gastric cancer were identified using cDNA microarray developed in our laboratory. In
particular, CDC20 and MT2A represent a potential biomarker of human gastric cancer. These newly identified genes should provide a valuable resource
for understanding the molecular mechanism associated with tumorigenesis of gastric carcinogenesis and for the discovery of
potential diagnostic markers of gastric cancer.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.473.11.2</identifier><identifier>PMID: 15701830</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; biomarker ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Blotting, Western ; carcinogenesis ; Cdc20 Proteins ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; Expressed Sequence Tags ; expression profiling ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Immunoenzyme Techniques ; Medical sciences ; Oligonucleotide Array Sequence Analysis ; Pharmacology. Drug treatments ; Protein Methyltransferases - genetics ; Protein Methyltransferases - metabolism ; Protein-Arginine N-Methyltransferases ; Reverse Transcriptase Polymerase Chain Reaction ; semiquantitative RT-PCR ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Tumor Cells, Cultured</subject><ispartof>Clinical cancer research, 2005-01, Vol.11 (2), p.473-482</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-ec1dfe5e1227637bac60b1f24d43f2dbd0b56e62f08105ca4731960ab27867333</citedby><cites>FETCH-LOGICAL-c504t-ec1dfe5e1227637bac60b1f24d43f2dbd0b56e62f08105ca4731960ab27867333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16438928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15701830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KIM, Jeong-Min</creatorcontrib><creatorcontrib>SOHN, Ho-Yong</creatorcontrib><creatorcontrib>KIM, Jong-Guk</creatorcontrib><creatorcontrib>KIM, Nam-Soon</creatorcontrib><creatorcontrib>SUN YOUNG YOON</creatorcontrib><creatorcontrib>OH, Jung-Hwa</creatorcontrib><creatorcontrib>JIN OK YANG</creatorcontrib><creatorcontrib>JOO HEON KIM</creatorcontrib><creatorcontrib>KYU SANG SONG</creatorcontrib><creatorcontrib>RHO, Seung-Moo</creatorcontrib><creatorcontrib>HYAN SOOK YOO</creatorcontrib><creatorcontrib>YONG SUNG KIM</creatorcontrib><title>Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: Gastric cancer is one of the most frequently diagnosed malignancies in the world, especially in Korea and Japan. To understand
the molecular mechanism associated with gastric carcinogenesis, we attempted to identify novel gastric cancer–related genes
using a novel 2K cDNA microarray.
Experimental Design: A 2K cDNA microarray was fabricated from 1,995 novel expressed sequence tags (ESTs) showing no hits or a low homology with
ESTs in public databases from our 143,452 ESTs collected from gastric cancer cell lines and tissues. An analysis of the gene
expression for human gastric cancer cell lines to a normal cell line was done using this cDNA microarray. Data for the different
expressed genes were verified using semiquantitative reverse transcription-PCR, Western blotting, and immunohistochemical
staining in the gastric cell lines and tissues.
Results: Forty genes were identified as either up-regulated or down-regulated genes in human gastric cancer cells. Among these, genes
such as SKB1 , NT5C3 , ZNF9 , p30 , CDC20 , and FEN1 , were confirmed to be up-regulated genes in nine gastric cell lines and in 25 pairs of tissue samples from patients by semiquantitative
reverse transcription-PCR. On the other hand, genes such as MT2A and CXX1 were identified as down-regulated genes. In particular, the SKB1 , CDC20 , and FEN1 genes were overexpressed in ≥68% of tissues and the MT2A gene was down-expressed in 72% of the tissues. Western blotting and immunohistochemical analyses for CDC20 and SKB1 showed
overexpression and localization changes of the corresponding protein in human gastric cancer tissues.
Conclusions: Novel genes that are related to human gastric cancer were identified using cDNA microarray developed in our laboratory. In
particular, CDC20 and MT2A represent a potential biomarker of human gastric cancer. These newly identified genes should provide a valuable resource
for understanding the molecular mechanism associated with tumorigenesis of gastric carcinogenesis and for the discovery of
potential diagnostic markers of gastric cancer.</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>biomarker</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>carcinogenesis</subject><subject>Cdc20 Proteins</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Expressed Sequence Tags</subject><subject>expression profiling</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Medical sciences</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Methyltransferases - genetics</subject><subject>Protein Methyltransferases - metabolism</subject><subject>Protein-Arginine N-Methyltransferases</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>semiquantitative RT-PCR</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctu1DAUhi0EoqXtC7BA3gCrDL7EsWdZpWWoVIrUy9o6cY5njDLJYGeA7niHviFPgtMZVMTK1vF3zm9_JuQ1ZzPOlfnAmTYFK6WYlVrm0kw8I4dcKV1IUannef8XOCCvUvrKGC85K1-SA64040ayQ_Jw0WI_Bh8cjGHo6eDpAtIYg6M19A7j718P19jBiC1dYI-J3qXQLylQd3Z1Sj8HFweIEe5pPfQjhH46vBq-Y0fPf24ippQbb_DbFvMwegvL9E899P-F0Rq7Lh2TFx66hCf79YjcfTy_rT8Vl18WF_XpZeEUK8cCHW89KuRC6ErqBlzFGu5F2ZbSi7ZpWaMqrIRnhjPlIEvi84pBI7SptJTyiLzbzd3EIV8wjXYdkss3gB6HbbKCMTM3c51BsQPzY1OK6O0mhjXEe8uZnb7CTqbtZNrmlFyyIje92U_fNmtsn1r27jPwdg9ActD5mBWE9MRVpTRzYTL3fsetwnL1I0S07lFWlogQ3eoxbsqVfwDS16CV</recordid><startdate>20050115</startdate><enddate>20050115</enddate><creator>KIM, Jeong-Min</creator><creator>SOHN, Ho-Yong</creator><creator>KIM, Jong-Guk</creator><creator>KIM, Nam-Soon</creator><creator>SUN YOUNG YOON</creator><creator>OH, Jung-Hwa</creator><creator>JIN OK YANG</creator><creator>JOO HEON KIM</creator><creator>KYU SANG SONG</creator><creator>RHO, Seung-Moo</creator><creator>HYAN SOOK YOO</creator><creator>YONG SUNG KIM</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20050115</creationdate><title>Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells</title><author>KIM, Jeong-Min ; SOHN, Ho-Yong ; KIM, Jong-Guk ; KIM, Nam-Soon ; SUN YOUNG YOON ; OH, Jung-Hwa ; JIN OK YANG ; JOO HEON KIM ; KYU SANG SONG ; RHO, Seung-Moo ; HYAN SOOK YOO ; YONG SUNG KIM</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-ec1dfe5e1227637bac60b1f24d43f2dbd0b56e62f08105ca4731960ab27867333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>biomarker</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>carcinogenesis</topic><topic>Cdc20 Proteins</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Expressed Sequence Tags</topic><topic>expression profiling</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Medical sciences</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Methyltransferases - genetics</topic><topic>Protein Methyltransferases - metabolism</topic><topic>Protein-Arginine N-Methyltransferases</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>semiquantitative RT-PCR</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIM, Jeong-Min</creatorcontrib><creatorcontrib>SOHN, Ho-Yong</creatorcontrib><creatorcontrib>KIM, Jong-Guk</creatorcontrib><creatorcontrib>KIM, Nam-Soon</creatorcontrib><creatorcontrib>SUN YOUNG YOON</creatorcontrib><creatorcontrib>OH, Jung-Hwa</creatorcontrib><creatorcontrib>JIN OK YANG</creatorcontrib><creatorcontrib>JOO HEON KIM</creatorcontrib><creatorcontrib>KYU SANG SONG</creatorcontrib><creatorcontrib>RHO, Seung-Moo</creatorcontrib><creatorcontrib>HYAN SOOK YOO</creatorcontrib><creatorcontrib>YONG SUNG KIM</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KIM, Jeong-Min</au><au>SOHN, Ho-Yong</au><au>KIM, Jong-Guk</au><au>KIM, Nam-Soon</au><au>SUN YOUNG YOON</au><au>OH, Jung-Hwa</au><au>JIN OK YANG</au><au>JOO HEON KIM</au><au>KYU SANG SONG</au><au>RHO, Seung-Moo</au><au>HYAN SOOK YOO</au><au>YONG SUNG KIM</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2005-01-15</date><risdate>2005</risdate><volume>11</volume><issue>2</issue><spage>473</spage><epage>482</epage><pages>473-482</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><abstract>Purpose: Gastric cancer is one of the most frequently diagnosed malignancies in the world, especially in Korea and Japan. To understand
the molecular mechanism associated with gastric carcinogenesis, we attempted to identify novel gastric cancer–related genes
using a novel 2K cDNA microarray.
Experimental Design: A 2K cDNA microarray was fabricated from 1,995 novel expressed sequence tags (ESTs) showing no hits or a low homology with
ESTs in public databases from our 143,452 ESTs collected from gastric cancer cell lines and tissues. An analysis of the gene
expression for human gastric cancer cell lines to a normal cell line was done using this cDNA microarray. Data for the different
expressed genes were verified using semiquantitative reverse transcription-PCR, Western blotting, and immunohistochemical
staining in the gastric cell lines and tissues.
Results: Forty genes were identified as either up-regulated or down-regulated genes in human gastric cancer cells. Among these, genes
such as SKB1 , NT5C3 , ZNF9 , p30 , CDC20 , and FEN1 , were confirmed to be up-regulated genes in nine gastric cell lines and in 25 pairs of tissue samples from patients by semiquantitative
reverse transcription-PCR. On the other hand, genes such as MT2A and CXX1 were identified as down-regulated genes. In particular, the SKB1 , CDC20 , and FEN1 genes were overexpressed in ≥68% of tissues and the MT2A gene was down-expressed in 72% of the tissues. Western blotting and immunohistochemical analyses for CDC20 and SKB1 showed
overexpression and localization changes of the corresponding protein in human gastric cancer tissues.
Conclusions: Novel genes that are related to human gastric cancer were identified using cDNA microarray developed in our laboratory. In
particular, CDC20 and MT2A represent a potential biomarker of human gastric cancer. These newly identified genes should provide a valuable resource
for understanding the molecular mechanism associated with tumorigenesis of gastric carcinogenesis and for the discovery of
potential diagnostic markers of gastric cancer.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>15701830</pmid><doi>10.1158/1078-0432.473.11.2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical cancer research, 2005-01, Vol.11 (2), p.473-482 |
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| source | MEDLINE; American Association for Cancer Research; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antineoplastic agents Biological and medical sciences biomarker Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western carcinogenesis Cdc20 Proteins Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Expressed Sequence Tags expression profiling Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Medical sciences Oligonucleotide Array Sequence Analysis Pharmacology. Drug treatments Protein Methyltransferases - genetics Protein Methyltransferases - metabolism Protein-Arginine N-Methyltransferases Reverse Transcriptase Polymerase Chain Reaction semiquantitative RT-PCR Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Tumor Cells, Cultured |
| title | Identification of Gastric Cancer–Related Genes Using a cDNA Microarray Containing Novel Expressed Sequence Tags Expressed in Gastric Cancer Cells |
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