High-Density Universal 16S rRNA Microarray Analysis Reveals Broader Diversity than Typical Clone Library When Sampling the Environment

Molecular approaches aimed at detection of a broad-range of prokaryotes in the environment routinely rely on classifying heterogeneous 16S rRNA genes amplified by polymerase chain reaction (PCR) using primers with broad specificity. The general method of sampling and categorizing DNA has been to clo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Microbial ecology 2007-04, Vol.53 (3), p.371-383
Hauptverfasser:	DeSantis, Todd Z, Brodie, Eoin L, Moberg, Jordan P, Zubieta, Ingrid X, Piceno, Yvette M, Andersen, Gary L
Format:	Artikel
Sprache:	eng
Schlagworte:	Aerosols Air sampling Arrays Bacteria Bacteria - classification Bacteria - genetics Base Sequence Biodiversity Clones Cloning Cloning, Molecular - methods Community composition Density DNA DNA microarrays DNA Primers - chemistry DNA probes DNA, Bacterial - chemistry Environmental Microbiology Environmental Monitoring - methods Genes Hybridization Libraries Methods Microarray Analysis - methods Microbiology Microorganisms Molecular Sequence Data Nitrospira Nucleotide sequence PCR Planctomycetes Polymerase Chain Reaction Primers Product category rules Prokaryotes RNA, Ribosomal, 16S - genetics rRNA 16S rRNA genes Samples Sampling Sequencing Soil Microbiology Soil samples Soil water Specificity Subsurface water Taxa Water Microbiology Water samples
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!