A member of the Roseobacter clade, Octadecabacter sp., is the dominant symbiont in the brittle star Amphipholis squamata
Abstract Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in many echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. Previous studies on the SCB of A. squamata placed the isolated bacterium, designated as AS1, in the genus Vibri...
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| creator | Morrow, Kathleen M Tedford, Abbey Rose Pankey, M Sabrina Lesser, Michael P |
| description | Abstract
Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in many echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. Previous studies on the SCB of A. squamata placed the isolated bacterium, designated as AS1, in the genus Vibrio (Gammaproteobacteria), but subsequent studies suggested that the SCB of echinoderms belong to the Alphaproteobacteria. This study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic using the 16S rRNA gene and next generation sequencing. Results show the presence of a single dominant bacterial type, within the Roseobacter clade, family Rhodobacteraceae, which composes 70%–80% of the A. squamata microbiome. These Rhodobacteraceae sequences were identified as members of the genus Octadecabacter. Additionally, the original isolate, AS1, from the brittle star A. squamata also belongs in the genus Octadecabacter based on Sanger sequencing of cloned 16S rRNA gene sequences. By comparison, adjacent seawater and sediment porewater communities were significantly more diverse, hosting bacteria in the phyla Proteobacteria, Bacteroidetes, Cyanobacteria, Verrucomicrobia and Actinobacteria. Thus, a distinct SCB community is present in A. squamata that is dominated by a member of the genus Octadecabacter and is identical to the original isolate, AS1, from this brittle star.
Next generation sequencing of 16S rRNA gene reveals identity of dominant symbiont in the brittle star, Amphipholis squamata. |
| doi_str_mv | 10.1093/femsec/fiy030 |
| format | Article |
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Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in many echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. Previous studies on the SCB of A. squamata placed the isolated bacterium, designated as AS1, in the genus Vibrio (Gammaproteobacteria), but subsequent studies suggested that the SCB of echinoderms belong to the Alphaproteobacteria. This study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic using the 16S rRNA gene and next generation sequencing. Results show the presence of a single dominant bacterial type, within the Roseobacter clade, family Rhodobacteraceae, which composes 70%–80% of the A. squamata microbiome. These Rhodobacteraceae sequences were identified as members of the genus Octadecabacter. Additionally, the original isolate, AS1, from the brittle star A. squamata also belongs in the genus Octadecabacter based on Sanger sequencing of cloned 16S rRNA gene sequences. By comparison, adjacent seawater and sediment porewater communities were significantly more diverse, hosting bacteria in the phyla Proteobacteria, Bacteroidetes, Cyanobacteria, Verrucomicrobia and Actinobacteria. Thus, a distinct SCB community is present in A. squamata that is dominated by a member of the genus Octadecabacter and is identical to the original isolate, AS1, from this brittle star.
Next generation sequencing of 16S rRNA gene reveals identity of dominant symbiont in the brittle star, Amphipholis squamata.</description><identifier>ISSN: 1574-6941</identifier><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1093/femsec/fiy030</identifier><identifier>PMID: 29471328</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amphipholis squamata ; Animals ; Bacteria ; Bacteroidetes - genetics ; Bacteroidetes - isolation & purification ; Cyanobacteria ; Cyanobacteria - genetics ; Cyanobacteria - isolation & purification ; DNA, Bacterial - genetics ; Echinodermata ; Ecology ; Endosymbiosis ; Gene sequencing ; Geologic Sediments - microbiology ; Invertebrates ; Microbiological research ; Microbiology ; Microbiomes ; Phylogeny ; Pore water ; Proteobacteria - genetics ; Proteobacteria - isolation & purification ; RNA, Ribosomal, 16S - genetics ; Roseobacter - classification ; Roseobacter - genetics ; Roseobacter - isolation & purification ; rRNA 16S ; Seawater ; Seawater - microbiology ; Sequence Analysis, DNA ; Starfish - microbiology ; Symbiosis ; Waterborne diseases</subject><ispartof>FEMS microbiology ecology, 2018-04, Vol.94 (4), p.1</ispartof><rights>FEMS 2018. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2018</rights><rights>COPYRIGHT 2018 Oxford University Press</rights><rights>Copyright Oxford University Press, UK Apr 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-9a17fb5708b8b4cc7fd4c6e9f1448bb93572a8992219a7cb0739592e1ae76a743</citedby><cites>FETCH-LOGICAL-c526t-9a17fb5708b8b4cc7fd4c6e9f1448bb93572a8992219a7cb0739592e1ae76a743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1598,27901,27902</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/femsec/fiy030$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29471328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morrow, Kathleen M</creatorcontrib><creatorcontrib>Tedford, Abbey Rose</creatorcontrib><creatorcontrib>Pankey, M Sabrina</creatorcontrib><creatorcontrib>Lesser, Michael P</creatorcontrib><title>A member of the Roseobacter clade, Octadecabacter sp., is the dominant symbiont in the brittle star Amphipholis squamata</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in many echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. Previous studies on the SCB of A. squamata placed the isolated bacterium, designated as AS1, in the genus Vibrio (Gammaproteobacteria), but subsequent studies suggested that the SCB of echinoderms belong to the Alphaproteobacteria. This study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic using the 16S rRNA gene and next generation sequencing. Results show the presence of a single dominant bacterial type, within the Roseobacter clade, family Rhodobacteraceae, which composes 70%–80% of the A. squamata microbiome. These Rhodobacteraceae sequences were identified as members of the genus Octadecabacter. Additionally, the original isolate, AS1, from the brittle star A. squamata also belongs in the genus Octadecabacter based on Sanger sequencing of cloned 16S rRNA gene sequences. By comparison, adjacent seawater and sediment porewater communities were significantly more diverse, hosting bacteria in the phyla Proteobacteria, Bacteroidetes, Cyanobacteria, Verrucomicrobia and Actinobacteria. Thus, a distinct SCB community is present in A. squamata that is dominated by a member of the genus Octadecabacter and is identical to the original isolate, AS1, from this brittle star.
Next generation sequencing of 16S rRNA gene reveals identity of dominant symbiont in the brittle star, Amphipholis squamata.</description><subject>Amphipholis squamata</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteroidetes - genetics</subject><subject>Bacteroidetes - isolation & purification</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - isolation & purification</subject><subject>DNA, Bacterial - genetics</subject><subject>Echinodermata</subject><subject>Ecology</subject><subject>Endosymbiosis</subject><subject>Gene sequencing</subject><subject>Geologic Sediments - microbiology</subject><subject>Invertebrates</subject><subject>Microbiological research</subject><subject>Microbiology</subject><subject>Microbiomes</subject><subject>Phylogeny</subject><subject>Pore water</subject><subject>Proteobacteria - genetics</subject><subject>Proteobacteria - isolation & purification</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Roseobacter - classification</subject><subject>Roseobacter - genetics</subject><subject>Roseobacter - isolation & purification</subject><subject>rRNA 16S</subject><subject>Seawater</subject><subject>Seawater - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Starfish - microbiology</subject><subject>Symbiosis</subject><subject>Waterborne diseases</subject><issn>1574-6941</issn><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc1rHSEUxYfS0qRpl90WoZsuMi_qOKMuH6FfEAiUdi3qXPsM4zhRB_L--5q816YtheLiXo6_e7xymuY1wRuCZXfhIGSwF87vcYefNKek56wdJCNPf-tPmhc532BM-o7h580JlYyTjorT5m6LAgQDCUWHyg7Ql5ghGm1LleykRzhH17bUavVRzcvmHPn8QI8x-FnPBeV9MD7Wxs8PFyb5UiZAueiEtmHZ-WUXpzqVb1cddNEvm2dOTxleHetZ8-3D-6-Xn9qr64-fL7dXre3pUFqpCXem51gYYZi13I3MDiAdYUwYI7ueUy2kpJRIza3BvJO9pEA08EFz1p017w6-S4q3K-Sigs8WpknPENesKMZcCtYJXNG3f6E3cU1z3a5SFAtWX6GP1Hc9gfKziyVpe2-qtoPEgxBi6Cu1-QdVzwjB2ziD81X_Y6A9DNgUc07g1JJ80GmvCFb3SatD0uqQdOXfHJddTYDxF_0z2sePx3X5j9cPjO-yHQ</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Morrow, Kathleen M</creator><creator>Tedford, Abbey Rose</creator><creator>Pankey, M Sabrina</creator><creator>Lesser, Michael P</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180401</creationdate><title>A member of the Roseobacter clade, Octadecabacter sp., is the dominant symbiont in the brittle star Amphipholis squamata</title><author>Morrow, Kathleen M ; Tedford, Abbey Rose ; Pankey, M Sabrina ; Lesser, Michael P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-9a17fb5708b8b4cc7fd4c6e9f1448bb93572a8992219a7cb0739592e1ae76a743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amphipholis squamata</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteroidetes - 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Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Morrow, Kathleen M</au><au>Tedford, Abbey Rose</au><au>Pankey, M Sabrina</au><au>Lesser, Michael P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A member of the Roseobacter clade, Octadecabacter sp., is the dominant symbiont in the brittle star Amphipholis squamata</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>94</volume><issue>4</issue><spage>1</spage><pages>1-</pages><issn>1574-6941</issn><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in many echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. Previous studies on the SCB of A. squamata placed the isolated bacterium, designated as AS1, in the genus Vibrio (Gammaproteobacteria), but subsequent studies suggested that the SCB of echinoderms belong to the Alphaproteobacteria. This study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic using the 16S rRNA gene and next generation sequencing. Results show the presence of a single dominant bacterial type, within the Roseobacter clade, family Rhodobacteraceae, which composes 70%–80% of the A. squamata microbiome. These Rhodobacteraceae sequences were identified as members of the genus Octadecabacter. Additionally, the original isolate, AS1, from the brittle star A. squamata also belongs in the genus Octadecabacter based on Sanger sequencing of cloned 16S rRNA gene sequences. By comparison, adjacent seawater and sediment porewater communities were significantly more diverse, hosting bacteria in the phyla Proteobacteria, Bacteroidetes, Cyanobacteria, Verrucomicrobia and Actinobacteria. Thus, a distinct SCB community is present in A. squamata that is dominated by a member of the genus Octadecabacter and is identical to the original isolate, AS1, from this brittle star.
Next generation sequencing of 16S rRNA gene reveals identity of dominant symbiont in the brittle star, Amphipholis squamata.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29471328</pmid><doi>10.1093/femsec/fiy030</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Amphipholis squamata Animals Bacteria Bacteroidetes - genetics Bacteroidetes - isolation & purification Cyanobacteria Cyanobacteria - genetics Cyanobacteria - isolation & purification DNA, Bacterial - genetics Echinodermata Ecology Endosymbiosis Gene sequencing Geologic Sediments - microbiology Invertebrates Microbiological research Microbiology Microbiomes Phylogeny Pore water Proteobacteria - genetics Proteobacteria - isolation & purification RNA, Ribosomal, 16S - genetics Roseobacter - classification Roseobacter - genetics Roseobacter - isolation & purification rRNA 16S Seawater Seawater - microbiology Sequence Analysis, DNA Starfish - microbiology Symbiosis Waterborne diseases |
| title | A member of the Roseobacter clade, Octadecabacter sp., is the dominant symbiont in the brittle star Amphipholis squamata |
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