Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression
Fatty acids (FAs) play essential roles in regulating differentiation and proliferation by affecting gene expression in various cell types. However, their potential functions in bovine cells remain unclear. Herein, we examine the differentiation and proliferation of bovine skeletal muscle-derived sat...
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| Veröffentlicht in: | Cell and tissue research 2018-08, Vol.373 (2), p.499-508 |
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| creator | Xu, Jiahui Liu, Dan Yin, Hongyan Tong, Huili Li, Shufeng Yan, Yunqin |
| description | Fatty acids (FAs) play essential roles in regulating differentiation and proliferation by affecting gene expression in various cell types. However, their potential functions in bovine cells remain unclear. Herein, we examine the differentiation and proliferation of bovine skeletal muscle-derived satellite cells (MDSCs) after incubation with three types of representative FAs (palmitic acid, oleic acid and docosahexaenoic acid) by western blotting, immunofluorescence assays, flow cytometry analysis and EdU incorporation assays. The myotube fusion rate, myotube length and expression levels of muscle differentiation-related gene myogenin (MYOG) and myosin heavy chain 3 (MYH3) increased significantly, although the FAs did not affect proliferation. Additionally, FA-induced bovine MDSC differentiation increased
ELOVL3
expression and relocation of ELOVL3 to cytoplasmic lipid droplets in the differentiation of bovine MDSCs. Moreover, the effect of FAs on bovine MDSC differentiation was inhibited upon
ELOVL3
downregulation. Collectively, these data indicate that FAs promote bovine MDSC differentiation by regulating
ELOVL3
expression. |
| doi_str_mv | 10.1007/s00441-018-2812-3 |
| format | Article |
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ELOVL3
expression and relocation of ELOVL3 to cytoplasmic lipid droplets in the differentiation of bovine MDSCs. Moreover, the effect of FAs on bovine MDSC differentiation was inhibited upon
ELOVL3
downregulation. Collectively, these data indicate that FAs promote bovine MDSC differentiation by regulating
ELOVL3
expression.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-018-2812-3</identifier><identifier>PMID: 29464364</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetyltransferases - metabolism ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cattle ; Cell differentiation ; Cell Differentiation - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Docosahexaenoic acid ; Down-Regulation - drug effects ; Fatty acids ; Fatty Acids - pharmacology ; Flow cytometry ; Gene expression ; Human Genetics ; Immunofluorescence ; Molecular Medicine ; Monounsaturated fatty acids ; Muscle proteins ; Musculoskeletal system ; Myogenin ; Myosin ; Oleic acid ; Omega 3 fatty acids ; Palmitic acid ; Proteomics ; Regular Article ; Satellite cells ; Satellite Cells, Skeletal Muscle - cytology ; Satellite Cells, Skeletal Muscle - drug effects ; Satellite Cells, Skeletal Muscle - metabolism ; Saturated fatty acids ; Skeletal muscle ; Western blotting</subject><ispartof>Cell and tissue research, 2018-08, Vol.373 (2), p.499-508</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Cell and Tissue Research is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-dfdd4f0325d1d2ecc60cd87a7db39a62cc6178277a20507e70aa3fdbe166e50f3</citedby><cites>FETCH-LOGICAL-c470t-dfdd4f0325d1d2ecc60cd87a7db39a62cc6178277a20507e70aa3fdbe166e50f3</cites><orcidid>0000-0001-6068-3753</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-018-2812-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-018-2812-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29464364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jiahui</creatorcontrib><creatorcontrib>Liu, Dan</creatorcontrib><creatorcontrib>Yin, Hongyan</creatorcontrib><creatorcontrib>Tong, Huili</creatorcontrib><creatorcontrib>Li, Shufeng</creatorcontrib><creatorcontrib>Yan, Yunqin</creatorcontrib><title>Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Fatty acids (FAs) play essential roles in regulating differentiation and proliferation by affecting gene expression in various cell types. However, their potential functions in bovine cells remain unclear. Herein, we examine the differentiation and proliferation of bovine skeletal muscle-derived satellite cells (MDSCs) after incubation with three types of representative FAs (palmitic acid, oleic acid and docosahexaenoic acid) by western blotting, immunofluorescence assays, flow cytometry analysis and EdU incorporation assays. The myotube fusion rate, myotube length and expression levels of muscle differentiation-related gene myogenin (MYOG) and myosin heavy chain 3 (MYH3) increased significantly, although the FAs did not affect proliferation. Additionally, FA-induced bovine MDSC differentiation increased
ELOVL3
expression and relocation of ELOVL3 to cytoplasmic lipid droplets in the differentiation of bovine MDSCs. Moreover, the effect of FAs on bovine MDSC differentiation was inhibited upon
ELOVL3
downregulation. Collectively, these data indicate that FAs promote bovine MDSC differentiation by regulating
ELOVL3
expression.</description><subject>Acetyltransferases - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Docosahexaenoic acid</subject><subject>Down-Regulation - drug effects</subject><subject>Fatty acids</subject><subject>Fatty Acids - pharmacology</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Human Genetics</subject><subject>Immunofluorescence</subject><subject>Molecular Medicine</subject><subject>Monounsaturated fatty acids</subject><subject>Muscle proteins</subject><subject>Musculoskeletal system</subject><subject>Myogenin</subject><subject>Myosin</subject><subject>Oleic acid</subject><subject>Omega 3 fatty acids</subject><subject>Palmitic acid</subject><subject>Proteomics</subject><subject>Regular Article</subject><subject>Satellite cells</subject><subject>Satellite Cells, Skeletal Muscle - cytology</subject><subject>Satellite Cells, Skeletal Muscle - drug effects</subject><subject>Satellite Cells, Skeletal Muscle - metabolism</subject><subject>Saturated fatty acids</subject><subject>Skeletal muscle</subject><subject>Western blotting</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1ks9rVDEQx4Modlv9A7xIQJBeXs2vTd4eS2lVWOhFxVvIJpNtat7LmuQV9783j63WipLDMDOfGWa-GYReUXJGCVHvCiFC0I7QvmM9ZR1_ghZUcNaRXvVP0YJwwjol5dcjdFzKLSFUSLl6jo7YSkjBpVigcGVq3WNjgyt4l9OQKuBNugsj4PINIlQT8TAVG5tvKsQYGmCbxS54DxnGGkwNacSbPc6wnWLzxi2-XF9_WXMMP3YZSmn5F-iZN7HAy3t7gj5fXX66-NCtr99_vDhfd1YoUjvnnROecLZ01DGwVhLremWU2_CVkawFqOqZUoaRJVGgiDHcuw1QKWFJPD9Bp4e-bZvvE5Sqh1Dmgc0IaSqaNeUoZZLJhr75C71NUx7bdDMlpZx1eqC2JoIOo081Gzs31edL0eTlK84bdfYPqj0HQ7BpBB9a_FHB2z8KbsDEelNSnGYty2OQHkCbUykZvN7lMJi815To-Q704Q50uwM934Gea17fbzZtBnC_K359fAPYASgtNW4hP6z-_64_AQ6RvCo</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Xu, Jiahui</creator><creator>Liu, Dan</creator><creator>Yin, Hongyan</creator><creator>Tong, Huili</creator><creator>Li, Shufeng</creator><creator>Yan, Yunqin</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6068-3753</orcidid></search><sort><creationdate>20180801</creationdate><title>Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression</title><author>Xu, Jiahui ; Liu, Dan ; Yin, Hongyan ; Tong, Huili ; Li, Shufeng ; Yan, Yunqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-dfdd4f0325d1d2ecc60cd87a7db39a62cc6178277a20507e70aa3fdbe166e50f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetyltransferases - 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Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jiahui</au><au>Liu, Dan</au><au>Yin, Hongyan</au><au>Tong, Huili</au><au>Li, Shufeng</au><au>Yan, Yunqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>373</volume><issue>2</issue><spage>499</spage><epage>508</epage><pages>499-508</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Fatty acids (FAs) play essential roles in regulating differentiation and proliferation by affecting gene expression in various cell types. However, their potential functions in bovine cells remain unclear. Herein, we examine the differentiation and proliferation of bovine skeletal muscle-derived satellite cells (MDSCs) after incubation with three types of representative FAs (palmitic acid, oleic acid and docosahexaenoic acid) by western blotting, immunofluorescence assays, flow cytometry analysis and EdU incorporation assays. The myotube fusion rate, myotube length and expression levels of muscle differentiation-related gene myogenin (MYOG) and myosin heavy chain 3 (MYH3) increased significantly, although the FAs did not affect proliferation. Additionally, FA-induced bovine MDSC differentiation increased
ELOVL3
expression and relocation of ELOVL3 to cytoplasmic lipid droplets in the differentiation of bovine MDSCs. Moreover, the effect of FAs on bovine MDSC differentiation was inhibited upon
ELOVL3
downregulation. Collectively, these data indicate that FAs promote bovine MDSC differentiation by regulating
ELOVL3
expression.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29464364</pmid><doi>10.1007/s00441-018-2812-3</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6068-3753</orcidid></addata></record> |
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| subjects | Acetyltransferases - metabolism Animals Biomedical and Life Sciences Biomedicine Cattle Cell differentiation Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Docosahexaenoic acid Down-Regulation - drug effects Fatty acids Fatty Acids - pharmacology Flow cytometry Gene expression Human Genetics Immunofluorescence Molecular Medicine Monounsaturated fatty acids Muscle proteins Musculoskeletal system Myogenin Myosin Oleic acid Omega 3 fatty acids Palmitic acid Proteomics Regular Article Satellite cells Satellite Cells, Skeletal Muscle - cytology Satellite Cells, Skeletal Muscle - drug effects Satellite Cells, Skeletal Muscle - metabolism Saturated fatty acids Skeletal muscle Western blotting |
| title | Fatty acids promote bovine skeletal muscle satellite cell differentiation by regulating ELOVL3 expression |
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