Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro
The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi snake venom, on invasion and replication of Toxoplasma gondii in human fibroblasts in vitro. Neuwiedase treatment was done on host cells previously infected with T. gond...
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| Veröffentlicht in: | Experimental parasitology 2008-12, Vol.120 (4), p.391-396 |
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| creator | Bastos, Luciana Machado Júnior, Robson J. Oliveira Silva, Deise Aparecida Oliveira Mineo, José Roberto Vieira, Carlos Ueira Teixeira, David Nascimento Silva Homsi-Brandeburgo, Maria Inês Rodrigues, Veridiana Melo Hamaguchi, Amélia |
| description | The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from
Bothrops neuwiedi snake venom, on invasion and replication of
Toxoplasma gondii in human fibroblasts
in vitro. Neuwiedase treatment was done on host cells previously infected with
T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in
T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in
T. gondii host cell invasion. |
| doi_str_mv | 10.1016/j.exppara.2008.09.008 |
| format | Article |
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Bothrops neuwiedi snake venom, on invasion and replication of
Toxoplasma gondii in human fibroblasts
in vitro. Neuwiedase treatment was done on host cells previously infected with
T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in
T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in
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Bothrops neuwiedi snake venom, on invasion and replication of
Toxoplasma gondii in human fibroblasts
in vitro. Neuwiedase treatment was done on host cells previously infected with
T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in
T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in
T. gondii host cell invasion.</description><subject>Amphibia. Reptilia</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bothrops neuwiedi</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - immunology</subject><subject>Fibroblasts - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human fibroblasts</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Interleukin-8 - biosynthesis</subject><subject>Interleukin-8 - drug effects</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Male</subject><subject>Metalloendopeptidases - toxicity</subject><subject>Mice</subject><subject>Neuwiedase</subject><subject>Protozoa</subject><subject>Snake venom</subject><subject>Toxoplasma - drug effects</subject><subject>Toxoplasma - physiology</subject><subject>Toxoplasma gondii</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><subject>Viper Venoms - toxicity</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEokvhEUC-wKlZbMdJbC4VVOWPVIlLOVsTZ8x6SexgJ0v7NH1VvNoAR06fxvqN55v5iuIlo1tGWfN2v8W7aYIIW06p3FK1zfKo2DCqaMmFUI-LDaVMlEIqcVY8S2lPM8G4eFqcMSl5pSTbFA-34S5MA6QRyPfge-fekWtr0cyJBEs8Lr8c9pDwggAZcYZhCFMMMzqfH4mNYSQfwryLYUp_aEeShx9IDujDeEGCJ_MOifMHSC4X4HsScRqcgflY5zG7ZQRPrOti6LKXPNt5cnBzDM-LJxaGhC9WPS--fby-vfpc3nz99OXq_U1pBBdziYzRDlvDaskqzmTTgVHWVhyalioLtjONUl1bcyoVZZZVtRSdqWvVi5ZxU50Xb07_5uV-LphmPbpkcBjAY1iSzkcWshJNBusTaGJIKaLVU3QjxHvNqD4mo_d6TebYJDVVOkvue7UOWLoR-39daxQZeL0CkAwMNoI3Lv3lsvFWcN5m7vLEYT7HwWHUyTj0Jt895th0H9x_rPwGoDSyRw</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Bastos, Luciana Machado</creator><creator>Júnior, Robson J. Oliveira</creator><creator>Silva, Deise Aparecida Oliveira</creator><creator>Mineo, José Roberto</creator><creator>Vieira, Carlos Ueira</creator><creator>Teixeira, David Nascimento Silva</creator><creator>Homsi-Brandeburgo, Maria Inês</creator><creator>Rodrigues, Veridiana Melo</creator><creator>Hamaguchi, Amélia</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20081201</creationdate><title>Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro</title><author>Bastos, Luciana Machado ; Júnior, Robson J. Oliveira ; Silva, Deise Aparecida Oliveira ; Mineo, José Roberto ; Vieira, Carlos Ueira ; Teixeira, David Nascimento Silva ; Homsi-Brandeburgo, Maria Inês ; Rodrigues, Veridiana Melo ; Hamaguchi, Amélia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-e110be7c158132186bac9ff32a6709fafbc699b75208901f13584bc559d4712c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amphibia. Reptilia</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bothrops neuwiedi</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - immunology</topic><topic>Fibroblasts - parasitology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human fibroblasts</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>Interleukin-8 - biosynthesis</topic><topic>Interleukin-8 - drug effects</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Male</topic><topic>Metalloendopeptidases - toxicity</topic><topic>Mice</topic><topic>Neuwiedase</topic><topic>Protozoa</topic><topic>Snake venom</topic><topic>Toxoplasma - drug effects</topic><topic>Toxoplasma - physiology</topic><topic>Toxoplasma gondii</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><topic>Viper Venoms - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bastos, Luciana Machado</creatorcontrib><creatorcontrib>Júnior, Robson J. 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Oliveira</au><au>Silva, Deise Aparecida Oliveira</au><au>Mineo, José Roberto</au><au>Vieira, Carlos Ueira</au><au>Teixeira, David Nascimento Silva</au><au>Homsi-Brandeburgo, Maria Inês</au><au>Rodrigues, Veridiana Melo</au><au>Hamaguchi, Amélia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>120</volume><issue>4</issue><spage>391</spage><epage>396</epage><pages>391-396</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>The major aim to the present study was to determine the effects of neuwiedase, a metalloproteinase isolated from
Bothrops neuwiedi snake venom, on invasion and replication of
Toxoplasma gondii in human fibroblasts
in vitro. Neuwiedase treatment was done on host cells previously infected with
T. gondii or on parasite before fibroblast infection. When treatments were done after or before infection, infection rates were inhibited in 71% and 61%, respectively. Considering that therapy protocols currently used in
T. gondii infection cause considerable side effects, particularly in immunocompromised individuals and pregnant women, the results of neuwiedase treatment described herein could be taken into account for the development of new synthetic therapeutic agents, mainly due to the capacity of this enzyme to degrade extracellular matrix components, such as laminin, fibronectin and type I collagen, which is important to interfere in
T. gondii host cell invasion.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>18823981</pmid><doi>10.1016/j.exppara.2008.09.008</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4894 |
| ispartof | Experimental parasitology, 2008-12, Vol.120 (4), p.391-396 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Amphibia. Reptilia Animals Biological and medical sciences Bothrops neuwiedi Cell Survival - drug effects Cells, Cultured Dose-Response Relationship, Drug Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - immunology Fibroblasts - parasitology Fundamental and applied biological sciences. Psychology Human fibroblasts Humans Inhibitory Concentration 50 Interleukin-8 - biosynthesis Interleukin-8 - drug effects Life cycle. Host-agent relationship. Pathogenesis Male Metalloendopeptidases - toxicity Mice Neuwiedase Protozoa Snake venom Toxoplasma - drug effects Toxoplasma - physiology Toxoplasma gondii Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Viper Venoms - toxicity |
| title | Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro |
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