Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803
1 Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany 2 Fakultät für Biologie, Albert-Ludwigs-Universität, 79104 Freiburg, Germany Correspondence Dirk Schneider Dirk.Sc...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2007-06, Vol.153 (6), p.1828-1841 |
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| creator | Rupprecht, Eva Gathmann, Sven Fuhrmann, Eva Schneider, Dirk |
| description | 1 Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany
2 Fakultät für Biologie, Albert-Ludwigs-Universität, 79104 Freiburg, Germany
Correspondence Dirk Schneider Dirk.Schneider{at}biochemie.uni-freiburg.de
Multiple dnaK genes appear to be common in cyanobacteria; the function of the encoded proteins is, however, still elusive. To characterize the dnaK gene family from the cyanobacterium Synechocystis sp. PCC 6803 in detail, genetic analyses were combined with analyses of the expression and localization patterns of the three encoded proteins. While significant expression of all three genes was found, the results obtained clearly indicate physiological differences of the three proteins in vivo , and DnaK2 seems to have a key function in Synechocystis . Expression of DnaK3 appears also to be as essential as expression of DnaK2, whereas the dnaK1 gene was deleted without resulting in any distorted phenotype. In line with a suggested privileged function, expression of DnaK2 altered most significantly after heat shock. |
| doi_str_mv | 10.1099/mic.0.2007/005876-0 |
| format | Article |
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2 Fakultät für Biologie, Albert-Ludwigs-Universität, 79104 Freiburg, Germany
Correspondence Dirk Schneider Dirk.Schneider{at}biochemie.uni-freiburg.de
Multiple dnaK genes appear to be common in cyanobacteria; the function of the encoded proteins is, however, still elusive. To characterize the dnaK gene family from the cyanobacterium Synechocystis sp. PCC 6803 in detail, genetic analyses were combined with analyses of the expression and localization patterns of the three encoded proteins. While significant expression of all three genes was found, the results obtained clearly indicate physiological differences of the three proteins in vivo , and DnaK2 seems to have a key function in Synechocystis . Expression of DnaK3 appears also to be as essential as expression of DnaK2, whereas the dnaK1 gene was deleted without resulting in any distorted phenotype. In line with a suggested privileged function, expression of DnaK2 altered most significantly after heat shock.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.2007/005876-0</identifier><identifier>PMID: 17526840</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Artificial Gene Fusion ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Blotting, Western ; Cell Fractionation ; Cell Membrane - chemistry ; Cyanobacteria ; Cyanophyta ; Cytosol - chemistry ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression ; Genes, Bacterial ; Genes, Essential ; Genes, Reporter ; Genetics ; Heat-Shock Proteins - biosynthesis ; Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - biosynthesis ; HSP70 Heat-Shock Proteins - genetics ; Luciferases - analysis ; Luciferases - genetics ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Insertional ; Phylogeny ; Sequence Alignment ; Synechocystis ; Synechocystis - genetics ; Synechocystis - physiology</subject><ispartof>Microbiology (Society for General Microbiology), 2007-06, Vol.153 (6), p.1828-1841</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-50dc644393d7e685cd6211fa545c31af270221a7188be06f69ed5ae4b2ac8aee3</citedby><cites>FETCH-LOGICAL-c474t-50dc644393d7e685cd6211fa545c31af270221a7188be06f69ed5ae4b2ac8aee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18849095$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17526840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rupprecht, Eva</creatorcontrib><creatorcontrib>Gathmann, Sven</creatorcontrib><creatorcontrib>Fuhrmann, Eva</creatorcontrib><creatorcontrib>Schneider, Dirk</creatorcontrib><title>Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>1 Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany
2 Fakultät für Biologie, Albert-Ludwigs-Universität, 79104 Freiburg, Germany
Correspondence Dirk Schneider Dirk.Schneider{at}biochemie.uni-freiburg.de
Multiple dnaK genes appear to be common in cyanobacteria; the function of the encoded proteins is, however, still elusive. To characterize the dnaK gene family from the cyanobacterium Synechocystis sp. PCC 6803 in detail, genetic analyses were combined with analyses of the expression and localization patterns of the three encoded proteins. While significant expression of all three genes was found, the results obtained clearly indicate physiological differences of the three proteins in vivo , and DnaK2 seems to have a key function in Synechocystis . Expression of DnaK3 appears also to be as essential as expression of DnaK2, whereas the dnaK1 gene was deleted without resulting in any distorted phenotype. In line with a suggested privileged function, expression of DnaK2 altered most significantly after heat shock.</description><subject>Amino Acid Sequence</subject><subject>Artificial Gene Fusion</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Fractionation</subject><subject>Cell Membrane - chemistry</subject><subject>Cyanobacteria</subject><subject>Cyanophyta</subject><subject>Cytosol - chemistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Genes, Essential</subject><subject>Genes, Reporter</subject><subject>Genetics</subject><subject>Heat-Shock Proteins - biosynthesis</subject><subject>Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - biosynthesis</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>Luciferases - analysis</subject><subject>Luciferases - genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Phylogeny</subject><subject>Sequence Alignment</subject><subject>Synechocystis</subject><subject>Synechocystis - genetics</subject><subject>Synechocystis - physiology</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuL1UAQhYMozkN_gSC9UZhF7lS_O0u5PnFAwXHddDqVSUvSuXYnaP69fbkXZumqCuo7p6hTVfWKwo5C09xOwe9gxwD0LYA0WtXwpLqkQsmagYGnpecSajCaXVRXOf8CKEOgz6sLqiVTRsBlFe6HhEi60PeYMC7kfXRfySHNC4aYiUtI-jX6JczRjeNG8O8hYc7YkRDJMiDxm4tz6_yCKawT-bFF9MPst7yETPJhR77v90QZ4C-qZ70bM7481-vq58cP9_vP9d23T1_27-5qL7RYagmdV0LwhncalZG-U4zS3kkhPaeuZxoYo05TY1oE1asGO-lQtMx54xD5dfX25FuO-L1iXuwUssdxdBHnNdsSmJCg-H9B2jSacWEKyE-gT3POCXt7SGFyabMU7PEVRegtHJ21Pb3CQlG9Ptuv7YTdo-acfQHenAGXvRv75KIP-ZEzRjTQyMLdnLghPAx_QkL7gLFsTHMb5uNqKrlVhWeG_wPx9KBs</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Rupprecht, Eva</creator><creator>Gathmann, Sven</creator><creator>Fuhrmann, Eva</creator><creator>Schneider, Dirk</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20070601</creationdate><title>Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803</title><author>Rupprecht, Eva ; Gathmann, Sven ; Fuhrmann, Eva ; Schneider, Dirk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-50dc644393d7e685cd6211fa545c31af270221a7188be06f69ed5ae4b2ac8aee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amino Acid Sequence</topic><topic>Artificial Gene Fusion</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Fractionation</topic><topic>Cell Membrane - chemistry</topic><topic>Cyanobacteria</topic><topic>Cyanophyta</topic><topic>Cytosol - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Genes, Essential</topic><topic>Genes, Reporter</topic><topic>Genetics</topic><topic>Heat-Shock Proteins - biosynthesis</topic><topic>Heat-Shock Proteins - genetics</topic><topic>HSP70 Heat-Shock Proteins - biosynthesis</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>Luciferases - analysis</topic><topic>Luciferases - genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Phylogeny</topic><topic>Sequence Alignment</topic><topic>Synechocystis</topic><topic>Synechocystis - genetics</topic><topic>Synechocystis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rupprecht, Eva</creatorcontrib><creatorcontrib>Gathmann, Sven</creatorcontrib><creatorcontrib>Fuhrmann, Eva</creatorcontrib><creatorcontrib>Schneider, Dirk</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rupprecht, Eva</au><au>Gathmann, Sven</au><au>Fuhrmann, Eva</au><au>Schneider, Dirk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>153</volume><issue>6</issue><spage>1828</spage><epage>1841</epage><pages>1828-1841</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>1 Institut für Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany
2 Fakultät für Biologie, Albert-Ludwigs-Universität, 79104 Freiburg, Germany
Correspondence Dirk Schneider Dirk.Schneider{at}biochemie.uni-freiburg.de
Multiple dnaK genes appear to be common in cyanobacteria; the function of the encoded proteins is, however, still elusive. To characterize the dnaK gene family from the cyanobacterium Synechocystis sp. PCC 6803 in detail, genetic analyses were combined with analyses of the expression and localization patterns of the three encoded proteins. While significant expression of all three genes was found, the results obtained clearly indicate physiological differences of the three proteins in vivo , and DnaK2 seems to have a key function in Synechocystis . Expression of DnaK3 appears also to be as essential as expression of DnaK2, whereas the dnaK1 gene was deleted without resulting in any distorted phenotype. In line with a suggested privileged function, expression of DnaK2 altered most significantly after heat shock.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>17526840</pmid><doi>10.1099/mic.0.2007/005876-0</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Artificial Gene Fusion Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacteriology Biological and medical sciences Blotting, Western Cell Fractionation Cell Membrane - chemistry Cyanobacteria Cyanophyta Cytosol - chemistry Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Genes, Bacterial Genes, Essential Genes, Reporter Genetics Heat-Shock Proteins - biosynthesis Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - biosynthesis HSP70 Heat-Shock Proteins - genetics Luciferases - analysis Luciferases - genetics Microbiology Molecular Sequence Data Mutagenesis, Insertional Phylogeny Sequence Alignment Synechocystis Synechocystis - genetics Synechocystis - physiology |
| title | Three different DnaK proteins are functionally expressed in the cyanobacterium Synechocystis sp. PCC 6803 |
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