CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera
C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was...
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| Veröffentlicht in: | Developmental and comparative immunology 2018-07, Vol.84, p.142-152 |
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| creator | Cheng, Yang Lin, Zhe Wang, Ju-Mei Xing, Long-Sheng Xiong, Guang-Hua Zou, Zhen |
| description | C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph. Furthermore, depletion of CTL14 using dsRNA led to dramatic decrease in the expression level of PPO1. Additionally, CTL14 depleted H. armigera decreased the resistance to fungal challenge. Taken together, our study showed the direct involvement of CTL14 in the anti-fungal immunity of H. armigera, which further explained the stronger immune responses in the fifth instar compared to the second instar larvae.
•CTL14 is induced by fungi in the fifth instar larvae, not in the second instar.•Recombinant CTL14 is able to bind and agglutinate Beauveria bassiana.•CTL14 modulates the expression of SPs and serpin to regulate melanization pathway.•CTL14-rich hemocytes are capable of phagocytosing fungal conidia. |
| doi_str_mv | 10.1016/j.dci.2018.02.010 |
| format | Article |
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•CTL14 is induced by fungi in the fifth instar larvae, not in the second instar.•Recombinant CTL14 is able to bind and agglutinate Beauveria bassiana.•CTL14 modulates the expression of SPs and serpin to regulate melanization pathway.•CTL14-rich hemocytes are capable of phagocytosing fungal conidia.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2018.02.010</identifier><identifier>PMID: 29453998</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Anti-Fungal immunity ; Beauveria bassiana ; C-type lectin ; Cotton ; Cytotoxicity ; Double-stranded RNA ; Entomopathogen ; Fungal infections ; Fungi ; Fungicides ; Gene expression ; Helicoverpa armigera ; Hemolymph ; Immune response ; Immunity ; Immunology ; Immunoprecipitation ; Innate immunity ; Insects ; Larvae ; Lectins ; Lymphocytes T ; Pathogens ; Pattern recognition ; Pattern recognition receptors ; Proteinase ; Proteinase inhibitors ; Proteins ; Receptors ; Serine ; Serine proteinase ; Serine proteinase inhibitors ; Vitellogenin</subject><ispartof>Developmental and comparative immunology, 2018-07, Vol.84, p.142-152</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Jul 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-c262d1e668d0d2683851fc199963580ba5f5e718a00dc3d1e4911812bf6c09923</citedby><cites>FETCH-LOGICAL-c424t-c262d1e668d0d2683851fc199963580ba5f5e718a00dc3d1e4911812bf6c09923</cites><orcidid>0000-0003-3550-7656</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X17306468$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29453998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Yang</creatorcontrib><creatorcontrib>Lin, Zhe</creatorcontrib><creatorcontrib>Wang, Ju-Mei</creatorcontrib><creatorcontrib>Xing, Long-Sheng</creatorcontrib><creatorcontrib>Xiong, Guang-Hua</creatorcontrib><creatorcontrib>Zou, Zhen</creatorcontrib><title>CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph. Furthermore, depletion of CTL14 using dsRNA led to dramatic decrease in the expression level of PPO1. Additionally, CTL14 depleted H. armigera decreased the resistance to fungal challenge. Taken together, our study showed the direct involvement of CTL14 in the anti-fungal immunity of H. armigera, which further explained the stronger immune responses in the fifth instar compared to the second instar larvae.
•CTL14 is induced by fungi in the fifth instar larvae, not in the second instar.•Recombinant CTL14 is able to bind and agglutinate Beauveria bassiana.•CTL14 modulates the expression of SPs and serpin to regulate melanization pathway.•CTL14-rich hemocytes are capable of phagocytosing fungal conidia.</description><subject>Anti-Fungal immunity</subject><subject>Beauveria bassiana</subject><subject>C-type lectin</subject><subject>Cotton</subject><subject>Cytotoxicity</subject><subject>Double-stranded RNA</subject><subject>Entomopathogen</subject><subject>Fungal infections</subject><subject>Fungi</subject><subject>Fungicides</subject><subject>Gene expression</subject><subject>Helicoverpa armigera</subject><subject>Hemolymph</subject><subject>Immune response</subject><subject>Immunity</subject><subject>Immunology</subject><subject>Immunoprecipitation</subject><subject>Innate immunity</subject><subject>Insects</subject><subject>Larvae</subject><subject>Lectins</subject><subject>Lymphocytes T</subject><subject>Pathogens</subject><subject>Pattern recognition</subject><subject>Pattern recognition receptors</subject><subject>Proteinase</subject><subject>Proteinase inhibitors</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Serine</subject><subject>Serine proteinase</subject><subject>Serine proteinase inhibitors</subject><subject>Vitellogenin</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kUGL1DAYhoMo7rj6A7xIwIuHbf2SNm2Cp2VQVxjwsoK3kEm-DhnaZkzSkb36y02Z1YMHc_k-wvO-hDyEvGZQM2Dd-2PtrK85MFkDr4HBE7JhslcVgFRPyQZYK6oGxPcr8iKlI5QjGTwnV1y1olFKbsiv7f2OtTfU0Ig2HGaffZjXHU85ROpnt1h0ZdLRZKQpmwOWNZ4N3tApuGW9TtTM2VfDMh_MSP00LaXnYQ3ZkHPp24dx_BniRO9w9DacMZ4MNXHyB4zmJXk2mDHhq8d5Tb59-ni_vat2Xz9_2d7uKtvyNleWd9wx7DrpwPFONlKwwTKlVNcICXsjBoE9kwbA2aaQrWJMMr4fOgtK8eaavLv0nmL4sWDKevLJ4jiaGcOSNAdooBV9DwV9-w96DEucy-sK1TeCNaLtC8UulI0hpYiDPkU_mfigGehVkD7qIkivgjRwXQSVzJvH5mU_ofub-GOkAB8uAJavOHuMOlmPc5Hgi5WsXfD_qf8NHBugcw</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Cheng, Yang</creator><creator>Lin, Zhe</creator><creator>Wang, Ju-Mei</creator><creator>Xing, Long-Sheng</creator><creator>Xiong, Guang-Hua</creator><creator>Zou, Zhen</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3550-7656</orcidid></search><sort><creationdate>20180701</creationdate><title>CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera</title><author>Cheng, Yang ; Lin, Zhe ; Wang, Ju-Mei ; Xing, Long-Sheng ; Xiong, Guang-Hua ; Zou, Zhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-c262d1e668d0d2683851fc199963580ba5f5e718a00dc3d1e4911812bf6c09923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anti-Fungal immunity</topic><topic>Beauveria bassiana</topic><topic>C-type lectin</topic><topic>Cotton</topic><topic>Cytotoxicity</topic><topic>Double-stranded RNA</topic><topic>Entomopathogen</topic><topic>Fungal infections</topic><topic>Fungi</topic><topic>Fungicides</topic><topic>Gene expression</topic><topic>Helicoverpa armigera</topic><topic>Hemolymph</topic><topic>Immune response</topic><topic>Immunity</topic><topic>Immunology</topic><topic>Immunoprecipitation</topic><topic>Innate immunity</topic><topic>Insects</topic><topic>Larvae</topic><topic>Lectins</topic><topic>Lymphocytes T</topic><topic>Pathogens</topic><topic>Pattern recognition</topic><topic>Pattern recognition receptors</topic><topic>Proteinase</topic><topic>Proteinase inhibitors</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Serine</topic><topic>Serine proteinase</topic><topic>Serine proteinase inhibitors</topic><topic>Vitellogenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Yang</creatorcontrib><creatorcontrib>Lin, Zhe</creatorcontrib><creatorcontrib>Wang, Ju-Mei</creatorcontrib><creatorcontrib>Xing, Long-Sheng</creatorcontrib><creatorcontrib>Xiong, Guang-Hua</creatorcontrib><creatorcontrib>Zou, Zhen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Yang</au><au>Lin, Zhe</au><au>Wang, Ju-Mei</au><au>Xing, Long-Sheng</au><au>Xiong, Guang-Hua</au><au>Zou, Zhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>84</volume><spage>142</spage><epage>152</epage><pages>142-152</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>C-type lectin (CTL) is usually considered as pattern recognition receptors in insect innate immunity. Here we found that CTL14 of Helicoverpa armigera was only activated in the fifth instar larvae not in the second instar by entomopathogen Beauveria bassiana infection. Recombinant CTL14 protein was found to form aggregates with zymosan and B. bassiana in vitro. Immunoprecipitation studies demonstrated that CTL14 interacted with serine proteinases (SP), serine proteinase inhibitor (serpin), prophenoloxidases (PPO) and vitellogenin (Vg) in the larval hemolymph. Furthermore, depletion of CTL14 using dsRNA led to dramatic decrease in the expression level of PPO1. Additionally, CTL14 depleted H. armigera decreased the resistance to fungal challenge. Taken together, our study showed the direct involvement of CTL14 in the anti-fungal immunity of H. armigera, which further explained the stronger immune responses in the fifth instar compared to the second instar larvae.
•CTL14 is induced by fungi in the fifth instar larvae, not in the second instar.•Recombinant CTL14 is able to bind and agglutinate Beauveria bassiana.•CTL14 modulates the expression of SPs and serpin to regulate melanization pathway.•CTL14-rich hemocytes are capable of phagocytosing fungal conidia.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>29453998</pmid><doi>10.1016/j.dci.2018.02.010</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3550-7656</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Fungal immunity Beauveria bassiana C-type lectin Cotton Cytotoxicity Double-stranded RNA Entomopathogen Fungal infections Fungi Fungicides Gene expression Helicoverpa armigera Hemolymph Immune response Immunity Immunology Immunoprecipitation Innate immunity Insects Larvae Lectins Lymphocytes T Pathogens Pattern recognition Pattern recognition receptors Proteinase Proteinase inhibitors Proteins Receptors Serine Serine proteinase Serine proteinase inhibitors Vitellogenin |
| title | CTL14, a recognition receptor induced in late stage larvae, modulates anti-fungal immunity in cotton bollworm Helicoverpa armigera |
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