Single organelle dynamics linked to 3D structure by correlative live‐cell imaging and 3D electron microscopy

Live‐cell correlative light‐electron microscopy (live‐cell‐CLEM) integrates live movies with the corresponding electron microscopy (EM) image, but a major challenge is to relate the dynamic characteristics of single organelles to their 3‐dimensional (3D) ultrastructure. Here, we introduce focused io...

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Veröffentlicht in:	Traffic (Copenhagen, Denmark) Denmark), 2018-05, Vol.19 (5), p.354-369
Hauptverfasser:	Fermie, Job, Liv, Nalan, ten Brink, Corlinda, van Donselaar, Elly G., Müller, Wally H., Schieber, Nicole L., Schwab, Yannick, Gerritsen, Hans C., Klumperman, Judith
Format:	Artikel
Sprache:	eng
Schlagworte:	correlative light‐electron microscopy Data processing Electron Microscope Tomography - methods endolysosomal system Endoplasmic reticulum focused ion beam scanning electron microscopy Green fluorescent protein HeLa Cells Humans Ion beams Lysosomal Membrane Proteins - metabolism Lysosomes - metabolism Lysosomes - ultrastructure Membrane proteins Membrane trafficking Mitochondria Optical Imaging - methods Organelle Biogenesis organelle dynamics Organelles Physical characteristics Scanning electron microscopy time‐lapse microscopy Ultrastructure volume electron microscopy
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