Biochemical Characterization of SFC-1, a Class A Carbapenem-Hydrolyzing {szligbeta}-Lactamase

Biochemical Characterization of SFC-1, a Class A Carbapenem-Hydrolyzing {szligbeta}-Lactamase

The carbapenem-hydrolyzing {szligbeta}-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrol...

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Veröffentlicht in:Antimicrobial agents and chemotherapy 2007-12, Vol.51 (12), p.4512-4514
Hauptverfasser: Fonseca, Fatima, Sarmento, Ana Cristina, Henriques, Isabel, Samyn, Bart, van Beeumen, Jozef, Domingues, Pedro, Domingues, Maria Rosario, Saavedra, Maria Jose, Correia, Antonio
Format: Artikel
Sprache:eng
Schlagworte:
Escherichia coli
Serratia fonticola
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Zusammenfassung:The carbapenem-hydrolyzing {szligbeta}-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
ISSN:0066-4804
1098-6596