Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression
The use of sugar‐functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well‐known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes,...
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| Veröffentlicht in: | Macromolecular bioscience 2018-02, Vol.18 (2), p.n/a |
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| creator | Albuquerque, Lindomar J. C. Alavarse, Alex C. Carlan da Silva, Maria C. Zilse, Morgana S. Barth, Maitê T. Bellettini, Ismael C. Giacomelli, Fernando C. |
| description | The use of sugar‐functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well‐known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency. Accordingly, the preparation of lower‐cytotoxicity polyplexes while maintaining gene expression, which is highly relevant to the field, is demonstrated.
The substitution of primary amine groups in branched polyethyleneimine chains by lactose residues enables the preparation of lower‐cytotoxicity polyplexes that are taken up by cells to a greater extent while maintaining the levels of gene expression. |
| doi_str_mv | 10.1002/mabi.201700299 |
| format | Article |
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The substitution of primary amine groups in branched polyethyleneimine chains by lactose residues enables the preparation of lower‐cytotoxicity polyplexes that are taken up by cells to a greater extent while maintaining the levels of gene expression.</description><subject>Animals</subject><subject>Benzoxazoles - chemistry</subject><subject>Cell Death</subject><subject>Cell Survival</subject><subject>Condensation polymerization</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>Fluorescence</subject><subject>gene delivery</subject><subject>Gene Expression</subject><subject>Gene transfer</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Vectors - metabolism</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Lactose</subject><subject>Macaca mulatta</subject><subject>Polyethyleneimine</subject><subject>Polyethyleneimine - chemistry</subject><subject>Quinolinium Compounds - chemistry</subject><subject>Scattering, Radiation</subject><subject>Sugar</subject><subject>sugar functionalization</subject><subject>Sugars - chemistry</subject><subject>sweet polyplexes</subject><subject>Toxicity</subject><subject>Transfection</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFPGzEQha2qiFDg2mNlqZdeEmzv2rvmBiEFpCAQaXtdeb1jMNrEqe0l2WP_eQ0JQeqlh9GMR9-8Gfkh9JmSESWEncxVbUeM0CI9pPyADqigYsip5B93dVkM0KcQnkjCSsn20YDJPBO0JAfoz2wFEPEv0NF5bFJcwgLwBbT2GXx_iuMj4Fn3oHzqaedVtG6BncF3ru2XLawh4HtoOp3yuI8uurXVNvZ4ZeMjVvhctWqhocETY9IOnIZfF0zWSw8hJLEjtGdUG-B4mw_Rz--TH-Or4fT28np8Nh3qnBI5pIzUwBhoEIbRhhsJLNeC14opQaQSZaGIqAsupVIaeFk2ecMEKzKacVPy7BB92-guvfvdQYjV3AYNbboPXBcqlr5H0jJnMqFf_0GfXOcX6bpXKkvBWaJGG0p7F4IHUy29nSvfV5RUL-ZUL-ZUO3PSwJetbFfPodnhb24kQG6AlW2h_49cdXN2fv0u_heWdZtB</recordid><startdate>201802</startdate><enddate>201802</enddate><creator>Albuquerque, Lindomar J. C.</creator><creator>Alavarse, Alex C.</creator><creator>Carlan da Silva, Maria C.</creator><creator>Zilse, Morgana S.</creator><creator>Barth, Maitê T.</creator><creator>Bellettini, Ismael C.</creator><creator>Giacomelli, Fernando C.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1443-1795</orcidid><orcidid>https://orcid.org/0000-0002-6872-9354</orcidid></search><sort><creationdate>201802</creationdate><title>Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression</title><author>Albuquerque, Lindomar J. C. ; Alavarse, Alex C. ; Carlan da Silva, Maria C. ; Zilse, Morgana S. ; Barth, Maitê T. ; Bellettini, Ismael C. ; Giacomelli, Fernando C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4109-120be22ece6f21d5f9e24c65ba2a609a687a06b7599aace588d4d26273135f853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Benzoxazoles - chemistry</topic><topic>Cell Death</topic><topic>Cell Survival</topic><topic>Condensation polymerization</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>Fluorescence</topic><topic>gene delivery</topic><topic>Gene Expression</topic><topic>Gene transfer</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Vectors - metabolism</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Lactose</topic><topic>Macaca mulatta</topic><topic>Polyethyleneimine</topic><topic>Polyethyleneimine - chemistry</topic><topic>Quinolinium Compounds - chemistry</topic><topic>Scattering, Radiation</topic><topic>Sugar</topic><topic>sugar functionalization</topic><topic>Sugars - chemistry</topic><topic>sweet polyplexes</topic><topic>Toxicity</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albuquerque, Lindomar J. C.</creatorcontrib><creatorcontrib>Alavarse, Alex C.</creatorcontrib><creatorcontrib>Carlan da Silva, Maria C.</creatorcontrib><creatorcontrib>Zilse, Morgana S.</creatorcontrib><creatorcontrib>Barth, Maitê T.</creatorcontrib><creatorcontrib>Bellettini, Ismael C.</creatorcontrib><creatorcontrib>Giacomelli, Fernando C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albuquerque, Lindomar J. C.</au><au>Alavarse, Alex C.</au><au>Carlan da Silva, Maria C.</au><au>Zilse, Morgana S.</au><au>Barth, Maitê T.</au><au>Bellettini, Ismael C.</au><au>Giacomelli, Fernando C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol Biosci</addtitle><date>2018-02</date><risdate>2018</risdate><volume>18</volume><issue>2</issue><epage>n/a</epage><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>The use of sugar‐functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well‐known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency. Accordingly, the preparation of lower‐cytotoxicity polyplexes while maintaining gene expression, which is highly relevant to the field, is demonstrated.
The substitution of primary amine groups in branched polyethyleneimine chains by lactose residues enables the preparation of lower‐cytotoxicity polyplexes that are taken up by cells to a greater extent while maintaining the levels of gene expression.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29436180</pmid><doi>10.1002/mabi.201700299</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1443-1795</orcidid><orcidid>https://orcid.org/0000-0002-6872-9354</orcidid></addata></record> |
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| subjects | Animals Benzoxazoles - chemistry Cell Death Cell Survival Condensation polymerization Cytotoxicity Deoxyribonucleic acid DNA DNA - metabolism Fluorescence gene delivery Gene Expression Gene transfer Gene Transfer Techniques Genetic Vectors - metabolism Green Fluorescent Proteins - metabolism HeLa Cells Humans Lactose Macaca mulatta Polyethyleneimine Polyethyleneimine - chemistry Quinolinium Compounds - chemistry Scattering, Radiation Sugar sugar functionalization Sugars - chemistry sweet polyplexes Toxicity Transfection |
| title | Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression |
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