An efficient screening method for purifying and crystallizing membrane proteins using modified clear-native PAGE

Membrane proteins, such as G-protein coupled receptors, control communication between cells and their environments and are indispensable for many cellular functions. Nevertheless, structural studies on membrane proteins lag behind those on water-soluble proteins, due to their low structural stabilit...

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Veröffentlicht in:	Analytical biochemistry 2018-05, Vol.548, p.7-14
Hauptverfasser:	Suzuki, Nanao, Takamuku, Yuuki, Asakawa, Tomohiro, Inai, Makoto, Hino, Tomoya, Iwata, So, Kan, Toshiyuki, Murata, Takeshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Clear-native PAGE Crystallization Crystallography, X-Ray - methods G protein-coupled receptor Humans Membrane proteins Native Polyacrylamide Gel Electrophoresis - methods Purification Receptor, Adenosine A2A - chemistry Receptor, Adenosine A2A - isolation & purification Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Stability
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