New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection
WPMP-2 is an acid polysaccharide isolated from Polygonum multiflorum, which demonstrated excellent immunomodulatory activity. In order to reduce immunosuppression of 5-fluorouracil (5-Fu), WPMP-2 was utilized as a macromolecular carrier to conjugate the 5-Fu derivatives 5-fluorouracil-1-acetic acid...
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| Veröffentlicht in: | International journal of biological macromolecules 2018-09, Vol.116, p.1310-1316 |
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| container_title | International journal of biological macromolecules |
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| creator | Zhang, Qing Xu, Yi Lv, Junjiang Cheng, Mengxia Wu, Ying Cao, Kun Zhang, Xiaofeng Mou, Xiuni Fan, Qi |
| description | WPMP-2 is an acid polysaccharide isolated from Polygonum multiflorum, which demonstrated excellent immunomodulatory activity. In order to reduce immunosuppression of 5-fluorouracil (5-Fu), WPMP-2 was utilized as a macromolecular carrier to conjugate the 5-Fu derivatives 5-fluorouracil-1-acetic acid (5-FUAC) through ester bond. The conjugate showed controlled drug release behaviour in vitro at 37°C in phosphate buffer (pH7.4), and only 5-FUAC was detected in the media. The cytotoxicity test in vitro showed that the conjugate exhibited different cytotoxicity to HepG-2 and HT-29 cells. In addition, immunization study in vivo illustrated that the conjugate displayed immunoprotective effect by mitigating inhibition and damage effects of 5-Fu on secretion of cytokines, proliferation of splenocytes, and phagocytosis of peritoneal macrophages. It was indicated that the conjugation of 5-Fu and WPMP-2 could be a potential double effective drug delivery system. |
| doi_str_mv | 10.1016/j.ijbiomac.2018.02.052 |
| format | Article |
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In order to reduce immunosuppression of 5-fluorouracil (5-Fu), WPMP-2 was utilized as a macromolecular carrier to conjugate the 5-Fu derivatives 5-fluorouracil-1-acetic acid (5-FUAC) through ester bond. The conjugate showed controlled drug release behaviour in vitro at 37°C in phosphate buffer (pH7.4), and only 5-FUAC was detected in the media. The cytotoxicity test in vitro showed that the conjugate exhibited different cytotoxicity to HepG-2 and HT-29 cells. In addition, immunization study in vivo illustrated that the conjugate displayed immunoprotective effect by mitigating inhibition and damage effects of 5-Fu on secretion of cytokines, proliferation of splenocytes, and phagocytosis of peritoneal macrophages. It was indicated that the conjugation of 5-Fu and WPMP-2 could be a potential double effective drug delivery system.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2018.02.052</identifier><identifier>PMID: 29432832</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Controlled release ; Cytokines - metabolism ; Delayed-Action Preparations - chemistry ; Delayed-Action Preparations - pharmacokinetics ; Delayed-Action Preparations - pharmacology ; Drug Carriers - chemistry ; Drug Carriers - pharmacokinetics ; Drug Carriers - pharmacology ; Fallopia multiflora - chemistry ; Female ; Fluorouracil - chemistry ; Fluorouracil - pharmacokinetics ; Fluorouracil - pharmacology ; Hep G2 Cells ; Humans ; Hydrogen-Ion Concentration ; Immunoprotection ; Macrophages, Peritoneal - metabolism ; Macrophages, Peritoneal - pathology ; Mice ; Neoplasms, Experimental - drug therapy ; Neoplasms, Experimental - metabolism ; Neoplasms, Experimental - pathology ; Polygonum multiflorum polysaccharide ; Polysaccharides - chemistry ; Polysaccharides - pharmacokinetics ; Polysaccharides - pharmacology ; Spleen - metabolism ; Spleen - pathology</subject><ispartof>International journal of biological macromolecules, 2018-09, Vol.116, p.1310-1316</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-91bb801ff6265496620fc9085ce45a9c3ee7816d922e9021cad932238e9960953</citedby><cites>FETCH-LOGICAL-c368t-91bb801ff6265496620fc9085ce45a9c3ee7816d922e9021cad932238e9960953</cites><orcidid>0000-0002-4698-0193</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2018.02.052$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29432832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qing</creatorcontrib><creatorcontrib>Xu, Yi</creatorcontrib><creatorcontrib>Lv, Junjiang</creatorcontrib><creatorcontrib>Cheng, Mengxia</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Cao, Kun</creatorcontrib><creatorcontrib>Zhang, Xiaofeng</creatorcontrib><creatorcontrib>Mou, Xiuni</creatorcontrib><creatorcontrib>Fan, Qi</creatorcontrib><title>New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>WPMP-2 is an acid polysaccharide isolated from Polygonum multiflorum, which demonstrated excellent immunomodulatory activity. In order to reduce immunosuppression of 5-fluorouracil (5-Fu), WPMP-2 was utilized as a macromolecular carrier to conjugate the 5-Fu derivatives 5-fluorouracil-1-acetic acid (5-FUAC) through ester bond. The conjugate showed controlled drug release behaviour in vitro at 37°C in phosphate buffer (pH7.4), and only 5-FUAC was detected in the media. The cytotoxicity test in vitro showed that the conjugate exhibited different cytotoxicity to HepG-2 and HT-29 cells. In addition, immunization study in vivo illustrated that the conjugate displayed immunoprotective effect by mitigating inhibition and damage effects of 5-Fu on secretion of cytokines, proliferation of splenocytes, and phagocytosis of peritoneal macrophages. It was indicated that the conjugation of 5-Fu and WPMP-2 could be a potential double effective drug delivery system.</description><subject>Animals</subject><subject>Controlled release</subject><subject>Cytokines - metabolism</subject><subject>Delayed-Action Preparations - chemistry</subject><subject>Delayed-Action Preparations - pharmacokinetics</subject><subject>Delayed-Action Preparations - pharmacology</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - pharmacokinetics</subject><subject>Drug Carriers - pharmacology</subject><subject>Fallopia multiflora - chemistry</subject><subject>Female</subject><subject>Fluorouracil - chemistry</subject><subject>Fluorouracil - pharmacokinetics</subject><subject>Fluorouracil - pharmacology</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoprotection</subject><subject>Macrophages, Peritoneal - metabolism</subject><subject>Macrophages, Peritoneal - pathology</subject><subject>Mice</subject><subject>Neoplasms, Experimental - drug therapy</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Polygonum multiflorum polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - pharmacokinetics</subject><subject>Polysaccharides - pharmacology</subject><subject>Spleen - metabolism</subject><subject>Spleen - pathology</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcGOFCEQJUbjjqu_sOHopdsCphm4aTaummzUg54JQxfKBJoRGs36E_6yTGbXq6eqVL16L_UeIVcMRgZMvjqM4bAPOVk3cmBqBD7CxB-RDVM7PQCAeEw2wLZsUEzABXlW66FP5cTUU3LB9VZwJfiG_PmIv2hbQwy_7RryQrOnn3O8-5aXlmhqcQ0-5tL7Y59W69x3W8KM1FbaxUtOOaJr0RbqbCkBy4lhGnxsueRWrAuR-ty3eVlLjhFnWjCirZ1imWlIqS35WPKK7qT_nDzxNlZ8cV8vydebt1-u3w-3n959uH5zOzgh1Tpott8rYN5LLqetlpKDdxrU5HA7We0E4k4xOWvOUQNnzs5acC4Uai1BT-KSvDzzdukfDetqUqgOY7QL5lYNB2CaCbVjHSrP0P5trQW9OZaQbLkzDMwpDHMwD2GYUxgGuOlh9MOre422Tzj_O3twvwNenwHYP_3ZvTPVBVwczqF0O8ycw_80_gL6qqHe</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Zhang, Qing</creator><creator>Xu, Yi</creator><creator>Lv, Junjiang</creator><creator>Cheng, Mengxia</creator><creator>Wu, Ying</creator><creator>Cao, Kun</creator><creator>Zhang, Xiaofeng</creator><creator>Mou, Xiuni</creator><creator>Fan, Qi</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4698-0193</orcidid></search><sort><creationdate>201809</creationdate><title>New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection</title><author>Zhang, Qing ; Xu, Yi ; Lv, Junjiang ; Cheng, Mengxia ; Wu, Ying ; Cao, Kun ; Zhang, Xiaofeng ; Mou, Xiuni ; Fan, Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-91bb801ff6265496620fc9085ce45a9c3ee7816d922e9021cad932238e9960953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Controlled release</topic><topic>Cytokines - metabolism</topic><topic>Delayed-Action Preparations - chemistry</topic><topic>Delayed-Action Preparations - pharmacokinetics</topic><topic>Delayed-Action Preparations - pharmacology</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - pharmacokinetics</topic><topic>Drug Carriers - pharmacology</topic><topic>Fallopia multiflora - chemistry</topic><topic>Female</topic><topic>Fluorouracil - chemistry</topic><topic>Fluorouracil - pharmacokinetics</topic><topic>Fluorouracil - pharmacology</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoprotection</topic><topic>Macrophages, Peritoneal - metabolism</topic><topic>Macrophages, Peritoneal - pathology</topic><topic>Mice</topic><topic>Neoplasms, Experimental - drug therapy</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Polygonum multiflorum polysaccharide</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - pharmacokinetics</topic><topic>Polysaccharides - pharmacology</topic><topic>Spleen - metabolism</topic><topic>Spleen - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qing</creatorcontrib><creatorcontrib>Xu, Yi</creatorcontrib><creatorcontrib>Lv, Junjiang</creatorcontrib><creatorcontrib>Cheng, Mengxia</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Cao, Kun</creatorcontrib><creatorcontrib>Zhang, Xiaofeng</creatorcontrib><creatorcontrib>Mou, Xiuni</creatorcontrib><creatorcontrib>Fan, Qi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qing</au><au>Xu, Yi</au><au>Lv, Junjiang</au><au>Cheng, Mengxia</au><au>Wu, Ying</au><au>Cao, Kun</au><au>Zhang, Xiaofeng</au><au>Mou, Xiuni</au><au>Fan, Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2018-09</date><risdate>2018</risdate><volume>116</volume><spage>1310</spage><epage>1316</epage><pages>1310-1316</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>WPMP-2 is an acid polysaccharide isolated from Polygonum multiflorum, which demonstrated excellent immunomodulatory activity. In order to reduce immunosuppression of 5-fluorouracil (5-Fu), WPMP-2 was utilized as a macromolecular carrier to conjugate the 5-Fu derivatives 5-fluorouracil-1-acetic acid (5-FUAC) through ester bond. The conjugate showed controlled drug release behaviour in vitro at 37°C in phosphate buffer (pH7.4), and only 5-FUAC was detected in the media. The cytotoxicity test in vitro showed that the conjugate exhibited different cytotoxicity to HepG-2 and HT-29 cells. In addition, immunization study in vivo illustrated that the conjugate displayed immunoprotective effect by mitigating inhibition and damage effects of 5-Fu on secretion of cytokines, proliferation of splenocytes, and phagocytosis of peritoneal macrophages. It was indicated that the conjugation of 5-Fu and WPMP-2 could be a potential double effective drug delivery system.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29432832</pmid><doi>10.1016/j.ijbiomac.2018.02.052</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-4698-0193</orcidid></addata></record> |
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| ispartof | International journal of biological macromolecules, 2018-09, Vol.116, p.1310-1316 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Controlled release Cytokines - metabolism Delayed-Action Preparations - chemistry Delayed-Action Preparations - pharmacokinetics Delayed-Action Preparations - pharmacology Drug Carriers - chemistry Drug Carriers - pharmacokinetics Drug Carriers - pharmacology Fallopia multiflora - chemistry Female Fluorouracil - chemistry Fluorouracil - pharmacokinetics Fluorouracil - pharmacology Hep G2 Cells Humans Hydrogen-Ion Concentration Immunoprotection Macrophages, Peritoneal - metabolism Macrophages, Peritoneal - pathology Mice Neoplasms, Experimental - drug therapy Neoplasms, Experimental - metabolism Neoplasms, Experimental - pathology Polygonum multiflorum polysaccharide Polysaccharides - chemistry Polysaccharides - pharmacokinetics Polysaccharides - pharmacology Spleen - metabolism Spleen - pathology |
| title | New utilization of Polygonum multiflorum polysaccharide as macromolecular carrier of 5-fluorouracil for controlled release and immunoprotection |
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