Differentially expressed genes in response to cyadox in swine liver analyzed by DDRT-PCR
Cyadox is a good antimicrobial growth-promoter of quinoxalines. However, the molecular mechanism of action remains unclear. A growth performance study and mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR) in combination with Northern dot-blot and reverse Northern d...
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| Veröffentlicht in: | Research in veterinary science 2018-06, Vol.118, p.72-78 |
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| creator | Yu, Rui Zhang, Yinfeng Lu, Qirong Cui, Luqing Wang, Yulian Wang, Xu Cheng, Guyue Liu, Zhenli Dai, Menghong Yuan, Zonghui |
| description | Cyadox is a good antimicrobial growth-promoter of quinoxalines. However, the molecular mechanism of action remains unclear. A growth performance study and mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR) in combination with Northern dot-blot and reverse Northern dot-blot analysis were conducted to determine the differentially expressed genes in liver tissues of piglets after treatment with cyadox. Transcription levels of the differentially expressed genes were quantificated by realtime RT-PCR in porcine primary hepotocytes. Cyadox could significantly promote body weight of piglets via feed with average daily gain (ADG) improved by 24.7% and 64.8% in 100 and 500mg/kg group, compared with control. A total of eight differentially expressed genes were found, of which the expression levels of five genes had positive correlation with cyadox dose. One gene expression had a negative correlation with cyadox dose and it was a new gene. The other two genes were up-regulated by cyadox, but the expression quantity was invariably when the cyadox doses were increased. Among the up-regulated genes, one was transcriptional regulating factor, two were growth-related factors, one was involved in immune defense and immune-regulation and three might be involved in the maintenance of normal development. In primary cultured pig hepatocytes, cyadox treatment evoked a significant time-dependent effect of eight genes expression. The results suggest, at the transcriptional level in vitro and in vivo, that growth factor and metabolism may be associated with cyadox growth-promoting activity, whereas immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.
•Cyadox could significantly promote body weight of piglets via feed.•At the transcriptional level, growth factor and metabolism may be associated with cyadox growth-promoting activity.•At the mRNA level, immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets. |
| doi_str_mv | 10.1016/j.rvsc.2018.01.014 |
| format | Article |
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•Cyadox could significantly promote body weight of piglets via feed.•At the transcriptional level, growth factor and metabolism may be associated with cyadox growth-promoting activity.•At the mRNA level, immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2018.01.014</identifier><identifier>PMID: 29421487</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibiotics ; Antimicrobial agents ; Blotting, Northern - methods ; Blotting, Northern - veterinary ; Body weight ; Cyadox ; DDRT-PCR ; Differential display ; Differentially expressed genes ; Drug dosages ; Epidermal growth factor ; Experiments ; Feeds ; Gene expression ; Gene Expression Profiling ; Genes ; Growth hormones ; Hepatocytes ; Hogs ; Immune system ; Insulin ; Insulin-like growth factors ; Laboratory animals ; Liver ; Liver - metabolism ; Livestock ; Metabolism ; Molecular chains ; Nutrition research ; Piglets ; Polymerase chain reaction ; Prophylaxis ; Quinoxalines ; Quinoxalines - pharmacology ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; Reverse transcription ; Suidae ; Sus scrofa ; Swine ; Time dependence ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2018-06, Vol.118, p.72-78</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jun 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-7e510731b41125803911cf0c4aeb9cfcdcd0a6946ef6b45c9700a94385a075b83</citedby><cites>FETCH-LOGICAL-c384t-7e510731b41125803911cf0c4aeb9cfcdcd0a6946ef6b45c9700a94385a075b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2018.01.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29421487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Rui</creatorcontrib><creatorcontrib>Zhang, Yinfeng</creatorcontrib><creatorcontrib>Lu, Qirong</creatorcontrib><creatorcontrib>Cui, Luqing</creatorcontrib><creatorcontrib>Wang, Yulian</creatorcontrib><creatorcontrib>Wang, Xu</creatorcontrib><creatorcontrib>Cheng, Guyue</creatorcontrib><creatorcontrib>Liu, Zhenli</creatorcontrib><creatorcontrib>Dai, Menghong</creatorcontrib><creatorcontrib>Yuan, Zonghui</creatorcontrib><title>Differentially expressed genes in response to cyadox in swine liver analyzed by DDRT-PCR</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Cyadox is a good antimicrobial growth-promoter of quinoxalines. However, the molecular mechanism of action remains unclear. A growth performance study and mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR) in combination with Northern dot-blot and reverse Northern dot-blot analysis were conducted to determine the differentially expressed genes in liver tissues of piglets after treatment with cyadox. Transcription levels of the differentially expressed genes were quantificated by realtime RT-PCR in porcine primary hepotocytes. Cyadox could significantly promote body weight of piglets via feed with average daily gain (ADG) improved by 24.7% and 64.8% in 100 and 500mg/kg group, compared with control. A total of eight differentially expressed genes were found, of which the expression levels of five genes had positive correlation with cyadox dose. One gene expression had a negative correlation with cyadox dose and it was a new gene. The other two genes were up-regulated by cyadox, but the expression quantity was invariably when the cyadox doses were increased. Among the up-regulated genes, one was transcriptional regulating factor, two were growth-related factors, one was involved in immune defense and immune-regulation and three might be involved in the maintenance of normal development. In primary cultured pig hepatocytes, cyadox treatment evoked a significant time-dependent effect of eight genes expression. The results suggest, at the transcriptional level in vitro and in vivo, that growth factor and metabolism may be associated with cyadox growth-promoting activity, whereas immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.
•Cyadox could significantly promote body weight of piglets via feed.•At the transcriptional level, growth factor and metabolism may be associated with cyadox growth-promoting activity.•At the mRNA level, immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.</description><subject>Animals</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Blotting, Northern - methods</subject><subject>Blotting, Northern - veterinary</subject><subject>Body weight</subject><subject>Cyadox</subject><subject>DDRT-PCR</subject><subject>Differential display</subject><subject>Differentially expressed genes</subject><subject>Drug dosages</subject><subject>Epidermal growth factor</subject><subject>Experiments</subject><subject>Feeds</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Growth hormones</subject><subject>Hepatocytes</subject><subject>Hogs</subject><subject>Immune system</subject><subject>Insulin</subject><subject>Insulin-like growth factors</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Livestock</subject><subject>Metabolism</subject><subject>Molecular chains</subject><subject>Nutrition research</subject><subject>Piglets</subject><subject>Polymerase chain reaction</subject><subject>Prophylaxis</subject><subject>Quinoxalines</subject><subject>Quinoxalines - pharmacology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>Reverse transcription</subject><subject>Suidae</subject><subject>Sus scrofa</subject><subject>Swine</subject><subject>Time dependence</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSTYffyCHIuilF29nZNmWIZew23xAoCUkkJuQ5XHQ4rU3kncb99dXZtMccigMCA3P-zI8jJ0jzBEw_76a-12wcwGo5oBx5AGbYZaKROQ5fmIzgFQmmVDqiB2HsAIAiVgcsiNRSoFSFTP2tHRNQ566wZm2HTm9bjyFQDV_po4Cdx2P_03fBeJDz-1o6v512obfriPeuh15bjrTjn9iphr5cnn_kPxa3J-yz41pA529vSfs8erHw-Imuft5fbu4vEtsquSQFJQhFClW8TKRKUhLRNuAlYaq0ja2tjWYvJQ5NXklM1sWAKaUqcoMFFml0hP2bd-78f3LlsKg1y5YalvTUb8NWgAg5KByiOjXD-iq3_p4-0TJFIUUZRkpsaes70Pw1OiNd2vjR42gJ-96pSfvevKuAePIGPryVr2t1lS_R_6JjsDFHqDoYufI62AddZZq58kOuu7d__r_AkoBknE</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Yu, Rui</creator><creator>Zhang, Yinfeng</creator><creator>Lu, Qirong</creator><creator>Cui, Luqing</creator><creator>Wang, Yulian</creator><creator>Wang, Xu</creator><creator>Cheng, Guyue</creator><creator>Liu, Zhenli</creator><creator>Dai, Menghong</creator><creator>Yuan, Zonghui</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201806</creationdate><title>Differentially expressed genes in response to cyadox in swine liver analyzed by DDRT-PCR</title><author>Yu, Rui ; 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However, the molecular mechanism of action remains unclear. A growth performance study and mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR) in combination with Northern dot-blot and reverse Northern dot-blot analysis were conducted to determine the differentially expressed genes in liver tissues of piglets after treatment with cyadox. Transcription levels of the differentially expressed genes were quantificated by realtime RT-PCR in porcine primary hepotocytes. Cyadox could significantly promote body weight of piglets via feed with average daily gain (ADG) improved by 24.7% and 64.8% in 100 and 500mg/kg group, compared with control. A total of eight differentially expressed genes were found, of which the expression levels of five genes had positive correlation with cyadox dose. One gene expression had a negative correlation with cyadox dose and it was a new gene. The other two genes were up-regulated by cyadox, but the expression quantity was invariably when the cyadox doses were increased. Among the up-regulated genes, one was transcriptional regulating factor, two were growth-related factors, one was involved in immune defense and immune-regulation and three might be involved in the maintenance of normal development. In primary cultured pig hepatocytes, cyadox treatment evoked a significant time-dependent effect of eight genes expression. The results suggest, at the transcriptional level in vitro and in vivo, that growth factor and metabolism may be associated with cyadox growth-promoting activity, whereas immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.
•Cyadox could significantly promote body weight of piglets via feed.•At the transcriptional level, growth factor and metabolism may be associated with cyadox growth-promoting activity.•At the mRNA level, immune defense and immune-regulation could play major roles in prophylaxis of cyadox in piglets.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29421487</pmid><doi>10.1016/j.rvsc.2018.01.014</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Antibiotics Antimicrobial agents Blotting, Northern - methods Blotting, Northern - veterinary Body weight Cyadox DDRT-PCR Differential display Differentially expressed genes Drug dosages Epidermal growth factor Experiments Feeds Gene expression Gene Expression Profiling Genes Growth hormones Hepatocytes Hogs Immune system Insulin Insulin-like growth factors Laboratory animals Liver Liver - metabolism Livestock Metabolism Molecular chains Nutrition research Piglets Polymerase chain reaction Prophylaxis Quinoxalines Quinoxalines - pharmacology Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - veterinary Reverse transcription Suidae Sus scrofa Swine Time dependence Veterinary medicine |
| title | Differentially expressed genes in response to cyadox in swine liver analyzed by DDRT-PCR |
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