Bio-affinity mediated immobilization of lipase onto magnetic cellulose nanospheres for high yield biodiesel in one time addition of methanol

•GSlip-CBD-MCNS was used to obtaining high yield of biodiesel from palm oil.•GSlip-CBD-MCNS showed high tolerance to methanol and temperature.•98% of biodiesel was obtained in 4 h in one step addition of methanol.•The biocatalyst was highly reusable with 94% of biodiesel until 8th cycle.•Preparation...

Ausführliche Beschreibung