Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR

Phytases are used to improve phosphorus nutrition of food animals and reduce their phosphorus excretion to the environment. Due to favorable properties, Escherichia coli AppA2 phytase is of particular interest for biotechnological applications. Directed evolution was applied in the present study to...

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Veröffentlicht in:	Applied microbiology and biotechnology 2008-05, Vol.79 (1), p.69-75
Hauptverfasser:	Kim, Moon-Soo, Lei, Xin Gen
Format:	Artikel
Sprache:	eng
Schlagworte:	6-Phytase - genetics 6-Phytase - metabolism Acid Phosphatase - genetics Acid Phosphatase - metabolism Animal sciences Bacteria Biological and medical sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Calorimetry, Differential Scanning Catalysis Directed Molecular Evolution E coli Efficiency Enzyme Enzyme Stability Enzymes Error-prone PCR Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Hydrogen Bonding Hydrogen bonds Hydrogen-Ion Concentration Inactivation Kinetics Life Sciences Melting temperature Methods. Procedures. Technologies Microbial Genetics and Genomics Microbiology Models, Molecular Multienzyme Complexes - genetics Multienzyme Complexes - metabolism Mutagenesis Phosphatase Phosphorus Phytase Pichia pastoris Polymerase Chain Reaction Protein Engineering Protein Structure, Secondary Proteins Saccharomyces cerevisiae Studies Temperature thermal stability Transition Temperature Yeast Zoology
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