Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco
Aquaporin (AQP) proteins have been shown to transport water and other small molecules through biological membranes, which is crucial for plants to combat salt stress. However, the precise role of AQP genes in salt stress response is not completely understood in plants. In this study, a PIP1 subgroup...
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| Veröffentlicht in: | Plant and cell physiology 2012-12, Vol.53 (12), p.2127-2141 |
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| creator | Hu, Wei Yuan, Qianqian Wang, Yan Cai, Rui Deng, Xiaomin Wang, Jie Zhou, Shiyi Chen, Mingjie Chen, Lihong Huang, Chao Ma, Zhanbing Yang, Guangxiao He, Guangyuan |
| description | Aquaporin (AQP) proteins have been shown to transport water and other small molecules through biological membranes, which is crucial for plants to combat salt stress. However, the precise role of AQP genes in salt stress response is not completely understood in plants. In this study, a PIP1 subgroup AQP gene, designated TaAQP8, was cloned and characterized from wheat. Transient expression of TaAQP8-green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes. TaAQP8 transcript was induced by NaCl, ethylene and H(2)O(2). Further investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H(2)O(2) signaling, with ethylene causing a positive effect and H(2)O(2) acting as a negative factor. Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K(+)/Na(+) ratio and Ca(2+) content, but reduced H(2)O(2) accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress. Further investigation showed that whole seedlings from transgenic lines displayed higher SOD, CAT and POD activities, increased NtSOD and NtCAT transcript levels, and decreased H(2)O(2) accumulation and membrane injury under salt stress. Taken together, our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K(+)/Na(+) ratio and Ca(2+) content, but also by reducing H(2)O(2) accumulation and membrane damage by enhancing the antioxidant system. |
| doi_str_mv | 10.1093/pcp/pcs154 |
| format | Article |
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However, the precise role of AQP genes in salt stress response is not completely understood in plants. In this study, a PIP1 subgroup AQP gene, designated TaAQP8, was cloned and characterized from wheat. Transient expression of TaAQP8-green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes. TaAQP8 transcript was induced by NaCl, ethylene and H(2)O(2). Further investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H(2)O(2) signaling, with ethylene causing a positive effect and H(2)O(2) acting as a negative factor. Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K(+)/Na(+) ratio and Ca(2+) content, but reduced H(2)O(2) accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress. Further investigation showed that whole seedlings from transgenic lines displayed higher SOD, CAT and POD activities, increased NtSOD and NtCAT transcript levels, and decreased H(2)O(2) accumulation and membrane injury under salt stress. Taken together, our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K(+)/Na(+) ratio and Ca(2+) content, but also by reducing H(2)O(2) accumulation and membrane damage by enhancing the antioxidant system.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcs154</identifier><identifier>PMID: 23161856</identifier><language>eng</language><publisher>Japan</publisher><subject>Animals ; aquaporins ; Aquaporins - genetics ; Aquaporins - metabolism ; Base Sequence ; calcium ; catalase ; Catalase - metabolism ; Cations - metabolism ; Cell Membrane - metabolism ; ethylene ; Ethylenes - pharmacology ; fluorescent proteins ; Gene Expression Regulation, Plant ; gene overexpression ; genes ; hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; Molecular Sequence Data ; Nicotiana - drug effects ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana - physiology ; Onions - genetics ; Onions - metabolism ; Oocytes ; peroxidase ; Peroxidase - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - drug effects ; Plant Roots - genetics ; Plant Roots - metabolism ; Plant Roots - physiology ; Plants, Genetically Modified ; plasma membrane ; potassium ; Recombinant Fusion Proteins ; root growth ; roots ; salt stress ; Salt Tolerance - genetics ; seedlings ; Seedlings - drug effects ; Seedlings - genetics ; Seedlings - metabolism ; Seedlings - physiology ; Sequence Analysis, DNA ; Signal Transduction ; sodium ; sodium chloride ; Sodium Chloride - pharmacology ; stress response ; stress tolerance ; superoxide dismutase ; Superoxide Dismutase - metabolism ; tobacco ; transgenic plants ; Triticum - drug effects ; Triticum - genetics ; Triticum - metabolism ; Triticum - physiology ; Triticum aestivum ; wheat ; Xenopus laevis ; Xenopus laevis - genetics ; Xenopus laevis - metabolism</subject><ispartof>Plant and cell physiology, 2012-12, Vol.53 (12), p.2127-2141</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-d175341fb66c841bdf1017f808fc55198beb5b572f2a72f9225377496cffc1523</citedby><cites>FETCH-LOGICAL-c416t-d175341fb66c841bdf1017f808fc55198beb5b572f2a72f9225377496cffc1523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23161856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Wei</creatorcontrib><creatorcontrib>Yuan, Qianqian</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Cai, Rui</creatorcontrib><creatorcontrib>Deng, Xiaomin</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Zhou, Shiyi</creatorcontrib><creatorcontrib>Chen, Mingjie</creatorcontrib><creatorcontrib>Chen, Lihong</creatorcontrib><creatorcontrib>Huang, Chao</creatorcontrib><creatorcontrib>Ma, Zhanbing</creatorcontrib><creatorcontrib>Yang, Guangxiao</creatorcontrib><creatorcontrib>He, Guangyuan</creatorcontrib><title>Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Aquaporin (AQP) proteins have been shown to transport water and other small molecules through biological membranes, which is crucial for plants to combat salt stress. However, the precise role of AQP genes in salt stress response is not completely understood in plants. In this study, a PIP1 subgroup AQP gene, designated TaAQP8, was cloned and characterized from wheat. Transient expression of TaAQP8-green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes. TaAQP8 transcript was induced by NaCl, ethylene and H(2)O(2). Further investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H(2)O(2) signaling, with ethylene causing a positive effect and H(2)O(2) acting as a negative factor. Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K(+)/Na(+) ratio and Ca(2+) content, but reduced H(2)O(2) accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress. Further investigation showed that whole seedlings from transgenic lines displayed higher SOD, CAT and POD activities, increased NtSOD and NtCAT transcript levels, and decreased H(2)O(2) accumulation and membrane injury under salt stress. Taken together, our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K(+)/Na(+) ratio and Ca(2+) content, but also by reducing H(2)O(2) accumulation and membrane damage by enhancing the antioxidant system.</description><subject>Animals</subject><subject>aquaporins</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Base Sequence</subject><subject>calcium</subject><subject>catalase</subject><subject>Catalase - metabolism</subject><subject>Cations - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>ethylene</subject><subject>Ethylenes - pharmacology</subject><subject>fluorescent proteins</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene overexpression</subject><subject>genes</subject><subject>hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - drug effects</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - physiology</subject><subject>Onions - genetics</subject><subject>Onions - metabolism</subject><subject>Oocytes</subject><subject>peroxidase</subject><subject>Peroxidase - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - metabolism</subject><subject>Plant Roots - physiology</subject><subject>Plants, Genetically Modified</subject><subject>plasma membrane</subject><subject>potassium</subject><subject>Recombinant Fusion Proteins</subject><subject>root growth</subject><subject>roots</subject><subject>salt stress</subject><subject>Salt Tolerance - genetics</subject><subject>seedlings</subject><subject>Seedlings - drug effects</subject><subject>Seedlings - genetics</subject><subject>Seedlings - metabolism</subject><subject>Seedlings - physiology</subject><subject>Sequence Analysis, DNA</subject><subject>Signal Transduction</subject><subject>sodium</subject><subject>sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>stress response</subject><subject>stress tolerance</subject><subject>superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>tobacco</subject><subject>transgenic plants</subject><subject>Triticum - drug effects</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum - physiology</subject><subject>Triticum aestivum</subject><subject>wheat</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis - genetics</subject><subject>Xenopus laevis - metabolism</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctOwzAQRS0EoqWw4QOQlwg14LHjJF6iipdUqSCVdeS4YxqUxqmd8vh7XLWwZTEPjc69i7mEnAO7BqbETWe6WAFkekCGkOaQKCbFIRkyJnjC8gIG5CSEd8biLtgxGXABGRQyGxI7-0CPX53HEGrXUmeppp9L1D3V643unK9b-oYtjulc3748F2OK7VK3BgMNuulp6LdS2rsG_fZMI9_HLURRbeK90sa4U3JkdRPwbD9H5PX-bj55TKazh6fJ7TQxKWR9soBcihRslWWmSKFaWGCQ24IV1kgJqqiwkpXMueU6NsW5FHmeqsxYa0ByMSKXO9_Ou_UGQ1-u6mCwaXSLbhNKHn-QglCF-BcFATJjUnEV0asdarwLwaMtO1-vtP8ugZXbCMoYQbmLIMIXe99NtcLFH_r7c_EDNYCCIg</recordid><startdate>201212</startdate><enddate>201212</enddate><creator>Hu, Wei</creator><creator>Yuan, Qianqian</creator><creator>Wang, Yan</creator><creator>Cai, Rui</creator><creator>Deng, Xiaomin</creator><creator>Wang, Jie</creator><creator>Zhou, Shiyi</creator><creator>Chen, Mingjie</creator><creator>Chen, Lihong</creator><creator>Huang, Chao</creator><creator>Ma, Zhanbing</creator><creator>Yang, Guangxiao</creator><creator>He, Guangyuan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>201212</creationdate><title>Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco</title><author>Hu, Wei ; Yuan, Qianqian ; Wang, Yan ; Cai, Rui ; Deng, Xiaomin ; Wang, Jie ; Zhou, Shiyi ; Chen, Mingjie ; Chen, Lihong ; Huang, Chao ; Ma, Zhanbing ; Yang, Guangxiao ; He, Guangyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-d175341fb66c841bdf1017f808fc55198beb5b572f2a72f9225377496cffc1523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>aquaporins</topic><topic>Aquaporins - genetics</topic><topic>Aquaporins - metabolism</topic><topic>Base Sequence</topic><topic>calcium</topic><topic>catalase</topic><topic>Catalase - metabolism</topic><topic>Cations - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>ethylene</topic><topic>Ethylenes - pharmacology</topic><topic>fluorescent proteins</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene overexpression</topic><topic>genes</topic><topic>hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - drug effects</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana - physiology</topic><topic>Onions - genetics</topic><topic>Onions - metabolism</topic><topic>Oocytes</topic><topic>peroxidase</topic><topic>Peroxidase - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots - drug effects</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - metabolism</topic><topic>Plant Roots - physiology</topic><topic>Plants, Genetically Modified</topic><topic>plasma membrane</topic><topic>potassium</topic><topic>Recombinant Fusion Proteins</topic><topic>root growth</topic><topic>roots</topic><topic>salt stress</topic><topic>Salt Tolerance - genetics</topic><topic>seedlings</topic><topic>Seedlings - drug effects</topic><topic>Seedlings - genetics</topic><topic>Seedlings - metabolism</topic><topic>Seedlings - physiology</topic><topic>Sequence Analysis, DNA</topic><topic>Signal Transduction</topic><topic>sodium</topic><topic>sodium chloride</topic><topic>Sodium Chloride - pharmacology</topic><topic>stress response</topic><topic>stress tolerance</topic><topic>superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>tobacco</topic><topic>transgenic plants</topic><topic>Triticum - drug effects</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum - physiology</topic><topic>Triticum aestivum</topic><topic>wheat</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - genetics</topic><topic>Xenopus laevis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Wei</creatorcontrib><creatorcontrib>Yuan, Qianqian</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Cai, Rui</creatorcontrib><creatorcontrib>Deng, Xiaomin</creatorcontrib><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Zhou, Shiyi</creatorcontrib><creatorcontrib>Chen, Mingjie</creatorcontrib><creatorcontrib>Chen, Lihong</creatorcontrib><creatorcontrib>Huang, Chao</creatorcontrib><creatorcontrib>Ma, Zhanbing</creatorcontrib><creatorcontrib>Yang, Guangxiao</creatorcontrib><creatorcontrib>He, Guangyuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Wei</au><au>Yuan, Qianqian</au><au>Wang, Yan</au><au>Cai, Rui</au><au>Deng, Xiaomin</au><au>Wang, Jie</au><au>Zhou, Shiyi</au><au>Chen, Mingjie</au><au>Chen, Lihong</au><au>Huang, Chao</au><au>Ma, Zhanbing</au><au>Yang, Guangxiao</au><au>He, Guangyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2012-12</date><risdate>2012</risdate><volume>53</volume><issue>12</issue><spage>2127</spage><epage>2141</epage><pages>2127-2141</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Aquaporin (AQP) proteins have been shown to transport water and other small molecules through biological membranes, which is crucial for plants to combat salt stress. However, the precise role of AQP genes in salt stress response is not completely understood in plants. In this study, a PIP1 subgroup AQP gene, designated TaAQP8, was cloned and characterized from wheat. Transient expression of TaAQP8-green fluorescent protein (GFP) fusion protein revealed its localization in the plasma membrane. TaAQP8 exhibited water channel activity in Xenopus laevis oocytes. TaAQP8 transcript was induced by NaCl, ethylene and H(2)O(2). Further investigation showed that up-regulation of TaAQP8 under salt stress involves ethylene and H(2)O(2) signaling, with ethylene causing a positive effect and H(2)O(2) acting as a negative factor. Overexpression of TaAQP8 in tobacco increased root elongation compared with controls under salt stress. The roots of transgenic plants also retained a high K(+)/Na(+) ratio and Ca(2+) content, but reduced H(2)O(2) accumulation by an enhancement of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under salt stress. Further investigation showed that whole seedlings from transgenic lines displayed higher SOD, CAT and POD activities, increased NtSOD and NtCAT transcript levels, and decreased H(2)O(2) accumulation and membrane injury under salt stress. Taken together, our results demonstrate that TaAQP8 confers salt stress tolerance not only by retaining high a K(+)/Na(+) ratio and Ca(2+) content, but also by reducing H(2)O(2) accumulation and membrane damage by enhancing the antioxidant system.</abstract><cop>Japan</cop><pmid>23161856</pmid><doi>10.1093/pcp/pcs154</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals aquaporins Aquaporins - genetics Aquaporins - metabolism Base Sequence calcium catalase Catalase - metabolism Cations - metabolism Cell Membrane - metabolism ethylene Ethylenes - pharmacology fluorescent proteins Gene Expression Regulation, Plant gene overexpression genes hydrogen peroxide Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology Molecular Sequence Data Nicotiana - drug effects Nicotiana - genetics Nicotiana - metabolism Nicotiana - physiology Onions - genetics Onions - metabolism Oocytes peroxidase Peroxidase - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - drug effects Plant Roots - genetics Plant Roots - metabolism Plant Roots - physiology Plants, Genetically Modified plasma membrane potassium Recombinant Fusion Proteins root growth roots salt stress Salt Tolerance - genetics seedlings Seedlings - drug effects Seedlings - genetics Seedlings - metabolism Seedlings - physiology Sequence Analysis, DNA Signal Transduction sodium sodium chloride Sodium Chloride - pharmacology stress response stress tolerance superoxide dismutase Superoxide Dismutase - metabolism tobacco transgenic plants Triticum - drug effects Triticum - genetics Triticum - metabolism Triticum - physiology Triticum aestivum wheat Xenopus laevis Xenopus laevis - genetics Xenopus laevis - metabolism |
| title | Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco |
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