TAT Peptide-Functionalized Gold Nanostars: Enhanced Intracellular Delivery and Efficient NIR Photothermal Therapy Using Ultralow Irradiance
Gold nanoparticles have great potential in plasmonic photothermal therapy (photothermolysis), but their intracellular delivery and photothermolysis efficiency have yet to be optimized. We show that TAT-peptide-functionalized gold nanostars (NS) enter cells significantly more than bare or PEGylated N...
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| Veröffentlicht in: | Journal of the American Chemical Society 2012-07, Vol.134 (28), p.11358-11361 |
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| container_end_page | 11361 |
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| container_issue | 28 |
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| container_title | Journal of the American Chemical Society |
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| creator | Yuan, Hsiangkuo Fales, Andrew M Vo-Dinh, Tuan |
| description | Gold nanoparticles have great potential in plasmonic photothermal therapy (photothermolysis), but their intracellular delivery and photothermolysis efficiency have yet to be optimized. We show that TAT-peptide-functionalized gold nanostars (NS) enter cells significantly more than bare or PEGylated NS. The cellular uptake mechanism involves actin-driven lipid raft-mediated macropinocytosis, where particles primarily accumulate in macropinosomes but may also leak out into the cytoplasm. After 4-h incubation of TAT-NS on BT549 breast cancer cells, photothermolysis was accomplished using 850 nm pulsed laser under 0.2 W/cm2 irradiation, below the maximal permissible exposure of skin. These results demonstrate the enhanced intracellular delivery and efficient photothermolysis of TAT-NS, promising agents in cancer therapy. |
| doi_str_mv | 10.1021/ja304180y |
| format | Article |
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Am. Chem. Soc</addtitle><description>Gold nanoparticles have great potential in plasmonic photothermal therapy (photothermolysis), but their intracellular delivery and photothermolysis efficiency have yet to be optimized. We show that TAT-peptide-functionalized gold nanostars (NS) enter cells significantly more than bare or PEGylated NS. The cellular uptake mechanism involves actin-driven lipid raft-mediated macropinocytosis, where particles primarily accumulate in macropinosomes but may also leak out into the cytoplasm. After 4-h incubation of TAT-NS on BT549 breast cancer cells, photothermolysis was accomplished using 850 nm pulsed laser under 0.2 W/cm2 irradiation, below the maximal permissible exposure of skin. These results demonstrate the enhanced intracellular delivery and efficient photothermolysis of TAT-NS, promising agents in cancer therapy.</description><subject>breast neoplasms</subject><subject>Cell Line, Tumor</subject><subject>cytoplasm</subject><subject>Female</subject><subject>Gene Products, tat - chemistry</subject><subject>gold</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Hyperthermia, Induced</subject><subject>irradiation</subject><subject>Light</subject><subject>light intensity</subject><subject>lipids</subject><subject>Metal Nanoparticles</subject><subject>Microscopy, Electron, Transmission</subject><subject>nanogold</subject><subject>neoplasm cells</subject><subject>Phototherapy</subject><subject>therapeutics</subject><issn>0002-7863</issn><issn>1520-5126</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVpaJy0h_6BokshOWyqr92VewuJkxpCGop9XibSbC0jS6602-L8hf7pyjjNqdDTyzAPD8y8hLzn7IIzwT-tQTLFNdu9IhNeC1bVXDSvyYQxJqpWN_KYnOS8LqMSmr8hx0K0UjVMT8jvxeWCPuB2cBarmzGYwcUA3j2hpbfRW3oPIeYBUv5MZ2EFwZTFPAwJDHo_ekj0Gr37iWlHIVg663tnHIaB3s-_0YdVHOKwwrQBTxclYbujy-zCd7r0xeHjLzpPCazbi9-Sox58xnfPeUqWN7PF1Zfq7uvt_OryrgKlpkNlpdTW6FoxACa0amvV8LqWhtlHbqUGbLERDUjoDUAvtDFTA7xniKitkvKUnB282xR_jJiHbuPy_hwIGMfcifIoKfhUNf9Fy_tbppjWe_T8gJoUc07Yd9vkNpB2BdpzvHupqbAfnrXj4wbtC_m3lwJ8PABgcreOYyqd5H-I_gDC4prK</recordid><startdate>20120718</startdate><enddate>20120718</enddate><creator>Yuan, Hsiangkuo</creator><creator>Fales, Andrew M</creator><creator>Vo-Dinh, Tuan</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20120718</creationdate><title>TAT Peptide-Functionalized Gold Nanostars: Enhanced Intracellular Delivery and Efficient NIR Photothermal Therapy Using Ultralow Irradiance</title><author>Yuan, Hsiangkuo ; Fales, Andrew M ; Vo-Dinh, Tuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a449t-d338dc8540aa028475461553c0db1d38ae7e626a3afcaaf28cc9ca1f0eee8d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>breast neoplasms</topic><topic>Cell Line, Tumor</topic><topic>cytoplasm</topic><topic>Female</topic><topic>Gene Products, tat - chemistry</topic><topic>gold</topic><topic>Gold - chemistry</topic><topic>Humans</topic><topic>Hyperthermia, Induced</topic><topic>irradiation</topic><topic>Light</topic><topic>light intensity</topic><topic>lipids</topic><topic>Metal Nanoparticles</topic><topic>Microscopy, Electron, Transmission</topic><topic>nanogold</topic><topic>neoplasm cells</topic><topic>Phototherapy</topic><topic>therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Hsiangkuo</creatorcontrib><creatorcontrib>Fales, Andrew M</creatorcontrib><creatorcontrib>Vo-Dinh, Tuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Hsiangkuo</au><au>Fales, Andrew M</au><au>Vo-Dinh, Tuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TAT Peptide-Functionalized Gold Nanostars: Enhanced Intracellular Delivery and Efficient NIR Photothermal Therapy Using Ultralow Irradiance</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2012-07-18</date><risdate>2012</risdate><volume>134</volume><issue>28</issue><spage>11358</spage><epage>11361</epage><pages>11358-11361</pages><issn>0002-7863</issn><issn>1520-5126</issn><eissn>1520-5126</eissn><abstract>Gold nanoparticles have great potential in plasmonic photothermal therapy (photothermolysis), but their intracellular delivery and photothermolysis efficiency have yet to be optimized. We show that TAT-peptide-functionalized gold nanostars (NS) enter cells significantly more than bare or PEGylated NS. The cellular uptake mechanism involves actin-driven lipid raft-mediated macropinocytosis, where particles primarily accumulate in macropinosomes but may also leak out into the cytoplasm. After 4-h incubation of TAT-NS on BT549 breast cancer cells, photothermolysis was accomplished using 850 nm pulsed laser under 0.2 W/cm2 irradiation, below the maximal permissible exposure of skin. These results demonstrate the enhanced intracellular delivery and efficient photothermolysis of TAT-NS, promising agents in cancer therapy.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>22734608</pmid><doi>10.1021/ja304180y</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | breast neoplasms Cell Line, Tumor cytoplasm Female Gene Products, tat - chemistry gold Gold - chemistry Humans Hyperthermia, Induced irradiation Light light intensity lipids Metal Nanoparticles Microscopy, Electron, Transmission nanogold neoplasm cells Phototherapy therapeutics |
| title | TAT Peptide-Functionalized Gold Nanostars: Enhanced Intracellular Delivery and Efficient NIR Photothermal Therapy Using Ultralow Irradiance |
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