Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay
Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communi...
Gespeichert in:
| Veröffentlicht in: | Journal of microbiological methods 2016-03, Vol.122, p.27-32 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 32 |
|---|---|
| container_issue | |
| container_start_page | 27 |
| container_title | Journal of microbiological methods |
| container_volume | 122 |
| creator | Yeh, Hung-Yueh Serrano, Katherine Vargas Acosta, Aimee Silvestry Buhr, Richard J. |
| description | Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
•Production of Salmonella flagellar protein FlgK•Immunization of chickens with the FlgK protein•Use of FlgK antibodies from chicken sera to develop automated capillary immunoassay |
| doi_str_mv | 10.1016/j.mimet.2016.01.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2000319346</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0167701216300070</els_id><sourcerecordid>1767069702</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-98e3395cdab44868def035d7af9d15e1db1654a1cbe49f5a8f0b382debf2cd753</originalsourceid><addsrcrecordid>eNqNkc1u1TAQhS0EoreFJ0BCXrJogh0nsbNggaqWIiq1ErC2_DMpvsT2JXYq3ffpg-JwS8UKWNkjf3POeA5CryipKaH9223tnYdcN6WoCa0J4U_QhgreVIJ1w1O0KQ-84oQ2R-g4pS0htGOteI6Omp6LljO-Qfc3c7SLyS4GHEc8g4leu6BCxp_V5GOAaVJ4nNTtepnxbo4ZXDjFF9Ptp1OsgsUuJ7wkSNgFbCHDo1gRcdUfKmuto3UH1Hxz5juEhPUeqyVHrzJYbNTOrUZ77LxfQlQpqf0L9GxUU4KXD-cJ-npx_uXssrq6_vDx7P1VZVpOcjUIYGzojFW6bUUvLIyEdZarcbC0A2o17btWUaOhHcZOiZFoJhoLemyM5R07QW8OuuWXPxZIWXqXzDp7gLgk2RBCGB1Y2_8TpbwXnaCipf-DctIPnDQFZQfUzDGlGUa5m50v25CUyDV0uZW_Qpdr6JJQWUIvXa8fDBbtwT72_E65AO8OAJTl3TmYZTIOggHrSuBZ2uj-avATXqfCRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1767069702</pqid></control><display><type>article</type><title>Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yeh, Hung-Yueh ; Serrano, Katherine Vargas ; Acosta, Aimee Silvestry ; Buhr, Richard J.</creator><creatorcontrib>Yeh, Hung-Yueh ; Serrano, Katherine Vargas ; Acosta, Aimee Silvestry ; Buhr, Richard J.</creatorcontrib><description>Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
•Production of Salmonella flagellar protein FlgK•Immunization of chickens with the FlgK protein•Use of FlgK antibodies from chicken sera to develop automated capillary immunoassay</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2016.01.007</identifier><identifier>PMID: 26784737</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; antibodies ; Antibodies, Bacterial - blood ; Automation ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacteriological Techniques - instrumentation ; Bacteriological Techniques - methods ; Base Sequence ; chickens ; Chickens - blood ; Chickens - immunology ; Flagellar proteins ; FlgK ; Immunoassay - methods ; immunoassays ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Salmonella ; Salmonella enterica ; Salmonella enterica - genetics ; Salmonella enterica - immunology ; Salmonella Infections, Animal - diagnosis ; Salmonella Infections, Animal - immunology ; Salmonella Infections, Animal - microbiology ; Simple Western ; Zoonoses ; Zoonoses - diagnosis ; Zoonoses - immunology ; Zoonoses - microbiology</subject><ispartof>Journal of microbiological methods, 2016-03, Vol.122, p.27-32</ispartof><rights>2015</rights><rights>Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-98e3395cdab44868def035d7af9d15e1db1654a1cbe49f5a8f0b382debf2cd753</citedby><cites>FETCH-LOGICAL-c470t-98e3395cdab44868def035d7af9d15e1db1654a1cbe49f5a8f0b382debf2cd753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167701216300070$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26784737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yeh, Hung-Yueh</creatorcontrib><creatorcontrib>Serrano, Katherine Vargas</creatorcontrib><creatorcontrib>Acosta, Aimee Silvestry</creatorcontrib><creatorcontrib>Buhr, Richard J.</creatorcontrib><title>Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
•Production of Salmonella flagellar protein FlgK•Immunization of chickens with the FlgK protein•Use of FlgK antibodies from chicken sera to develop automated capillary immunoassay</description><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Automation</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriological Techniques - instrumentation</subject><subject>Bacteriological Techniques - methods</subject><subject>Base Sequence</subject><subject>chickens</subject><subject>Chickens - blood</subject><subject>Chickens - immunology</subject><subject>Flagellar proteins</subject><subject>FlgK</subject><subject>Immunoassay - methods</subject><subject>immunoassays</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella enterica - genetics</subject><subject>Salmonella enterica - immunology</subject><subject>Salmonella Infections, Animal - diagnosis</subject><subject>Salmonella Infections, Animal - immunology</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Simple Western</subject><subject>Zoonoses</subject><subject>Zoonoses - diagnosis</subject><subject>Zoonoses - immunology</subject><subject>Zoonoses - microbiology</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1TAQhS0EoreFJ0BCXrJogh0nsbNggaqWIiq1ErC2_DMpvsT2JXYq3ffpg-JwS8UKWNkjf3POeA5CryipKaH9223tnYdcN6WoCa0J4U_QhgreVIJ1w1O0KQ-84oQ2R-g4pS0htGOteI6Omp6LljO-Qfc3c7SLyS4GHEc8g4leu6BCxp_V5GOAaVJ4nNTtepnxbo4ZXDjFF9Ptp1OsgsUuJ7wkSNgFbCHDo1gRcdUfKmuto3UH1Hxz5juEhPUeqyVHrzJYbNTOrUZ77LxfQlQpqf0L9GxUU4KXD-cJ-npx_uXssrq6_vDx7P1VZVpOcjUIYGzojFW6bUUvLIyEdZarcbC0A2o17btWUaOhHcZOiZFoJhoLemyM5R07QW8OuuWXPxZIWXqXzDp7gLgk2RBCGB1Y2_8TpbwXnaCipf-DctIPnDQFZQfUzDGlGUa5m50v25CUyDV0uZW_Qpdr6JJQWUIvXa8fDBbtwT72_E65AO8OAJTl3TmYZTIOggHrSuBZ2uj-avATXqfCRg</recordid><startdate>201603</startdate><enddate>201603</enddate><creator>Yeh, Hung-Yueh</creator><creator>Serrano, Katherine Vargas</creator><creator>Acosta, Aimee Silvestry</creator><creator>Buhr, Richard J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>201603</creationdate><title>Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay</title><author>Yeh, Hung-Yueh ; Serrano, Katherine Vargas ; Acosta, Aimee Silvestry ; Buhr, Richard J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-98e3395cdab44868def035d7af9d15e1db1654a1cbe49f5a8f0b382debf2cd753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Automation</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriological Techniques - instrumentation</topic><topic>Bacteriological Techniques - methods</topic><topic>Base Sequence</topic><topic>chickens</topic><topic>Chickens - blood</topic><topic>Chickens - immunology</topic><topic>Flagellar proteins</topic><topic>FlgK</topic><topic>Immunoassay - methods</topic><topic>immunoassays</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella enterica - genetics</topic><topic>Salmonella enterica - immunology</topic><topic>Salmonella Infections, Animal - diagnosis</topic><topic>Salmonella Infections, Animal - immunology</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Simple Western</topic><topic>Zoonoses</topic><topic>Zoonoses - diagnosis</topic><topic>Zoonoses - immunology</topic><topic>Zoonoses - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeh, Hung-Yueh</creatorcontrib><creatorcontrib>Serrano, Katherine Vargas</creatorcontrib><creatorcontrib>Acosta, Aimee Silvestry</creatorcontrib><creatorcontrib>Buhr, Richard J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeh, Hung-Yueh</au><au>Serrano, Katherine Vargas</au><au>Acosta, Aimee Silvestry</au><au>Buhr, Richard J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2016-03</date><risdate>2016</risdate><volume>122</volume><spage>27</spage><epage>32</epage><pages>27-32</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called “Simple Western” was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera.
•Production of Salmonella flagellar protein FlgK•Immunization of chickens with the FlgK protein•Use of FlgK antibodies from chicken sera to develop automated capillary immunoassay</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26784737</pmid><doi>10.1016/j.mimet.2016.01.007</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2016-03, Vol.122, p.27-32 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2000319346 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals antibodies Antibodies, Bacterial - blood Automation Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - immunology Bacteriological Techniques - instrumentation Bacteriological Techniques - methods Base Sequence chickens Chickens - blood Chickens - immunology Flagellar proteins FlgK Immunoassay - methods immunoassays Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology Salmonella Salmonella enterica Salmonella enterica - genetics Salmonella enterica - immunology Salmonella Infections, Animal - diagnosis Salmonella Infections, Animal - immunology Salmonella Infections, Animal - microbiology Simple Western Zoonoses Zoonoses - diagnosis Zoonoses - immunology Zoonoses - microbiology |
| title | Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T21%3A10%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20of%20recombinant%20Salmonella%20flagellar%20protein,%20FlgK,%20and%20its%20uses%20in%20detection%20of%20anti-Salmonella%20antibodies%20in%20chickens%20by%20automated%20capillary%20immunoassay&rft.jtitle=Journal%20of%20microbiological%20methods&rft.au=Yeh,%20Hung-Yueh&rft.date=2016-03&rft.volume=122&rft.spage=27&rft.epage=32&rft.pages=27-32&rft.issn=0167-7012&rft.eissn=1872-8359&rft_id=info:doi/10.1016/j.mimet.2016.01.007&rft_dat=%3Cproquest_cross%3E1767069702%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1767069702&rft_id=info:pmid/26784737&rft_els_id=S0167701216300070&rfr_iscdi=true |