Effect of the expression of aquaporins 1 and 3 in mouse oocytes and compacted eight-cell embryos on the nucleation temperature for intracellular ice formation

The occurrence of intracellular ice formation (IIF) is the most important factor determining whether cells survive a cryopreservation procedure. What is not clear is the mechanism or route by which an external ice crystal can traverse the plasma membrane and cause the heterogeneous nucleation of the...

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Veröffentlicht in:	Reproduction (Cambridge, England) England), 2011-10, Vol.142 (4), p.505-515
Hauptverfasser:	Seki, Shinsuke, Edashige, Keisuke, Wada, Sakiko, Mazur, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aquaporin 1 Aquaporin 1 - metabolism Aquaporin 3 Aquaporin 3 - metabolism aquaporins Cell Membrane Cell Membrane - metabolism cryopreservation Cryoprotective Agents Cryoprotective Agents - pharmacology Crystallization cytology drug effects embryo (animal) Embryo, Mammalian Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Embryo, Mammalian - metabolism ethylene glycol Ethylene Glycol - pharmacology Female freezing Gene Expression Regulation, Developmental Gene Expression Regulation, Developmental - drug effects Hydrogen-Ion Concentration Ice ice nucleation metabolism Mice Mice, Inbred ICR Models, Animal Morula Morula - cytology Morula - drug effects Morula - metabolism oocytes Oocytes - cytology Oocytes - drug effects Oocytes - metabolism pharmacology plasma membrane RNA RNA, Double-Stranded RNA, Double-Stranded - pharmacology Temperature
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