Comparison of the in vitro toxicological activity of various particulate matter
Ultrafine particles (UFPs, < 2.5 µm) in air pollutants have been identified as a major cause of respiratory diseases, since they can affect the lung alveoli through the bronchus. In particular, if toxicants such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) are present in UFPs, they...
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| Veröffentlicht in: | Toxicology and industrial health 2018-02, Vol.34 (2), p.99-109 |
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| creator | Cho, Hyun-Ki Park, Chang-Gyun Shin, Han-Jae Park, Ki-Hong Lim, Heung-Bin |
| description | Ultrafine particles (UFPs, < 2.5 µm) in air pollutants have been identified as a major cause of respiratory diseases, since they can affect the lung alveoli through the bronchus. In particular, if toxicants such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) are present in UFPs, they can cause diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. This study compared in vitro toxicity of various particulate matter including UFPs from combustion particles of diesel (diesel exhaust particles (DEP)), rice straw (RS), pine stem (PS) and coal (CC), and road dust particles from tunnel (TD) and roadside (RD). UFPs from combustion particles and road dust were collected with a glass fiber filter using burning systems and a solid aerosol generator. Cell viability was determined by neutral red uptake assay using Chinese hamster ovary strain K1 cells. Redox cycling activity and intracellular reactive oxygen species were measured using 1,4-dithiothreitol (DTT) and 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, respectively. Our in vitro studies validated that combustion particles had high toxicological activity. PS demonstrated the highest activity in cytotoxicity but DEP had the highest activity in the DTT and DCF-DA assays. Overall, since the toxicological activity of particles generated by various means was different, risk assessment should be conducted through various toxicity evaluations rather than one toxicity evaluation. |
| doi_str_mv | 10.1177/0748233717749694 |
| format | Article |
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In particular, if toxicants such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) are present in UFPs, they can cause diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. This study compared in vitro toxicity of various particulate matter including UFPs from combustion particles of diesel (diesel exhaust particles (DEP)), rice straw (RS), pine stem (PS) and coal (CC), and road dust particles from tunnel (TD) and roadside (RD). UFPs from combustion particles and road dust were collected with a glass fiber filter using burning systems and a solid aerosol generator. Cell viability was determined by neutral red uptake assay using Chinese hamster ovary strain K1 cells. Redox cycling activity and intracellular reactive oxygen species were measured using 1,4-dithiothreitol (DTT) and 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, respectively. Our in vitro studies validated that combustion particles had high toxicological activity. PS demonstrated the highest activity in cytotoxicity but DEP had the highest activity in the DTT and DCF-DA assays. Overall, since the toxicological activity of particles generated by various means was different, risk assessment should be conducted through various toxicity evaluations rather than one toxicity evaluation.</description><identifier>ISSN: 0748-2337</identifier><identifier>EISSN: 1477-0393</identifier><identifier>DOI: 10.1177/0748233717749694</identifier><identifier>PMID: 29415641</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Air pollution ; Alveoli ; Assaying ; Asthma ; Bronchus ; Burning ; Chronic obstructive pulmonary disease ; Combustion ; Cytotoxicity ; Diesel ; Diesel engines ; Diesel fuels ; Dithiothreitol ; Dust ; Dust control ; Dust filters ; Glass fibers ; Heavy metals ; Lung cancer ; Lung diseases ; Obstructive lung disease ; Particulate matter ; Particulates ; Pollutants ; Polycyclic aromatic hydrocarbons ; Reactive oxygen species ; Redox properties ; Respiratory diseases ; Risk assessment ; Straw ; Toxicants ; Toxicity</subject><ispartof>Toxicology and industrial health, 2018-02, Vol.34 (2), p.99-109</ispartof><rights>The Author(s) 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-1d5858d8e9b2a0ea08473ab4d7b17d83e4373d795e47a6b27c5afef449e84ec33</citedby><cites>FETCH-LOGICAL-c365t-1d5858d8e9b2a0ea08473ab4d7b17d83e4373d795e47a6b27c5afef449e84ec33</cites><orcidid>0000-0002-4019-5370</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0748233717749694$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0748233717749694$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29415641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cho, Hyun-Ki</creatorcontrib><creatorcontrib>Park, Chang-Gyun</creatorcontrib><creatorcontrib>Shin, Han-Jae</creatorcontrib><creatorcontrib>Park, Ki-Hong</creatorcontrib><creatorcontrib>Lim, Heung-Bin</creatorcontrib><title>Comparison of the in vitro toxicological activity of various particulate matter</title><title>Toxicology and industrial health</title><addtitle>Toxicol Ind Health</addtitle><description>Ultrafine particles (UFPs, < 2.5 µm) in air pollutants have been identified as a major cause of respiratory diseases, since they can affect the lung alveoli through the bronchus. In particular, if toxicants such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) are present in UFPs, they can cause diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. This study compared in vitro toxicity of various particulate matter including UFPs from combustion particles of diesel (diesel exhaust particles (DEP)), rice straw (RS), pine stem (PS) and coal (CC), and road dust particles from tunnel (TD) and roadside (RD). UFPs from combustion particles and road dust were collected with a glass fiber filter using burning systems and a solid aerosol generator. Cell viability was determined by neutral red uptake assay using Chinese hamster ovary strain K1 cells. Redox cycling activity and intracellular reactive oxygen species were measured using 1,4-dithiothreitol (DTT) and 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, respectively. Our in vitro studies validated that combustion particles had high toxicological activity. PS demonstrated the highest activity in cytotoxicity but DEP had the highest activity in the DTT and DCF-DA assays. Overall, since the toxicological activity of particles generated by various means was different, risk assessment should be conducted through various toxicity evaluations rather than one toxicity evaluation.</description><subject>Air pollution</subject><subject>Alveoli</subject><subject>Assaying</subject><subject>Asthma</subject><subject>Bronchus</subject><subject>Burning</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Combustion</subject><subject>Cytotoxicity</subject><subject>Diesel</subject><subject>Diesel engines</subject><subject>Diesel fuels</subject><subject>Dithiothreitol</subject><subject>Dust</subject><subject>Dust control</subject><subject>Dust filters</subject><subject>Glass fibers</subject><subject>Heavy metals</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Obstructive lung disease</subject><subject>Particulate matter</subject><subject>Particulates</subject><subject>Pollutants</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Reactive oxygen species</subject><subject>Redox properties</subject><subject>Respiratory diseases</subject><subject>Risk assessment</subject><subject>Straw</subject><subject>Toxicants</subject><subject>Toxicity</subject><issn>0748-2337</issn><issn>1477-0393</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kM1LxDAQxYMouq7ePUnBi5dqvtokR1n8goW96Lmk6XSNtJs1SRf3vzdlVwXB0wwzv_dmeAhdEHxDiBC3WHBJGROp56pU_ABNCBcix0yxQzQZ1_m4P0GnIbxjjMuyoMfohCpOipKTCVrMXL_W3ga3ylybxTfI7Crb2OhdFt2nNa5zS2t0l2kTbZpvR2yTFG4IWVJGa4ZOR8h6HSP4M3TU6i7A-b5O0evD_cvsKZ8vHp9nd_PcsLKIOWkKWchGgqqpxqCx5ILpmjeiJqKRDDgTrBGqAC50WVNhCt1Cy7kCycEwNkXXO9-1dx8DhFj1NhjoOr2C9FlFlFKlZESShF79Qd_d4Ffpu4piTJnglI6GeEcZ70Lw0FZrb3vttxXB1Rh29TfsJLncGw91D82P4DvdBOQ7IOgl_F791_ALShqGjg</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Cho, Hyun-Ki</creator><creator>Park, Chang-Gyun</creator><creator>Shin, Han-Jae</creator><creator>Park, Ki-Hong</creator><creator>Lim, Heung-Bin</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T2</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4019-5370</orcidid></search><sort><creationdate>20180201</creationdate><title>Comparison of the in vitro toxicological activity of various particulate matter</title><author>Cho, Hyun-Ki ; Park, Chang-Gyun ; Shin, Han-Jae ; Park, Ki-Hong ; Lim, Heung-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-1d5858d8e9b2a0ea08473ab4d7b17d83e4373d795e47a6b27c5afef449e84ec33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Air pollution</topic><topic>Alveoli</topic><topic>Assaying</topic><topic>Asthma</topic><topic>Bronchus</topic><topic>Burning</topic><topic>Chronic obstructive pulmonary disease</topic><topic>Combustion</topic><topic>Cytotoxicity</topic><topic>Diesel</topic><topic>Diesel engines</topic><topic>Diesel fuels</topic><topic>Dithiothreitol</topic><topic>Dust</topic><topic>Dust control</topic><topic>Dust filters</topic><topic>Glass fibers</topic><topic>Heavy metals</topic><topic>Lung cancer</topic><topic>Lung diseases</topic><topic>Obstructive lung disease</topic><topic>Particulate matter</topic><topic>Particulates</topic><topic>Pollutants</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Reactive oxygen species</topic><topic>Redox properties</topic><topic>Respiratory diseases</topic><topic>Risk assessment</topic><topic>Straw</topic><topic>Toxicants</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cho, Hyun-Ki</creatorcontrib><creatorcontrib>Park, Chang-Gyun</creatorcontrib><creatorcontrib>Shin, Han-Jae</creatorcontrib><creatorcontrib>Park, Ki-Hong</creatorcontrib><creatorcontrib>Lim, Heung-Bin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology and industrial health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cho, Hyun-Ki</au><au>Park, Chang-Gyun</au><au>Shin, Han-Jae</au><au>Park, Ki-Hong</au><au>Lim, Heung-Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the in vitro toxicological activity of various particulate matter</atitle><jtitle>Toxicology and industrial health</jtitle><addtitle>Toxicol Ind Health</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>34</volume><issue>2</issue><spage>99</spage><epage>109</epage><pages>99-109</pages><issn>0748-2337</issn><eissn>1477-0393</eissn><abstract>Ultrafine particles (UFPs, < 2.5 µm) in air pollutants have been identified as a major cause of respiratory diseases, since they can affect the lung alveoli through the bronchus. In particular, if toxicants such as heavy metals and polycyclic aromatic hydrocarbons (PAHs) are present in UFPs, they can cause diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. This study compared in vitro toxicity of various particulate matter including UFPs from combustion particles of diesel (diesel exhaust particles (DEP)), rice straw (RS), pine stem (PS) and coal (CC), and road dust particles from tunnel (TD) and roadside (RD). UFPs from combustion particles and road dust were collected with a glass fiber filter using burning systems and a solid aerosol generator. Cell viability was determined by neutral red uptake assay using Chinese hamster ovary strain K1 cells. Redox cycling activity and intracellular reactive oxygen species were measured using 1,4-dithiothreitol (DTT) and 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, respectively. Our in vitro studies validated that combustion particles had high toxicological activity. PS demonstrated the highest activity in cytotoxicity but DEP had the highest activity in the DTT and DCF-DA assays. Overall, since the toxicological activity of particles generated by various means was different, risk assessment should be conducted through various toxicity evaluations rather than one toxicity evaluation.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>29415641</pmid><doi>10.1177/0748233717749694</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4019-5370</orcidid></addata></record> |
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| subjects | Air pollution Alveoli Assaying Asthma Bronchus Burning Chronic obstructive pulmonary disease Combustion Cytotoxicity Diesel Diesel engines Diesel fuels Dithiothreitol Dust Dust control Dust filters Glass fibers Heavy metals Lung cancer Lung diseases Obstructive lung disease Particulate matter Particulates Pollutants Polycyclic aromatic hydrocarbons Reactive oxygen species Redox properties Respiratory diseases Risk assessment Straw Toxicants Toxicity |
| title | Comparison of the in vitro toxicological activity of various particulate matter |
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