Expression of the large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase activase gene driven by rbcS promoter in Oryza sativa enhances the photosynthetic capacity
In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene p...
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| Veröffentlicht in: | Photosynthetica 2006-06, Vol.44 (2), p.208-214 |
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| creator | Wu, H. R. Ding, Z. S. Li, L. B. Jing, Y. X. Kuang, T. Y. |
| description | In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene promoter (rbcS) via Agrobacterium tumefaciens-mediated transformation. Transgenic rice plants were identified by polymerase chain reaction (PCR) and Southern and Western blot analyses. Net photosynthetic rate (PN) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth. |
| doi_str_mv | 10.1007/s11099-006-0008-7 |
| format | Article |
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R. ; Ding, Z. S. ; Li, L. B. ; Jing, Y. X. ; Kuang, T. Y.</creator><creatorcontrib>Wu, H. R. ; Ding, Z. S. ; Li, L. B. ; Jing, Y. X. ; Kuang, T. Y.</creatorcontrib><description>In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene promoter (rbcS) via Agrobacterium tumefaciens-mediated transformation. Transgenic rice plants were identified by polymerase chain reaction (PCR) and Southern and Western blot analyses. Net photosynthetic rate (PN) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth.</description><identifier>ISSN: 0300-3604</identifier><identifier>EISSN: 1573-9058</identifier><identifier>DOI: 10.1007/s11099-006-0008-7</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Agrobacterium ; Carboxylation ; Cultivars ; Electron transport ; Genetic transformation ; Oryza sativa ; Oxygenase ; Photochemicals ; Photosynthesis ; Photosystem ; Plant growth ; Polymerase chain reaction ; Ribulose-1,5-bisphosphate ; Rice ; Transgenic plants</subject><ispartof>Photosynthetica, 2006-06, Vol.44 (2), p.208-214</ispartof><rights>Institute of Experimental Botany, ASCR 2006.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-e34cdc28ec766e46f032ae5de67c66d8783d58eae4d844757e54c5a91b9bfaa03</citedby><cites>FETCH-LOGICAL-c347t-e34cdc28ec766e46f032ae5de67c66d8783d58eae4d844757e54c5a91b9bfaa03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Wu, H. 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Net photosynthetic rate (PN) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth.</description><subject>Agrobacterium</subject><subject>Carboxylation</subject><subject>Cultivars</subject><subject>Electron transport</subject><subject>Genetic transformation</subject><subject>Oryza sativa</subject><subject>Oxygenase</subject><subject>Photochemicals</subject><subject>Photosynthesis</subject><subject>Photosystem</subject><subject>Plant growth</subject><subject>Polymerase chain reaction</subject><subject>Ribulose-1,5-bisphosphate</subject><subject>Rice</subject><subject>Transgenic plants</subject><issn>0300-3604</issn><issn>1573-9058</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpdkcGOFCEQhonRxHH1AbyRmHgSF4YGmqPZ7KrJJntQz4Smq3fY9EBLMZtt38l3lHY8eajUX-Sj6k9-Qt4K_lFwbi5RCG4t41y34j0zz8hOKCOZ5ap_TnZccs6k5t1L8grxgfP2S8od-X39tBRAjDnRPNF6ADr7cg80Yp5yOW6PJQ6nOSMw8UGxIeJyyK18BRp8GfLTOnuEy9bvITVFfajxcRNtBjqW-AiJDistQ_hGl5KPuUKhMdG7sv7yFP2GU0gHnwLgXw_tRM24pqZrDO3O4kOs62vyYvIzwpt__YL8uLn-fvWF3d59_nr16ZYF2ZnKQHZhDPsegtEaOj1xufegRtAmaD32ppej6sFDN_ZdZ5QB1QXlrRjsMHnP5QV5f97b3P48AVZ3jBhgnn2CfEInrLWqt7aB7_4DH_KppObN7bVuu7nWslHiTIWSEQtMbinx6MvqBHdbfO4cn2vxuS0-Z-Qf_46SRA</recordid><startdate>200606</startdate><enddate>200606</enddate><creator>Wu, H. 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Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase activase gene driven by rbcS promoter in Oryza sativa enhances the photosynthetic capacity</atitle><jtitle>Photosynthetica</jtitle><date>2006-06</date><risdate>2006</risdate><volume>44</volume><issue>2</issue><spage>208</spage><epage>214</epage><pages>208-214</pages><issn>0300-3604</issn><eissn>1573-9058</eissn><abstract>In order to investigate the effect of large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activase (RuBPCO-A) on photosynthesis, cDNA of the enzyme (rca) was transferred to rice cultivars (Oryza sativa f. japonica cv. Nipponbare) under the control of RuBPCO small subunit gene promoter (rbcS) via Agrobacterium tumefaciens-mediated transformation. Transgenic rice plants were identified by polymerase chain reaction (PCR) and Southern and Western blot analyses. Net photosynthetic rate (PN) values of the T1 transgenic lines 34 (T34) and 40 (T40) were 45.26 and 46.32 % higher than that of the control plants, respectively. At the same time, their carboxylation efficiency and RuBPCO initial activity, quantum yield of electron transport in photosystem 2 (ΦPS2), and steady state photochemical fluorescence quenching (qP) increased. In addition, heading time of the transgenic rice was advanced. Thus increasing the amount of large isoform of RuBPCO-A in the transgenic rice might have a stimulatory effect on both photosynthesis and plant growth.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1007/s11099-006-0008-7</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agrobacterium Carboxylation Cultivars Electron transport Genetic transformation Oryza sativa Oxygenase Photochemicals Photosynthesis Photosystem Plant growth Polymerase chain reaction Ribulose-1,5-bisphosphate Rice Transgenic plants |
| title | Expression of the large isoform of ribulose-1,5-bisphosphate carboxylase/oxygenase activase gene driven by rbcS promoter in Oryza sativa enhances the photosynthetic capacity |
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