Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain
To survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. A survey of 50 different samples of fresh marine fish (conger, swordfish, sole, grouper and whiting) was conducted over a period of 5 months. Trays of fillets an...
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| Veröffentlicht in: | Journal of applied microbiology 2006-03, Vol.100 (3), p.527-536 |
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| creator | Herrera, F.C Santos, J.A Otero, A García-López, M.-L |
| description | To survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. A survey of 50 different samples of fresh marine fish (conger, swordfish, sole, grouper and whiting) was conducted over a period of 5 months. Trays of fillets and steaks were obtained at retail level and tested for foodborne bacterial pathogens. Vibrio cholerae and Salmonella were not detected. Two samples (4%) yielded Vibrio strains carrying a DNA fragment specific for Vibrio parahaemolyticus, but resulted negative to PCR amplification of the virulence-related tdh gene. Levels of motile Aeromonas ranging from 2·29 to 7·20 log CFU g[superscript [-]1] were found in 31 (62%) samples. All fish portions were positive for the Aeromonas hlyA gene and 38 for both aerA and hlyA genes, which may contribute to diarrhoea-related virulence. The incidence of Listeria monocytogenes was 10%. Levels of Staphylococcus aureus lower than 2 log CFU g[superscript [-]1] were found in 15 (30%) samples. Numbers of presumptive Clostridium perfringens ranging from 1·82 ± 0·22 to 4·26 ± 1·25 log CFU g[superscript [-]1] were detected in 42 (84%) samples. Edwardsiella tarda was detected in two samples of grouper fillets. Displayed portions of raw fish carried bacteria that can cause foodborne disease. The risk posed by fresh fish when properly cooked is low, but high when destined to be consumed raw, undercooked or very lightly processed. This study revealed that raw fish sold in Spain could be a source of foodborne bacterial pathogens. Improvements in handling and processing are needed to minimize the prevalence of pathogenic bacteria. |
| doi_str_mv | 10.1111/j.1365-2672.2005.02848.x |
| format | Article |
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A survey of 50 different samples of fresh marine fish (conger, swordfish, sole, grouper and whiting) was conducted over a period of 5 months. Trays of fillets and steaks were obtained at retail level and tested for foodborne bacterial pathogens. Vibrio cholerae and Salmonella were not detected. Two samples (4%) yielded Vibrio strains carrying a DNA fragment specific for Vibrio parahaemolyticus, but resulted negative to PCR amplification of the virulence-related tdh gene. Levels of motile Aeromonas ranging from 2·29 to 7·20 log CFU g[superscript [-]1] were found in 31 (62%) samples. All fish portions were positive for the Aeromonas hlyA gene and 38 for both aerA and hlyA genes, which may contribute to diarrhoea-related virulence. The incidence of Listeria monocytogenes was 10%. Levels of Staphylococcus aureus lower than 2 log CFU g[superscript [-]1] were found in 15 (30%) samples. Numbers of presumptive Clostridium perfringens ranging from 1·82 ± 0·22 to 4·26 ± 1·25 log CFU g[superscript [-]1] were detected in 42 (84%) samples. Edwardsiella tarda was detected in two samples of grouper fillets. Displayed portions of raw fish carried bacteria that can cause foodborne disease. The risk posed by fresh fish when properly cooked is low, but high when destined to be consumed raw, undercooked or very lightly processed. This study revealed that raw fish sold in Spain could be a source of foodborne bacterial pathogens. Improvements in handling and processing are needed to minimize the prevalence of pathogenic bacteria.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2005.02848.x</identifier><identifier>PMID: 16478492</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject><![CDATA[Aeromonas ; Aeromonas - genetics ; Aeromonas - isolation & purification ; Animals ; Bacteria ; Biological and medical sciences ; Clostridium perfringens ; Clostridium perfringens - isolation & purification ; DNA ; Edwardsiella ; Edwardsiella tarda ; fillets ; Fishes - microbiology ; Food Handling - methods ; Food Microbiology ; foodborne bacteria ; foodborne illness ; Fundamental and applied biological sciences. Psychology ; genes ; Genes, Bacterial - genetics ; Genotype ; grouper ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - isolation & purification ; marine fish ; Microbiology ; pathogens ; Pisces ; polymerase chain reaction ; raw fish ; risk ; Salmonella ; Salmonella - isolation & purification ; Spain ; Staphylococcus aureus ; Staphylococcus aureus - isolation & purification ; steaks ; surveys ; trays ; Vibrio - isolation & purification ; Vibrio cholerae ; Vibrio parahaemolyticus ; virulence ; Virulence - genetics ; whiting ; Xiphias gladius]]></subject><ispartof>Journal of applied microbiology, 2006-03, Vol.100 (3), p.527-536</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5298-eb27e48d0dfc6e40a24dc89a2b8feb4db12f2ea9bbaca87ce72b7ceec6ef8f123</citedby><cites>FETCH-LOGICAL-c5298-eb27e48d0dfc6e40a24dc89a2b8feb4db12f2ea9bbaca87ce72b7ceec6ef8f123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2005.02848.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2005.02848.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17503548$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16478492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herrera, F.C</creatorcontrib><creatorcontrib>Santos, J.A</creatorcontrib><creatorcontrib>Otero, A</creatorcontrib><creatorcontrib>García-López, M.-L</creatorcontrib><title>Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. A survey of 50 different samples of fresh marine fish (conger, swordfish, sole, grouper and whiting) was conducted over a period of 5 months. Trays of fillets and steaks were obtained at retail level and tested for foodborne bacterial pathogens. Vibrio cholerae and Salmonella were not detected. Two samples (4%) yielded Vibrio strains carrying a DNA fragment specific for Vibrio parahaemolyticus, but resulted negative to PCR amplification of the virulence-related tdh gene. Levels of motile Aeromonas ranging from 2·29 to 7·20 log CFU g[superscript [-]1] were found in 31 (62%) samples. All fish portions were positive for the Aeromonas hlyA gene and 38 for both aerA and hlyA genes, which may contribute to diarrhoea-related virulence. The incidence of Listeria monocytogenes was 10%. Levels of Staphylococcus aureus lower than 2 log CFU g[superscript [-]1] were found in 15 (30%) samples. Numbers of presumptive Clostridium perfringens ranging from 1·82 ± 0·22 to 4·26 ± 1·25 log CFU g[superscript [-]1] were detected in 42 (84%) samples. Edwardsiella tarda was detected in two samples of grouper fillets. Displayed portions of raw fish carried bacteria that can cause foodborne disease. The risk posed by fresh fish when properly cooked is low, but high when destined to be consumed raw, undercooked or very lightly processed. This study revealed that raw fish sold in Spain could be a source of foodborne bacterial pathogens. Improvements in handling and processing are needed to minimize the prevalence of pathogenic bacteria.</description><subject>Aeromonas</subject><subject>Aeromonas - genetics</subject><subject>Aeromonas - isolation & purification</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - isolation & purification</subject><subject>DNA</subject><subject>Edwardsiella</subject><subject>Edwardsiella tarda</subject><subject>fillets</subject><subject>Fishes - microbiology</subject><subject>Food Handling - methods</subject><subject>Food Microbiology</subject><subject>foodborne bacteria</subject><subject>foodborne illness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genotype</subject><subject>grouper</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>marine fish</subject><subject>Microbiology</subject><subject>pathogens</subject><subject>Pisces</subject><subject>polymerase chain reaction</subject><subject>raw fish</subject><subject>risk</subject><subject>Salmonella</subject><subject>Salmonella - isolation & purification</subject><subject>Spain</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>steaks</subject><subject>surveys</subject><subject>trays</subject><subject>Vibrio - isolation & purification</subject><subject>Vibrio cholerae</subject><subject>Vibrio parahaemolyticus</subject><subject>virulence</subject><subject>Virulence - genetics</subject><subject>whiting</subject><subject>Xiphias gladius</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhiMEoqXwChAhlVuC7diJc-BQVUCLinooPVtjZ7z1krWDnYj27XG6Kypxwgd7JH__zPgfF0VJSU3z-ritadOKirUdqxkhoiZMclnfPyuO_148f4x5JUjHjopXKW0JoQ0R7cviiLa8k7xnx4W-NmaJEb3BMtjShjDoED2WE8x3YYPemVKDmTE6KJ0vI87gxnKKOIH5CRscyinE2QWfVv0Oosti69LdSt9M4Pzr4oWFMeGbw3lS3H75_OP8orq6_np5fnZVGcF6WaFmHXI5kMGaFjkBxgcje2BaWtR80JRZhtDr3A7IzmDHdN4xw1ZaypqT4sM-7xTDrwXTrHYuGRxH8BiWpGjf91y2TQbf_wNuwxJ97k2xhmWGC5ohuYdMDClFtGqKLj_vQVGi1iGorVq9VqvXah2CehyCus_St4f8i97h8CQ8uJ6B0wMAycBoI3jj0hPXCdIILjP3ac_9diM-_HcD6tvZ9zXK-nd7vYWgYBNzjdsbRqjIP4GIvumbPzjKraQ</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Herrera, F.C</creator><creator>Santos, J.A</creator><creator>Otero, A</creator><creator>García-López, M.-L</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T2</scope><scope>7U2</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope></search><sort><creationdate>200603</creationdate><title>Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain</title><author>Herrera, F.C ; Santos, J.A ; Otero, A ; García-López, M.-L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5298-eb27e48d0dfc6e40a24dc89a2b8feb4db12f2ea9bbaca87ce72b7ceec6ef8f123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aeromonas</topic><topic>Aeromonas - genetics</topic><topic>Aeromonas - isolation & purification</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - isolation & purification</topic><topic>DNA</topic><topic>Edwardsiella</topic><topic>Edwardsiella tarda</topic><topic>fillets</topic><topic>Fishes - microbiology</topic><topic>Food Handling - methods</topic><topic>Food Microbiology</topic><topic>foodborne bacteria</topic><topic>foodborne illness</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genotype</topic><topic>grouper</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>marine fish</topic><topic>Microbiology</topic><topic>pathogens</topic><topic>Pisces</topic><topic>polymerase chain reaction</topic><topic>raw fish</topic><topic>risk</topic><topic>Salmonella</topic><topic>Salmonella - isolation & purification</topic><topic>Spain</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>steaks</topic><topic>surveys</topic><topic>trays</topic><topic>Vibrio - isolation & purification</topic><topic>Vibrio cholerae</topic><topic>Vibrio parahaemolyticus</topic><topic>virulence</topic><topic>Virulence - genetics</topic><topic>whiting</topic><topic>Xiphias gladius</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herrera, F.C</creatorcontrib><creatorcontrib>Santos, J.A</creatorcontrib><creatorcontrib>Otero, A</creatorcontrib><creatorcontrib>García-López, M.-L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herrera, F.C</au><au>Santos, J.A</au><au>Otero, A</au><au>García-López, M.-L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2006-03</date><risdate>2006</risdate><volume>100</volume><issue>3</issue><spage>527</spage><epage>536</epage><pages>527-536</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>To survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish. A survey of 50 different samples of fresh marine fish (conger, swordfish, sole, grouper and whiting) was conducted over a period of 5 months. Trays of fillets and steaks were obtained at retail level and tested for foodborne bacterial pathogens. Vibrio cholerae and Salmonella were not detected. Two samples (4%) yielded Vibrio strains carrying a DNA fragment specific for Vibrio parahaemolyticus, but resulted negative to PCR amplification of the virulence-related tdh gene. Levels of motile Aeromonas ranging from 2·29 to 7·20 log CFU g[superscript [-]1] were found in 31 (62%) samples. All fish portions were positive for the Aeromonas hlyA gene and 38 for both aerA and hlyA genes, which may contribute to diarrhoea-related virulence. The incidence of Listeria monocytogenes was 10%. Levels of Staphylococcus aureus lower than 2 log CFU g[superscript [-]1] were found in 15 (30%) samples. Numbers of presumptive Clostridium perfringens ranging from 1·82 ± 0·22 to 4·26 ± 1·25 log CFU g[superscript [-]1] were detected in 42 (84%) samples. Edwardsiella tarda was detected in two samples of grouper fillets. Displayed portions of raw fish carried bacteria that can cause foodborne disease. The risk posed by fresh fish when properly cooked is low, but high when destined to be consumed raw, undercooked or very lightly processed. This study revealed that raw fish sold in Spain could be a source of foodborne bacterial pathogens. Improvements in handling and processing are needed to minimize the prevalence of pathogenic bacteria.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16478492</pmid><doi>10.1111/j.1365-2672.2005.02848.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2006-03, Vol.100 (3), p.527-536 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aeromonas Aeromonas - genetics Aeromonas - isolation & purification Animals Bacteria Biological and medical sciences Clostridium perfringens Clostridium perfringens - isolation & purification DNA Edwardsiella Edwardsiella tarda fillets Fishes - microbiology Food Handling - methods Food Microbiology foodborne bacteria foodborne illness Fundamental and applied biological sciences. Psychology genes Genes, Bacterial - genetics Genotype grouper Listeria Listeria monocytogenes Listeria monocytogenes - isolation & purification marine fish Microbiology pathogens Pisces polymerase chain reaction raw fish risk Salmonella Salmonella - isolation & purification Spain Staphylococcus aureus Staphylococcus aureus - isolation & purification steaks surveys trays Vibrio - isolation & purification Vibrio cholerae Vibrio parahaemolyticus virulence Virulence - genetics whiting Xiphias gladius |
| title | Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain |
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