Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains
Bones are often recovered in forensic investigations, including missing persons and mass disasters. While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TBone Ex buffer (DNA Chip Research Inc.) digests bone chips without powdering. In this study,...
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| Veröffentlicht in: | Journal of forensic sciences 2018-11, Vol.63 (6), p.1819-1823 |
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| container_issue | 6 |
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| container_title | Journal of forensic sciences |
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| creator | Harrel, Michelle Mayes, Carrie Gangitano, David Hughes‐Stamm, Sheree |
| description | Bones are often recovered in forensic investigations, including missing persons and mass disasters. While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TBone Ex buffer (DNA Chip Research Inc.) digests bone chips without powdering. In this study, six bones were extracted using the TBone Ex kit in conjunction with the PrepFiler® BTA™ DNA extraction kit (Thermo Fisher Scientific) both manually and via an automated platform. Comparable amounts of DNA were recovered from a 50 mg bone chip using the TBone Ex kit and 50 mg of powdered bone with the PrepFiler® BTA™ kit. However, automated DNA purification decreased DNA yield (p < 0.05). Nevertheless, short tandem repeat (STR) success was comparable across all methods tested. This study demonstrates that digestion of whole bone fragments is an efficient alternative to powdering bones for DNA extraction without compromising downstream STR profile quality. |
| doi_str_mv | 10.1111/1556-4029.13749 |
| format | Article |
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While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TBone Ex buffer (DNA Chip Research Inc.) digests bone chips without powdering. In this study, six bones were extracted using the TBone Ex kit in conjunction with the PrepFiler® BTA™ DNA extraction kit (Thermo Fisher Scientific) both manually and via an automated platform. Comparable amounts of DNA were recovered from a 50 mg bone chip using the TBone Ex kit and 50 mg of powdered bone with the PrepFiler® BTA™ kit. However, automated DNA purification decreased DNA yield (p < 0.05). Nevertheless, short tandem repeat (STR) success was comparable across all methods tested. This study demonstrates that digestion of whole bone fragments is an efficient alternative to powdering bones for DNA extraction without compromising downstream STR profile quality.</description><identifier>ISSN: 0022-1198</identifier><identifier>EISSN: 1556-4029</identifier><identifier>DOI: 10.1111/1556-4029.13749</identifier><identifier>PMID: 29411392</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Automation ; Body Remains ; bone ; Bone and Bones - chemistry ; Bone Demineralization Technique - methods ; Bones ; Deoxyribonucleic acid ; Disasters ; DNA ; DNA - isolation & purification ; DNA extraction ; DNA Fingerprinting - methods ; forensic biology ; forensic science ; Human remains ; Humans ; Microsatellite Repeats ; Powdering ; Purification ; Real-Time Polymerase Chain Reaction ; short tandem repeats</subject><ispartof>Journal of forensic sciences, 2018-11, Vol.63 (6), p.1819-1823</ispartof><rights>2018 American Academy of Forensic Sciences</rights><rights>2018 American Academy of Forensic Sciences.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3719-640c2864ad925a852c2be208b08a810a6016e113338e140935076530d5e891a53</citedby><cites>FETCH-LOGICAL-c3719-640c2864ad925a852c2be208b08a810a6016e113338e140935076530d5e891a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1556-4029.13749$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1556-4029.13749$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29411392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harrel, Michelle</creatorcontrib><creatorcontrib>Mayes, Carrie</creatorcontrib><creatorcontrib>Gangitano, David</creatorcontrib><creatorcontrib>Hughes‐Stamm, Sheree</creatorcontrib><title>Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>Bones are often recovered in forensic investigations, including missing persons and mass disasters. While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TBone Ex buffer (DNA Chip Research Inc.) digests bone chips without powdering. In this study, six bones were extracted using the TBone Ex kit in conjunction with the PrepFiler® BTA™ DNA extraction kit (Thermo Fisher Scientific) both manually and via an automated platform. Comparable amounts of DNA were recovered from a 50 mg bone chip using the TBone Ex kit and 50 mg of powdered bone with the PrepFiler® BTA™ kit. However, automated DNA purification decreased DNA yield (p < 0.05). Nevertheless, short tandem repeat (STR) success was comparable across all methods tested. This study demonstrates that digestion of whole bone fragments is an efficient alternative to powdering bones for DNA extraction without compromising downstream STR profile quality.</description><subject>Automation</subject><subject>Body Remains</subject><subject>bone</subject><subject>Bone and Bones - chemistry</subject><subject>Bone Demineralization Technique - methods</subject><subject>Bones</subject><subject>Deoxyribonucleic acid</subject><subject>Disasters</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA extraction</subject><subject>DNA Fingerprinting - methods</subject><subject>forensic biology</subject><subject>forensic science</subject><subject>Human remains</subject><subject>Humans</subject><subject>Microsatellite Repeats</subject><subject>Powdering</subject><subject>Purification</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>short tandem repeats</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EglKY2VAkFpaUOzt24rEqLQUViviYLTe5ikDaFDvhY-Mn8Bv5JaQUGFi45aTTc69ePYztIXSwmSOUUoURcN1BEUd6jbV-L-usBcB5iKiTLbbt_T0AKFS4yba4jhCF5i027D_ZorZVXs6D8TToBpflc0bu4-194IiC44tu0H-pnE2_iHOq7sosGJQuuH6ggipbBFc0s_nc77CNqS087X7vNrsd9G96w3A0PjntdUdhKmLUoYog5YmKbKa5tInkKZ8Qh2QCiU0QrAJU1HQTIiGMQAsJsZICMkmJRitFmx2ucheufKzJV2aW-5SKws6prL1BrTUHiARv0IM_6H1Zu3nTznDkcawQImyooxWVutJ7R1OzcPnMuleDYJaSzVKpWSo1X5Kbj_3v3Hoyo-yX_7HaAHIFPOcFvf6XZ84G41XwJ_ligm4</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Harrel, Michelle</creator><creator>Mayes, Carrie</creator><creator>Gangitano, David</creator><creator>Hughes‐Stamm, Sheree</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope></search><sort><creationdate>201811</creationdate><title>Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains</title><author>Harrel, Michelle ; Mayes, Carrie ; Gangitano, David ; Hughes‐Stamm, Sheree</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3719-640c2864ad925a852c2be208b08a810a6016e113338e140935076530d5e891a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Automation</topic><topic>Body Remains</topic><topic>bone</topic><topic>Bone and Bones - chemistry</topic><topic>Bone Demineralization Technique - methods</topic><topic>Bones</topic><topic>Deoxyribonucleic acid</topic><topic>Disasters</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA extraction</topic><topic>DNA Fingerprinting - methods</topic><topic>forensic biology</topic><topic>forensic science</topic><topic>Human remains</topic><topic>Humans</topic><topic>Microsatellite Repeats</topic><topic>Powdering</topic><topic>Purification</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>short tandem repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harrel, Michelle</creatorcontrib><creatorcontrib>Mayes, Carrie</creatorcontrib><creatorcontrib>Gangitano, David</creatorcontrib><creatorcontrib>Hughes‐Stamm, Sheree</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harrel, Michelle</au><au>Mayes, Carrie</au><au>Gangitano, David</au><au>Hughes‐Stamm, Sheree</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2018-11</date><risdate>2018</risdate><volume>63</volume><issue>6</issue><spage>1819</spage><epage>1823</epage><pages>1819-1823</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><abstract>Bones are often recovered in forensic investigations, including missing persons and mass disasters. While traditional DNA extraction methods rely on grinding bone into powder prior to DNA purification, the TBone Ex buffer (DNA Chip Research Inc.) digests bone chips without powdering. In this study, six bones were extracted using the TBone Ex kit in conjunction with the PrepFiler® BTA™ DNA extraction kit (Thermo Fisher Scientific) both manually and via an automated platform. Comparable amounts of DNA were recovered from a 50 mg bone chip using the TBone Ex kit and 50 mg of powdered bone with the PrepFiler® BTA™ kit. However, automated DNA purification decreased DNA yield (p < 0.05). Nevertheless, short tandem repeat (STR) success was comparable across all methods tested. This study demonstrates that digestion of whole bone fragments is an efficient alternative to powdering bones for DNA extraction without compromising downstream STR profile quality.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29411392</pmid><doi>10.1111/1556-4029.13749</doi><tpages>5</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Automation Body Remains bone Bone and Bones - chemistry Bone Demineralization Technique - methods Bones Deoxyribonucleic acid Disasters DNA DNA - isolation & purification DNA extraction DNA Fingerprinting - methods forensic biology forensic science Human remains Humans Microsatellite Repeats Powdering Purification Real-Time Polymerase Chain Reaction short tandem repeats |
| title | Evaluation Of A Powder‐Free DNA Extraction Method For Skeletal Remains |
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