Analysis of the Spanish CCR5-∆32 inventory of cord blood units: lower cell counts in homozygous donors
The possibility to use CCR5 -∆32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the CCR5 -∆32 mutation. To achiev...
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| Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2018-06, Vol.53 (6), p.741-748 |
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| creator | Enrich, Emma Vidal, Francisco Sánchez-Gordo, Francisco Gómez-Zumaquero, Juan M. Balas, Antonio Rudilla, Francesc Barea, Luisa Castro, Ana Larrea, Luis Perez-Vaquero, Miguel A. Prat, Isidro Querol, Sergio Garrido, Gregorio Matesanz, Rafael Carreras, Enric Duarte, Rafael F. |
| description | The possibility to use
CCR5
-∆32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the
CCR5
-∆32 mutation. To achieve an inventory of
CCR5
-∆32 cord blood units, the 20,236 best cell quality units of the Spanish Registry were genotyped. Furthermore, their CD34
+
and total nucleated cells counts, blood type, gender, HLA and donor’s geographical and ancestral origin were analyzed. The results showed 130 (0.64%) units homozygous for the deletion, 2,646 (13.08%) heterozygous and 17,460 (86.28%) did not present the mutation. Interestingly, a significant lower amount of CD34
+
cells was found in the
CCR5
-∆32 homozygous units. In addition, a significant association was found among donor’s ancestral origin and the mutation, with a higher percentage of CCR5-∆32 units with a European ancestry. In summary, identification of a relatively high number of
CCR5
-∆32 units is feasible and will facilitate the development of clinical trials for HIV cure in patients requiring hematopoietic transplantation. Further studies are required to understand the significance of lower cell counts within the
CCR5
-∆32 homozygous group and its clinical impact. |
| doi_str_mv | 10.1038/s41409-018-0114-8 |
| format | Article |
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CCR5
-∆32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the
CCR5
-∆32 mutation. To achieve an inventory of
CCR5
-∆32 cord blood units, the 20,236 best cell quality units of the Spanish Registry were genotyped. Furthermore, their CD34
+
and total nucleated cells counts, blood type, gender, HLA and donor’s geographical and ancestral origin were analyzed. The results showed 130 (0.64%) units homozygous for the deletion, 2,646 (13.08%) heterozygous and 17,460 (86.28%) did not present the mutation. Interestingly, a significant lower amount of CD34
+
cells was found in the
CCR5
-∆32 homozygous units. In addition, a significant association was found among donor’s ancestral origin and the mutation, with a higher percentage of CCR5-∆32 units with a European ancestry. In summary, identification of a relatively high number of
CCR5
-∆32 units is feasible and will facilitate the development of clinical trials for HIV cure in patients requiring hematopoietic transplantation. Further studies are required to understand the significance of lower cell counts within the
CCR5
-∆32 homozygous group and its clinical impact.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/s41409-018-0114-8</identifier><identifier>PMID: 29410548</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45 ; 45/77 ; 631/250/1904 ; 692/308/2171 ; Analysis ; Blood groups ; Care and treatment ; CCR5 protein ; CD34 antigen ; Cell Biology ; Chemokine receptors ; Clinical trials ; Clonal deletion ; Cord blood ; Cord Blood Stem Cell Transplantation - methods ; Female ; Fetal blood ; Gene deletion ; Genotype ; Hematology ; Histocompatibility antigen HLA ; HIV ; HIV infections ; HIV patients ; Homozygote ; Human immunodeficiency virus ; Humans ; Infection ; Internal Medicine ; Male ; Medical research ; Medicine ; Medicine & Public Health ; Mutation ; Patients ; Public Health ; Receptors, CCR5 - immunology ; Stem cell transplantation ; Stem Cells ; Surgery ; Tissue Donors ; Transplantation ; Transplants & implants ; Umbilical cord</subject><ispartof>Bone marrow transplantation (Basingstoke), 2018-06, Vol.53 (6), p.741-748</ispartof><rights>Macmillan Publishers Limited, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3198-466d0cb5356ed782007c77b11d5e2424be34bee6697c861197e625019229bf113</citedby><cites>FETCH-LOGICAL-c3198-466d0cb5356ed782007c77b11d5e2424be34bee6697c861197e625019229bf113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41409-018-0114-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41409-018-0114-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29410548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Enrich, Emma</creatorcontrib><creatorcontrib>Vidal, Francisco</creatorcontrib><creatorcontrib>Sánchez-Gordo, Francisco</creatorcontrib><creatorcontrib>Gómez-Zumaquero, Juan M.</creatorcontrib><creatorcontrib>Balas, Antonio</creatorcontrib><creatorcontrib>Rudilla, Francesc</creatorcontrib><creatorcontrib>Barea, Luisa</creatorcontrib><creatorcontrib>Castro, Ana</creatorcontrib><creatorcontrib>Larrea, Luis</creatorcontrib><creatorcontrib>Perez-Vaquero, Miguel A.</creatorcontrib><creatorcontrib>Prat, Isidro</creatorcontrib><creatorcontrib>Querol, Sergio</creatorcontrib><creatorcontrib>Garrido, Gregorio</creatorcontrib><creatorcontrib>Matesanz, Rafael</creatorcontrib><creatorcontrib>Carreras, Enric</creatorcontrib><creatorcontrib>Duarte, Rafael F.</creatorcontrib><title>Analysis of the Spanish CCR5-∆32 inventory of cord blood units: lower cell counts in homozygous donors</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><addtitle>Bone Marrow Transplant</addtitle><description>The possibility to use
CCR5
-∆32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the
CCR5
-∆32 mutation. To achieve an inventory of
CCR5
-∆32 cord blood units, the 20,236 best cell quality units of the Spanish Registry were genotyped. Furthermore, their CD34
+
and total nucleated cells counts, blood type, gender, HLA and donor’s geographical and ancestral origin were analyzed. The results showed 130 (0.64%) units homozygous for the deletion, 2,646 (13.08%) heterozygous and 17,460 (86.28%) did not present the mutation. Interestingly, a significant lower amount of CD34
+
cells was found in the
CCR5
-∆32 homozygous units. In addition, a significant association was found among donor’s ancestral origin and the mutation, with a higher percentage of CCR5-∆32 units with a European ancestry. In summary, identification of a relatively high number of
CCR5
-∆32 units is feasible and will facilitate the development of clinical trials for HIV cure in patients requiring hematopoietic transplantation. Further studies are required to understand the significance of lower cell counts within the
CCR5
-∆32 homozygous group and its clinical impact.</description><subject>45</subject><subject>45/77</subject><subject>631/250/1904</subject><subject>692/308/2171</subject><subject>Analysis</subject><subject>Blood groups</subject><subject>Care and treatment</subject><subject>CCR5 protein</subject><subject>CD34 antigen</subject><subject>Cell Biology</subject><subject>Chemokine receptors</subject><subject>Clinical trials</subject><subject>Clonal deletion</subject><subject>Cord blood</subject><subject>Cord Blood Stem Cell Transplantation - methods</subject><subject>Female</subject><subject>Fetal blood</subject><subject>Gene deletion</subject><subject>Genotype</subject><subject>Hematology</subject><subject>Histocompatibility antigen HLA</subject><subject>HIV</subject><subject>HIV infections</subject><subject>HIV patients</subject><subject>Homozygote</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Infection</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mutation</subject><subject>Patients</subject><subject>Public Health</subject><subject>Receptors, CCR5 - immunology</subject><subject>Stem cell transplantation</subject><subject>Stem Cells</subject><subject>Surgery</subject><subject>Tissue Donors</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><subject>Umbilical cord</subject><issn>0268-3369</issn><issn>1476-5365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kcmKFEEQhhNRnHb0AbxIgiBeaszItdJb04wLDAgu56SWrK4aqjPbjKqRngcQn9MnMYueGRfwEMQhvvj5I35CngI7AybKVyhBMlswKHOBLMp7ZAXS6EIJre6TFeO6LITQ9oQ8QrxkDKRk6iE54VYCU7JckX4dqvGAA9LY0an39NO-CgP2dLP5qIqfP74LTodw5cMU02FhmphaWo8xtnQOw4Sv6Ri_-UQbP455OIcJ8wLt4y5eH7ZxRtrGEBM-Jg-6akT_5Kafki9vzj9v3hUXH96-36wvikaALQupdcuaWgmlfWtKzphpjKkBWuW55LL2IpfX2pqm1ADWeM0VA8u5rTsAcUpeHnX3KX6dPU5uN-Birgo-u3Fgrc20UGVGn_-DXsY55X-g40xpo7jNb76jttXo3RC6OKWqWUTdWhlupDVGZ-rFH1Tvq3HqMY7zNMSAf4NwBJsUEZPv3D4NuyodHDC3pOqOqbqcqltSdYvRZzdG53rn27uN2xgzwI8A5lHY-vT7kv-r_gIAKal8</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Enrich, Emma</creator><creator>Vidal, Francisco</creator><creator>Sánchez-Gordo, Francisco</creator><creator>Gómez-Zumaquero, Juan M.</creator><creator>Balas, Antonio</creator><creator>Rudilla, Francesc</creator><creator>Barea, Luisa</creator><creator>Castro, Ana</creator><creator>Larrea, Luis</creator><creator>Perez-Vaquero, Miguel A.</creator><creator>Prat, Isidro</creator><creator>Querol, Sergio</creator><creator>Garrido, Gregorio</creator><creator>Matesanz, Rafael</creator><creator>Carreras, Enric</creator><creator>Duarte, Rafael F.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201806</creationdate><title>Analysis of the Spanish CCR5-∆32 inventory of cord blood units: lower cell counts in homozygous donors</title><author>Enrich, Emma ; Vidal, Francisco ; Sánchez-Gordo, Francisco ; Gómez-Zumaquero, Juan M. ; Balas, Antonio ; Rudilla, Francesc ; Barea, Luisa ; Castro, Ana ; Larrea, Luis ; Perez-Vaquero, Miguel A. ; Prat, Isidro ; Querol, Sergio ; Garrido, Gregorio ; Matesanz, Rafael ; Carreras, Enric ; Duarte, Rafael F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3198-466d0cb5356ed782007c77b11d5e2424be34bee6697c861197e625019229bf113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>45</topic><topic>45/77</topic><topic>631/250/1904</topic><topic>692/308/2171</topic><topic>Analysis</topic><topic>Blood groups</topic><topic>Care and treatment</topic><topic>CCR5 protein</topic><topic>CD34 antigen</topic><topic>Cell Biology</topic><topic>Chemokine receptors</topic><topic>Clinical trials</topic><topic>Clonal deletion</topic><topic>Cord blood</topic><topic>Cord Blood Stem Cell Transplantation - methods</topic><topic>Female</topic><topic>Fetal blood</topic><topic>Gene deletion</topic><topic>Genotype</topic><topic>Hematology</topic><topic>Histocompatibility antigen HLA</topic><topic>HIV</topic><topic>HIV infections</topic><topic>HIV patients</topic><topic>Homozygote</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Infection</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mutation</topic><topic>Patients</topic><topic>Public Health</topic><topic>Receptors, CCR5 - immunology</topic><topic>Stem cell transplantation</topic><topic>Stem Cells</topic><topic>Surgery</topic><topic>Tissue Donors</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><topic>Umbilical cord</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Enrich, Emma</creatorcontrib><creatorcontrib>Vidal, Francisco</creatorcontrib><creatorcontrib>Sánchez-Gordo, Francisco</creatorcontrib><creatorcontrib>Gómez-Zumaquero, Juan M.</creatorcontrib><creatorcontrib>Balas, Antonio</creatorcontrib><creatorcontrib>Rudilla, Francesc</creatorcontrib><creatorcontrib>Barea, Luisa</creatorcontrib><creatorcontrib>Castro, Ana</creatorcontrib><creatorcontrib>Larrea, Luis</creatorcontrib><creatorcontrib>Perez-Vaquero, Miguel A.</creatorcontrib><creatorcontrib>Prat, Isidro</creatorcontrib><creatorcontrib>Querol, Sergio</creatorcontrib><creatorcontrib>Garrido, Gregorio</creatorcontrib><creatorcontrib>Matesanz, Rafael</creatorcontrib><creatorcontrib>Carreras, Enric</creatorcontrib><creatorcontrib>Duarte, Rafael F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Bone marrow transplantation (Basingstoke)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Enrich, Emma</au><au>Vidal, Francisco</au><au>Sánchez-Gordo, Francisco</au><au>Gómez-Zumaquero, Juan M.</au><au>Balas, Antonio</au><au>Rudilla, Francesc</au><au>Barea, Luisa</au><au>Castro, Ana</au><au>Larrea, Luis</au><au>Perez-Vaquero, Miguel A.</au><au>Prat, Isidro</au><au>Querol, Sergio</au><au>Garrido, Gregorio</au><au>Matesanz, Rafael</au><au>Carreras, Enric</au><au>Duarte, Rafael F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the Spanish CCR5-∆32 inventory of cord blood units: lower cell counts in homozygous donors</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><stitle>Bone Marrow Transplant</stitle><addtitle>Bone Marrow Transplant</addtitle><date>2018-06</date><risdate>2018</risdate><volume>53</volume><issue>6</issue><spage>741</spage><epage>748</epage><pages>741-748</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><abstract>The possibility to use
CCR5
-∆32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the
CCR5
-∆32 mutation. To achieve an inventory of
CCR5
-∆32 cord blood units, the 20,236 best cell quality units of the Spanish Registry were genotyped. Furthermore, their CD34
+
and total nucleated cells counts, blood type, gender, HLA and donor’s geographical and ancestral origin were analyzed. The results showed 130 (0.64%) units homozygous for the deletion, 2,646 (13.08%) heterozygous and 17,460 (86.28%) did not present the mutation. Interestingly, a significant lower amount of CD34
+
cells was found in the
CCR5
-∆32 homozygous units. In addition, a significant association was found among donor’s ancestral origin and the mutation, with a higher percentage of CCR5-∆32 units with a European ancestry. In summary, identification of a relatively high number of
CCR5
-∆32 units is feasible and will facilitate the development of clinical trials for HIV cure in patients requiring hematopoietic transplantation. Further studies are required to understand the significance of lower cell counts within the
CCR5
-∆32 homozygous group and its clinical impact.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29410548</pmid><doi>10.1038/s41409-018-0114-8</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-3369 |
| ispartof | Bone marrow transplantation (Basingstoke), 2018-06, Vol.53 (6), p.741-748 |
| issn | 0268-3369 1476-5365 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1999192358 |
| source | MEDLINE; Springer Journals; Springer Nature - Connect here FIRST to enable access; EZB Electronic Journals Library |
| subjects | 45 45/77 631/250/1904 692/308/2171 Analysis Blood groups Care and treatment CCR5 protein CD34 antigen Cell Biology Chemokine receptors Clinical trials Clonal deletion Cord blood Cord Blood Stem Cell Transplantation - methods Female Fetal blood Gene deletion Genotype Hematology Histocompatibility antigen HLA HIV HIV infections HIV patients Homozygote Human immunodeficiency virus Humans Infection Internal Medicine Male Medical research Medicine Medicine & Public Health Mutation Patients Public Health Receptors, CCR5 - immunology Stem cell transplantation Stem Cells Surgery Tissue Donors Transplantation Transplants & implants Umbilical cord |
| title | Analysis of the Spanish CCR5-∆32 inventory of cord blood units: lower cell counts in homozygous donors |
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