3D confocal Raman imaging of oil-rich emulsion from enzyme-assisted aqueous extraction of extruded soybean powder

•The morphology of oil-rich emulsion was visualized by 3D confocal Raman.•The molecular weight of the emulsion protein was decreased to below 30 kDa.•The decreased stability of the emulsion was due to the protein aggregations.•Proteins were aggregated via SS bonds or protein-lipid interactions. The...

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