Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis
Eight Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize Salmonella enterica serovar Enteritidis ( Salmonella enteritidis) interaction with epithelial cells. L. kefir surface properties such as autoaggregation and coaggregation with Salmonell...
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| Veröffentlicht in: | International journal of food microbiology 2007-09, Vol.118 (3), p.264-273 |
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| container_title | International journal of food microbiology |
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| creator | Golowczyc, M.A. Mobili, P. Garrote, G.L. Abraham, A.G. De Antoni, G.L. |
| description | Eight
Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize
Salmonella enterica serovar Enteritidis (
Salmonella enteritidis) interaction with epithelial cells.
L. kefir surface properties such as autoaggregation and coaggregation with
Salmonella and adhesion to Caco-2/TC-7 cells were evaluated.
L. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with
Salmonella.
Coincubation of
Salmonella with coaggregating
L. kefir strains significantly decreased its capacity to adhere to and to invade Caco-2/TC-7 cells. This was not observed with non coaggregating
L. kefir strains.
Spent culture supernatants of
L. kefir contain significant amounts of S-layer proteins.
Salmonella pretreated with spent culture supernatants (pH 4.5–4.7) from all tested
L. kefir strains showed a significant decrease in association and invasion to Caco-2/TC-7 cells. Artificially acidified MRS containing lactic acid to a final concentration and pH equivalent to lactobacilli spent culture supernatants did not show any protective action. Pretreatment of this pathogen with spent culture supernatants reduced microvilli disorganization produced by
Salmonella. In addition,
Salmonella pretreated with S-layer proteins extracted from coaggregating and non coaggregating
L. kefir strains were unable to invade Caco-2/TC-7 cells. After treatment,
L. kefir S-layer protein was detected associated with
Salmonella, suggesting a protective role of this protein on association and invasion. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2007.07.042 |
| format | Article |
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Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize
Salmonella enterica serovar Enteritidis (
Salmonella enteritidis) interaction with epithelial cells.
L. kefir surface properties such as autoaggregation and coaggregation with
Salmonella and adhesion to Caco-2/TC-7 cells were evaluated.
L. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with
Salmonella.
Coincubation of
Salmonella with coaggregating
L. kefir strains significantly decreased its capacity to adhere to and to invade Caco-2/TC-7 cells. This was not observed with non coaggregating
L. kefir strains.
Spent culture supernatants of
L. kefir contain significant amounts of S-layer proteins.
Salmonella pretreated with spent culture supernatants (pH 4.5–4.7) from all tested
L. kefir strains showed a significant decrease in association and invasion to Caco-2/TC-7 cells. Artificially acidified MRS containing lactic acid to a final concentration and pH equivalent to lactobacilli spent culture supernatants did not show any protective action. Pretreatment of this pathogen with spent culture supernatants reduced microvilli disorganization produced by
Salmonella. In addition,
Salmonella pretreated with S-layer proteins extracted from coaggregating and non coaggregating
L. kefir strains were unable to invade Caco-2/TC-7 cells. After treatment,
L. kefir S-layer protein was detected associated with
Salmonella, suggesting a protective role of this protein on association and invasion.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2007.07.042</identifier><identifier>PMID: 17719671</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>antagonists ; antibacterial proteins ; Antibiosis ; autoaggregation ; Bacterial Adhesion - physiology ; bacterial contamination ; bacterial proteins ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Biological and medical sciences ; Caco-2 Cells - microbiology ; coaggregation ; coculture ; Coculture Techniques ; Colony Count, Microbial ; culture filtrates ; food contamination ; Food industries ; Food Microbiology ; food pathogens ; food preservatives ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrogen-Ion Concentration ; isolation ; kefir ; lactic acid bacteria ; Lactobacillus ; Lactobacillus - physiology ; Lactobacillus kefir ; Lactobacillus kefiri ; Membrane Glycoproteins - metabolism ; Membrane Glycoproteins - physiology ; outer membrane proteins ; Probiotic ; Probiotics ; S-layer ; Salmonella enterica ; Salmonella enteritidis ; Salmonella enteritidis - physiology ; Salmonella sp</subject><ispartof>International journal of food microbiology, 2007-09, Vol.118 (3), p.264-273</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c567t-b45cc2c0d44388b739f0d3b6cc12f8442927b82eb2542ee99eeef3ad2eba18343</citedby><cites>FETCH-LOGICAL-c567t-b45cc2c0d44388b739f0d3b6cc12f8442927b82eb2542ee99eeef3ad2eba18343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160507003753$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19102199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17719671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Golowczyc, M.A.</creatorcontrib><creatorcontrib>Mobili, P.</creatorcontrib><creatorcontrib>Garrote, G.L.</creatorcontrib><creatorcontrib>Abraham, A.G.</creatorcontrib><creatorcontrib>De Antoni, G.L.</creatorcontrib><title>Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Eight
Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize
Salmonella enterica serovar Enteritidis (
Salmonella enteritidis) interaction with epithelial cells.
L. kefir surface properties such as autoaggregation and coaggregation with
Salmonella and adhesion to Caco-2/TC-7 cells were evaluated.
L. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with
Salmonella.
Coincubation of
Salmonella with coaggregating
L. kefir strains significantly decreased its capacity to adhere to and to invade Caco-2/TC-7 cells. This was not observed with non coaggregating
L. kefir strains.
Spent culture supernatants of
L. kefir contain significant amounts of S-layer proteins.
Salmonella pretreated with spent culture supernatants (pH 4.5–4.7) from all tested
L. kefir strains showed a significant decrease in association and invasion to Caco-2/TC-7 cells. Artificially acidified MRS containing lactic acid to a final concentration and pH equivalent to lactobacilli spent culture supernatants did not show any protective action. Pretreatment of this pathogen with spent culture supernatants reduced microvilli disorganization produced by
Salmonella. In addition,
Salmonella pretreated with S-layer proteins extracted from coaggregating and non coaggregating
L. kefir strains were unable to invade Caco-2/TC-7 cells. After treatment,
L. kefir S-layer protein was detected associated with
Salmonella, suggesting a protective role of this protein on association and invasion.</description><subject>antagonists</subject><subject>antibacterial proteins</subject><subject>Antibiosis</subject><subject>autoaggregation</subject><subject>Bacterial Adhesion - physiology</subject><subject>bacterial contamination</subject><subject>bacterial proteins</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells - microbiology</subject><subject>coaggregation</subject><subject>coculture</subject><subject>Coculture Techniques</subject><subject>Colony Count, Microbial</subject><subject>culture filtrates</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>food preservatives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>isolation</subject><subject>kefir</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus</subject><subject>Lactobacillus - physiology</subject><subject>Lactobacillus kefir</subject><subject>Lactobacillus kefiri</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Membrane Glycoproteins - physiology</subject><subject>outer membrane proteins</subject><subject>Probiotic</subject><subject>Probiotics</subject><subject>S-layer</subject><subject>Salmonella enterica</subject><subject>Salmonella enteritidis</subject><subject>Salmonella enteritidis - physiology</subject><subject>Salmonella sp</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9rGzEQxUVpaZy0X6FVD-1tXf3bXelYTNoUDC24OYtZ7cjIXa8SaW3wt482NqTHwsCI4fc0T0-EfOJsyRlvvu6WYedj7PfBpbgUjLXLuZR4RRZct6aSqmGvyaKwuuINq6_Idc47xlgtJXtLrnjbctO0fEEef6c4oZvCESmUFkcaPV2XY-zAhWE4ZPoXfUjUQUqnMG7pphrghIk-zMowUthCGPNENzDs44jDABTHCVNwQDOmeIREb58HU-hDfkfeeBgyvr_0G3L__fbP6q5a__rxc_VtXbm6aaeqU7VzwrFeKal110rjWS-7xjkuvFZKGNF2WmAnaiUQjUFEL6EvE-BaKnlDvpzvLT4fD5gnuw_ZzfZGjIdsuTG6rk1TQHMGS5g5J_T2IYU9pJPlzM552539J287523nUqJoP1yWHLo99i_KS8AF-HwBIDsYfILRhfzCGc5EcVK4j2fOQ7SwTYW53wjGJWNa6Pr5PaszgSW0Y8Bksws4OuxDKh9o-xj-w_ATdu2wjA</recordid><startdate>20070930</startdate><enddate>20070930</enddate><creator>Golowczyc, M.A.</creator><creator>Mobili, P.</creator><creator>Garrote, G.L.</creator><creator>Abraham, A.G.</creator><creator>De Antoni, G.L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20070930</creationdate><title>Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis</title><author>Golowczyc, M.A. ; Mobili, P. ; Garrote, G.L. ; Abraham, A.G. ; De Antoni, G.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c567t-b45cc2c0d44388b739f0d3b6cc12f8442927b82eb2542ee99eeef3ad2eba18343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>antagonists</topic><topic>antibacterial proteins</topic><topic>Antibiosis</topic><topic>autoaggregation</topic><topic>Bacterial Adhesion - physiology</topic><topic>bacterial contamination</topic><topic>bacterial proteins</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells - microbiology</topic><topic>coaggregation</topic><topic>coculture</topic><topic>Coculture Techniques</topic><topic>Colony Count, Microbial</topic><topic>culture filtrates</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>food preservatives</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>isolation</topic><topic>kefir</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus</topic><topic>Lactobacillus - physiology</topic><topic>Lactobacillus kefir</topic><topic>Lactobacillus kefiri</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Membrane Glycoproteins - physiology</topic><topic>outer membrane proteins</topic><topic>Probiotic</topic><topic>Probiotics</topic><topic>S-layer</topic><topic>Salmonella enterica</topic><topic>Salmonella enteritidis</topic><topic>Salmonella enteritidis - physiology</topic><topic>Salmonella sp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Golowczyc, M.A.</creatorcontrib><creatorcontrib>Mobili, P.</creatorcontrib><creatorcontrib>Garrote, G.L.</creatorcontrib><creatorcontrib>Abraham, A.G.</creatorcontrib><creatorcontrib>De Antoni, G.L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Golowczyc, M.A.</au><au>Mobili, P.</au><au>Garrote, G.L.</au><au>Abraham, A.G.</au><au>De Antoni, G.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2007-09-30</date><risdate>2007</risdate><volume>118</volume><issue>3</issue><spage>264</spage><epage>273</epage><pages>264-273</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Eight
Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize
Salmonella enterica serovar Enteritidis (
Salmonella enteritidis) interaction with epithelial cells.
L. kefir surface properties such as autoaggregation and coaggregation with
Salmonella and adhesion to Caco-2/TC-7 cells were evaluated.
L. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four strains coaggregated with
Salmonella.
Coincubation of
Salmonella with coaggregating
L. kefir strains significantly decreased its capacity to adhere to and to invade Caco-2/TC-7 cells. This was not observed with non coaggregating
L. kefir strains.
Spent culture supernatants of
L. kefir contain significant amounts of S-layer proteins.
Salmonella pretreated with spent culture supernatants (pH 4.5–4.7) from all tested
L. kefir strains showed a significant decrease in association and invasion to Caco-2/TC-7 cells. Artificially acidified MRS containing lactic acid to a final concentration and pH equivalent to lactobacilli spent culture supernatants did not show any protective action. Pretreatment of this pathogen with spent culture supernatants reduced microvilli disorganization produced by
Salmonella. In addition,
Salmonella pretreated with S-layer proteins extracted from coaggregating and non coaggregating
L. kefir strains were unable to invade Caco-2/TC-7 cells. After treatment,
L. kefir S-layer protein was detected associated with
Salmonella, suggesting a protective role of this protein on association and invasion.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17719671</pmid><doi>10.1016/j.ijfoodmicro.2007.07.042</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | International journal of food microbiology, 2007-09, Vol.118 (3), p.264-273 |
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| language | eng |
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| source | ScienceDirect; MEDLINE |
| subjects | antagonists antibacterial proteins Antibiosis autoaggregation Bacterial Adhesion - physiology bacterial contamination bacterial proteins Bacterial Proteins - metabolism Bacterial Proteins - physiology Biological and medical sciences Caco-2 Cells - microbiology coaggregation coculture Coculture Techniques Colony Count, Microbial culture filtrates food contamination Food industries Food Microbiology food pathogens food preservatives Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration isolation kefir lactic acid bacteria Lactobacillus Lactobacillus - physiology Lactobacillus kefir Lactobacillus kefiri Membrane Glycoproteins - metabolism Membrane Glycoproteins - physiology outer membrane proteins Probiotic Probiotics S-layer Salmonella enterica Salmonella enteritidis Salmonella enteritidis - physiology Salmonella sp |
| title | Protective action of Lactobacillus kefir carrying S-layer protein against Salmonella enterica serovar Enteritidis |
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