Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity
The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605 Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken...
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| Veröffentlicht in: | Water research (Oxford) 2007-02, Vol.41 (4), p.803-809 |
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| creator | Haznedaroglu, Berat Z. Yurtsever, Deniz Lefkowitz, Jamie R. Duran, Metin |
| description | The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605
Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a “signature” FAME, a FAME that is uniquely associated with a particular host category. However, two-sample
t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates. |
| doi_str_mv | 10.1016/j.watres.2006.11.041 |
| format | Article |
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t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2006.11.041</identifier><identifier>PMID: 17234236</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>analytical methods ; animal manures ; Animals ; Animals, Domestic ; Animals, Wild ; Applied sciences ; Deer ; discriminant analysis ; enteropathogens ; Environmental Monitoring ; Escherichia coli ; Escherichia coli - chemistry ; Escherichia coli - classification ; Escherichia coli - isolation & purification ; Exact sciences and technology ; FAME ; fatty acid methyl ester (FAME) profiles ; Fatty Acids - analysis ; Fecal pollution ; feces ; Feces - microbiology ; host specificity ; Humans ; Microbial source tracking ; Other industrial wastes. Sewage sludge ; Phenotype ; Pollution ; Poultry ; Sensitivity and Specificity ; sewage ; TMDL ; Wastes ; Water Microbiology ; Water Pollutants ; water pollution ; Water treatment and pollution</subject><ispartof>Water research (Oxford), 2007-02, Vol.41 (4), p.803-809</ispartof><rights>2006 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-3918784350b0fd366c399de05fbab66abc300e1871192d682ce9770a0a2fcfb53</citedby><cites>FETCH-LOGICAL-c445t-3918784350b0fd366c399de05fbab66abc300e1871192d682ce9770a0a2fcfb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.watres.2006.11.041$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18497440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17234236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haznedaroglu, Berat Z.</creatorcontrib><creatorcontrib>Yurtsever, Deniz</creatorcontrib><creatorcontrib>Lefkowitz, Jamie R.</creatorcontrib><creatorcontrib>Duran, Metin</creatorcontrib><title>Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605
Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a “signature” FAME, a FAME that is uniquely associated with a particular host category. However, two-sample
t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.</description><subject>analytical methods</subject><subject>animal manures</subject><subject>Animals</subject><subject>Animals, Domestic</subject><subject>Animals, Wild</subject><subject>Applied sciences</subject><subject>Deer</subject><subject>discriminant analysis</subject><subject>enteropathogens</subject><subject>Environmental Monitoring</subject><subject>Escherichia coli</subject><subject>Escherichia coli - chemistry</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - isolation & purification</subject><subject>Exact sciences and technology</subject><subject>FAME</subject><subject>fatty acid methyl ester (FAME) profiles</subject><subject>Fatty Acids - analysis</subject><subject>Fecal pollution</subject><subject>feces</subject><subject>Feces - microbiology</subject><subject>host specificity</subject><subject>Humans</subject><subject>Microbial source tracking</subject><subject>Other industrial wastes. Sewage sludge</subject><subject>Phenotype</subject><subject>Pollution</subject><subject>Poultry</subject><subject>Sensitivity and Specificity</subject><subject>sewage</subject><subject>TMDL</subject><subject>Wastes</subject><subject>Water Microbiology</subject><subject>Water Pollutants</subject><subject>water pollution</subject><subject>Water treatment and pollution</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo7uzoPxDNRW_dVj766yIsy7oKCwq655CuTqYz9HTGJLPD-OvN0AN781RQPPXy1kPIOwYlA1Z_3pZHnYKJJQeoS8ZKkOwFWbG26QouZfuSrACkKJio5BW5jnELAJyL7jW5Yg0Xkot6RY4_RzP7dNo7pDjqoDGZ4P7q5PxMvaV3Ece8wNFpin5yNI3BHzYjPY5-MgWaaaJWp3SiGt1A98FbN7l5Q5Onbn4yMbmNToaOPiYa9waddejS6Q15ZfUUzdvLXJPHr3e_b78VDz_uv9_ePBQoZZUK0eV_Wikq6MEOoq5RdN1goLK97uta9ygATGYY6_hQtxxN1zSgQXOLtq_EmnxacnOzP4dcR-1cPLfWs_GHqFjXtZJlT2siFxCDjzEYq_bB7XQ4KQbqLFxt1SJcnYUrxlQWns_eX_IP_c4Mz0cXwxn4eAF0RD3ZoGd08ZlrZddICZn7sHBWe6U3ITOPvziw_F9TgYBz0peFMNnXkzNBRXRmRjO4YDCpwbv_d_0HFFGsOg</recordid><startdate>20070201</startdate><enddate>20070201</enddate><creator>Haznedaroglu, Berat Z.</creator><creator>Yurtsever, Deniz</creator><creator>Lefkowitz, Jamie R.</creator><creator>Duran, Metin</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7T7</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H96</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20070201</creationdate><title>Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity</title><author>Haznedaroglu, Berat Z. ; Yurtsever, Deniz ; Lefkowitz, Jamie R. ; Duran, Metin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-3918784350b0fd366c399de05fbab66abc300e1871192d682ce9770a0a2fcfb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>analytical methods</topic><topic>animal manures</topic><topic>Animals</topic><topic>Animals, Domestic</topic><topic>Animals, Wild</topic><topic>Applied sciences</topic><topic>Deer</topic><topic>discriminant analysis</topic><topic>enteropathogens</topic><topic>Environmental Monitoring</topic><topic>Escherichia coli</topic><topic>Escherichia coli - chemistry</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - isolation & purification</topic><topic>Exact sciences and technology</topic><topic>FAME</topic><topic>fatty acid methyl ester (FAME) profiles</topic><topic>Fatty Acids - analysis</topic><topic>Fecal pollution</topic><topic>feces</topic><topic>Feces - microbiology</topic><topic>host specificity</topic><topic>Humans</topic><topic>Microbial source tracking</topic><topic>Other industrial wastes. Sewage sludge</topic><topic>Phenotype</topic><topic>Pollution</topic><topic>Poultry</topic><topic>Sensitivity and Specificity</topic><topic>sewage</topic><topic>TMDL</topic><topic>Wastes</topic><topic>Water Microbiology</topic><topic>Water Pollutants</topic><topic>water pollution</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haznedaroglu, Berat Z.</creatorcontrib><creatorcontrib>Yurtsever, Deniz</creatorcontrib><creatorcontrib>Lefkowitz, Jamie R.</creatorcontrib><creatorcontrib>Duran, Metin</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haznedaroglu, Berat Z.</au><au>Yurtsever, Deniz</au><au>Lefkowitz, Jamie R.</au><au>Duran, Metin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity</atitle><jtitle>Water research (Oxford)</jtitle><addtitle>Water Res</addtitle><date>2007-02-01</date><risdate>2007</risdate><volume>41</volume><issue>4</issue><spage>803</spage><epage>809</epage><pages>803-809</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>The objective of the study was to investigate whole-cell fatty acid methyl ester (FAME) profiles of 605
Escherichia coli isolates to determine their host specificity. The isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. The FAME profiles were presented as the relative masses of 12 FAMEs identified in the isolates and it was found that none of the six hosts carried a “signature” FAME, a FAME that is uniquely associated with a particular host category. However, two-sample
t-test analyses indicated that the mean relative masses of seven FAMEs out of the 12 identified showed statistically significant differences (95% confidence interval) between isolates of human and non-human origins. In addition, a linear discriminant function based on mean relative mass variations in individual FAMEs classified the known-source isolates into their respective host categories with a 47.6% average rate of correct classification (ARCC) in a six-way discriminant analysis. The ARCC increased to 61.3% when the individual hosts were pooled into larger categories of human, livestock, and wildlife. The accuracy was 75.5% when isolates of human origin were discriminated against those of non-human origins. Random cluster formation analysis indicated that the library size was sufficient to prevent random grouping among the isolates.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>17234236</pmid><doi>10.1016/j.watres.2006.11.041</doi><tpages>7</tpages></addata></record> |
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| ispartof | Water research (Oxford), 2007-02, Vol.41 (4), p.803-809 |
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| subjects | analytical methods animal manures Animals Animals, Domestic Animals, Wild Applied sciences Deer discriminant analysis enteropathogens Environmental Monitoring Escherichia coli Escherichia coli - chemistry Escherichia coli - classification Escherichia coli - isolation & purification Exact sciences and technology FAME fatty acid methyl ester (FAME) profiles Fatty Acids - analysis Fecal pollution feces Feces - microbiology host specificity Humans Microbial source tracking Other industrial wastes. Sewage sludge Phenotype Pollution Poultry Sensitivity and Specificity sewage TMDL Wastes Water Microbiology Water Pollutants water pollution Water treatment and pollution |
| title | Phenotypic characterization of Escherichia coli through whole-cell fatty acid profiling to investigate host specificity |
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