ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle
Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression...
Gespeichert in:
| Veröffentlicht in: | International journal of experimental pathology 2006-12, Vol.87 (6), p.437-443 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 443 |
|---|---|
| container_issue | 6 |
| container_start_page | 437 |
| container_title | International journal of experimental pathology |
| container_volume | 87 |
| creator | Carvalho, Robson Francisco Dariolli, Rafael Justulin Junior, Luis Antonio Sugizaki, Mario Mateus Politi Okoshi, Marina Cicogna, Antonio Carlos Felisbino, Sergio Luis Dal Pai-Silva, Maeli |
| description | Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure. |
| doi_str_mv | 10.1111/j.1365-2613.2006.00497.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_19981532</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19981532</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_199815323</originalsourceid><addsrcrecordid>eNqNjMFOwzAQRK0KpAboP-yJW4wdkwRzq6pCI1UgVb1Xq7CtXByneB0U_p4iIc7MZQ5v3ggBWkl9zt1RalOVeVFpIwulKqnUva3lOBHZH7gQmbKlzW1Vm6m4Yj4qpU2h60zw66Z5bl7ma5hvts1ivXyEFWFMsEfnh0iAPlFk6DBFN0JHCb3vT7FP5AIywYECAY2nSMyuD4DhDbBN7tOlL3ABIibgd_I_InQDt55uxOUePdPst6_F7dNyu1jl59uPgTjtOscteY-B-oF32toHXZrC_Hv4DXt9VrE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19981532</pqid></control><display><type>article</type><title>ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle</title><source>Access via Wiley Online Library</source><source>PubMed Central</source><creator>Carvalho, Robson Francisco ; Dariolli, Rafael ; Justulin Junior, Luis Antonio ; Sugizaki, Mario Mateus ; Politi Okoshi, Marina ; Cicogna, Antonio Carlos ; Felisbino, Sergio Luis ; Dal Pai-Silva, Maeli</creator><creatorcontrib>Carvalho, Robson Francisco ; Dariolli, Rafael ; Justulin Junior, Luis Antonio ; Sugizaki, Mario Mateus ; Politi Okoshi, Marina ; Cicogna, Antonio Carlos ; Felisbino, Sergio Luis ; Dal Pai-Silva, Maeli</creatorcontrib><description>Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.</description><identifier>ISSN: 0959-9673</identifier><identifier>EISSN: 1365-2613</identifier><identifier>DOI: 10.1111/j.1365-2613.2006.00497.x</identifier><language>eng</language><ispartof>International journal of experimental pathology, 2006-12, Vol.87 (6), p.437-443</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Carvalho, Robson Francisco</creatorcontrib><creatorcontrib>Dariolli, Rafael</creatorcontrib><creatorcontrib>Justulin Junior, Luis Antonio</creatorcontrib><creatorcontrib>Sugizaki, Mario Mateus</creatorcontrib><creatorcontrib>Politi Okoshi, Marina</creatorcontrib><creatorcontrib>Cicogna, Antonio Carlos</creatorcontrib><creatorcontrib>Felisbino, Sergio Luis</creatorcontrib><creatorcontrib>Dal Pai-Silva, Maeli</creatorcontrib><title>ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle</title><title>International journal of experimental pathology</title><description>Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.</description><issn>0959-9673</issn><issn>1365-2613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNjMFOwzAQRK0KpAboP-yJW4wdkwRzq6pCI1UgVb1Xq7CtXByneB0U_p4iIc7MZQ5v3ggBWkl9zt1RalOVeVFpIwulKqnUva3lOBHZH7gQmbKlzW1Vm6m4Yj4qpU2h60zw66Z5bl7ma5hvts1ivXyEFWFMsEfnh0iAPlFk6DBFN0JHCb3vT7FP5AIywYECAY2nSMyuD4DhDbBN7tOlL3ABIibgd_I_InQDt55uxOUePdPst6_F7dNyu1jl59uPgTjtOscteY-B-oF32toHXZrC_Hv4DXt9VrE</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Carvalho, Robson Francisco</creator><creator>Dariolli, Rafael</creator><creator>Justulin Junior, Luis Antonio</creator><creator>Sugizaki, Mario Mateus</creator><creator>Politi Okoshi, Marina</creator><creator>Cicogna, Antonio Carlos</creator><creator>Felisbino, Sergio Luis</creator><creator>Dal Pai-Silva, Maeli</creator><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20061201</creationdate><title>ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle</title><author>Carvalho, Robson Francisco ; Dariolli, Rafael ; Justulin Junior, Luis Antonio ; Sugizaki, Mario Mateus ; Politi Okoshi, Marina ; Cicogna, Antonio Carlos ; Felisbino, Sergio Luis ; Dal Pai-Silva, Maeli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_199815323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carvalho, Robson Francisco</creatorcontrib><creatorcontrib>Dariolli, Rafael</creatorcontrib><creatorcontrib>Justulin Junior, Luis Antonio</creatorcontrib><creatorcontrib>Sugizaki, Mario Mateus</creatorcontrib><creatorcontrib>Politi Okoshi, Marina</creatorcontrib><creatorcontrib>Cicogna, Antonio Carlos</creatorcontrib><creatorcontrib>Felisbino, Sergio Luis</creatorcontrib><creatorcontrib>Dal Pai-Silva, Maeli</creatorcontrib><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of experimental pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carvalho, Robson Francisco</au><au>Dariolli, Rafael</au><au>Justulin Junior, Luis Antonio</au><au>Sugizaki, Mario Mateus</au><au>Politi Okoshi, Marina</au><au>Cicogna, Antonio Carlos</au><au>Felisbino, Sergio Luis</au><au>Dal Pai-Silva, Maeli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle</atitle><jtitle>International journal of experimental pathology</jtitle><date>2006-12-01</date><risdate>2006</risdate><volume>87</volume><issue>6</issue><spage>437</spage><epage>443</epage><pages>437-443</pages><issn>0959-9673</issn><eissn>1365-2613</eissn><abstract>Heart failure is associated with a skeletal muscle myopathy with cellular and extracellular alterations. The hypothesis of this investigation is that extracellular changes may be associated with enhanced mRNA expression and activity of matrix metalloproteinases (MMP). We examined MMP mRNA expression and MMP activity in Soleus (SOL), extensor digitorum longus (EDL), and diaphragm (DIA) muscles of young Wistar rat with monocrotaline-induced heart failure. Rats injected with saline served as age-matched controls. MMP2 and MMP9 mRNA contents were determined by RT-PCR and MMP activity by electrophoresis in gelatin-containing polyacrylamide gels in the presence of SDS under non-reducing conditions. Heart failure increased MMP9 mRNA expression and activity in SOL, EDL and DIA and MMP2 mRNA expression in DIA. These results suggest that MMP changes may contribute to the skeletal muscle myopathy during heart failure.</abstract><doi>10.1111/j.1365-2613.2006.00497.x</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-9673 |
| ispartof | International journal of experimental pathology, 2006-12, Vol.87 (6), p.437-443 |
| issn | 0959-9673 1365-2613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19981532 |
| source | Access via Wiley Online Library; PubMed Central |
| title | ORIGINAL ARTICLE: Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T16%3A32%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ORIGINAL%20ARTICLE:%20Heart%20failure%20alters%20matrix%20metalloproteinase%20gene%20expression%20and%20activity%20in%20rat%20skeletal%20muscle&rft.jtitle=International%20journal%20of%20experimental%20pathology&rft.au=Carvalho,%20Robson%20Francisco&rft.date=2006-12-01&rft.volume=87&rft.issue=6&rft.spage=437&rft.epage=443&rft.pages=437-443&rft.issn=0959-9673&rft.eissn=1365-2613&rft_id=info:doi/10.1111/j.1365-2613.2006.00497.x&rft_dat=%3Cproquest%3E19981532%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19981532&rft_id=info:pmid/&rfr_iscdi=true |