Utilization of a polyphasic approach in the taxonomic reassessment of antibiotic- and enzyme-producing Bacillus spp. isolated from the Philippines
The taxonomy of 58 locally isolated antibiotic- and enzyme-producing Bacillus isolates deposited at the Philippine National Collection of Microorganisms (PNCM)-BIOTECH was reassessed in this study using a polyphasic approach since they had been only partially identified prior to deposition in the cu...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2005-07, Vol.21 (5), p.635-644 |
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| container_title | World journal of microbiology & biotechnology |
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| creator | GUERRA-CANTERA, Marie Antonette Ruth V RAYMUNDO, Asuncion K |
| description | The taxonomy of 58 locally isolated antibiotic- and enzyme-producing Bacillus isolates deposited at the Philippine National Collection of Microorganisms (PNCM)-BIOTECH was reassessed in this study using a polyphasic approach since they had been only partially identified prior to deposition in the culture collection. The isolates had 41.1-69% G + C, and possessed the characteristic diaminopimelic acid (DAP) and fatty acid methyl ester (FAMEs) properties of Bacillus species. Molecular analysis using specific PCR primers differentiated the isolates into two major groups, the Bacillus cereus group and the Bacillus subtilis group. To further differentiate these isolates, they were subjected to 39 phenotypic tests. Using the dichotomous key constructed for Bacillus, 45 isolates maintained their original identities, five were named at the species level, and 12 were re-identified and renamed. These results showed that the classical phenotypic tests allowed the reclassification of the isolates, while modern techniques of chemotaxonomy and the molecular approach led only to genus and cluster classification and confirmation.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s11274-004-3567-4 |
| format | Article |
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The isolates had 41.1-69% G + C, and possessed the characteristic diaminopimelic acid (DAP) and fatty acid methyl ester (FAMEs) properties of Bacillus species. Molecular analysis using specific PCR primers differentiated the isolates into two major groups, the Bacillus cereus group and the Bacillus subtilis group. To further differentiate these isolates, they were subjected to 39 phenotypic tests. Using the dichotomous key constructed for Bacillus, 45 isolates maintained their original identities, five were named at the species level, and 12 were re-identified and renamed. 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| ispartof | World journal of microbiology & biotechnology, 2005-07, Vol.21 (5), p.635-644 |
| issn | 0959-3993 1573-0972 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Antibiotics Bacillus Bacillus cereus Bacillus subtilis Bacteria Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Chemotaxonomy Enzymes Fundamental and applied biological sciences. Psychology Microbiology Microorganisms Miscellaneous Mission oriented research |
| title | Utilization of a polyphasic approach in the taxonomic reassessment of antibiotic- and enzyme-producing Bacillus spp. isolated from the Philippines |
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