Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease
Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence i...
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| Veröffentlicht in: | Annals of the Entomological Society of America 2006-07, Vol.99 (4), p.707-713, Article 707 |
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| creator | Brelsfoard, C. L Fritz, G. N Rodriguez, R |
| description | Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia. |
| doi_str_mv | 10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2 |
| format | Article |
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Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.</description><subject>Albitarsis Complex</subject><subject>Albitarsis species complex</subject><subject>Anopheles</subject><subject>Anopheles marajoara</subject><subject>cryptic species</subject><subject>Culicidae</subject><subject>Diptera</subject><subject>distribution</subject><subject>GENETICS</subject><subject>geographical distribution</subject><subject>insect taxonomy</subject><subject>insect vectors</subject><subject>internal transcribed spacer 2</subject><subject>internal transcribed spacers</subject><subject>ITS2</subject><subject>molecular sequence data</subject><subject>molecular systematics</subject><subject>new geographic records</subject><subject>Nyssorhynchus</subject><subject>pest identification</subject><subject>Plasmodium</subject><subject>polymerase chain reaction</subject><subject>restriction endonucleases</subject><subject>sequence analysis</subject><subject>taxonomy</subject><issn>0013-8746</issn><issn>1938-2901</issn><issn>0013-8746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqdkdFu0zAUhiMEEmXwDPgKtRcpx3aSxuOGLitQMVYE9GqarFPHWT2ldogTpL4HD4yzIJgQN3Bjy_Z_vnP8_1EUU5jTDPhLAMrjfJFkUwaQzYS4WsDidL1ZXn44v2ZzmBebV-xBNKGC5zETQB9Gk181j6Mn3t8CQMI5m0Tf16W2namMws44S1xFltY1e11rT6aXR-9duz9ate_9jBywxVsXFjI9N02nWzwlRV8bZUrUM2IsOXO1-WaQbL2xN-Sjq4-HoPKaFHsMz580qrs2aEuC4ei71ow3K1s626taB_XT6FGFtdfPfu4n0fbN6kvxLr7YvF0Xy4t4lzLoYoW7hCHjeYYJQJkyjTRNkSY816mieVYhFSplnDEtsqSkOeV5ni1EBjuleMpPohcjt2nd1z4MIw_GK13XaLXrvaRCLBjLWBCuRqFqnfetrmTTmuDGUVKQQyZy8FcO_sohEymEDJnIMRPJJMhiIwfO-z84ynR3xnctmvqfaa9Hmuubvw4U30dc_UZc30M8HxEVOok3rfFy-5mFUqAgqKCDohgVO-Oc1f_58R9yVssS</recordid><startdate>20060701</startdate><enddate>20060701</enddate><creator>Brelsfoard, C. L</creator><creator>Fritz, G. N</creator><creator>Rodriguez, R</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7SS</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20060701</creationdate><title>Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease</title><author>Brelsfoard, C. L ; Fritz, G. N ; Rodriguez, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b520t-cab42a2386a400d52ea155a1438e5c186fa19c52322e964d18138867960bcc353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Albitarsis Complex</topic><topic>Albitarsis species complex</topic><topic>Anopheles</topic><topic>Anopheles marajoara</topic><topic>cryptic species</topic><topic>Culicidae</topic><topic>Diptera</topic><topic>distribution</topic><topic>GENETICS</topic><topic>geographical distribution</topic><topic>insect taxonomy</topic><topic>insect vectors</topic><topic>internal transcribed spacer 2</topic><topic>internal transcribed spacers</topic><topic>ITS2</topic><topic>molecular sequence data</topic><topic>molecular systematics</topic><topic>new geographic records</topic><topic>Nyssorhynchus</topic><topic>pest identification</topic><topic>Plasmodium</topic><topic>polymerase chain reaction</topic><topic>restriction endonucleases</topic><topic>sequence analysis</topic><topic>taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brelsfoard, C. L</creatorcontrib><creatorcontrib>Fritz, G. N</creatorcontrib><creatorcontrib>Rodriguez, R</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Annals of the Entomological Society of America</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brelsfoard, C. L</au><au>Fritz, G. 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We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.</abstract><cop>Oxford, UK</cop><pub>Oxford University Press</pub><doi>10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0013-8746 |
| ispartof | Annals of the Entomological Society of America, 2006-07, Vol.99 (4), p.707-713, Article 707 |
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| language | eng |
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| source | BioOne Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Albitarsis Complex Albitarsis species complex Anopheles Anopheles marajoara cryptic species Culicidae Diptera distribution GENETICS geographical distribution insect taxonomy insect vectors internal transcribed spacer 2 internal transcribed spacers ITS2 molecular sequence data molecular systematics new geographic records Nyssorhynchus pest identification Plasmodium polymerase chain reaction restriction endonucleases sequence analysis taxonomy |
| title | Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease |
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