hemolysin from the mushroom Pleurotus eryngii
A monomeric 17-kDa hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom Pleurotus eryngii, using a protocol that involved gel filtration on Superdex 75, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. Its N-terminal sequence demonstrat...
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| creator | Ngai, Patrick H. K Ng, T. B |
| description | A monomeric 17-kDa hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom Pleurotus eryngii, using a protocol that involved gel filtration on Superdex 75, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. Its N-terminal sequence demonstrated striking homology to that of its counterparts ostreolysin from the oyster mushroom Pleurotus ostreatus and aegerolysin from the mushroom Agrocybe cylindracea. Its hemolytic activity was unaffected over the pH range 4.0-12.0, but no activity was observed at pH 13 and at and below pH 2. The hemolysin was stable between 0 and 30 °C. At 40 °C, only residual activity was detectable. At and above 50 °C, activity was indiscernible. Eryngeolysin exhibited cytotoxicity toward leukemia (L1210) cells but not toward fungi. The hemolysin was inactivated by treatment with trypsin. It exhibited antibacterial activity against Bacillus sp. but not against other species. It inhibited basal as well as ConA-stimulated mitogenic response of murine splenocytes. N-Glycolyneuraminic acid was the only sugar capable of inhibiting the hemolytic activity. Eryngeolysin-induced hemolysis was osmotically protected by polyethylene glycol (PEG) 10000 with a mean hydrated diameter dose to 9.3 nm. However, no protection was offered by PEG 10000 to the anti-mitogenic and antiproliferative activities of eryngeolysin. The susceptibility of erythrocytes from different classes of vertebrates to eryngeolysin was mammalian > avian > reptilian > piscine. |
| doi_str_mv | 10.1007/s00253-006-0406-6 |
| format | Article |
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K ; Ng, T. B</creator><creatorcontrib>Ngai, Patrick H. K ; Ng, T. B</creatorcontrib><description>A monomeric 17-kDa hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom Pleurotus eryngii, using a protocol that involved gel filtration on Superdex 75, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. Its N-terminal sequence demonstrated striking homology to that of its counterparts ostreolysin from the oyster mushroom Pleurotus ostreatus and aegerolysin from the mushroom Agrocybe cylindracea. Its hemolytic activity was unaffected over the pH range 4.0-12.0, but no activity was observed at pH 13 and at and below pH 2. The hemolysin was stable between 0 and 30 °C. At 40 °C, only residual activity was detectable. At and above 50 °C, activity was indiscernible. Eryngeolysin exhibited cytotoxicity toward leukemia (L1210) cells but not toward fungi. The hemolysin was inactivated by treatment with trypsin. It exhibited antibacterial activity against Bacillus sp. but not against other species. It inhibited basal as well as ConA-stimulated mitogenic response of murine splenocytes. N-Glycolyneuraminic acid was the only sugar capable of inhibiting the hemolytic activity. Eryngeolysin-induced hemolysis was osmotically protected by polyethylene glycol (PEG) 10000 with a mean hydrated diameter dose to 9.3 nm. However, no protection was offered by PEG 10000 to the anti-mitogenic and antiproliferative activities of eryngeolysin. The susceptibility of erythrocytes from different classes of vertebrates to eryngeolysin was mammalian > avian > reptilian > piscine.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-006-0406-6</identifier><identifier>PMID: 16804695</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Bacillus ; Biological and medical sciences ; Biotechnology ; Cell Proliferation - drug effects ; Chromatography, Gel ; Chromatography, Ion Exchange ; Cytotoxicity ; Electrophoresis, Polyacrylamide Gel ; Erythrocytes - drug effects ; Filtration ; Fruiting Bodies, Fungal - chemistry ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - chemistry ; Fungi - drug effects ; Hemolysin Proteins - chemistry ; Hemolysin Proteins - isolation & purification ; Hemolysin Proteins - pharmacology ; Hemolysis ; Hemolytic Agents - chemistry ; Hemolytic Agents - isolation & purification ; Hemolytic Agents - pharmacology ; Hydrogen-Ion Concentration ; Leukemia ; Mitosis - drug effects ; Molecular Sequence Data ; Molecular Weight ; Mushrooms ; Neuraminic Acids - metabolism ; Pleurotus - chemistry ; Pleurotus eryngii ; Pleurotus ostreatus ; Polyethylene glycol ; Sequence Analysis, Protein ; Sequence Homology, Amino Acid ; Temperature ; Trypsin - metabolism</subject><ispartof>Applied microbiology and biotechnology, 2006-10, Vol.72 (6), p.1185-1191</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-20730170f50daa1b07d66548d6f75df7ca44c010a9b8ff521ef1f5a78e9135fb3</citedby><cites>FETCH-LOGICAL-c477t-20730170f50daa1b07d66548d6f75df7ca44c010a9b8ff521ef1f5a78e9135fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18239688$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16804695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ngai, Patrick H. K</creatorcontrib><creatorcontrib>Ng, T. B</creatorcontrib><title>hemolysin from the mushroom Pleurotus eryngii</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>A monomeric 17-kDa hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom Pleurotus eryngii, using a protocol that involved gel filtration on Superdex 75, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. Its N-terminal sequence demonstrated striking homology to that of its counterparts ostreolysin from the oyster mushroom Pleurotus ostreatus and aegerolysin from the mushroom Agrocybe cylindracea. Its hemolytic activity was unaffected over the pH range 4.0-12.0, but no activity was observed at pH 13 and at and below pH 2. The hemolysin was stable between 0 and 30 °C. At 40 °C, only residual activity was detectable. At and above 50 °C, activity was indiscernible. Eryngeolysin exhibited cytotoxicity toward leukemia (L1210) cells but not toward fungi. The hemolysin was inactivated by treatment with trypsin. It exhibited antibacterial activity against Bacillus sp. but not against other species. It inhibited basal as well as ConA-stimulated mitogenic response of murine splenocytes. N-Glycolyneuraminic acid was the only sugar capable of inhibiting the hemolytic activity. Eryngeolysin-induced hemolysis was osmotically protected by polyethylene glycol (PEG) 10000 with a mean hydrated diameter dose to 9.3 nm. However, no protection was offered by PEG 10000 to the anti-mitogenic and antiproliferative activities of eryngeolysin. The susceptibility of erythrocytes from different classes of vertebrates to eryngeolysin was mammalian > avian > reptilian > piscine.</description><subject>Bacillus</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Proliferation - drug effects</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Cytotoxicity</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Erythrocytes - drug effects</subject><subject>Filtration</subject><subject>Fruiting Bodies, Fungal - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungi - drug effects</subject><subject>Hemolysin Proteins - chemistry</subject><subject>Hemolysin Proteins - isolation & purification</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>Hemolysis</subject><subject>Hemolytic Agents - chemistry</subject><subject>Hemolytic Agents - isolation & purification</subject><subject>Hemolytic Agents - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Leukemia</subject><subject>Mitosis - drug effects</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Mushrooms</subject><subject>Neuraminic Acids - metabolism</subject><subject>Pleurotus - chemistry</subject><subject>Pleurotus eryngii</subject><subject>Pleurotus ostreatus</subject><subject>Polyethylene glycol</subject><subject>Sequence Analysis, Protein</subject><subject>Sequence Homology, Amino Acid</subject><subject>Temperature</subject><subject>Trypsin - metabolism</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkEtLAzEQgIMotlZ_gBctgt5WZ7J57VGKLygoaM8h3U3aLbubmnQP_femtFDwMsPAN6-PkGuERwSQTxGA8jwDEBmwFMQJGSLLaQYC2SkZAkqeSV6oAbmIcQWAVAlxTgYoFDBR8CHJlrb1zTbW3dgF3443Sztu-7gMPhVfje2D3_RxbMO2W9T1JTlzpon26pBHZPb68jN5z6afbx-T52lWMik3GQWZp93gOFTG4BxkJQRnqhJO8srJ0jBWAoIp5so5TtE6dNxIZQvMuZvnI_Kwn7sO_re3caPbOpa2aUxnfR81FkX6QEEC7_6BK9-HLt2mBQWmoOAiQbiHyuBjDNbpdahbE7YaQe9E6r1InUTqnUi967k5DO7nra2OHQdzCbg_ACaWpnHBdGUdj5yiebpRJe52zznjtVmExMy-KWAShKAE0vwP_BiClQ</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Ngai, Patrick H. 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K</au><au>Ng, T. B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>hemolysin from the mushroom Pleurotus eryngii</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>72</volume><issue>6</issue><spage>1185</spage><epage>1191</epage><pages>1185-1191</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>A monomeric 17-kDa hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom Pleurotus eryngii, using a protocol that involved gel filtration on Superdex 75, ion exchange chromatography on Mono Q and gel filtration on Superdex 75. Its N-terminal sequence demonstrated striking homology to that of its counterparts ostreolysin from the oyster mushroom Pleurotus ostreatus and aegerolysin from the mushroom Agrocybe cylindracea. Its hemolytic activity was unaffected over the pH range 4.0-12.0, but no activity was observed at pH 13 and at and below pH 2. The hemolysin was stable between 0 and 30 °C. At 40 °C, only residual activity was detectable. At and above 50 °C, activity was indiscernible. Eryngeolysin exhibited cytotoxicity toward leukemia (L1210) cells but not toward fungi. The hemolysin was inactivated by treatment with trypsin. It exhibited antibacterial activity against Bacillus sp. but not against other species. It inhibited basal as well as ConA-stimulated mitogenic response of murine splenocytes. N-Glycolyneuraminic acid was the only sugar capable of inhibiting the hemolytic activity. Eryngeolysin-induced hemolysis was osmotically protected by polyethylene glycol (PEG) 10000 with a mean hydrated diameter dose to 9.3 nm. However, no protection was offered by PEG 10000 to the anti-mitogenic and antiproliferative activities of eryngeolysin. The susceptibility of erythrocytes from different classes of vertebrates to eryngeolysin was mammalian > avian > reptilian > piscine.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>16804695</pmid><doi>10.1007/s00253-006-0406-6</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Applied microbiology and biotechnology, 2006-10, Vol.72 (6), p.1185-1191 |
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| language | eng |
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| subjects | Bacillus Biological and medical sciences Biotechnology Cell Proliferation - drug effects Chromatography, Gel Chromatography, Ion Exchange Cytotoxicity Electrophoresis, Polyacrylamide Gel Erythrocytes - drug effects Filtration Fruiting Bodies, Fungal - chemistry Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungi - drug effects Hemolysin Proteins - chemistry Hemolysin Proteins - isolation & purification Hemolysin Proteins - pharmacology Hemolysis Hemolytic Agents - chemistry Hemolytic Agents - isolation & purification Hemolytic Agents - pharmacology Hydrogen-Ion Concentration Leukemia Mitosis - drug effects Molecular Sequence Data Molecular Weight Mushrooms Neuraminic Acids - metabolism Pleurotus - chemistry Pleurotus eryngii Pleurotus ostreatus Polyethylene glycol Sequence Analysis, Protein Sequence Homology, Amino Acid Temperature Trypsin - metabolism |
| title | hemolysin from the mushroom Pleurotus eryngii |
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