Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns
Purpose: The aim of this study was to investigate the effects of magnetic fields (MF) of different intensity generated by a magnetic resonance (MR) unit (0.5 Tesla) and a double cylindrical coil (0.5 mTesla) on human CD4+ T cell lines. Materials and methods: CD4+ T cells were exposed for two hours u...
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| Veröffentlicht in: | International journal of radiation biology 2006-02, Vol.82 (2), p.77-85 |
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| creator | Salerno, Sergio La Mendola, Carmela Lo Casto, Antonio Mamone, Giuseppe Caccamo, Nadia Cardinale, Adelfio E. Salerno, Alfredo |
| description | Purpose: The aim of this study was to investigate the effects of magnetic fields (MF) of different intensity generated by a magnetic resonance (MR) unit (0.5 Tesla) and a double cylindrical coil (0.5 mTesla) on human CD4+ T cell lines.
Materials and methods: CD4+ T cells were exposed for two hours under isothermal conditions (37 ± 0.5°C) to the above mentioned MF; a control group was provided for each exposed sample. After exposure, the samples were analysed in the laboratory for the following endpoints: Release of cytokines, expression of surface markers, cell proliferation and levels of cytosolic free-calcium.
Results: Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen, caused a decrease of interferon-γ production, a decrease of cell proliferation, a decrease of expression of CD25 and a decrease of cytosolic free calcium concentration in exposed CD4+ T cell lines. Data obtained, were statistically significant when evaluated after 24 h of in vitro culture, but were not significant, for both types of MF, when the experimental groups were analysed after prolonged in vitro culture.
Conclusion: These results indicate that static magnetic fields (SMF) can give rise to transient biological effects on T lymphocytes and the present system is a sensitive model for understanding the effects of MF on the immune system. |
| doi_str_mv | 10.1080/09553000600589131 |
| format | Article |
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Materials and methods: CD4+ T cells were exposed for two hours under isothermal conditions (37 ± 0.5°C) to the above mentioned MF; a control group was provided for each exposed sample. After exposure, the samples were analysed in the laboratory for the following endpoints: Release of cytokines, expression of surface markers, cell proliferation and levels of cytosolic free-calcium.
Results: Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen, caused a decrease of interferon-γ production, a decrease of cell proliferation, a decrease of expression of CD25 and a decrease of cytosolic free calcium concentration in exposed CD4+ T cell lines. Data obtained, were statistically significant when evaluated after 24 h of in vitro culture, but were not significant, for both types of MF, when the experimental groups were analysed after prolonged in vitro culture.
Conclusion: These results indicate that static magnetic fields (SMF) can give rise to transient biological effects on T lymphocytes and the present system is a sensitive model for understanding the effects of MF on the immune system.</description><identifier>ISSN: 0955-3002</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553000600589131</identifier><identifier>PMID: 16546906</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Adaptation, Physiological - immunology ; Adaptation, Physiological - radiation effects ; Cell Line ; cell proliferation ; Cell Proliferation - radiation effects ; cytokines ; Cytokines - immunology ; Dose-Response Relationship, Radiation ; Electricity ; Humans ; Lymphocyte Activation - immunology ; Lymphocyte Activation - radiation effects ; lymphocytes ; Lymphocytes - immunology ; Lymphocytes - radiation effects ; Magnetic fields ; Magnetic Resonance Imaging ; Radiation Dosage</subject><ispartof>International journal of radiation biology, 2006-02, Vol.82 (2), p.77-85</ispartof><rights>2006 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-ca5ea54b60e40487c4fa3ece5d219e06ecb631ba9d61729d42a8503297384f993</citedby><cites>FETCH-LOGICAL-c435t-ca5ea54b60e40487c4fa3ece5d219e06ecb631ba9d61729d42a8503297384f993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553000600589131$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553000600589131$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16546906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salerno, Sergio</creatorcontrib><creatorcontrib>La Mendola, Carmela</creatorcontrib><creatorcontrib>Lo Casto, Antonio</creatorcontrib><creatorcontrib>Mamone, Giuseppe</creatorcontrib><creatorcontrib>Caccamo, Nadia</creatorcontrib><creatorcontrib>Cardinale, Adelfio E.</creatorcontrib><creatorcontrib>Salerno, Alfredo</creatorcontrib><title>Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol</addtitle><description>Purpose: The aim of this study was to investigate the effects of magnetic fields (MF) of different intensity generated by a magnetic resonance (MR) unit (0.5 Tesla) and a double cylindrical coil (0.5 mTesla) on human CD4+ T cell lines.
Materials and methods: CD4+ T cells were exposed for two hours under isothermal conditions (37 ± 0.5°C) to the above mentioned MF; a control group was provided for each exposed sample. After exposure, the samples were analysed in the laboratory for the following endpoints: Release of cytokines, expression of surface markers, cell proliferation and levels of cytosolic free-calcium.
Results: Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen, caused a decrease of interferon-γ production, a decrease of cell proliferation, a decrease of expression of CD25 and a decrease of cytosolic free calcium concentration in exposed CD4+ T cell lines. Data obtained, were statistically significant when evaluated after 24 h of in vitro culture, but were not significant, for both types of MF, when the experimental groups were analysed after prolonged in vitro culture.
Conclusion: These results indicate that static magnetic fields (SMF) can give rise to transient biological effects on T lymphocytes and the present system is a sensitive model for understanding the effects of MF on the immune system.</description><subject>Adaptation, Physiological - immunology</subject><subject>Adaptation, Physiological - radiation effects</subject><subject>Cell Line</subject><subject>cell proliferation</subject><subject>Cell Proliferation - radiation effects</subject><subject>cytokines</subject><subject>Cytokines - immunology</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Electricity</subject><subject>Humans</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Activation - radiation effects</subject><subject>lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Lymphocytes - radiation effects</subject><subject>Magnetic fields</subject><subject>Magnetic Resonance Imaging</subject><subject>Radiation Dosage</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFGLEzEUhYMobl39Ab5InkQfut5MJukEfZFlV4WKsNTn4U7mxk7JJDXJrPTfO7UFEWGf7oHzncPlMPZSwJWABt6BUUoCgAZQjRFSPGILIXW1lLPzmC2O_qyhumDPct7NZAWyecouhFa1NqAXbHdH95Ty0Hni5BzZwqPjX-84hp7frG_5iD8ClcFyN5DvM38DV4pv_thHNW7e8hj4dhoxcH8Y99toD4U42jLcYxlmb4-lUAr5OXvi0Gd6cb6X7Pvtzeb683L97dOX64_rpa2lKkuLilDVnQaqoW5WtnYoyZLqK2EINNlOS9Gh6bVYVaavK2wUyMqsZFM7Y-Qle33q3af4c6Jc2nHIlrzHQHHKrTBGKwGrGRQn0KaYcyLX7tMwYjq0AtrjwO1_A8-ZV-fyqRup_5s4LzoDH07AEFxMI_6KyfdtwYOPySUMdsitfKj__T_xLaEvW4uJ2l2cUpiHe-C73-rVmLc</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Salerno, Sergio</creator><creator>La Mendola, Carmela</creator><creator>Lo Casto, Antonio</creator><creator>Mamone, Giuseppe</creator><creator>Caccamo, Nadia</creator><creator>Cardinale, Adelfio E.</creator><creator>Salerno, Alfredo</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20060201</creationdate><title>Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns</title><author>Salerno, Sergio ; La Mendola, Carmela ; Lo Casto, Antonio ; Mamone, Giuseppe ; Caccamo, Nadia ; Cardinale, Adelfio E. ; Salerno, Alfredo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-ca5ea54b60e40487c4fa3ece5d219e06ecb631ba9d61729d42a8503297384f993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adaptation, Physiological - immunology</topic><topic>Adaptation, Physiological - radiation effects</topic><topic>Cell Line</topic><topic>cell proliferation</topic><topic>Cell Proliferation - radiation effects</topic><topic>cytokines</topic><topic>Cytokines - immunology</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Electricity</topic><topic>Humans</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Activation - radiation effects</topic><topic>lymphocytes</topic><topic>Lymphocytes - immunology</topic><topic>Lymphocytes - radiation effects</topic><topic>Magnetic fields</topic><topic>Magnetic Resonance Imaging</topic><topic>Radiation Dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salerno, Sergio</creatorcontrib><creatorcontrib>La Mendola, Carmela</creatorcontrib><creatorcontrib>Lo Casto, Antonio</creatorcontrib><creatorcontrib>Mamone, Giuseppe</creatorcontrib><creatorcontrib>Caccamo, Nadia</creatorcontrib><creatorcontrib>Cardinale, Adelfio E.</creatorcontrib><creatorcontrib>Salerno, Alfredo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salerno, Sergio</au><au>La Mendola, Carmela</au><au>Lo Casto, Antonio</au><au>Mamone, Giuseppe</au><au>Caccamo, Nadia</au><au>Cardinale, Adelfio E.</au><au>Salerno, Alfredo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>82</volume><issue>2</issue><spage>77</spage><epage>85</epage><pages>77-85</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>Purpose: The aim of this study was to investigate the effects of magnetic fields (MF) of different intensity generated by a magnetic resonance (MR) unit (0.5 Tesla) and a double cylindrical coil (0.5 mTesla) on human CD4+ T cell lines.
Materials and methods: CD4+ T cells were exposed for two hours under isothermal conditions (37 ± 0.5°C) to the above mentioned MF; a control group was provided for each exposed sample. After exposure, the samples were analysed in the laboratory for the following endpoints: Release of cytokines, expression of surface markers, cell proliferation and levels of cytosolic free-calcium.
Results: Exposure to MF for 2 h and subsequent in vitro stimulation in the presence of the appropriate mitogen, caused a decrease of interferon-γ production, a decrease of cell proliferation, a decrease of expression of CD25 and a decrease of cytosolic free calcium concentration in exposed CD4+ T cell lines. Data obtained, were statistically significant when evaluated after 24 h of in vitro culture, but were not significant, for both types of MF, when the experimental groups were analysed after prolonged in vitro culture.
Conclusion: These results indicate that static magnetic fields (SMF) can give rise to transient biological effects on T lymphocytes and the present system is a sensitive model for understanding the effects of MF on the immune system.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>16546906</pmid><doi>10.1080/09553000600589131</doi><tpages>9</tpages></addata></record> |
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| subjects | Adaptation, Physiological - immunology Adaptation, Physiological - radiation effects Cell Line cell proliferation Cell Proliferation - radiation effects cytokines Cytokines - immunology Dose-Response Relationship, Radiation Electricity Humans Lymphocyte Activation - immunology Lymphocyte Activation - radiation effects lymphocytes Lymphocytes - immunology Lymphocytes - radiation effects Magnetic fields Magnetic Resonance Imaging Radiation Dosage |
| title | Reversible effect of MR and ELF magnetic fields (0.5 T and 0.5 mT) on human lymphocyte activation patterns |
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