Virulence and competitiveness of Cydia pomonella granulovirus mutants: parameters that do not match
The LD50, median survival time (ST50) and virus production are virulence parameters that are commonly used to describe the biological characteristics of viruses. In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 a...
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| Veröffentlicht in: | Journal of general virology 2005-10, Vol.86 (10), p.2731-2738 |
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| creator | Arends, H.M Winstanley, D Jehle, J.A |
| description | The LD50, median survival time (ST50) and virus production are virulence parameters that are commonly used to describe the biological characteristics of viruses. In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 and -MCp5) that carry Tc1-like insect transposable elements. The three virus genotypes were similar in their LD50, ST50 and virus production. However, the mutant genotypes MCp4 and MCp5 were very effectively out-competed by CpGV-M in direct competition experiments, where Cydia pomonella larvae were co-infected with known ratios of occlusion bodies or budded virus of CpGV-M and one of the two mutants. It was demonstrated that MCp5 and MCp4 could not be sustained in the virus population when the progeny viruses of different co-infections were used as inocula to infect next passage larvae. These results show that the virulence parameters LD50, ST50 and virus production alone do not adequately reflect the competitiveness of the virus and are thus not suitable to describe virus population dynamics. |
| doi_str_mv | 10.1099/vir.0.81152-0 |
| format | Article |
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In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 and -MCp5) that carry Tc1-like insect transposable elements. The three virus genotypes were similar in their LD50, ST50 and virus production. However, the mutant genotypes MCp4 and MCp5 were very effectively out-competed by CpGV-M in direct competition experiments, where Cydia pomonella larvae were co-infected with known ratios of occlusion bodies or budded virus of CpGV-M and one of the two mutants. It was demonstrated that MCp5 and MCp4 could not be sustained in the virus population when the progeny viruses of different co-infections were used as inocula to infect next passage larvae. These results show that the virulence parameters LD50, ST50 and virus production alone do not adequately reflect the competitiveness of the virus and are thus not suitable to describe virus population dynamics.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.81152-0</identifier><identifier>PMID: 16186226</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; competitiveness ; Cydia pomonella ; Cydia pomonella granulovirus ; DNA Transposable Elements - genetics ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Granulovirus ; Granulovirus - genetics ; Granulovirus - isolation & purification ; Granulovirus - pathogenicity ; Granulovirus - physiology ; infection ; insect pests ; insect viruses ; Larva - virology ; larvae ; Lethal Dose 50 ; Malus ; Microbiology ; Miscellaneous ; Moths - growth & development ; Moths - virology ; mutants ; viral insecticides ; Virology ; virulence ; Virulence - genetics</subject><ispartof>Journal of general virology, 2005-10, Vol.86 (10), p.2731-2738</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-ee3e280fe630a150c92f88e042fee55e254cd5a8437b0eafbe960419dc33416c3</citedby><cites>FETCH-LOGICAL-c449t-ee3e280fe630a150c92f88e042fee55e254cd5a8437b0eafbe960419dc33416c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17144328$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16186226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arends, H.M</creatorcontrib><creatorcontrib>Winstanley, D</creatorcontrib><creatorcontrib>Jehle, J.A</creatorcontrib><title>Virulence and competitiveness of Cydia pomonella granulovirus mutants: parameters that do not match</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>The LD50, median survival time (ST50) and virus production are virulence parameters that are commonly used to describe the biological characteristics of viruses. In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 and -MCp5) that carry Tc1-like insect transposable elements. The three virus genotypes were similar in their LD50, ST50 and virus production. However, the mutant genotypes MCp4 and MCp5 were very effectively out-competed by CpGV-M in direct competition experiments, where Cydia pomonella larvae were co-infected with known ratios of occlusion bodies or budded virus of CpGV-M and one of the two mutants. It was demonstrated that MCp5 and MCp4 could not be sustained in the virus population when the progeny viruses of different co-infections were used as inocula to infect next passage larvae. These results show that the virulence parameters LD50, ST50 and virus production alone do not adequately reflect the competitiveness of the virus and are thus not suitable to describe virus population dynamics.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>competitiveness</subject><subject>Cydia pomonella</subject><subject>Cydia pomonella granulovirus</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulovirus</subject><subject>Granulovirus - genetics</subject><subject>Granulovirus - isolation & purification</subject><subject>Granulovirus - pathogenicity</subject><subject>Granulovirus - physiology</subject><subject>infection</subject><subject>insect pests</subject><subject>insect viruses</subject><subject>Larva - virology</subject><subject>larvae</subject><subject>Lethal Dose 50</subject><subject>Malus</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Moths - growth & development</subject><subject>Moths - virology</subject><subject>mutants</subject><subject>viral insecticides</subject><subject>Virology</subject><subject>virulence</subject><subject>Virulence - genetics</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0LtvFDEQBnALgcgRKGnBTZAo9hi_9kGHTrykSBRJaC2fd3xntLtebG-i_Pd4uZNSuZifvhl_hLxlsGXQdZ_ufdzCtmVM8QqekQ2Ttap4mTwnGwDOKyZYc0FepfQHgEmpmpfkgtWsrTmvN8T-9nEZcLJIzdRTG8YZs8_-HidMiQZHd4-9N3QOY5hwGAw9RDMtQyh7l0THJZspp890NtGMmDEmmo8m0z7QKWQ6mmyPr8kLZ4aEb87vJbn79vV296O6_vX95-7LdWWl7HKFKJC34LAWYJgC23HXtgiSO0SlkCtpe2VaKZo9oHF77GqQrOutEJLVVlySD6fcOYa_C6asR5_sevSEYUmadZ1qGwUFVidoY0gpotNz9KOJj5qBXlvV5Xca9P9W9erfnYOX_Yj9kz7XWMDVGZhkzeBKRdanJ9eU4gVvi_t4ckd_OD74iPqA0-jLGXsf1qVtvZ7AG8GKfX-yzgRtDrHk3d1wYAIYCAWNEP8A0VWZOA</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Arends, H.M</creator><creator>Winstanley, D</creator><creator>Jehle, J.A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20051001</creationdate><title>Virulence and competitiveness of Cydia pomonella granulovirus mutants: parameters that do not match</title><author>Arends, H.M ; Winstanley, D ; Jehle, J.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-ee3e280fe630a150c92f88e042fee55e254cd5a8437b0eafbe960419dc33416c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>competitiveness</topic><topic>Cydia pomonella</topic><topic>Cydia pomonella granulovirus</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulovirus</topic><topic>Granulovirus - genetics</topic><topic>Granulovirus - isolation & purification</topic><topic>Granulovirus - pathogenicity</topic><topic>Granulovirus - physiology</topic><topic>infection</topic><topic>insect pests</topic><topic>insect viruses</topic><topic>Larva - virology</topic><topic>larvae</topic><topic>Lethal Dose 50</topic><topic>Malus</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Moths - growth & development</topic><topic>Moths - virology</topic><topic>mutants</topic><topic>viral insecticides</topic><topic>Virology</topic><topic>virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arends, H.M</creatorcontrib><creatorcontrib>Winstanley, D</creatorcontrib><creatorcontrib>Jehle, J.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arends, H.M</au><au>Winstanley, D</au><au>Jehle, J.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulence and competitiveness of Cydia pomonella granulovirus mutants: parameters that do not match</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2005-10-01</date><risdate>2005</risdate><volume>86</volume><issue>10</issue><spage>2731</spage><epage>2738</epage><pages>2731-2738</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>The LD50, median survival time (ST50) and virus production are virulence parameters that are commonly used to describe the biological characteristics of viruses. In this study, these parameters were determined for Cydia pomonella granulovirus (CpGV-M) and two naturally occurring mutants (CpGV-MCp4 and -MCp5) that carry Tc1-like insect transposable elements. The three virus genotypes were similar in their LD50, ST50 and virus production. However, the mutant genotypes MCp4 and MCp5 were very effectively out-competed by CpGV-M in direct competition experiments, where Cydia pomonella larvae were co-infected with known ratios of occlusion bodies or budded virus of CpGV-M and one of the two mutants. It was demonstrated that MCp5 and MCp4 could not be sustained in the virus population when the progeny viruses of different co-infections were used as inocula to infect next passage larvae. These results show that the virulence parameters LD50, ST50 and virus production alone do not adequately reflect the competitiveness of the virus and are thus not suitable to describe virus population dynamics.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>16186226</pmid><doi>10.1099/vir.0.81152-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences competitiveness Cydia pomonella Cydia pomonella granulovirus DNA Transposable Elements - genetics DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Granulovirus Granulovirus - genetics Granulovirus - isolation & purification Granulovirus - pathogenicity Granulovirus - physiology infection insect pests insect viruses Larva - virology larvae Lethal Dose 50 Malus Microbiology Miscellaneous Moths - growth & development Moths - virology mutants viral insecticides Virology virulence Virulence - genetics |
| title | Virulence and competitiveness of Cydia pomonella granulovirus mutants: parameters that do not match |
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