A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring
Toward ultra‐simple and field‐ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a fun...
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| Veröffentlicht in: | Chemistry : a European journal 2018-04, Vol.24 (23), p.6025-6029 |
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| container_issue | 23 |
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| container_title | Chemistry : a European journal |
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| creator | Klug, Katherine E. Reynolds, Kelly A. Yoon, Jeong‐Yeol |
| description | Toward ultra‐simple and field‐ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper‐based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring.
A novel assay transducer mechanism is demonstrated based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Pathogens are quantified as a function of flow rate through a paper microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation. |
| doi_str_mv | 10.1002/chem.201800085 |
| format | Article |
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A novel assay transducer mechanism is demonstrated based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Pathogens are quantified as a function of flow rate through a paper microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation.</description><identifier>ISSN: 0947-6539</identifier><identifier>EISSN: 1521-3765</identifier><identifier>DOI: 10.1002/chem.201800085</identifier><identifier>PMID: 29405456</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>aggregation ; Assaying ; Biosensing Techniques - methods ; Biosensors ; capillary ; Capillary flow ; Chemistry ; E coli ; Environmental monitoring ; Escherichia coli - isolation & purification ; Flow velocity ; Microfluidic Analytical Techniques ; microfluidic device ; Pathogens ; Rheological properties ; RNA, Viral ; Serum - virology ; Vector-borne diseases ; Viruses ; Water analysis ; Water sampling ; Zika Virus - genetics</subject><ispartof>Chemistry : a European journal, 2018-04, Vol.24 (23), p.6025-6029</ispartof><rights>2018 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4505-dedb2c5a07191c6d08f468df9150037daccd7c157f61a61da75c2243806a072f3</citedby><cites>FETCH-LOGICAL-c4505-dedb2c5a07191c6d08f468df9150037daccd7c157f61a61da75c2243806a072f3</cites><orcidid>0000-0002-1728-4623 ; 0000-0002-9720-6472</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fchem.201800085$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fchem.201800085$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29405456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klug, Katherine E.</creatorcontrib><creatorcontrib>Reynolds, Kelly A.</creatorcontrib><creatorcontrib>Yoon, Jeong‐Yeol</creatorcontrib><title>A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring</title><title>Chemistry : a European journal</title><addtitle>Chemistry</addtitle><description>Toward ultra‐simple and field‐ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Differential capillary flow is tunable, allowing pathogen quantification as a function of flow rate through a paper‐based microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation. This mechanism is demonstrated for assays of Escherichia coli K12 in water samples and Zika virus (ZIKV) in blood serum. These assays achieved very low limits of detection compared with other demonstrated methods (1 log CFU/mL E. coli and 20 pg/mL ZIKV whole virus) with an operating time of 30 s, showing promise for environmental and health monitoring.
A novel assay transducer mechanism is demonstrated based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Pathogens are quantified as a function of flow rate through a paper microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation.</description><subject>aggregation</subject><subject>Assaying</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>capillary</subject><subject>Capillary flow</subject><subject>Chemistry</subject><subject>E coli</subject><subject>Environmental monitoring</subject><subject>Escherichia coli - isolation & purification</subject><subject>Flow velocity</subject><subject>Microfluidic Analytical Techniques</subject><subject>microfluidic device</subject><subject>Pathogens</subject><subject>Rheological properties</subject><subject>RNA, Viral</subject><subject>Serum - virology</subject><subject>Vector-borne diseases</subject><subject>Viruses</subject><subject>Water analysis</subject><subject>Water sampling</subject><subject>Zika Virus - genetics</subject><issn>0947-6539</issn><issn>1521-3765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkL1OHDEURq2IKCyQNiWyRJNmNteesT0uybL8SKBEAmrL2B4wmrE39mzQdnmEPCNPglfLj0RD5eZ8R9cHoW8EpgSA_jB3bphSIC0AtOwTmhBGSVULzrbQBGQjKs5quY12cr4viOR1_QVtU9kAaxifIHWIZ3rh-16nFT7u4wM-WgU9eJMf__3_qbOz-NKF7MMtvohW935c4S4mfOSTMyOeh78-xTC4MOoe_9bjXbx1oaDBjzGV1R763Ok-u6_P7y66Pp5fzU6r818nZ7PD88o0DFhlnb2hhmkQRBLDLbRdw1vbScIAamG1MVYYwkTHiebEasEMpU3dAi8b2tW76PvGu0jxz9LlUQ0-G1f-FVxcZkWkZIQRoKSgB-_Q-7hMoVynKFDBWtE2a2q6oUyKOSfXqUXyQ6mkCKh1erVOr17Tl8H-s3Z5Mzj7ir-0LoDcAA--d6sPdGp2Or94kz8BcDKQUw</recordid><startdate>20180420</startdate><enddate>20180420</enddate><creator>Klug, Katherine E.</creator><creator>Reynolds, Kelly A.</creator><creator>Yoon, Jeong‐Yeol</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1728-4623</orcidid><orcidid>https://orcid.org/0000-0002-9720-6472</orcidid></search><sort><creationdate>20180420</creationdate><title>A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring</title><author>Klug, Katherine E. ; Reynolds, Kelly A. ; Yoon, Jeong‐Yeol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4505-dedb2c5a07191c6d08f468df9150037daccd7c157f61a61da75c2243806a072f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>aggregation</topic><topic>Assaying</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>capillary</topic><topic>Capillary flow</topic><topic>Chemistry</topic><topic>E coli</topic><topic>Environmental monitoring</topic><topic>Escherichia coli - isolation & purification</topic><topic>Flow velocity</topic><topic>Microfluidic Analytical Techniques</topic><topic>microfluidic device</topic><topic>Pathogens</topic><topic>Rheological properties</topic><topic>RNA, Viral</topic><topic>Serum - virology</topic><topic>Vector-borne diseases</topic><topic>Viruses</topic><topic>Water analysis</topic><topic>Water sampling</topic><topic>Zika Virus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klug, Katherine E.</creatorcontrib><creatorcontrib>Reynolds, Kelly A.</creatorcontrib><creatorcontrib>Yoon, Jeong‐Yeol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry : a European journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klug, Katherine E.</au><au>Reynolds, Kelly A.</au><au>Yoon, Jeong‐Yeol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring</atitle><jtitle>Chemistry : a European journal</jtitle><addtitle>Chemistry</addtitle><date>2018-04-20</date><risdate>2018</risdate><volume>24</volume><issue>23</issue><spage>6025</spage><epage>6029</epage><pages>6025-6029</pages><issn>0947-6539</issn><eissn>1521-3765</eissn><abstract>Toward ultra‐simple and field‐ready biosensors, we demonstrate a novel assay transducer mechanism based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. 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A novel assay transducer mechanism is demonstrated based on interfacial property changes and capillary flow dynamics in antibody‐conjugated submicron particle suspensions. Pathogens are quantified as a function of flow rate through a paper microfluidic device. Flow models based on interfacial and rheological properties indicate a significant relationship between the flow rate and the interfacial effects caused by target‐particle aggregation.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29405456</pmid><doi>10.1002/chem.201800085</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1728-4623</orcidid><orcidid>https://orcid.org/0000-0002-9720-6472</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library |
| subjects | aggregation Assaying Biosensing Techniques - methods Biosensors capillary Capillary flow Chemistry E coli Environmental monitoring Escherichia coli - isolation & purification Flow velocity Microfluidic Analytical Techniques microfluidic device Pathogens Rheological properties RNA, Viral Serum - virology Vector-borne diseases Viruses Water analysis Water sampling Zika Virus - genetics |
| title | A Capillary Flow Dynamics‐Based Sensing Modality for Direct Environmental Pathogen Monitoring |
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