Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair
Objective The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and Methods Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to an...
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| Veröffentlicht in: | Lasers in surgery and medicine 2018-09, Vol.50 (7), p.781-791 |
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| creator | Andreo, Lucas Mesquita‐Ferrari, Raquel A. Ribeiro, Beatriz G. Benitte, Adriana de Fátima Nogueira, Tatiane França, Cristiane M. Silva, Daniela de Fátima Teixeira da Bussadori, Sandra K. Fernandes, Kristianne P.S. Corrêa, Fernanda I. Corrêa, João C.F. |
| description | Objective
The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury.
Materials and Methods
Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages.
Results
No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days.
Conclusion
Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018. © 2018 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/lsm.22798 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1994363072</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1994363072</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3908-acdd2d4590e5302aa0d54f5b003aaca0fd7bd4a22247eb897627d2f05fdcb0e63</originalsourceid><addsrcrecordid>eNp10cFu1DAQBmALgei2cOAFkCUu7WHb8SRZx0e0KgVpEQfgbDn2GLJy4mAnrfbGI_CMPAkuWzggcfIcPv2a8c_YCwGXAgCvQh4uEaVqH7GVALVZKwHiMVuBKHMLCk_Yac57AKgQ5FN2gqpSqq3liu2vvSc7Zx49Hw7xC4295VMiu6QcE7cUQubnW9wKvOBzMmOeghlnM_dx5GZ0PMS7n99_BLqlwIPJlPj8lZKZDryAYck2EE80mT49Y0-8CZmeP7xn7POb60_bt-vdh5t329e7ta0UtGtjnUNXNwqoqQCNAdfUvunK8sZYA97JztUGEWtJXavkBqVDD413tgPaVGfs_Jg7pfhtoTzroc_3h5iR4pK1UKquNhVILPTVP3QflzSW7TSCApAtiqaoi6OyKeacyOsp9YNJBy1A3xegSwH6dwHFvnxIXLqB3F_558cLuDqCuz7Q4f9Jevfx_THyF3jgkMc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2090078215</pqid></control><display><type>article</type><title>Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair</title><source>Wiley Online Library All Journals</source><creator>Andreo, Lucas ; Mesquita‐Ferrari, Raquel A. ; Ribeiro, Beatriz G. ; Benitte, Adriana ; de Fátima Nogueira, Tatiane ; França, Cristiane M. ; Silva, Daniela de Fátima Teixeira da ; Bussadori, Sandra K. ; Fernandes, Kristianne P.S. ; Corrêa, Fernanda I. ; Corrêa, João C.F.</creator><creatorcontrib>Andreo, Lucas ; Mesquita‐Ferrari, Raquel A. ; Ribeiro, Beatriz G. ; Benitte, Adriana ; de Fátima Nogueira, Tatiane ; França, Cristiane M. ; Silva, Daniela de Fátima Teixeira da ; Bussadori, Sandra K. ; Fernandes, Kristianne P.S. ; Corrêa, Fernanda I. ; Corrêa, João C.F.</creatorcontrib><description>Objective
The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury.
Materials and Methods
Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages.
Results
No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days.
Conclusion
Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018. © 2018 Wiley Periodicals, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.22798</identifier><identifier>PMID: 29399847</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Blood vessels ; cell transplantation ; Collagen ; Cryoinjury ; Inflammation ; Injuries ; Inoculation ; laser therapy ; Lasers ; Macrophages ; Muscles ; Musculoskeletal system ; myoblasts ; Myonecrosis ; Precursors ; Repair ; Rodents ; Saline solutions ; Skeletal muscle ; Therapy ; Tibialis anterior muscle ; Transplantation</subject><ispartof>Lasers in surgery and medicine, 2018-09, Vol.50 (7), p.781-791</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3908-acdd2d4590e5302aa0d54f5b003aaca0fd7bd4a22247eb897627d2f05fdcb0e63</citedby><cites>FETCH-LOGICAL-c3908-acdd2d4590e5302aa0d54f5b003aaca0fd7bd4a22247eb897627d2f05fdcb0e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.22798$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.22798$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29399847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andreo, Lucas</creatorcontrib><creatorcontrib>Mesquita‐Ferrari, Raquel A.</creatorcontrib><creatorcontrib>Ribeiro, Beatriz G.</creatorcontrib><creatorcontrib>Benitte, Adriana</creatorcontrib><creatorcontrib>de Fátima Nogueira, Tatiane</creatorcontrib><creatorcontrib>França, Cristiane M.</creatorcontrib><creatorcontrib>Silva, Daniela de Fátima Teixeira da</creatorcontrib><creatorcontrib>Bussadori, Sandra K.</creatorcontrib><creatorcontrib>Fernandes, Kristianne P.S.</creatorcontrib><creatorcontrib>Corrêa, Fernanda I.</creatorcontrib><creatorcontrib>Corrêa, João C.F.</creatorcontrib><title>Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg Med</addtitle><description>Objective
The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury.
Materials and Methods
Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages.
Results
No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days.
Conclusion
Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018. © 2018 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Blood vessels</subject><subject>cell transplantation</subject><subject>Collagen</subject><subject>Cryoinjury</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Inoculation</subject><subject>laser therapy</subject><subject>Lasers</subject><subject>Macrophages</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>myoblasts</subject><subject>Myonecrosis</subject><subject>Precursors</subject><subject>Repair</subject><subject>Rodents</subject><subject>Saline solutions</subject><subject>Skeletal muscle</subject><subject>Therapy</subject><subject>Tibialis anterior muscle</subject><subject>Transplantation</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp10cFu1DAQBmALgei2cOAFkCUu7WHb8SRZx0e0KgVpEQfgbDn2GLJy4mAnrfbGI_CMPAkuWzggcfIcPv2a8c_YCwGXAgCvQh4uEaVqH7GVALVZKwHiMVuBKHMLCk_Yac57AKgQ5FN2gqpSqq3liu2vvSc7Zx49Hw7xC4295VMiu6QcE7cUQubnW9wKvOBzMmOeghlnM_dx5GZ0PMS7n99_BLqlwIPJlPj8lZKZDryAYck2EE80mT49Y0-8CZmeP7xn7POb60_bt-vdh5t329e7ta0UtGtjnUNXNwqoqQCNAdfUvunK8sZYA97JztUGEWtJXavkBqVDD413tgPaVGfs_Jg7pfhtoTzroc_3h5iR4pK1UKquNhVILPTVP3QflzSW7TSCApAtiqaoi6OyKeacyOsp9YNJBy1A3xegSwH6dwHFvnxIXLqB3F_558cLuDqCuz7Q4f9Jevfx_THyF3jgkMc</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Andreo, Lucas</creator><creator>Mesquita‐Ferrari, Raquel A.</creator><creator>Ribeiro, Beatriz G.</creator><creator>Benitte, Adriana</creator><creator>de Fátima Nogueira, Tatiane</creator><creator>França, Cristiane M.</creator><creator>Silva, Daniela de Fátima Teixeira da</creator><creator>Bussadori, Sandra K.</creator><creator>Fernandes, Kristianne P.S.</creator><creator>Corrêa, Fernanda I.</creator><creator>Corrêa, João C.F.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201809</creationdate><title>Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair</title><author>Andreo, Lucas ; Mesquita‐Ferrari, Raquel A. ; Ribeiro, Beatriz G. ; Benitte, Adriana ; de Fátima Nogueira, Tatiane ; França, Cristiane M. ; Silva, Daniela de Fátima Teixeira da ; Bussadori, Sandra K. ; Fernandes, Kristianne P.S. ; Corrêa, Fernanda I. ; Corrêa, João C.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3908-acdd2d4590e5302aa0d54f5b003aaca0fd7bd4a22247eb897627d2f05fdcb0e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Blood vessels</topic><topic>cell transplantation</topic><topic>Collagen</topic><topic>Cryoinjury</topic><topic>Inflammation</topic><topic>Injuries</topic><topic>Inoculation</topic><topic>laser therapy</topic><topic>Lasers</topic><topic>Macrophages</topic><topic>Muscles</topic><topic>Musculoskeletal system</topic><topic>myoblasts</topic><topic>Myonecrosis</topic><topic>Precursors</topic><topic>Repair</topic><topic>Rodents</topic><topic>Saline solutions</topic><topic>Skeletal muscle</topic><topic>Therapy</topic><topic>Tibialis anterior muscle</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andreo, Lucas</creatorcontrib><creatorcontrib>Mesquita‐Ferrari, Raquel A.</creatorcontrib><creatorcontrib>Ribeiro, Beatriz G.</creatorcontrib><creatorcontrib>Benitte, Adriana</creatorcontrib><creatorcontrib>de Fátima Nogueira, Tatiane</creatorcontrib><creatorcontrib>França, Cristiane M.</creatorcontrib><creatorcontrib>Silva, Daniela de Fátima Teixeira da</creatorcontrib><creatorcontrib>Bussadori, Sandra K.</creatorcontrib><creatorcontrib>Fernandes, Kristianne P.S.</creatorcontrib><creatorcontrib>Corrêa, Fernanda I.</creatorcontrib><creatorcontrib>Corrêa, João C.F.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andreo, Lucas</au><au>Mesquita‐Ferrari, Raquel A.</au><au>Ribeiro, Beatriz G.</au><au>Benitte, Adriana</au><au>de Fátima Nogueira, Tatiane</au><au>França, Cristiane M.</au><au>Silva, Daniela de Fátima Teixeira da</au><au>Bussadori, Sandra K.</au><au>Fernandes, Kristianne P.S.</au><au>Corrêa, Fernanda I.</au><au>Corrêa, João C.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg Med</addtitle><date>2018-09</date><risdate>2018</risdate><volume>50</volume><issue>7</issue><spage>781</spage><epage>791</epage><pages>781-791</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><abstract>Objective
The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury.
Materials and Methods
Sixty‐five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages.
Results
No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days.
Conclusion
Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018. © 2018 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29399847</pmid><doi>10.1002/lsm.22798</doi><tpages>11</tpages></addata></record> |
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| ispartof | Lasers in surgery and medicine, 2018-09, Vol.50 (7), p.781-791 |
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| language | eng |
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| source | Wiley Online Library All Journals |
| subjects | Animals Blood vessels cell transplantation Collagen Cryoinjury Inflammation Injuries Inoculation laser therapy Lasers Macrophages Muscles Musculoskeletal system myoblasts Myonecrosis Precursors Repair Rodents Saline solutions Skeletal muscle Therapy Tibialis anterior muscle Transplantation |
| title | Effects of myogenic precursor cells (C2C12) transplantation and low‐level laser therapy on muscle repair |
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