Bacterial communities and enzyme activities of PAHs polluted soils
Three soils (i.e. a Belgian soil, B-BT, a German soil, G, and an Italian agricultural soil, I-BT) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. A chemical and microbiological evaluation of soils was done using me...
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| Veröffentlicht in: | Chemosphere (Oxford) 2004-11, Vol.57 (5), p.401-412 |
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| creator | Andreoni, V. Cavalca, L. Rao, M.A. Nocerino, G. Bernasconi, S. Dell’Amico, E. Colombo, M. Gianfreda, L. |
| description | Three soils (i.e. a Belgian soil, B-BT, a German soil, G, and an Italian agricultural soil, I-BT) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. A chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. The three soils showed different levels of polycyclic aromatic hydrocarbons (PAHs), being their contamination strictly associated to their pollution history. High values of enzyme activities and culturable heterotrophic bacteria were detected in the soil with no or negligible presence of organic pollutants. Genetic diversity of soil samples and enrichment cultures was measured as bands on denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences from the soil and enrichment community DNAs. When analysed by Shannon index (
H′), the highest genetic biodiversity (
H′
=
2.87) was found in the Belgian soil B-BT with a medium-term exposition to PAHs and the poorest biodiversity (
H′
=
0.85) in the German soil with a long-term exposition to alkanes and PAHs and where absence, or lower levels of enzyme activities were measured. For the Italian agricultural soil I-BT, containing negligible amounts of organic pollutants but the highest Cu content, a Shannon index
=
2.13 was found.
The enrichment of four mixed cultures capable of degrading solid phenanthrene in batch liquid systems was also studied. Phenanthrene degradation rates in batch systems were culture-dependent, and simple (one-slope) and complex (two-slope) kinetic behaviours were observed. The presence of common bands of microbial species in the cultures and in the native soil DNA indicated that those strains could be potential in situ phenanthrene degraders. Consistent with this assumption are the decrease of PAH and phenanthrene contents of Belgian soil B-BT and the isolation of phenanthrene-degrading bacteria.
From the fastest phenanthrene-degrading culture
C
B-BT, representative strains were identified as
Achromobacter xylosoxidans (100%),
Methylobacterium sp. (99%),
Rhizobium galegae (99%),
Rhodococcus aetherovorans (100%),
Stenotrophomonas acidaminiphila (100%),
Alcaligenes sp. (99%) and
Aquamicrobium defluvium (100%). DGGE-profiles of culture C
B-BT showed bands attributable to
Rhodococcus,
Achromobacter,
Methylobacterium rhizobium,
Alcaligenes and
Aquamicrobium.
The isolation of
Rhodococcus aeth |
| doi_str_mv | 10.1016/j.chemosphere.2004.06.013 |
| format | Article |
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H′), the highest genetic biodiversity (
H′
=
2.87) was found in the Belgian soil B-BT with a medium-term exposition to PAHs and the poorest biodiversity (
H′
=
0.85) in the German soil with a long-term exposition to alkanes and PAHs and where absence, or lower levels of enzyme activities were measured. For the Italian agricultural soil I-BT, containing negligible amounts of organic pollutants but the highest Cu content, a Shannon index
=
2.13 was found.
The enrichment of four mixed cultures capable of degrading solid phenanthrene in batch liquid systems was also studied. Phenanthrene degradation rates in batch systems were culture-dependent, and simple (one-slope) and complex (two-slope) kinetic behaviours were observed. The presence of common bands of microbial species in the cultures and in the native soil DNA indicated that those strains could be potential in situ phenanthrene degraders. Consistent with this assumption are the decrease of PAH and phenanthrene contents of Belgian soil B-BT and the isolation of phenanthrene-degrading bacteria.
From the fastest phenanthrene-degrading culture
C
B-BT, representative strains were identified as
Achromobacter xylosoxidans (100%),
Methylobacterium sp. (99%),
Rhizobium galegae (99%),
Rhodococcus aetherovorans (100%),
Stenotrophomonas acidaminiphila (100%),
Alcaligenes sp. (99%) and
Aquamicrobium defluvium (100%). DGGE-profiles of culture C
B-BT showed bands attributable to
Rhodococcus,
Achromobacter,
Methylobacterium rhizobium,
Alcaligenes and
Aquamicrobium.
The isolation of
Rhodococcus aetherovorans and
Methylobacterium sp. can be consistent with the hypothesis that different phenanthrene-degrading strategies, cell surface properties, or the presence of xenobiotic-specific membrane carriers could play a role in the uptake/degradation of solid phenanthrene.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2004.06.013</identifier><identifier>PMID: 15331267</identifier><identifier>CODEN: CMSHAF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Achromobacter ; Achromobacter xylosoxidans ; agricultural soils ; Alcaligenes ; Applied sciences ; Aquamicrobium defluvium ; Bacteria ; Bacteria - enzymology ; Bacteria - genetics ; Bacterial diversity ; Batch liquid systems ; biodegradation ; Biodegradation of pollutants ; Biodiversity ; biological activity in soil ; Biological and medical sciences ; Biotechnology ; Chromatography, Gas ; Colony Count, Microbial ; community ecology ; Decontamination. Miscellaneous ; DGGE ; DNA, Ribosomal - genetics ; Earth sciences ; Earth, ocean, space ; Electrophoresis, Polyacrylamide Gel ; Engineering and environment geology. Geothermics ; Environment and pollution ; Europe ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Geography ; Industrial applications and implications. Economical aspects ; Methylobacterium ; phenanthrene ; Phenanthrene consumption ; Phenanthrenes - metabolism ; polluted soils ; Pollution ; Pollution, environment geology ; polycyclic aromatic hydrocarbons ; Polycyclic Aromatic Hydrocarbons - metabolism ; Rhizobium ; Rhizobium galegae ; Rhodococcus ; Sequence Analysis, DNA ; Soil and sediments pollution ; soil bacteria ; Soil chemical/enzymatic characteristics ; soil enzymes ; Soil Microbiology ; Soil Pollutants - analysis ; soil pollution ; species diversity ; Stenotrophomonas</subject><ispartof>Chemosphere (Oxford), 2004-11, Vol.57 (5), p.401-412</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a491t-376d576366cca8bb12008a356542af8e988d81c6857754c167493bd8d2b6e5ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S004565350400462X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16071435$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15331267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andreoni, V.</creatorcontrib><creatorcontrib>Cavalca, L.</creatorcontrib><creatorcontrib>Rao, M.A.</creatorcontrib><creatorcontrib>Nocerino, G.</creatorcontrib><creatorcontrib>Bernasconi, S.</creatorcontrib><creatorcontrib>Dell’Amico, E.</creatorcontrib><creatorcontrib>Colombo, M.</creatorcontrib><creatorcontrib>Gianfreda, L.</creatorcontrib><title>Bacterial communities and enzyme activities of PAHs polluted soils</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>Three soils (i.e. a Belgian soil, B-BT, a German soil, G, and an Italian agricultural soil, I-BT) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. A chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. The three soils showed different levels of polycyclic aromatic hydrocarbons (PAHs), being their contamination strictly associated to their pollution history. High values of enzyme activities and culturable heterotrophic bacteria were detected in the soil with no or negligible presence of organic pollutants. Genetic diversity of soil samples and enrichment cultures was measured as bands on denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences from the soil and enrichment community DNAs. When analysed by Shannon index (
H′), the highest genetic biodiversity (
H′
=
2.87) was found in the Belgian soil B-BT with a medium-term exposition to PAHs and the poorest biodiversity (
H′
=
0.85) in the German soil with a long-term exposition to alkanes and PAHs and where absence, or lower levels of enzyme activities were measured. For the Italian agricultural soil I-BT, containing negligible amounts of organic pollutants but the highest Cu content, a Shannon index
=
2.13 was found.
The enrichment of four mixed cultures capable of degrading solid phenanthrene in batch liquid systems was also studied. Phenanthrene degradation rates in batch systems were culture-dependent, and simple (one-slope) and complex (two-slope) kinetic behaviours were observed. The presence of common bands of microbial species in the cultures and in the native soil DNA indicated that those strains could be potential in situ phenanthrene degraders. Consistent with this assumption are the decrease of PAH and phenanthrene contents of Belgian soil B-BT and the isolation of phenanthrene-degrading bacteria.
From the fastest phenanthrene-degrading culture
C
B-BT, representative strains were identified as
Achromobacter xylosoxidans (100%),
Methylobacterium sp. (99%),
Rhizobium galegae (99%),
Rhodococcus aetherovorans (100%),
Stenotrophomonas acidaminiphila (100%),
Alcaligenes sp. (99%) and
Aquamicrobium defluvium (100%). DGGE-profiles of culture C
B-BT showed bands attributable to
Rhodococcus,
Achromobacter,
Methylobacterium rhizobium,
Alcaligenes and
Aquamicrobium.
The isolation of
Rhodococcus aetherovorans and
Methylobacterium sp. can be consistent with the hypothesis that different phenanthrene-degrading strategies, cell surface properties, or the presence of xenobiotic-specific membrane carriers could play a role in the uptake/degradation of solid phenanthrene.</description><subject>Achromobacter</subject><subject>Achromobacter xylosoxidans</subject><subject>agricultural soils</subject><subject>Alcaligenes</subject><subject>Applied sciences</subject><subject>Aquamicrobium defluvium</subject><subject>Bacteria</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Bacterial diversity</subject><subject>Batch liquid systems</subject><subject>biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biodiversity</subject><subject>biological activity in soil</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromatography, Gas</subject><subject>Colony Count, Microbial</subject><subject>community ecology</subject><subject>Decontamination. Miscellaneous</subject><subject>DGGE</subject><subject>DNA, Ribosomal - genetics</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Engineering and environment geology. Geothermics</subject><subject>Environment and pollution</subject><subject>Europe</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Geography</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Methylobacterium</subject><subject>phenanthrene</subject><subject>Phenanthrene consumption</subject><subject>Phenanthrenes - metabolism</subject><subject>polluted soils</subject><subject>Pollution</subject><subject>Pollution, environment geology</subject><subject>polycyclic aromatic hydrocarbons</subject><subject>Polycyclic Aromatic Hydrocarbons - metabolism</subject><subject>Rhizobium</subject><subject>Rhizobium galegae</subject><subject>Rhodococcus</subject><subject>Sequence Analysis, DNA</subject><subject>Soil and sediments pollution</subject><subject>soil bacteria</subject><subject>Soil chemical/enzymatic characteristics</subject><subject>soil enzymes</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - analysis</subject><subject>soil pollution</subject><subject>species diversity</subject><subject>Stenotrophomonas</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1O3DAURq2qqExpX6GkC9gl2HH8t4QRFKSRQGpnbTn2TfEoiQc7QaJPj2lGosuuLN17Pn9XB6HvBFcEE36xq-wjDCHtHyFCVWPcVJhXmNAPaEWkUCWplfyIVnnBSs4oO0afU9phnMNMfULHhFFKai5W6OrK2AmiN31hwzDMo588pMKMroDxz8sARd7752UauuLh8jYV-9D38wSuSMH36Qs66kyf4OvhPUHbm-tf69tyc__jbn25KU2jyFRSwR0TnHJurZFtS_Ld0lDGWVObToKS0kliuWRCsMYSLhpFWydd3XJgFugJOl_-3cfwNEOa9OCThb43I4Q5aaIU5U0tMqgW0MaQUoRO76MfTHzRBOs3gXqn_xGo3wRqzHUWmLPfDiVzO4B7Tx6MZeDsAJhkTd9FM1qf3jmOBWkoy9zpwnUmaPM7Zmb7s84VGCtJyd-q9UJAlvbsIepkPYwWnI9gJ-2C_4-DXwEv850E</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Andreoni, V.</creator><creator>Cavalca, L.</creator><creator>Rao, M.A.</creator><creator>Nocerino, G.</creator><creator>Bernasconi, S.</creator><creator>Dell’Amico, E.</creator><creator>Colombo, M.</creator><creator>Gianfreda, L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TV</scope><scope>7U6</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20041101</creationdate><title>Bacterial communities and enzyme activities of PAHs polluted soils</title><author>Andreoni, V. ; Cavalca, L. ; Rao, M.A. ; Nocerino, G. ; Bernasconi, S. ; Dell’Amico, E. ; Colombo, M. ; Gianfreda, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a491t-376d576366cca8bb12008a356542af8e988d81c6857754c167493bd8d2b6e5ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Achromobacter</topic><topic>Achromobacter xylosoxidans</topic><topic>agricultural soils</topic><topic>Alcaligenes</topic><topic>Applied sciences</topic><topic>Aquamicrobium defluvium</topic><topic>Bacteria</topic><topic>Bacteria - enzymology</topic><topic>Bacteria - genetics</topic><topic>Bacterial diversity</topic><topic>Batch liquid systems</topic><topic>biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biodiversity</topic><topic>biological activity in soil</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chromatography, Gas</topic><topic>Colony Count, Microbial</topic><topic>community ecology</topic><topic>Decontamination. Miscellaneous</topic><topic>DGGE</topic><topic>DNA, Ribosomal - genetics</topic><topic>Earth sciences</topic><topic>Earth, ocean, space</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Engineering and environment geology. Geothermics</topic><topic>Environment and pollution</topic><topic>Europe</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Geography</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Methylobacterium</topic><topic>phenanthrene</topic><topic>Phenanthrene consumption</topic><topic>Phenanthrenes - metabolism</topic><topic>polluted soils</topic><topic>Pollution</topic><topic>Pollution, environment geology</topic><topic>polycyclic aromatic hydrocarbons</topic><topic>Polycyclic Aromatic Hydrocarbons - metabolism</topic><topic>Rhizobium</topic><topic>Rhizobium galegae</topic><topic>Rhodococcus</topic><topic>Sequence Analysis, DNA</topic><topic>Soil and sediments pollution</topic><topic>soil bacteria</topic><topic>Soil chemical/enzymatic characteristics</topic><topic>soil enzymes</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - analysis</topic><topic>soil pollution</topic><topic>species diversity</topic><topic>Stenotrophomonas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andreoni, V.</creatorcontrib><creatorcontrib>Cavalca, L.</creatorcontrib><creatorcontrib>Rao, M.A.</creatorcontrib><creatorcontrib>Nocerino, G.</creatorcontrib><creatorcontrib>Bernasconi, S.</creatorcontrib><creatorcontrib>Dell’Amico, E.</creatorcontrib><creatorcontrib>Colombo, M.</creatorcontrib><creatorcontrib>Gianfreda, L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andreoni, V.</au><au>Cavalca, L.</au><au>Rao, M.A.</au><au>Nocerino, G.</au><au>Bernasconi, S.</au><au>Dell’Amico, E.</au><au>Colombo, M.</au><au>Gianfreda, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial communities and enzyme activities of PAHs polluted soils</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>57</volume><issue>5</issue><spage>401</spage><epage>412</epage><pages>401-412</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><coden>CMSHAF</coden><abstract>Three soils (i.e. a Belgian soil, B-BT, a German soil, G, and an Italian agricultural soil, I-BT) with different properties and hydrocarbon-pollution history with regard to their potential to degrade phenanthrene were investigated. A chemical and microbiological evaluation of soils was done using measurements of routine chemical properties, bacterial counts and several enzyme activities. The three soils showed different levels of polycyclic aromatic hydrocarbons (PAHs), being their contamination strictly associated to their pollution history. High values of enzyme activities and culturable heterotrophic bacteria were detected in the soil with no or negligible presence of organic pollutants. Genetic diversity of soil samples and enrichment cultures was measured as bands on denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA sequences from the soil and enrichment community DNAs. When analysed by Shannon index (
H′), the highest genetic biodiversity (
H′
=
2.87) was found in the Belgian soil B-BT with a medium-term exposition to PAHs and the poorest biodiversity (
H′
=
0.85) in the German soil with a long-term exposition to alkanes and PAHs and where absence, or lower levels of enzyme activities were measured. For the Italian agricultural soil I-BT, containing negligible amounts of organic pollutants but the highest Cu content, a Shannon index
=
2.13 was found.
The enrichment of four mixed cultures capable of degrading solid phenanthrene in batch liquid systems was also studied. Phenanthrene degradation rates in batch systems were culture-dependent, and simple (one-slope) and complex (two-slope) kinetic behaviours were observed. The presence of common bands of microbial species in the cultures and in the native soil DNA indicated that those strains could be potential in situ phenanthrene degraders. Consistent with this assumption are the decrease of PAH and phenanthrene contents of Belgian soil B-BT and the isolation of phenanthrene-degrading bacteria.
From the fastest phenanthrene-degrading culture
C
B-BT, representative strains were identified as
Achromobacter xylosoxidans (100%),
Methylobacterium sp. (99%),
Rhizobium galegae (99%),
Rhodococcus aetherovorans (100%),
Stenotrophomonas acidaminiphila (100%),
Alcaligenes sp. (99%) and
Aquamicrobium defluvium (100%). DGGE-profiles of culture C
B-BT showed bands attributable to
Rhodococcus,
Achromobacter,
Methylobacterium rhizobium,
Alcaligenes and
Aquamicrobium.
The isolation of
Rhodococcus aetherovorans and
Methylobacterium sp. can be consistent with the hypothesis that different phenanthrene-degrading strategies, cell surface properties, or the presence of xenobiotic-specific membrane carriers could play a role in the uptake/degradation of solid phenanthrene.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15331267</pmid><doi>10.1016/j.chemosphere.2004.06.013</doi><tpages>12</tpages></addata></record> |
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| ispartof | Chemosphere (Oxford), 2004-11, Vol.57 (5), p.401-412 |
| issn | 0045-6535 1879-1298 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Achromobacter Achromobacter xylosoxidans agricultural soils Alcaligenes Applied sciences Aquamicrobium defluvium Bacteria Bacteria - enzymology Bacteria - genetics Bacterial diversity Batch liquid systems biodegradation Biodegradation of pollutants Biodiversity biological activity in soil Biological and medical sciences Biotechnology Chromatography, Gas Colony Count, Microbial community ecology Decontamination. Miscellaneous DGGE DNA, Ribosomal - genetics Earth sciences Earth, ocean, space Electrophoresis, Polyacrylamide Gel Engineering and environment geology. Geothermics Environment and pollution Europe Exact sciences and technology Fundamental and applied biological sciences. Psychology Geography Industrial applications and implications. Economical aspects Methylobacterium phenanthrene Phenanthrene consumption Phenanthrenes - metabolism polluted soils Pollution Pollution, environment geology polycyclic aromatic hydrocarbons Polycyclic Aromatic Hydrocarbons - metabolism Rhizobium Rhizobium galegae Rhodococcus Sequence Analysis, DNA Soil and sediments pollution soil bacteria Soil chemical/enzymatic characteristics soil enzymes Soil Microbiology Soil Pollutants - analysis soil pollution species diversity Stenotrophomonas |
| title | Bacterial communities and enzyme activities of PAHs polluted soils |
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