Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States
Aims To investigate the molecular epidemiology, especially temporal variations in genotype distribution, of sapoviruses and Aichi virus 1 (AiV‐1) in Arizona, United States, by examining wastewater. Methods and Results A total of 26 wastewater samples (13 influent and 13 effluent) were collected mont...
Gespeichert in:
| Veröffentlicht in: | Journal of applied microbiology 2018-05, Vol.124 (5), p.1324-1332 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1332 |
|---|---|
| container_issue | 5 |
| container_start_page | 1324 |
| container_title | Journal of applied microbiology |
| container_volume | 124 |
| creator | Kitajima, M. Rachmadi, A.T. Iker, B.C. Haramoto, E. Gerba, C.P. |
| description | Aims
To investigate the molecular epidemiology, especially temporal variations in genotype distribution, of sapoviruses and Aichi virus 1 (AiV‐1) in Arizona, United States, by examining wastewater.
Methods and Results
A total of 26 wastewater samples (13 influent and 13 effluent) were collected monthly from a wastewater treatment plant and viral strains were identified through nested reverse transcription‐PCR followed by cloning and sequencing analysis. Identified sapovirus strains were classified into seven genotypes belonging to three genogroups (GI, GII, and GV): GI.1, GI.2, GI.3, GII.1, GII.2, GII.8 and GV.1, with a clear temporal shift. The majority of AiV‐1 strains identified from the wastewater samples were classified into genotype B, and genotype A strains were identified in only two samples.
Conclusions
We identified a number of sapovirus and AiV‐1 strains belonging to multiple genotypes in wastewater samples collected over a 13‐month period. Our results suggested a temporal shift in prevalent genotypes in the community.
Significance and Impact of the Study
This is the first study elucidating the genotype distribution of human sapoviruses and AiV‐1 in wastewater in the United States. Wastewater surveillance is especially useful for understanding molecular epidemiology of viruses that are less commonly tested in clinical diagnosis, including sapoviruses and AiV‐1. |
| doi_str_mv | 10.1111/jam.13712 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1993010740</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1993010740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4982-bc076ed5755bc2adab1839482e6feec402551e6fc811b9ddd676abae7242d6673</originalsourceid><addsrcrecordid>eNp1kU1v1DAQhi0Eoh9w4A8gS1yoRFrbie3NcVXxqSIObc_WJJ5lvUrsYDtdLWd-ON7dwgEJX-bDz7wz0kvIK84ueXlXGxgvea25eEJOea1kJZQWTw95U0mmxQk5S2nDGK-ZVM_JiWhrrSUTp-TXHY5TiDDQB4gOsgs-Uefpd_Qh7yak1qUcXTfvf2hY0fU8gqcJpvDg4pwwUfCWLl2_dvTQoXw_v4WUcQsZ4766DXNeY_R0Gd3P4OEdvfcuo6W3uSDpBXm2giHhy8d4Tu4_vL-7_lTdfPv4-Xp5U_VNuxBV1zOt0EotZdcLsNDxRd02C4Fqhdg3TEjJS94vOO9aa63SCjpALRphldL1OXl71J1i-DFjymZ0qcdhAI9hToa3bc040w0r6Jt_0E2Yoy_XGVH21FwIzQt1caT6GFKKuDJTdCPEneHM7K0xxRpzsKawrx8V525E-5f840UBro7A1g24-7-S-bL8epT8Ddx6mZM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2025312271</pqid></control><display><type>article</type><title>Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Kitajima, M. ; Rachmadi, A.T. ; Iker, B.C. ; Haramoto, E. ; Gerba, C.P.</creator><creatorcontrib>Kitajima, M. ; Rachmadi, A.T. ; Iker, B.C. ; Haramoto, E. ; Gerba, C.P.</creatorcontrib><description>Aims
To investigate the molecular epidemiology, especially temporal variations in genotype distribution, of sapoviruses and Aichi virus 1 (AiV‐1) in Arizona, United States, by examining wastewater.
Methods and Results
A total of 26 wastewater samples (13 influent and 13 effluent) were collected monthly from a wastewater treatment plant and viral strains were identified through nested reverse transcription‐PCR followed by cloning and sequencing analysis. Identified sapovirus strains were classified into seven genotypes belonging to three genogroups (GI, GII, and GV): GI.1, GI.2, GI.3, GII.1, GII.2, GII.8 and GV.1, with a clear temporal shift. The majority of AiV‐1 strains identified from the wastewater samples were classified into genotype B, and genotype A strains were identified in only two samples.
Conclusions
We identified a number of sapovirus and AiV‐1 strains belonging to multiple genotypes in wastewater samples collected over a 13‐month period. Our results suggested a temporal shift in prevalent genotypes in the community.
Significance and Impact of the Study
This is the first study elucidating the genotype distribution of human sapoviruses and AiV‐1 in wastewater in the United States. Wastewater surveillance is especially useful for understanding molecular epidemiology of viruses that are less commonly tested in clinical diagnosis, including sapoviruses and AiV‐1.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13712</identifier><identifier>PMID: 29377502</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Aichi virus 1 ; Cloning ; Epidemiology ; gastroenteritis ; Genotypes ; genotyping ; molecular epidemiology ; PCR ; Reverse transcription ; sapovirus ; sequencing ; Strains (organisms) ; Temporal variations ; Viruses ; Wastewater treatment ; Wastewater treatment plants ; water</subject><ispartof>Journal of applied microbiology, 2018-05, Vol.124 (5), p.1324-1332</ispartof><rights>2018 The Society for Applied Microbiology</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>Copyright © 2018 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4982-bc076ed5755bc2adab1839482e6feec402551e6fc811b9ddd676abae7242d6673</citedby><cites>FETCH-LOGICAL-c4982-bc076ed5755bc2adab1839482e6feec402551e6fc811b9ddd676abae7242d6673</cites><orcidid>0000-0002-8142-511X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.13712$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.13712$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29377502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitajima, M.</creatorcontrib><creatorcontrib>Rachmadi, A.T.</creatorcontrib><creatorcontrib>Iker, B.C.</creatorcontrib><creatorcontrib>Haramoto, E.</creatorcontrib><creatorcontrib>Gerba, C.P.</creatorcontrib><title>Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To investigate the molecular epidemiology, especially temporal variations in genotype distribution, of sapoviruses and Aichi virus 1 (AiV‐1) in Arizona, United States, by examining wastewater.
Methods and Results
A total of 26 wastewater samples (13 influent and 13 effluent) were collected monthly from a wastewater treatment plant and viral strains were identified through nested reverse transcription‐PCR followed by cloning and sequencing analysis. Identified sapovirus strains were classified into seven genotypes belonging to three genogroups (GI, GII, and GV): GI.1, GI.2, GI.3, GII.1, GII.2, GII.8 and GV.1, with a clear temporal shift. The majority of AiV‐1 strains identified from the wastewater samples were classified into genotype B, and genotype A strains were identified in only two samples.
Conclusions
We identified a number of sapovirus and AiV‐1 strains belonging to multiple genotypes in wastewater samples collected over a 13‐month period. Our results suggested a temporal shift in prevalent genotypes in the community.
Significance and Impact of the Study
This is the first study elucidating the genotype distribution of human sapoviruses and AiV‐1 in wastewater in the United States. Wastewater surveillance is especially useful for understanding molecular epidemiology of viruses that are less commonly tested in clinical diagnosis, including sapoviruses and AiV‐1.</description><subject>Aichi virus 1</subject><subject>Cloning</subject><subject>Epidemiology</subject><subject>gastroenteritis</subject><subject>Genotypes</subject><subject>genotyping</subject><subject>molecular epidemiology</subject><subject>PCR</subject><subject>Reverse transcription</subject><subject>sapovirus</subject><subject>sequencing</subject><subject>Strains (organisms)</subject><subject>Temporal variations</subject><subject>Viruses</subject><subject>Wastewater treatment</subject><subject>Wastewater treatment plants</subject><subject>water</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kU1v1DAQhi0Eoh9w4A8gS1yoRFrbie3NcVXxqSIObc_WJJ5lvUrsYDtdLWd-ON7dwgEJX-bDz7wz0kvIK84ueXlXGxgvea25eEJOea1kJZQWTw95U0mmxQk5S2nDGK-ZVM_JiWhrrSUTp-TXHY5TiDDQB4gOsgs-Uefpd_Qh7yak1qUcXTfvf2hY0fU8gqcJpvDg4pwwUfCWLl2_dvTQoXw_v4WUcQsZ4766DXNeY_R0Gd3P4OEdvfcuo6W3uSDpBXm2giHhy8d4Tu4_vL-7_lTdfPv4-Xp5U_VNuxBV1zOt0EotZdcLsNDxRd02C4Fqhdg3TEjJS94vOO9aa63SCjpALRphldL1OXl71J1i-DFjymZ0qcdhAI9hToa3bc040w0r6Jt_0E2Yoy_XGVH21FwIzQt1caT6GFKKuDJTdCPEneHM7K0xxRpzsKawrx8V525E-5f840UBro7A1g24-7-S-bL8epT8Ddx6mZM</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Kitajima, M.</creator><creator>Rachmadi, A.T.</creator><creator>Iker, B.C.</creator><creator>Haramoto, E.</creator><creator>Gerba, C.P.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8142-511X</orcidid></search><sort><creationdate>201805</creationdate><title>Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States</title><author>Kitajima, M. ; Rachmadi, A.T. ; Iker, B.C. ; Haramoto, E. ; Gerba, C.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4982-bc076ed5755bc2adab1839482e6feec402551e6fc811b9ddd676abae7242d6673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aichi virus 1</topic><topic>Cloning</topic><topic>Epidemiology</topic><topic>gastroenteritis</topic><topic>Genotypes</topic><topic>genotyping</topic><topic>molecular epidemiology</topic><topic>PCR</topic><topic>Reverse transcription</topic><topic>sapovirus</topic><topic>sequencing</topic><topic>Strains (organisms)</topic><topic>Temporal variations</topic><topic>Viruses</topic><topic>Wastewater treatment</topic><topic>Wastewater treatment plants</topic><topic>water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitajima, M.</creatorcontrib><creatorcontrib>Rachmadi, A.T.</creatorcontrib><creatorcontrib>Iker, B.C.</creatorcontrib><creatorcontrib>Haramoto, E.</creatorcontrib><creatorcontrib>Gerba, C.P.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitajima, M.</au><au>Rachmadi, A.T.</au><au>Iker, B.C.</au><au>Haramoto, E.</au><au>Gerba, C.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2018-05</date><risdate>2018</risdate><volume>124</volume><issue>5</issue><spage>1324</spage><epage>1332</epage><pages>1324-1332</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
To investigate the molecular epidemiology, especially temporal variations in genotype distribution, of sapoviruses and Aichi virus 1 (AiV‐1) in Arizona, United States, by examining wastewater.
Methods and Results
A total of 26 wastewater samples (13 influent and 13 effluent) were collected monthly from a wastewater treatment plant and viral strains were identified through nested reverse transcription‐PCR followed by cloning and sequencing analysis. Identified sapovirus strains were classified into seven genotypes belonging to three genogroups (GI, GII, and GV): GI.1, GI.2, GI.3, GII.1, GII.2, GII.8 and GV.1, with a clear temporal shift. The majority of AiV‐1 strains identified from the wastewater samples were classified into genotype B, and genotype A strains were identified in only two samples.
Conclusions
We identified a number of sapovirus and AiV‐1 strains belonging to multiple genotypes in wastewater samples collected over a 13‐month period. Our results suggested a temporal shift in prevalent genotypes in the community.
Significance and Impact of the Study
This is the first study elucidating the genotype distribution of human sapoviruses and AiV‐1 in wastewater in the United States. Wastewater surveillance is especially useful for understanding molecular epidemiology of viruses that are less commonly tested in clinical diagnosis, including sapoviruses and AiV‐1.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29377502</pmid><doi>10.1111/jam.13712</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8142-511X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1364-5072 |
| ispartof | Journal of applied microbiology, 2018-05, Vol.124 (5), p.1324-1332 |
| issn | 1364-5072 1365-2672 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1993010740 |
| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | Aichi virus 1 Cloning Epidemiology gastroenteritis Genotypes genotyping molecular epidemiology PCR Reverse transcription sapovirus sequencing Strains (organisms) Temporal variations Viruses Wastewater treatment Wastewater treatment plants water |
| title | Temporal variations in genotype distribution of human sapoviruses and Aichi virus 1 in wastewater in Southern Arizona, United States |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T07%3A56%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Temporal%20variations%20in%20genotype%20distribution%20of%20human%20sapoviruses%20and%20Aichi%20virus%201%20in%20wastewater%20in%20Southern%20Arizona,%20United%20States&rft.jtitle=Journal%20of%20applied%20microbiology&rft.au=Kitajima,%20M.&rft.date=2018-05&rft.volume=124&rft.issue=5&rft.spage=1324&rft.epage=1332&rft.pages=1324-1332&rft.issn=1364-5072&rft.eissn=1365-2672&rft_id=info:doi/10.1111/jam.13712&rft_dat=%3Cproquest_cross%3E1993010740%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2025312271&rft_id=info:pmid/29377502&rfr_iscdi=true |