A seminested PCR assay for detection and typing of human papillomavirus based on E1 gene sequences

HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing is critical for defining the prognosis of cancer. In this work, a seminested PCR which allow fast and inexpensively detection and typing of HPV is presented. The system is based on the amplification of a variable length region within the viral gene E1, using three primers that potentially anneal in all HPV genomes. The amplicons produced in the first step can be identified by high resolution electrophoresis or direct sequencing. The seminested step includes nine specific primers which can be used in multiplex or individual reactions to discriminate the main types of HPV by amplicon size differentiation using agarose electrophoresis, reducing the time spent and cost per analysis. •A region within E1 ORF varies in length according to the type of HPV.•A new PCR protocol for HPV detection and typing was developed.•The semi-nested reaction allows differentiation of HPVs by agarose electrophoresis.•This is a suitable method for use in low resource settings.