Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants
A promoter is an essential structural component of a gene that controls its transcription activity in different development stages and in response to various environmental stimuli. Knowledge of promoter functionality in heterologous systems is important in the study of gene regulation and biotechnol...
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| Veröffentlicht in: | Molecular biotechnology 2018-03, Vol.60 (3), p.194-202 |
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| creator | Kirke, Justin Kaplan, Noah Velez, Stephanie Jin, Xiao-Lu Vichyavichien, Paveena Zhang, Xing-Hai |
| description | A promoter is an essential structural component of a gene that controls its transcription activity in different development stages and in response to various environmental stimuli. Knowledge of promoter functionality in heterologous systems is important in the study of gene regulation and biotechnological application. In order to explore the activity of the pepper capsaicin synthase gene (
PUN1
) promoter, gene constructs of
pPUN1::GUS
(for β-glucuronidase) and
pPUN1::NtKED
(for a tobacco wound-responsive protein) were introduced into tobacco and tomato, respectively, and their activities were examined. Higher levels of GUS staining intensity and transcription were detected in ovary, anther and pollen than other tissues or organs in tobacco plants. Likewise, transgenic tomato fruits had a higher level of
pPUN1::NtKED
gene expression than the leaf and flower. The
PUN1
-driven gene expression can be transiently induced by wounding, heat (40 °C) and the capsaicinoid biosynthetic pathway precursor phenylalanine. When compared to the reported
pPUN1::GUS
-expressing
Arabidopsis
, the
PUN1
promoter exhibited a more similar pattern of activities among pepper, tobacco and tomato, all
Solanaceae
plants. Our results suggest the potential utility of this tissue-preferential and inducible promoter in other non-pungent
Solanaceae
plants for research of gene function and regulation as well as in the biotechnological applications. |
| doi_str_mv | 10.1007/s12033-018-0060-0 |
| format | Article |
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PUN1
) promoter, gene constructs of
pPUN1::GUS
(for β-glucuronidase) and
pPUN1::NtKED
(for a tobacco wound-responsive protein) were introduced into tobacco and tomato, respectively, and their activities were examined. Higher levels of GUS staining intensity and transcription were detected in ovary, anther and pollen than other tissues or organs in tobacco plants. Likewise, transgenic tomato fruits had a higher level of
pPUN1::NtKED
gene expression than the leaf and flower. The
PUN1
-driven gene expression can be transiently induced by wounding, heat (40 °C) and the capsaicinoid biosynthetic pathway precursor phenylalanine. When compared to the reported
pPUN1::GUS
-expressing
Arabidopsis
, the
PUN1
promoter exhibited a more similar pattern of activities among pepper, tobacco and tomato, all
Solanaceae
plants. Our results suggest the potential utility of this tissue-preferential and inducible promoter in other non-pungent
Solanaceae
plants for research of gene function and regulation as well as in the biotechnological applications.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-018-0060-0</identifier><identifier>PMID: 29372506</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biological Techniques ; Biotechnology ; Capsaicin ; Capsaicin - metabolism ; Capsicum - enzymology ; Cell Biology ; Chemistry ; Chemistry and Materials Science ; Developmental stages ; Environmental effects ; Fruit - genetics ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Glucuronidase - metabolism ; Hot Temperature ; Human Genetics ; Metabolic Networks and Pathways ; Nicotiana - genetics ; Organ Specificity ; Organs ; Original Paper ; Phenylalanine ; Plant Proteins - genetics ; Plants, Genetically Modified ; Pollen ; Precursors ; Promoter Regions, Genetic ; Protein Science ; Solanaceae ; Solanum lycopersicum - genetics ; Stress, Physiological ; Tobacco ; Tomatoes ; Transcription ; Transcription, Genetic ; Transformation, Genetic ; Transgenes ; Transgenic plants ; Wounding</subject><ispartof>Molecular biotechnology, 2018-03, Vol.60 (3), p.194-202</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Molecular Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-1675997c06f70bd057e57668f994283a642d69352405f3aeb77dc6626d1925b03</citedby><cites>FETCH-LOGICAL-c398t-1675997c06f70bd057e57668f994283a642d69352405f3aeb77dc6626d1925b03</cites><orcidid>0000-0001-9261-4242</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12033-018-0060-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12033-018-0060-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29372506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kirke, Justin</creatorcontrib><creatorcontrib>Kaplan, Noah</creatorcontrib><creatorcontrib>Velez, Stephanie</creatorcontrib><creatorcontrib>Jin, Xiao-Lu</creatorcontrib><creatorcontrib>Vichyavichien, Paveena</creatorcontrib><creatorcontrib>Zhang, Xing-Hai</creatorcontrib><title>Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><addtitle>Mol Biotechnol</addtitle><description>A promoter is an essential structural component of a gene that controls its transcription activity in different development stages and in response to various environmental stimuli. Knowledge of promoter functionality in heterologous systems is important in the study of gene regulation and biotechnological application. In order to explore the activity of the pepper capsaicin synthase gene (
PUN1
) promoter, gene constructs of
pPUN1::GUS
(for β-glucuronidase) and
pPUN1::NtKED
(for a tobacco wound-responsive protein) were introduced into tobacco and tomato, respectively, and their activities were examined. Higher levels of GUS staining intensity and transcription were detected in ovary, anther and pollen than other tissues or organs in tobacco plants. Likewise, transgenic tomato fruits had a higher level of
pPUN1::NtKED
gene expression than the leaf and flower. The
PUN1
-driven gene expression can be transiently induced by wounding, heat (40 °C) and the capsaicinoid biosynthetic pathway precursor phenylalanine. When compared to the reported
pPUN1::GUS
-expressing
Arabidopsis
, the
PUN1
promoter exhibited a more similar pattern of activities among pepper, tobacco and tomato, all
Solanaceae
plants. Our results suggest the potential utility of this tissue-preferential and inducible promoter in other non-pungent
Solanaceae
plants for research of gene function and regulation as well as in the biotechnological applications.</description><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biotechnology</subject><subject>Capsaicin</subject><subject>Capsaicin - metabolism</subject><subject>Capsicum - enzymology</subject><subject>Cell Biology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Developmental stages</subject><subject>Environmental effects</subject><subject>Fruit - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Glucuronidase - metabolism</subject><subject>Hot Temperature</subject><subject>Human Genetics</subject><subject>Metabolic Networks and Pathways</subject><subject>Nicotiana - genetics</subject><subject>Organ Specificity</subject><subject>Organs</subject><subject>Original Paper</subject><subject>Phenylalanine</subject><subject>Plant Proteins - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Pollen</subject><subject>Precursors</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Science</subject><subject>Solanaceae</subject><subject>Solanum lycopersicum - genetics</subject><subject>Stress, Physiological</subject><subject>Tobacco</subject><subject>Tomatoes</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><subject>Transformation, Genetic</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>Wounding</subject><issn>1073-6085</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFu1DAQhiMEoqXwAFyQJS4cCIzt2I6P1QpopdJGYiuOkeM4XVeJHWynVd6Ix8TbLQghcRqP5pt_fvkvitcYPmAA8TFiApSWgOsSgEMJT4pjzJgsgQJ7mt8gaMmhZkfFixhvAQhmFX1eHBFJBWHAj4ufWxvjYsommMEE45JVIzrVyd7ZtCLlenTu-iX33iE_oLQzqDHzbALaqDkqq61D31aXdirmyfUlRk3wk08Z6Fb03S-ut-7mPTozKj3IfTVJdX60GjUq7e7VmheMXkL0AWWtre-U1v4B3fpJJY-aUbkUXxbPBjVG8-qxnhTXnz9tN2flxdWX883pRamprFOJuWBSCg18END1wIRhgvN6kLIiNVW8Ij2XlJEK2ECV6YToNeeE91gS1gE9Kd4ddOfgfywmpnayUZsxmzB-iS2WkgAmVY0z-vYf9NYvwWV3e4pW-TDmmcIHSgcfY_7mdg52UmFtMbT7GNtDjG2Osd3H2O5NvHlUXrrJ9H82fueWAXIAYh65GxP-Ov1f1V9ZxqeO</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Kirke, Justin</creator><creator>Kaplan, Noah</creator><creator>Velez, Stephanie</creator><creator>Jin, Xiao-Lu</creator><creator>Vichyavichien, Paveena</creator><creator>Zhang, Xing-Hai</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9261-4242</orcidid></search><sort><creationdate>20180301</creationdate><title>Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants</title><author>Kirke, Justin ; Kaplan, Noah ; Velez, Stephanie ; Jin, Xiao-Lu ; Vichyavichien, Paveena ; Zhang, Xing-Hai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-1675997c06f70bd057e57668f994283a642d69352405f3aeb77dc6626d1925b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Biochemistry</topic><topic>Biological Techniques</topic><topic>Biotechnology</topic><topic>Capsaicin</topic><topic>Capsaicin - metabolism</topic><topic>Capsicum - enzymology</topic><topic>Cell Biology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Developmental stages</topic><topic>Environmental effects</topic><topic>Fruit - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Glucuronidase - metabolism</topic><topic>Hot Temperature</topic><topic>Human Genetics</topic><topic>Metabolic Networks and Pathways</topic><topic>Nicotiana - genetics</topic><topic>Organ Specificity</topic><topic>Organs</topic><topic>Original Paper</topic><topic>Phenylalanine</topic><topic>Plant Proteins - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Pollen</topic><topic>Precursors</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Science</topic><topic>Solanaceae</topic><topic>Solanum lycopersicum - genetics</topic><topic>Stress, Physiological</topic><topic>Tobacco</topic><topic>Tomatoes</topic><topic>Transcription</topic><topic>Transcription, Genetic</topic><topic>Transformation, Genetic</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>Wounding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kirke, Justin</creatorcontrib><creatorcontrib>Kaplan, Noah</creatorcontrib><creatorcontrib>Velez, Stephanie</creatorcontrib><creatorcontrib>Jin, Xiao-Lu</creatorcontrib><creatorcontrib>Vichyavichien, Paveena</creatorcontrib><creatorcontrib>Zhang, Xing-Hai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirke, Justin</au><au>Kaplan, Noah</au><au>Velez, Stephanie</au><au>Jin, Xiao-Lu</au><au>Vichyavichien, Paveena</au><au>Zhang, Xing-Hai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>60</volume><issue>3</issue><spage>194</spage><epage>202</epage><pages>194-202</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><abstract>A promoter is an essential structural component of a gene that controls its transcription activity in different development stages and in response to various environmental stimuli. Knowledge of promoter functionality in heterologous systems is important in the study of gene regulation and biotechnological application. In order to explore the activity of the pepper capsaicin synthase gene (
PUN1
) promoter, gene constructs of
pPUN1::GUS
(for β-glucuronidase) and
pPUN1::NtKED
(for a tobacco wound-responsive protein) were introduced into tobacco and tomato, respectively, and their activities were examined. Higher levels of GUS staining intensity and transcription were detected in ovary, anther and pollen than other tissues or organs in tobacco plants. Likewise, transgenic tomato fruits had a higher level of
pPUN1::NtKED
gene expression than the leaf and flower. The
PUN1
-driven gene expression can be transiently induced by wounding, heat (40 °C) and the capsaicinoid biosynthetic pathway precursor phenylalanine. When compared to the reported
pPUN1::GUS
-expressing
Arabidopsis
, the
PUN1
promoter exhibited a more similar pattern of activities among pepper, tobacco and tomato, all
Solanaceae
plants. Our results suggest the potential utility of this tissue-preferential and inducible promoter in other non-pungent
Solanaceae
plants for research of gene function and regulation as well as in the biotechnological applications.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29372506</pmid><doi>10.1007/s12033-018-0060-0</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9261-4242</orcidid></addata></record> |
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| ispartof | Molecular biotechnology, 2018-03, Vol.60 (3), p.194-202 |
| issn | 1073-6085 1559-0305 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Biochemistry Biological Techniques Biotechnology Capsaicin Capsaicin - metabolism Capsicum - enzymology Cell Biology Chemistry Chemistry and Materials Science Developmental stages Environmental effects Fruit - genetics Gene expression Gene Expression Regulation, Plant Gene regulation Glucuronidase - metabolism Hot Temperature Human Genetics Metabolic Networks and Pathways Nicotiana - genetics Organ Specificity Organs Original Paper Phenylalanine Plant Proteins - genetics Plants, Genetically Modified Pollen Precursors Promoter Regions, Genetic Protein Science Solanaceae Solanum lycopersicum - genetics Stress, Physiological Tobacco Tomatoes Transcription Transcription, Genetic Transformation, Genetic Transgenes Transgenic plants Wounding |
| title | Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants |
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