Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies
Background Distinct cytokine expression patterns have been reported in biomaterial of patients with polyneuropathies (PNP). We investigated gene expression profiles of pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) of patients with neuropathies of different etiolog...
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| Veröffentlicht in: | Journal of neurology 2018-03, Vol.265 (3), p.618-627 |
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| creator | Langjahr, Melissa Schubert, Anna-Lena Sommer, Claudia Üçeyler, Nurcan |
| description | Background
Distinct cytokine expression patterns have been reported in biomaterial of patients with polyneuropathies (PNP). We investigated gene expression profiles of pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) of patients with neuropathies of different etiologies.
Methods
We prospectively studied 97 patients with neuropathies and compared data between diagnostic subgroups and healthy controls. Gene expression of a panel of pro- and anti-inflammatory cytokines was analyzed (interleukin-1 [IL-1], IL-2, IL-6, IL-8, tumor necrosis factor alpha [TNF], IL-4, and IL-10) in PBMC samples. Furthermore, protein levels of IL-6, IL-8, and TNF were measured in supernatant of PBMC stimulated with lipopolysaccharide (LPS).
Results
PNP were associated with higher PBMC gene expression of IL-1 (
p
|
| doi_str_mv | 10.1007/s00415-018-8748-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1992012332</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1993455764</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-213cc1af305eaac56ee5a448526b38a1a62445e46c0793b2d5e10bc7fe88e6a43</originalsourceid><addsrcrecordid>eNp1kU1r3DAQhkVoSDYfPyCXIuilFyX6tOVjCW0TCPSSnIWsHe9qa0uuZNMs_fOR2TSUQC8SaJ55Z8SD0BWj14zS-iZTKpkilGmia6mJPEIrJgUnTKrmA1pRISlRQslTdJbzjlKqS-EEnfJG1FxovUJ_7oNLYDOs8Zgi8aHr7TDYKaY9dvsp_vQB8AbKAc9jgpx9DNgHPELy4xaS7XHbx7jGQwwxzK4Hm7CDvs84dni0k4cwZfzbT1s8xn4fYE6xPG895At03Nk-w-XrfY6evn19vL0jDz--399-eSBONHoinAnnmO0EVWCtUxWAslJqxatWaMtsxaVUICtH60a0fK2A0dbVHWgNlZXiHH0-5JYf_pohT2bwednRBohzNqxpOGVcCF7QT-_QXZxTKNstlJBK1dUSyA6USzHnBJ0Zkx9s2htGzWLGHMyYYsYsZszS8_E1eW4HWL91_FVRAH4AcimFDaR_Rv839QWA7Ju6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993455764</pqid></control><display><type>article</type><title>Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Langjahr, Melissa ; Schubert, Anna-Lena ; Sommer, Claudia ; Üçeyler, Nurcan</creator><creatorcontrib>Langjahr, Melissa ; Schubert, Anna-Lena ; Sommer, Claudia ; Üçeyler, Nurcan</creatorcontrib><description><![CDATA[Background
Distinct cytokine expression patterns have been reported in biomaterial of patients with polyneuropathies (PNP). We investigated gene expression profiles of pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) of patients with neuropathies of different etiologies.
Methods
We prospectively studied 97 patients with neuropathies and compared data between diagnostic subgroups and healthy controls. Gene expression of a panel of pro- and anti-inflammatory cytokines was analyzed (interleukin-1 [IL-1], IL-2, IL-6, IL-8, tumor necrosis factor alpha [TNF], IL-4, and IL-10) in PBMC samples. Furthermore, protein levels of IL-6, IL-8, and TNF were measured in supernatant of PBMC stimulated with lipopolysaccharide (LPS).
Results
PNP were associated with higher PBMC gene expression of IL-1 (
p
< 0.05), IL-2 (
p
< 0.05), IL-8 (
p
< 0.001), and TNF (
p
< 0.01) compared to healthy controls. Inflammatory neuropathies were associated with higher gene expression of IL-8 (
p
< 0.001) and TNF (
p
< 0.05) and lower gene expression of IL-10 (
p
< 0.05) compared to healthy controls. More pro-inflammatory cytokines were elevated in painful neuropathy (IL-1, IL-2 [
p
< 0.05], IL-8 [
p
< 0.001] and TNF [
p
< 0.05]) than in painless neuropathy (IL-8 [
p
< 0.01] and TNF [
p
< 0.01]) compared to healthy controls, while IL-10 expression was higher in treatment naïve patients with painless neuropathy compared to patients with painful neuropathy (
p
< 0.05). Disease duration positively correlated with IL-6 gene expression (
p
< 0.01). Supernatant protein levels of IL-6, IL-8, and TNF did not differ between groups.
Conclusion
Systemic gene expression of pro-inflammatory cytokines is increased in patients with neuropathies and may be influenced by the presence of neuropathic pain.]]></description><identifier>ISSN: 0340-5354</identifier><identifier>EISSN: 1432-1459</identifier><identifier>DOI: 10.1007/s00415-018-8748-4</identifier><identifier>PMID: 29372388</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biomaterials ; Cytokines ; Cytokines - blood ; Female ; Gene Expression ; Humans ; Inflammation ; Interleukin 1 ; Interleukin 10 ; Interleukin 2 ; Interleukin 4 ; Interleukin 6 ; Interleukin 8 ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - metabolism ; Lipopolysaccharides ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Neuralgia - blood ; Neuralgia - immunology ; Neurology ; Neuroradiology ; Neurosciences ; Original Communication ; Peripheral blood mononuclear cells ; Peripheral neuropathy ; Polyneuropathies - blood ; Polyneuropathies - immunology ; Prospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Young Adult</subject><ispartof>Journal of neurology, 2018-03, Vol.265 (3), p.618-627</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Journal of Neurology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-213cc1af305eaac56ee5a448526b38a1a62445e46c0793b2d5e10bc7fe88e6a43</citedby><cites>FETCH-LOGICAL-c398t-213cc1af305eaac56ee5a448526b38a1a62445e46c0793b2d5e10bc7fe88e6a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00415-018-8748-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00415-018-8748-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29372388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Langjahr, Melissa</creatorcontrib><creatorcontrib>Schubert, Anna-Lena</creatorcontrib><creatorcontrib>Sommer, Claudia</creatorcontrib><creatorcontrib>Üçeyler, Nurcan</creatorcontrib><title>Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies</title><title>Journal of neurology</title><addtitle>J Neurol</addtitle><addtitle>J Neurol</addtitle><description><![CDATA[Background
Distinct cytokine expression patterns have been reported in biomaterial of patients with polyneuropathies (PNP). We investigated gene expression profiles of pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) of patients with neuropathies of different etiologies.
Methods
We prospectively studied 97 patients with neuropathies and compared data between diagnostic subgroups and healthy controls. Gene expression of a panel of pro- and anti-inflammatory cytokines was analyzed (interleukin-1 [IL-1], IL-2, IL-6, IL-8, tumor necrosis factor alpha [TNF], IL-4, and IL-10) in PBMC samples. Furthermore, protein levels of IL-6, IL-8, and TNF were measured in supernatant of PBMC stimulated with lipopolysaccharide (LPS).
Results
PNP were associated with higher PBMC gene expression of IL-1 (
p
< 0.05), IL-2 (
p
< 0.05), IL-8 (
p
< 0.001), and TNF (
p
< 0.01) compared to healthy controls. Inflammatory neuropathies were associated with higher gene expression of IL-8 (
p
< 0.001) and TNF (
p
< 0.05) and lower gene expression of IL-10 (
p
< 0.05) compared to healthy controls. More pro-inflammatory cytokines were elevated in painful neuropathy (IL-1, IL-2 [
p
< 0.05], IL-8 [
p
< 0.001] and TNF [
p
< 0.05]) than in painless neuropathy (IL-8 [
p
< 0.01] and TNF [
p
< 0.01]) compared to healthy controls, while IL-10 expression was higher in treatment naïve patients with painless neuropathy compared to patients with painful neuropathy (
p
< 0.05). Disease duration positively correlated with IL-6 gene expression (
p
< 0.01). Supernatant protein levels of IL-6, IL-8, and TNF did not differ between groups.
Conclusion
Systemic gene expression of pro-inflammatory cytokines is increased in patients with neuropathies and may be influenced by the presence of neuropathic pain.]]></description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomaterials</subject><subject>Cytokines</subject><subject>Cytokines - blood</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Interleukin 1</subject><subject>Interleukin 10</subject><subject>Interleukin 2</subject><subject>Interleukin 4</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lipopolysaccharides</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neuralgia - blood</subject><subject>Neuralgia - immunology</subject><subject>Neurology</subject><subject>Neuroradiology</subject><subject>Neurosciences</subject><subject>Original Communication</subject><subject>Peripheral blood mononuclear cells</subject><subject>Peripheral neuropathy</subject><subject>Polyneuropathies - blood</subject><subject>Polyneuropathies - immunology</subject><subject>Prospective Studies</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Young Adult</subject><issn>0340-5354</issn><issn>1432-1459</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kU1r3DAQhkVoSDYfPyCXIuilFyX6tOVjCW0TCPSSnIWsHe9qa0uuZNMs_fOR2TSUQC8SaJ55Z8SD0BWj14zS-iZTKpkilGmia6mJPEIrJgUnTKrmA1pRISlRQslTdJbzjlKqS-EEnfJG1FxovUJ_7oNLYDOs8Zgi8aHr7TDYKaY9dvsp_vQB8AbKAc9jgpx9DNgHPELy4xaS7XHbx7jGQwwxzK4Hm7CDvs84dni0k4cwZfzbT1s8xn4fYE6xPG895At03Nk-w-XrfY6evn19vL0jDz--399-eSBONHoinAnnmO0EVWCtUxWAslJqxatWaMtsxaVUICtH60a0fK2A0dbVHWgNlZXiHH0-5JYf_pohT2bwednRBohzNqxpOGVcCF7QT-_QXZxTKNstlJBK1dUSyA6USzHnBJ0Zkx9s2htGzWLGHMyYYsYsZszS8_E1eW4HWL91_FVRAH4AcimFDaR_Rv839QWA7Ju6</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Langjahr, Melissa</creator><creator>Schubert, Anna-Lena</creator><creator>Sommer, Claudia</creator><creator>Üçeyler, Nurcan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20180301</creationdate><title>Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies</title><author>Langjahr, Melissa ; Schubert, Anna-Lena ; Sommer, Claudia ; Üçeyler, Nurcan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-213cc1af305eaac56ee5a448526b38a1a62445e46c0793b2d5e10bc7fe88e6a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomaterials</topic><topic>Cytokines</topic><topic>Cytokines - blood</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Interleukin 1</topic><topic>Interleukin 10</topic><topic>Interleukin 2</topic><topic>Interleukin 4</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Leukocytes (mononuclear)</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lipopolysaccharides</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Neuralgia - blood</topic><topic>Neuralgia - immunology</topic><topic>Neurology</topic><topic>Neuroradiology</topic><topic>Neurosciences</topic><topic>Original Communication</topic><topic>Peripheral blood mononuclear cells</topic><topic>Peripheral neuropathy</topic><topic>Polyneuropathies - blood</topic><topic>Polyneuropathies - immunology</topic><topic>Prospective Studies</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Langjahr, Melissa</creatorcontrib><creatorcontrib>Schubert, Anna-Lena</creatorcontrib><creatorcontrib>Sommer, Claudia</creatorcontrib><creatorcontrib>Üçeyler, Nurcan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Langjahr, Melissa</au><au>Schubert, Anna-Lena</au><au>Sommer, Claudia</au><au>Üçeyler, Nurcan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies</atitle><jtitle>Journal of neurology</jtitle><stitle>J Neurol</stitle><addtitle>J Neurol</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>265</volume><issue>3</issue><spage>618</spage><epage>627</epage><pages>618-627</pages><issn>0340-5354</issn><eissn>1432-1459</eissn><abstract><![CDATA[Background
Distinct cytokine expression patterns have been reported in biomaterial of patients with polyneuropathies (PNP). We investigated gene expression profiles of pro- and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMC) of patients with neuropathies of different etiologies.
Methods
We prospectively studied 97 patients with neuropathies and compared data between diagnostic subgroups and healthy controls. Gene expression of a panel of pro- and anti-inflammatory cytokines was analyzed (interleukin-1 [IL-1], IL-2, IL-6, IL-8, tumor necrosis factor alpha [TNF], IL-4, and IL-10) in PBMC samples. Furthermore, protein levels of IL-6, IL-8, and TNF were measured in supernatant of PBMC stimulated with lipopolysaccharide (LPS).
Results
PNP were associated with higher PBMC gene expression of IL-1 (
p
< 0.05), IL-2 (
p
< 0.05), IL-8 (
p
< 0.001), and TNF (
p
< 0.01) compared to healthy controls. Inflammatory neuropathies were associated with higher gene expression of IL-8 (
p
< 0.001) and TNF (
p
< 0.05) and lower gene expression of IL-10 (
p
< 0.05) compared to healthy controls. More pro-inflammatory cytokines were elevated in painful neuropathy (IL-1, IL-2 [
p
< 0.05], IL-8 [
p
< 0.001] and TNF [
p
< 0.05]) than in painless neuropathy (IL-8 [
p
< 0.01] and TNF [
p
< 0.01]) compared to healthy controls, while IL-10 expression was higher in treatment naïve patients with painless neuropathy compared to patients with painful neuropathy (
p
< 0.05). Disease duration positively correlated with IL-6 gene expression (
p
< 0.01). Supernatant protein levels of IL-6, IL-8, and TNF did not differ between groups.
Conclusion
Systemic gene expression of pro-inflammatory cytokines is increased in patients with neuropathies and may be influenced by the presence of neuropathic pain.]]></abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29372388</pmid><doi>10.1007/s00415-018-8748-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-5354 |
| ispartof | Journal of neurology, 2018-03, Vol.265 (3), p.618-627 |
| issn | 0340-5354 1432-1459 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Biomaterials Cytokines Cytokines - blood Female Gene Expression Humans Inflammation Interleukin 1 Interleukin 10 Interleukin 2 Interleukin 4 Interleukin 6 Interleukin 8 Leukocytes (mononuclear) Leukocytes, Mononuclear - metabolism Lipopolysaccharides Male Medicine Medicine & Public Health Middle Aged Neuralgia - blood Neuralgia - immunology Neurology Neuroradiology Neurosciences Original Communication Peripheral blood mononuclear cells Peripheral neuropathy Polyneuropathies - blood Polyneuropathies - immunology Prospective Studies Reverse Transcriptase Polymerase Chain Reaction Tumor necrosis factor-TNF Tumor necrosis factor-α Young Adult |
| title | Increased pro-inflammatory cytokine gene expression in peripheral blood mononuclear cells of patients with polyneuropathies |
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