Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters
Aims: An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters. Methods and Results: The fluorogenic substrate 4‐methylumbelliferyl‐β‐d‐glucuronide is cleaved...
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| Veröffentlicht in: | Journal of applied microbiology 2002-01, Vol.93 (4), p.548-556 |
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| creator | Caruso, G. Crisafi, E. Mancuso, M. |
| description | Aims: An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters.
Methods and Results: The fluorogenic substrate 4‐methylumbelliferyl‐β‐d‐glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme β‐glucuronidase, specificto Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media.
Conclusions: The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters.
Significance and Impact of the Study: The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time. |
| doi_str_mv | 10.1046/j.1365-2672.2002.01729.x |
| format | Article |
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Methods and Results: The fluorogenic substrate 4‐methylumbelliferyl‐β‐d‐glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme β‐glucuronidase, specificto Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media.
Conclusions: The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters.
Significance and Impact of the Study: The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2002.01729.x</identifier><identifier>PMID: 12234337</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>4-methylumbelliferyl- beta -D-glucuronide ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Colony Count, Microbial ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - isolation & purification ; Fluorescent Dyes - metabolism ; Fundamental and applied biological sciences. Psychology ; Glucuronidase - metabolism ; Hymecromone - analogs & derivatives ; Hymecromone - metabolism ; methylumbelliferone ; Microbiology ; Seawater - microbiology ; Time Factors ; Water Pollution</subject><ispartof>Journal of applied microbiology, 2002-01, Vol.93 (4), p.548-556</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4539-f1091dc007f863ea4e0e6da9de7f6a0d0a685fc234393e2339431e796b0144a83</citedby><cites>FETCH-LOGICAL-c4539-f1091dc007f863ea4e0e6da9de7f6a0d0a685fc234393e2339431e796b0144a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2002.01729.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2002.01729.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13915550$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12234337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caruso, G.</creatorcontrib><creatorcontrib>Crisafi, E.</creatorcontrib><creatorcontrib>Mancuso, M.</creatorcontrib><title>Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters.
Methods and Results: The fluorogenic substrate 4‐methylumbelliferyl‐β‐d‐glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme β‐glucuronidase, specificto Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media.
Conclusions: The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters.
Significance and Impact of the Study: The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time.</description><subject>4-methylumbelliferyl- beta -D-glucuronide</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - isolation & purification</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucuronidase - metabolism</subject><subject>Hymecromone - analogs & derivatives</subject><subject>Hymecromone - metabolism</subject><subject>methylumbelliferone</subject><subject>Microbiology</subject><subject>Seawater - microbiology</subject><subject>Time Factors</subject><subject>Water Pollution</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1v1DAQhi0EomXhLyALCW4JYztx4gOHqpQvFXEpZ2vqjFWv8rF4dmmXX0_SXajEidPMyM-MXj9CSAWlgsq-XZfK2LrQttGlBtAlqEa78u6ROP378Pi-r4oaGn0injGvAZSB2j4VJ0prUxnTnIqr9_ST-mkz0LiVU5Q4Shp_7QeSyIx7GacsM25St8zE_Ie74HBDOYWbhDJMfZJplEx4i1vK_Fw8idgzvTjWlfj-4eLq_FNx-e3j5_OzyyJUtXFFVOBUFwCa2FpDWBGQ7dB11ESL0AHato5hieoMaWNcZRQ1zl6DqipszUq8Odzd5OnHjnjrh8SB-h5HmnbslXOqmX88g6_-AdfTLo9zNq-Ndla3c6CVaA9QyBNzpug3OQ2Y916BX7T7tV_s-sWuX7T7e-3-bl59eby_ux6oe1g8ep6B10cAOWAfM44h8QNnnKrrGmbu3YG7TT3t_zuA_3L2denMb94FnIE</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Caruso, G.</creator><creator>Crisafi, E.</creator><creator>Mancuso, M.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope></search><sort><creationdate>20020101</creationdate><title>Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters</title><author>Caruso, G. ; Crisafi, E. ; Mancuso, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4539-f1091dc007f863ea4e0e6da9de7f6a0d0a685fc234393e2339431e796b0144a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>4-methylumbelliferyl- beta -D-glucuronide</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - isolation & purification</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucuronidase - metabolism</topic><topic>Hymecromone - analogs & derivatives</topic><topic>Hymecromone - metabolism</topic><topic>methylumbelliferone</topic><topic>Microbiology</topic><topic>Seawater - microbiology</topic><topic>Time Factors</topic><topic>Water Pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caruso, G.</creatorcontrib><creatorcontrib>Crisafi, E.</creatorcontrib><creatorcontrib>Mancuso, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caruso, G.</au><au>Crisafi, E.</au><au>Mancuso, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>93</volume><issue>4</issue><spage>548</spage><epage>556</epage><pages>548-556</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: An analytical protocol has been developed and applied for the detection of glucuronidase activity in marine waters as a rapid alternative approach to assess the microbiological quality of seawaters.
Methods and Results: The fluorogenic substrate 4‐methylumbelliferyl‐β‐d‐glucuronide is cleaved to a fluorescent product, methylumbelliferone, by the enzyme β‐glucuronidase, specificto Escherichia coli and closely related enterobacterial species (Shigella). The results suggest that this test is related to E. coli numbers, as estimated by immunofluorescence, more significantly than to faecal coliform numbers, obtained from culture media.
Conclusions: The determination of the potential rate of glucuronidase activity may be used as a diagnostic tool for the indirect estimation of the presence of E. coli in seawaters.
Significance and Impact of the Study: The method may be particularly useful in the early warning of seawater pollution, allowing the screening of coastal areas with different contamination levels in reduced time.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12234337</pmid><doi>10.1046/j.1365-2672.2002.01729.x</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 4-methylumbelliferyl- beta -D-glucuronide Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Colony Count, Microbial Escherichia coli Escherichia coli - enzymology Escherichia coli - isolation & purification Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Glucuronidase - metabolism Hymecromone - analogs & derivatives Hymecromone - metabolism methylumbelliferone Microbiology Seawater - microbiology Time Factors Water Pollution |
| title | Development of an enzyme assay for rapid assessment of Escherichia coli in seawaters |
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