DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer
Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid...
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| Veröffentlicht in: | Analyst (London) 2018-02, Vol.143 (4), p.914-919 |
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| creator | Wang, Yaoqi Gao, Jiaxue Meng, Xianying Wang, Zhenxin |
| description | Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid carcinoma (PTC) related mutation points including BRAF
(t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by the attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at the sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of the DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained. |
| doi_str_mv | 10.1039/c7an01773a |
| format | Article |
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(t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by the attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at the sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of the DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c7an01773a</identifier><identifier>PMID: 29362729</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Assaying ; Deoxyribonucleic acid ; DNA ; DNA Mutational Analysis - methods ; DNA, Single-Stranded ; Gold ; GTP Phosphohydrolases - genetics ; Humans ; Light scattering ; Membrane Proteins - genetics ; Metal Nanoparticles ; Multiplexing ; Mutation ; Oligonucleotide Array Sequence Analysis ; Polymorphism ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic ; Proto-Oncogene Proteins B-raf - genetics ; Resonance scattering ; Telomerase - genetics ; Thyroid cancer ; Thyroid Neoplasms - diagnosis ; Thyroid Neoplasms - genetics</subject><ispartof>Analyst (London), 2018-02, Vol.143 (4), p.914-919</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-27e2841601fdc9130bf455055d7a74b483e9da6628114109440b678fcb08e1603</citedby><cites>FETCH-LOGICAL-c315t-27e2841601fdc9130bf455055d7a74b483e9da6628114109440b678fcb08e1603</cites><orcidid>0000-0002-1908-9848</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2817,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29362729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yaoqi</creatorcontrib><creatorcontrib>Gao, Jiaxue</creatorcontrib><creatorcontrib>Meng, Xianying</creatorcontrib><creatorcontrib>Wang, Zhenxin</creatorcontrib><title>DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid carcinoma (PTC) related mutation points including BRAF
(t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by the attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at the sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of the DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained.</description><subject>Assaying</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Mutational Analysis - methods</subject><subject>DNA, Single-Stranded</subject><subject>Gold</subject><subject>GTP Phosphohydrolases - genetics</subject><subject>Humans</subject><subject>Light scattering</subject><subject>Membrane Proteins - genetics</subject><subject>Metal Nanoparticles</subject><subject>Multiplexing</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Promoter Regions, Genetic</subject><subject>Proto-Oncogene Proteins B-raf - genetics</subject><subject>Resonance scattering</subject><subject>Telomerase - genetics</subject><subject>Thyroid cancer</subject><subject>Thyroid Neoplasms - diagnosis</subject><subject>Thyroid Neoplasms - genetics</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1r3DAQhkVpaLZpL_0BRdBLKDgdfUvHZZs2hZBe2rORZTlRsC1XkqFL_ny1mzSHnIaBZx5m5kXoA4ELAsx8ccrOQJRi9hXaECZ5IwTVr9EGAFhDpeCn6G3O97UlIOANOqWGSaqo2aCHrzdbPAWXok3J7pvOZt_j5HOc7ew8HsPtXcHZ2VJ8CvMttjnbPR5iwtM6lrCM_m8d6H3xroQ44zjgo3It9tiHGS92CeNo0x6Xu32Kocfu4E7v0Mlgx-zfP9Uz9Pvb5a_dVXP98_uP3fa6cYyI0lDlqeZEAhl6ZwiDbuBCgBC9sop3XDNveisl1YRwAoZz6KTSg-tA-zrGztD5o3dJ8c_qc2mnkJ2vK80-rrklxoAWXEtW0U8v0Pu4prlu11IAI4nQ_CD8_EjVt-Wc_NAuKUz1wJZAe4ik3antzTGSbYU_PinXbvL9M_o_A_YPRq6GKA</recordid><startdate>20180221</startdate><enddate>20180221</enddate><creator>Wang, Yaoqi</creator><creator>Gao, Jiaxue</creator><creator>Meng, Xianying</creator><creator>Wang, Zhenxin</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1908-9848</orcidid></search><sort><creationdate>20180221</creationdate><title>DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer</title><author>Wang, Yaoqi ; Gao, Jiaxue ; Meng, Xianying ; Wang, Zhenxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-27e2841601fdc9130bf455055d7a74b483e9da6628114109440b678fcb08e1603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Assaying</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Mutational Analysis - methods</topic><topic>DNA, Single-Stranded</topic><topic>Gold</topic><topic>GTP Phosphohydrolases - genetics</topic><topic>Humans</topic><topic>Light scattering</topic><topic>Membrane Proteins - genetics</topic><topic>Metal Nanoparticles</topic><topic>Multiplexing</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Promoter Regions, Genetic</topic><topic>Proto-Oncogene Proteins B-raf - genetics</topic><topic>Resonance scattering</topic><topic>Telomerase - genetics</topic><topic>Thyroid cancer</topic><topic>Thyroid Neoplasms - diagnosis</topic><topic>Thyroid Neoplasms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yaoqi</creatorcontrib><creatorcontrib>Gao, Jiaxue</creatorcontrib><creatorcontrib>Meng, Xianying</creatorcontrib><creatorcontrib>Wang, Zhenxin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yaoqi</au><au>Gao, Jiaxue</au><au>Meng, Xianying</au><au>Wang, Zhenxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2018-02-21</date><risdate>2018</risdate><volume>143</volume><issue>4</issue><spage>914</spage><epage>919</epage><pages>914-919</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Highly accurate analysis of single-nucleotide polymorphisms (SNPs) plays an important role in both disease diagnostics and personalized medicine development. In this work, a DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of papillary thyroid carcinoma (PTC) related mutation points including BRAF
(t1m), NRAS codon 61 (t2m), TERT promoter g.1295228 (t31m) and TERT promoter g.1295250 (t32m) with high sensitivity and selectivity by the attachment of polyvalent ssDNA modified 13 nm gold nanoparticles (ssDNAs@GNPs) followed by silver deposition for signal enhancement. The microarray-based RLS assay provides a detection limit (S/N = 3) at the sub-nanomolar level for the target ssDNAs and determines allele frequencies as low as 0.2% for t1m, 0.2% for t2m, 0.5% for t31m, and 0.5% for t32m in the cocktail of target ssDNAs, respectively. The practicability of the DNA microarray-based RLS assay is demonstrated by profiling of t2m in 50 clinical thyroid tissue samples of PTC patients, and satisfactory results are obtained.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29362729</pmid><doi>10.1039/c7an01773a</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1908-9848</orcidid></addata></record> |
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| source | Royal Society of Chemistry Journals Archive (1841-2007); MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Assaying Deoxyribonucleic acid DNA DNA Mutational Analysis - methods DNA, Single-Stranded Gold GTP Phosphohydrolases - genetics Humans Light scattering Membrane Proteins - genetics Metal Nanoparticles Multiplexing Mutation Oligonucleotide Array Sequence Analysis Polymorphism Polymorphism, Single Nucleotide Promoter Regions, Genetic Proto-Oncogene Proteins B-raf - genetics Resonance scattering Telomerase - genetics Thyroid cancer Thyroid Neoplasms - diagnosis Thyroid Neoplasms - genetics |
| title | DNA microarray-based resonance light scattering assay for multiplexed detection of DNA mutation in papillary thyroid cancer |
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