Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients
Purpose To visualize by in situ hybridization (ISH) the levels of a set of proliferation-associated miRNAs and to evaluate their impact and clinical applicability in prognostication of invasive breast carcinoma. Methods Tissue specimen from breast carcinoma patients were investigated for miRNAs-494,...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2018-04, Vol.144 (4), p.657-666 |
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| creator | Gurvits, Natalia Autere, Tuomo-Artturi Repo, Heli Nykänen, Marjukka Kuopio, Teijo Kronqvist, Pauliina Talvinen, Kati |
| description | Purpose
To visualize by in situ hybridization (ISH) the levels of a set of proliferation-associated miRNAs and to evaluate their impact and clinical applicability in prognostication of invasive breast carcinoma.
Methods
Tissue specimen from breast carcinoma patients were investigated for miRNAs-494, -205, -21 and -126. Prognostic associations for levels of miRNAs were analyzed based on complete clinical data and up to 22.5-year follow-up of the patient material (
n
= 285). For detection of the miRNAs, an automated sensitive protocol applying in situ hybridization was developed.
Results
MiRNA-494 indicated prognostic value for patients with invasive breast carcinoma. Among node-negative disease reduced level of miRNA-494 predicted 8.5-fold risk of breast cancer death (
p
= 0.04). Altered levels and expression patterns of the studied miRNAs were observed in breast carcinomas as compared to benign breast tissue.
Conclusions
The present paper reports for the first time on the prognostic value of miRNA-494 in invasive breast cancer. Particularly, detection of miRNA-494 could benefit patients with node-negative breast cancer in identifying subgroups with aggressive disease. Based on our experience, the developed automatic ISH method to visualize altered levels of miRNAs-494, -205, -21 and -126 could be applied to routine pathology diagnostics providing that conditions of tissue treatment, especially fixation delays, are managed. |
| doi_str_mv | 10.1007/s00432-018-2586-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1990854237</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1990854237</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-becbdcdbe38bab354ed4f8262a93f68d124ba3091a9f14baa5a60db9855e96723</originalsourceid><addsrcrecordid>eNp1kcuKFTEQhoMoznH0AdxIwI0Lo7l0ujvuhsEbDCqi6yaX6jFDd-eYSsMcX8WXNT1nFBHcJFWp7_9T8BPyWPAXgvPuJXLeKMm46JnUfcv6O2QnthehlL5Ldlx0gmkp2hPyAPGK11538j45kUa10gizIz8_5TTFEbItMS3MIiYfbYFA5_j5wxmyxjTPKZNcb6egdgmUCdnSAAX8xrkDjQvFWFb67eByDPHHjdcrCtf7DIi1vpHtc7pcEpbo6T4VWEq00yZ1GSwW6m32cUmzpfuqr2N8SO6NdkJ4dHufkq9vXn85f8cuPr59f352wbzqZGEOvAs-OFC9s07pBkIz9rKV1qix7YOQjbOKG2HNKGpptW15cKbXGkzbSXVKnh1964bfV8AyzBE9TJNdIK04CGN4rxupuoo-_Qe9Smte6nYbpVTTKdNWShwpnxNihnHY5zjbfBgEH7bkhmNyQ01u2JIb-qp5cuu8uhnCH8XvqCogjwDW0XIJ-a-v_-v6CzJMo-o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993347396</pqid></control><display><type>article</type><title>Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Gurvits, Natalia ; Autere, Tuomo-Artturi ; Repo, Heli ; Nykänen, Marjukka ; Kuopio, Teijo ; Kronqvist, Pauliina ; Talvinen, Kati</creator><creatorcontrib>Gurvits, Natalia ; Autere, Tuomo-Artturi ; Repo, Heli ; Nykänen, Marjukka ; Kuopio, Teijo ; Kronqvist, Pauliina ; Talvinen, Kati</creatorcontrib><description>Purpose
To visualize by in situ hybridization (ISH) the levels of a set of proliferation-associated miRNAs and to evaluate their impact and clinical applicability in prognostication of invasive breast carcinoma.
Methods
Tissue specimen from breast carcinoma patients were investigated for miRNAs-494, -205, -21 and -126. Prognostic associations for levels of miRNAs were analyzed based on complete clinical data and up to 22.5-year follow-up of the patient material (
n
= 285). For detection of the miRNAs, an automated sensitive protocol applying in situ hybridization was developed.
Results
MiRNA-494 indicated prognostic value for patients with invasive breast carcinoma. Among node-negative disease reduced level of miRNA-494 predicted 8.5-fold risk of breast cancer death (
p
= 0.04). Altered levels and expression patterns of the studied miRNAs were observed in breast carcinomas as compared to benign breast tissue.
Conclusions
The present paper reports for the first time on the prognostic value of miRNA-494 in invasive breast cancer. Particularly, detection of miRNA-494 could benefit patients with node-negative breast cancer in identifying subgroups with aggressive disease. Based on our experience, the developed automatic ISH method to visualize altered levels of miRNAs-494, -205, -21 and -126 could be applied to routine pathology diagnostics providing that conditions of tissue treatment, especially fixation delays, are managed.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-018-2586-8</identifier><identifier>PMID: 29362919</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Breast cancer ; Breast carcinoma ; Breast Neoplasms - genetics ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Breasts ; Cancer Research ; Cell Proliferation - genetics ; Female ; Gene expression ; Hematology ; Humans ; Hybridization ; In Situ Hybridization ; Internal Medicine ; Invasiveness ; Mammography ; Medical prognosis ; Medicine ; Medicine & Public Health ; MicroRNAs - biosynthesis ; MicroRNAs - genetics ; Middle Aged ; miRNA ; Oncology ; Original Article – Cancer Research ; Prognosis</subject><ispartof>Journal of cancer research and clinical oncology, 2018-04, Vol.144 (4), p.657-666</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Journal of Cancer Research and Clinical Oncology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-becbdcdbe38bab354ed4f8262a93f68d124ba3091a9f14baa5a60db9855e96723</citedby><cites>FETCH-LOGICAL-c372t-becbdcdbe38bab354ed4f8262a93f68d124ba3091a9f14baa5a60db9855e96723</cites><orcidid>0000-0003-0550-3064</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00432-018-2586-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00432-018-2586-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29362919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gurvits, Natalia</creatorcontrib><creatorcontrib>Autere, Tuomo-Artturi</creatorcontrib><creatorcontrib>Repo, Heli</creatorcontrib><creatorcontrib>Nykänen, Marjukka</creatorcontrib><creatorcontrib>Kuopio, Teijo</creatorcontrib><creatorcontrib>Kronqvist, Pauliina</creatorcontrib><creatorcontrib>Talvinen, Kati</creatorcontrib><title>Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Purpose
To visualize by in situ hybridization (ISH) the levels of a set of proliferation-associated miRNAs and to evaluate their impact and clinical applicability in prognostication of invasive breast carcinoma.
Methods
Tissue specimen from breast carcinoma patients were investigated for miRNAs-494, -205, -21 and -126. Prognostic associations for levels of miRNAs were analyzed based on complete clinical data and up to 22.5-year follow-up of the patient material (
n
= 285). For detection of the miRNAs, an automated sensitive protocol applying in situ hybridization was developed.
Results
MiRNA-494 indicated prognostic value for patients with invasive breast carcinoma. Among node-negative disease reduced level of miRNA-494 predicted 8.5-fold risk of breast cancer death (
p
= 0.04). Altered levels and expression patterns of the studied miRNAs were observed in breast carcinomas as compared to benign breast tissue.
Conclusions
The present paper reports for the first time on the prognostic value of miRNA-494 in invasive breast cancer. Particularly, detection of miRNA-494 could benefit patients with node-negative breast cancer in identifying subgroups with aggressive disease. Based on our experience, the developed automatic ISH method to visualize altered levels of miRNAs-494, -205, -21 and -126 could be applied to routine pathology diagnostics providing that conditions of tissue treatment, especially fixation delays, are managed.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Breast cancer</subject><subject>Breast carcinoma</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breasts</subject><subject>Cancer Research</subject><subject>Cell Proliferation - genetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Hematology</subject><subject>Humans</subject><subject>Hybridization</subject><subject>In Situ Hybridization</subject><subject>Internal Medicine</subject><subject>Invasiveness</subject><subject>Mammography</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs - biosynthesis</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Oncology</subject><subject>Original Article – Cancer Research</subject><subject>Prognosis</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kcuKFTEQhoMoznH0AdxIwI0Lo7l0ujvuhsEbDCqi6yaX6jFDd-eYSsMcX8WXNT1nFBHcJFWp7_9T8BPyWPAXgvPuJXLeKMm46JnUfcv6O2QnthehlL5Ldlx0gmkp2hPyAPGK11538j45kUa10gizIz8_5TTFEbItMS3MIiYfbYFA5_j5wxmyxjTPKZNcb6egdgmUCdnSAAX8xrkDjQvFWFb67eByDPHHjdcrCtf7DIi1vpHtc7pcEpbo6T4VWEq00yZ1GSwW6m32cUmzpfuqr2N8SO6NdkJ4dHufkq9vXn85f8cuPr59f352wbzqZGEOvAs-OFC9s07pBkIz9rKV1qix7YOQjbOKG2HNKGpptW15cKbXGkzbSXVKnh1964bfV8AyzBE9TJNdIK04CGN4rxupuoo-_Qe9Smte6nYbpVTTKdNWShwpnxNihnHY5zjbfBgEH7bkhmNyQ01u2JIb-qp5cuu8uhnCH8XvqCogjwDW0XIJ-a-v_-v6CzJMo-o</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Gurvits, Natalia</creator><creator>Autere, Tuomo-Artturi</creator><creator>Repo, Heli</creator><creator>Nykänen, Marjukka</creator><creator>Kuopio, Teijo</creator><creator>Kronqvist, Pauliina</creator><creator>Talvinen, Kati</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0550-3064</orcidid></search><sort><creationdate>20180401</creationdate><title>Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients</title><author>Gurvits, Natalia ; Autere, Tuomo-Artturi ; Repo, Heli ; Nykänen, Marjukka ; Kuopio, Teijo ; Kronqvist, Pauliina ; Talvinen, Kati</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-becbdcdbe38bab354ed4f8262a93f68d124ba3091a9f14baa5a60db9855e96723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Breast cancer</topic><topic>Breast carcinoma</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breasts</topic><topic>Cancer Research</topic><topic>Cell Proliferation - genetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Hematology</topic><topic>Humans</topic><topic>Hybridization</topic><topic>In Situ Hybridization</topic><topic>Internal Medicine</topic><topic>Invasiveness</topic><topic>Mammography</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNAs - biosynthesis</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>Oncology</topic><topic>Original Article – Cancer Research</topic><topic>Prognosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gurvits, Natalia</creatorcontrib><creatorcontrib>Autere, Tuomo-Artturi</creatorcontrib><creatorcontrib>Repo, Heli</creatorcontrib><creatorcontrib>Nykänen, Marjukka</creatorcontrib><creatorcontrib>Kuopio, Teijo</creatorcontrib><creatorcontrib>Kronqvist, Pauliina</creatorcontrib><creatorcontrib>Talvinen, Kati</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gurvits, Natalia</au><au>Autere, Tuomo-Artturi</au><au>Repo, Heli</au><au>Nykänen, Marjukka</au><au>Kuopio, Teijo</au><au>Kronqvist, Pauliina</au><au>Talvinen, Kati</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>144</volume><issue>4</issue><spage>657</spage><epage>666</epage><pages>657-666</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><abstract>Purpose
To visualize by in situ hybridization (ISH) the levels of a set of proliferation-associated miRNAs and to evaluate their impact and clinical applicability in prognostication of invasive breast carcinoma.
Methods
Tissue specimen from breast carcinoma patients were investigated for miRNAs-494, -205, -21 and -126. Prognostic associations for levels of miRNAs were analyzed based on complete clinical data and up to 22.5-year follow-up of the patient material (
n
= 285). For detection of the miRNAs, an automated sensitive protocol applying in situ hybridization was developed.
Results
MiRNA-494 indicated prognostic value for patients with invasive breast carcinoma. Among node-negative disease reduced level of miRNA-494 predicted 8.5-fold risk of breast cancer death (
p
= 0.04). Altered levels and expression patterns of the studied miRNAs were observed in breast carcinomas as compared to benign breast tissue.
Conclusions
The present paper reports for the first time on the prognostic value of miRNA-494 in invasive breast cancer. Particularly, detection of miRNA-494 could benefit patients with node-negative breast cancer in identifying subgroups with aggressive disease. Based on our experience, the developed automatic ISH method to visualize altered levels of miRNAs-494, -205, -21 and -126 could be applied to routine pathology diagnostics providing that conditions of tissue treatment, especially fixation delays, are managed.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29362919</pmid><doi>10.1007/s00432-018-2586-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0550-3064</orcidid></addata></record> |
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| identifier | ISSN: 0171-5216 |
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| issn | 0171-5216 1432-1335 |
| language | eng |
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| subjects | Adult Aged Aged, 80 and over Breast cancer Breast carcinoma Breast Neoplasms - genetics Breast Neoplasms - metabolism Breast Neoplasms - pathology Breasts Cancer Research Cell Proliferation - genetics Female Gene expression Hematology Humans Hybridization In Situ Hybridization Internal Medicine Invasiveness Mammography Medical prognosis Medicine Medicine & Public Health MicroRNAs - biosynthesis MicroRNAs - genetics Middle Aged miRNA Oncology Original Article – Cancer Research Prognosis |
| title | Proliferation-associated miRNAs-494, -205, -21 and -126 detected by in situ hybridization: expression and prognostic potential in breast carcinoma patients |
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