Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells
Cripto-1 (CR-1) is a member of the epidermal growth factor-Cripto-1/FRL1/Cryptic gene family that plays a key role in the various malignant cancers. However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1 was down-regulated by small interfering RNA (siRNA). West...
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| description | Cripto-1 (CR-1) is a member of the epidermal growth factor-Cripto-1/FRL1/Cryptic gene family that plays a key role in the various malignant cancers. However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1 was down-regulated by small interfering RNA (siRNA). Western blot measured the expression levels of CR-1 and some related proteins. We performed Cell Counting Kit-8, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and flow cytometry to detect the cellular proliferation and cycle. The transwell assay was used to observe cellular migration and invasion. The ability of angiogenesis was evaluated by tube formation assay. Our results showed that CR-1 knockdown markedly inhibited cell proliferation and induced cycle arrest in G1 phase, as p21 and p27 were up-regulated, whereas cyclin D1 and cyclin E1 were diminished. Moreover, silencing of CR-1 dramatically inhibited cell migration and invasion, repressed matrix metalloproteinases, and disturbed epithelial-mesenchymal transition. CR-1 siRNA suppressed the secreted level of vascular endothelial growth factor, and reduced protein level of Vascular endothelial growth factor receptor 2. We further found that decreased CR-1 expression inhibited FAK/Src/PI3K and Wnt/β-catenin signalling in PCa cells. These results suggested CR-1 might be served as an effective therapeutic target in PCa. |
| doi_str_mv | 10.1007/s12038-017-9700-y |
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However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1 was down-regulated by small interfering RNA (siRNA). Western blot measured the expression levels of CR-1 and some related proteins. We performed Cell Counting Kit-8, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and flow cytometry to detect the cellular proliferation and cycle. The transwell assay was used to observe cellular migration and invasion. The ability of angiogenesis was evaluated by tube formation assay. Our results showed that CR-1 knockdown markedly inhibited cell proliferation and induced cycle arrest in G1 phase, as p21 and p27 were up-regulated, whereas cyclin D1 and cyclin E1 were diminished. Moreover, silencing of CR-1 dramatically inhibited cell migration and invasion, repressed matrix metalloproteinases, and disturbed epithelial-mesenchymal transition. CR-1 siRNA suppressed the secreted level of vascular endothelial growth factor, and reduced protein level of Vascular endothelial growth factor receptor 2. We further found that decreased CR-1 expression inhibited FAK/Src/PI3K and Wnt/β-catenin signalling in PCa cells. These results suggested CR-1 might be served as an effective therapeutic target in PCa.</description><identifier>ISSN: 0250-5991</identifier><identifier>EISSN: 0973-7138</identifier><identifier>DOI: 10.1007/s12038-017-9700-y</identifier><identifier>PMID: 29358554</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>1-Phosphatidylinositol 3-kinase ; Angiogenesis ; Apoptosis - genetics ; Assaying ; beta Catenin - genetics ; beta Catenin - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Cell adhesion & migration ; Cell Biology ; Cell Line, Tumor ; Cell migration ; Cell Movement ; Cell Proliferation ; Chromium ; Cryptic gene ; Cyclin D1 ; Cyclin D1 - genetics ; Cyclin D1 - metabolism ; Cyclin E - genetics ; Cyclin E - metabolism ; Cyclin-Dependent Kinase Inhibitor p21 - genetics ; Cyclin-Dependent Kinase Inhibitor p21 - metabolism ; Cyclin-Dependent Kinase Inhibitor p27 - genetics ; Cyclin-Dependent Kinase Inhibitor p27 - metabolism ; Cytometry ; Epidermal growth factor ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Epithelial-Mesenchymal Transition - genetics ; Flow cytometry ; Focal Adhesion Kinase 1 - genetics ; Focal Adhesion Kinase 1 - metabolism ; G1 phase ; G1 Phase Cell Cycle Checkpoints - genetics ; Gene expression ; Gene Expression Regulation, Neoplastic ; GPI-Linked Proteins - antagonists & inhibitors ; GPI-Linked Proteins - genetics ; GPI-Linked Proteins - metabolism ; Growth factors ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Life Sciences ; Male ; Matrix metalloproteinases ; Mesenchyme ; Microbiology ; Neoplasm Proteins - antagonists & inhibitors ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Oncogene Proteins - genetics ; Oncogene Proteins - metabolism ; Phosphatidylinositol 3-Kinases - genetics ; Phosphatidylinositol 3-Kinases - metabolism ; Plant Sciences ; Prostate ; Prostate - metabolism ; Prostate - pathology ; Prostate cancer ; Prostate carcinoma ; Proteins ; Ribonucleic acid ; RNA ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Signaling ; siRNA ; src-Family Kinases - genetics ; src-Family Kinases - metabolism ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Vascular endothelial growth factor receptor 2 ; Vascular Endothelial Growth Factor Receptor-2 - genetics ; Vascular Endothelial Growth Factor Receptor-2 - metabolism ; Wnt protein ; Wnt Proteins - genetics ; Wnt Proteins - metabolism ; Wnt Signaling Pathway ; Zoology ; β-Catenin</subject><ispartof>Journal of biosciences, 2017-09, Vol.42 (3), p.405-416</ispartof><rights>Indian Academy of Sciences 2017</rights><rights>Journal of Biosciences is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-22e38961a7ddcebb0a5f9701ade3db4be90926f796276281b9c7654f831c8ad93</citedby><cites>FETCH-LOGICAL-c372t-22e38961a7ddcebb0a5f9701ade3db4be90926f796276281b9c7654f831c8ad93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12038-017-9700-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12038-017-9700-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29358554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Ding</creatorcontrib><creatorcontrib>Shi, Zhan</creatorcontrib><creatorcontrib>Xu, Hao</creatorcontrib><creatorcontrib>Chen, Renfu</creatorcontrib><creatorcontrib>Xue, Song</creatorcontrib><creatorcontrib>Sun, Xiaoqing</creatorcontrib><title>Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells</title><title>Journal of biosciences</title><addtitle>J Biosci</addtitle><addtitle>J Biosci</addtitle><description>Cripto-1 (CR-1) is a member of the epidermal growth factor-Cripto-1/FRL1/Cryptic gene family that plays a key role in the various malignant cancers. However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1 was down-regulated by small interfering RNA (siRNA). Western blot measured the expression levels of CR-1 and some related proteins. We performed Cell Counting Kit-8, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and flow cytometry to detect the cellular proliferation and cycle. The transwell assay was used to observe cellular migration and invasion. The ability of angiogenesis was evaluated by tube formation assay. Our results showed that CR-1 knockdown markedly inhibited cell proliferation and induced cycle arrest in G1 phase, as p21 and p27 were up-regulated, whereas cyclin D1 and cyclin E1 were diminished. Moreover, silencing of CR-1 dramatically inhibited cell migration and invasion, repressed matrix metalloproteinases, and disturbed epithelial-mesenchymal transition. CR-1 siRNA suppressed the secreted level of vascular endothelial growth factor, and reduced protein level of Vascular endothelial growth factor receptor 2. We further found that decreased CR-1 expression inhibited FAK/Src/PI3K and Wnt/β-catenin signalling in PCa cells. These results suggested CR-1 might be served as an effective therapeutic target in PCa.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>Angiogenesis</subject><subject>Apoptosis - genetics</subject><subject>Assaying</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell adhesion & migration</subject><subject>Cell Biology</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Chromium</subject><subject>Cryptic gene</subject><subject>Cyclin D1</subject><subject>Cyclin D1 - genetics</subject><subject>Cyclin D1 - metabolism</subject><subject>Cyclin E - genetics</subject><subject>Cyclin E - metabolism</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</subject><subject>Cyclin-Dependent Kinase Inhibitor p27 - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor p27 - metabolism</subject><subject>Cytometry</subject><subject>Epidermal growth factor</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Flow cytometry</subject><subject>Focal Adhesion Kinase 1 - genetics</subject><subject>Focal Adhesion Kinase 1 - metabolism</subject><subject>G1 phase</subject><subject>G1 Phase Cell Cycle Checkpoints - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>GPI-Linked Proteins - antagonists & inhibitors</subject><subject>GPI-Linked Proteins - genetics</subject><subject>GPI-Linked Proteins - metabolism</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Matrix metalloproteinases</subject><subject>Mesenchyme</subject><subject>Microbiology</subject><subject>Neoplasm Proteins - antagonists & inhibitors</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Oncogene Proteins - genetics</subject><subject>Oncogene Proteins - metabolism</subject><subject>Phosphatidylinositol 3-Kinases - genetics</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Plant Sciences</subject><subject>Prostate</subject><subject>Prostate - metabolism</subject><subject>Prostate - pathology</subject><subject>Prostate cancer</subject><subject>Prostate carcinoma</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Signaling</subject><subject>siRNA</subject><subject>src-Family Kinases - genetics</subject><subject>src-Family Kinases - metabolism</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vascular endothelial growth factor receptor 2</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - genetics</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - metabolism</subject><subject>Wnt protein</subject><subject>Wnt Proteins - genetics</subject><subject>Wnt Proteins - metabolism</subject><subject>Wnt Signaling Pathway</subject><subject>Zoology</subject><subject>β-Catenin</subject><issn>0250-5991</issn><issn>0973-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kUtLAzEUhYMotlZ_gBsZcOPCaB4zk2QpxRcW3Og6ZDKZNrWT1GRG6b83ta2I4CLkwv3OvedyADjF6AojxK4jJohyiDCDgiEEV3tgiASjkGHK91NNCgQLIfAAHMU4RwiLnKJDMCCCFrwo8iGIT87rt9p_usw32TjYZechzqyb2cp2MetmJlsGv7CNCaqz3l1mrZ3uSus-VPyulKvTm1o_Nc5EG1NrrYud6kymVdDW-VZl2iwW8RgcNGoRzcn2H4HXu9uX8QOcPN8_jm8mUFNGOkiIoVyUWLG61qaqkCqadCZWtaF1lVdGIEHKhomSsJJwXAnNyiJvOMWaq1rQEbjYzE1G3nsTO9nauHagnPF9lFgIlHMhWJ7Q8z_o3PfBJXeJohSznJU8UXhD6XRZDKaRy2BbFVYSI7lORG4SkSkRuU5ErpLmbDu5r1pT_yh2ESSAbICYWm5qwq_V_079At9Jl70</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Wu, Ding</creator><creator>Shi, Zhan</creator><creator>Xu, Hao</creator><creator>Chen, Renfu</creator><creator>Xue, Song</creator><creator>Sun, Xiaoqing</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20170901</creationdate><title>Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells</title><author>Wu, Ding ; Shi, Zhan ; Xu, Hao ; Chen, Renfu ; Xue, Song ; Sun, Xiaoqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-22e38961a7ddcebb0a5f9701ade3db4be90926f796276281b9c7654f831c8ad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>Angiogenesis</topic><topic>Apoptosis - genetics</topic><topic>Assaying</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell adhesion & migration</topic><topic>Cell Biology</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Chromium</topic><topic>Cryptic gene</topic><topic>Cyclin D1</topic><topic>Cyclin D1 - genetics</topic><topic>Cyclin D1 - metabolism</topic><topic>Cyclin E - genetics</topic><topic>Cyclin E - metabolism</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor p21 - metabolism</topic><topic>Cyclin-Dependent Kinase Inhibitor p27 - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor p27 - metabolism</topic><topic>Cytometry</topic><topic>Epidermal growth factor</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Flow cytometry</topic><topic>Focal Adhesion Kinase 1 - genetics</topic><topic>Focal Adhesion Kinase 1 - metabolism</topic><topic>G1 phase</topic><topic>G1 Phase Cell Cycle Checkpoints - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>GPI-Linked Proteins - antagonists & inhibitors</topic><topic>GPI-Linked Proteins - genetics</topic><topic>GPI-Linked Proteins - metabolism</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Matrix metalloproteinases</topic><topic>Mesenchyme</topic><topic>Microbiology</topic><topic>Neoplasm Proteins - antagonists & inhibitors</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Oncogene Proteins - genetics</topic><topic>Oncogene Proteins - metabolism</topic><topic>Phosphatidylinositol 3-Kinases - genetics</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Plant Sciences</topic><topic>Prostate</topic><topic>Prostate - metabolism</topic><topic>Prostate - pathology</topic><topic>Prostate cancer</topic><topic>Prostate carcinoma</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Signaling</topic><topic>siRNA</topic><topic>src-Family Kinases - genetics</topic><topic>src-Family Kinases - metabolism</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vascular endothelial growth factor receptor 2</topic><topic>Vascular Endothelial Growth Factor Receptor-2 - genetics</topic><topic>Vascular Endothelial Growth Factor Receptor-2 - metabolism</topic><topic>Wnt protein</topic><topic>Wnt Proteins - 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Academic</collection><jtitle>Journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Ding</au><au>Shi, Zhan</au><au>Xu, Hao</au><au>Chen, Renfu</au><au>Xue, Song</au><au>Sun, Xiaoqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells</atitle><jtitle>Journal of biosciences</jtitle><stitle>J Biosci</stitle><addtitle>J Biosci</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>42</volume><issue>3</issue><spage>405</spage><epage>416</epage><pages>405-416</pages><issn>0250-5991</issn><eissn>0973-7138</eissn><abstract>Cripto-1 (CR-1) is a member of the epidermal growth factor-Cripto-1/FRL1/Cryptic gene family that plays a key role in the various malignant cancers. However, the role of CR-1 in prostate carcinoma (PCa) remains limited. The expression of CR-1 was down-regulated by small interfering RNA (siRNA). Western blot measured the expression levels of CR-1 and some related proteins. We performed Cell Counting Kit-8, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and flow cytometry to detect the cellular proliferation and cycle. The transwell assay was used to observe cellular migration and invasion. The ability of angiogenesis was evaluated by tube formation assay. Our results showed that CR-1 knockdown markedly inhibited cell proliferation and induced cycle arrest in G1 phase, as p21 and p27 were up-regulated, whereas cyclin D1 and cyclin E1 were diminished. Moreover, silencing of CR-1 dramatically inhibited cell migration and invasion, repressed matrix metalloproteinases, and disturbed epithelial-mesenchymal transition. CR-1 siRNA suppressed the secreted level of vascular endothelial growth factor, and reduced protein level of Vascular endothelial growth factor receptor 2. We further found that decreased CR-1 expression inhibited FAK/Src/PI3K and Wnt/β-catenin signalling in PCa cells. These results suggested CR-1 might be served as an effective therapeutic target in PCa.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>29358554</pmid><doi>10.1007/s12038-017-9700-y</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0250-5991 |
| ispartof | Journal of biosciences, 2017-09, Vol.42 (3), p.405-416 |
| issn | 0250-5991 0973-7138 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1990489974 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; Indian Academy of Sciences |
| subjects | 1-Phosphatidylinositol 3-kinase Angiogenesis Apoptosis - genetics Assaying beta Catenin - genetics beta Catenin - metabolism Biomedical and Life Sciences Biomedicine Cell adhesion & migration Cell Biology Cell Line, Tumor Cell migration Cell Movement Cell Proliferation Chromium Cryptic gene Cyclin D1 Cyclin D1 - genetics Cyclin D1 - metabolism Cyclin E - genetics Cyclin E - metabolism Cyclin-Dependent Kinase Inhibitor p21 - genetics Cyclin-Dependent Kinase Inhibitor p21 - metabolism Cyclin-Dependent Kinase Inhibitor p27 - genetics Cyclin-Dependent Kinase Inhibitor p27 - metabolism Cytometry Epidermal growth factor Epithelial Cells - metabolism Epithelial Cells - pathology Epithelial-Mesenchymal Transition - genetics Flow cytometry Focal Adhesion Kinase 1 - genetics Focal Adhesion Kinase 1 - metabolism G1 phase G1 Phase Cell Cycle Checkpoints - genetics Gene expression Gene Expression Regulation, Neoplastic GPI-Linked Proteins - antagonists & inhibitors GPI-Linked Proteins - genetics GPI-Linked Proteins - metabolism Growth factors Humans Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Life Sciences Male Matrix metalloproteinases Mesenchyme Microbiology Neoplasm Proteins - antagonists & inhibitors Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Oncogene Proteins - genetics Oncogene Proteins - metabolism Phosphatidylinositol 3-Kinases - genetics Phosphatidylinositol 3-Kinases - metabolism Plant Sciences Prostate Prostate - metabolism Prostate - pathology Prostate cancer Prostate carcinoma Proteins Ribonucleic acid RNA RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Signaling siRNA src-Family Kinases - genetics src-Family Kinases - metabolism Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism Vascular endothelial growth factor receptor 2 Vascular Endothelial Growth Factor Receptor-2 - genetics Vascular Endothelial Growth Factor Receptor-2 - metabolism Wnt protein Wnt Proteins - genetics Wnt Proteins - metabolism Wnt Signaling Pathway Zoology β-Catenin |
| title | Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T17%3A48%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Knockdown%20of%20Cripto-1%20inhibits%20the%20proliferation,%20migration,%20invasion,%20and%20angiogenesis%20in%20prostate%20carcinoma%20cells&rft.jtitle=Journal%20of%20biosciences&rft.au=Wu,%20Ding&rft.date=2017-09-01&rft.volume=42&rft.issue=3&rft.spage=405&rft.epage=416&rft.pages=405-416&rft.issn=0250-5991&rft.eissn=0973-7138&rft_id=info:doi/10.1007/s12038-017-9700-y&rft_dat=%3Cproquest_cross%3E1933174768%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1933174768&rft_id=info:pmid/29358554&rfr_iscdi=true |