Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway
Asiatic acid, a triterpenoid derived from Centella asiatica, has been found to exhibit multiple bioactivities. In this study, we investigated the effects of asiatic acid on lipopolysaccharide (LPS)-induced neuroinflammation and explored the mechanism of its action in BV2 microglia. We found that asi...
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| Veröffentlicht in: | Food & function 2018-02, Vol.9 (2), p.1048-1057 |
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| creator | Qian, Yisong Xin, Zhaochen Lv, Yanni Wang, Ziwei Zuo, Li Huang, Xiang Li, Yunman Xin, Hong-Bo |
| description | Asiatic acid, a triterpenoid derived from Centella asiatica, has been found to exhibit multiple bioactivities. In this study, we investigated the effects of asiatic acid on lipopolysaccharide (LPS)-induced neuroinflammation and explored the mechanism of its action in BV2 microglia. We found that asiatic acid (0.1 to 100 μM) treatment significantly attenuated nitric oxide (NO) production and inhibited inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner following LPS exposure. Asiatic acid reduced LPS-induced expression and secretion of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in BV2 cells. In addition, asiatic acid enhanced Sirt1 expression, reduced NF-κB p65 acetylation, and suppressed NF-κB activation after LPS stimulation. However, EX-527, an inhibitor of Sirt1, abolished the inhibitory effects of asiatic acid on LPS-stimulated microglia activation. These findings suggest that asiatic acid prevents LPS-induced neuroinflammation via regulating the Sirt1/NF-κB signaling pathway. |
| doi_str_mv | 10.1039/c7fo01442b |
| format | Article |
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In this study, we investigated the effects of asiatic acid on lipopolysaccharide (LPS)-induced neuroinflammation and explored the mechanism of its action in BV2 microglia. We found that asiatic acid (0.1 to 100 μM) treatment significantly attenuated nitric oxide (NO) production and inhibited inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner following LPS exposure. Asiatic acid reduced LPS-induced expression and secretion of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in BV2 cells. In addition, asiatic acid enhanced Sirt1 expression, reduced NF-κB p65 acetylation, and suppressed NF-κB activation after LPS stimulation. However, EX-527, an inhibitor of Sirt1, abolished the inhibitory effects of asiatic acid on LPS-stimulated microglia activation. These findings suggest that asiatic acid prevents LPS-induced neuroinflammation via regulating the Sirt1/NF-κB signaling pathway.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c7fo01442b</identifier><identifier>PMID: 29354820</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Acetylation ; Acids ; Activation ; Cytokines ; Inflammation ; Interleukin 6 ; Lipopolysaccharides ; Microglia ; NF-κB protein ; Nitric oxide ; Nitric-oxide synthase ; Signal transduction ; Signaling ; SIRT1 protein ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α</subject><ispartof>Food & function, 2018-02, Vol.9 (2), p.1048-1057</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-79c803f82efee33edb9d958ca8388452536cb6e9a7859787b7c8f1cfffca17823</citedby><cites>FETCH-LOGICAL-c315t-79c803f82efee33edb9d958ca8388452536cb6e9a7859787b7c8f1cfffca17823</cites><orcidid>0000-0001-6251-6756</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29354820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qian, Yisong</creatorcontrib><creatorcontrib>Xin, Zhaochen</creatorcontrib><creatorcontrib>Lv, Yanni</creatorcontrib><creatorcontrib>Wang, Ziwei</creatorcontrib><creatorcontrib>Zuo, Li</creatorcontrib><creatorcontrib>Huang, Xiang</creatorcontrib><creatorcontrib>Li, Yunman</creatorcontrib><creatorcontrib>Xin, Hong-Bo</creatorcontrib><title>Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>Asiatic acid, a triterpenoid derived from Centella asiatica, has been found to exhibit multiple bioactivities. 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These findings suggest that asiatic acid prevents LPS-induced neuroinflammation via regulating the Sirt1/NF-κB signaling pathway.</description><subject>Acetylation</subject><subject>Acids</subject><subject>Activation</subject><subject>Cytokines</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Lipopolysaccharides</subject><subject>Microglia</subject><subject>NF-κB protein</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>SIRT1 protein</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkc1KxDAQx4MoKutefAAJeBGhbj7aJjnuLq4K4h78wFtJ02TN0jY1aRVfzYfwmYyuenBgmIH58WfmPwAcYnSGERUTxYxDOE1JuQX2CUpJkmfocfu3T0W-B8YhrFEMKgQXfBfsEUGzlBO0D9w0WNlbBaWyFQxD13kdgg6w1YN3tjW1bJoIuBbaFs4eCGys8m5VWwlfYjauGurN3BnYP2l4a32PJzeL5ON9BoNdtbK27Qp2sn96lW8HYMfIOujxTx2B-8X53fwyuV5eXM2n14miOOsTJhRH1HCijdaU6qoUlci4kpxynmYko7kqcy0k45lgnJVMcYOVMUZJzDihI3Cy0e28ex506IvGBqXrWrbaDaHA0QmBs-hIRI__oWs3-Lh2KAjCiOeEsDxSpxsqXh-C16bovG2kfyswKr4-UczZYvn9iVmEj34kh7LR1R_66zv9BMHFhD8</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Qian, Yisong</creator><creator>Xin, Zhaochen</creator><creator>Lv, Yanni</creator><creator>Wang, Ziwei</creator><creator>Zuo, Li</creator><creator>Huang, Xiang</creator><creator>Li, Yunman</creator><creator>Xin, Hong-Bo</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6251-6756</orcidid></search><sort><creationdate>20180201</creationdate><title>Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway</title><author>Qian, Yisong ; Xin, Zhaochen ; Lv, Yanni ; Wang, Ziwei ; Zuo, Li ; Huang, Xiang ; Li, Yunman ; Xin, Hong-Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-79c803f82efee33edb9d958ca8388452536cb6e9a7859787b7c8f1cfffca17823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Acetylation</topic><topic>Acids</topic><topic>Activation</topic><topic>Cytokines</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Lipopolysaccharides</topic><topic>Microglia</topic><topic>NF-κB protein</topic><topic>Nitric oxide</topic><topic>Nitric-oxide synthase</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>SIRT1 protein</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qian, Yisong</creatorcontrib><creatorcontrib>Xin, Zhaochen</creatorcontrib><creatorcontrib>Lv, Yanni</creatorcontrib><creatorcontrib>Wang, Ziwei</creatorcontrib><creatorcontrib>Zuo, Li</creatorcontrib><creatorcontrib>Huang, Xiang</creatorcontrib><creatorcontrib>Li, Yunman</creatorcontrib><creatorcontrib>Xin, Hong-Bo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qian, Yisong</au><au>Xin, Zhaochen</au><au>Lv, Yanni</au><au>Wang, Ziwei</au><au>Zuo, Li</au><au>Huang, Xiang</au><au>Li, Yunman</au><au>Xin, Hong-Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>9</volume><issue>2</issue><spage>1048</spage><epage>1057</epage><pages>1048-1057</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>Asiatic acid, a triterpenoid derived from Centella asiatica, has been found to exhibit multiple bioactivities. In this study, we investigated the effects of asiatic acid on lipopolysaccharide (LPS)-induced neuroinflammation and explored the mechanism of its action in BV2 microglia. We found that asiatic acid (0.1 to 100 μM) treatment significantly attenuated nitric oxide (NO) production and inhibited inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner following LPS exposure. Asiatic acid reduced LPS-induced expression and secretion of inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in BV2 cells. In addition, asiatic acid enhanced Sirt1 expression, reduced NF-κB p65 acetylation, and suppressed NF-κB activation after LPS stimulation. However, EX-527, an inhibitor of Sirt1, abolished the inhibitory effects of asiatic acid on LPS-stimulated microglia activation. 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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | Acetylation Acids Activation Cytokines Inflammation Interleukin 6 Lipopolysaccharides Microglia NF-κB protein Nitric oxide Nitric-oxide synthase Signal transduction Signaling SIRT1 protein Tumor necrosis factor-TNF Tumor necrosis factor-α |
| title | Asiatic acid suppresses neuroinflammation in BV2 microglia via modulation of the Sirt1/NF-κB signaling pathway |
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