The efficient strategy of plasmid rescue from Tn5 mutants derived from Bradyrhizobium japonicum Is-1, based on whole genome sequence information of strain USDA110

We previously reported that Tn5 mutants derived from Bradyrhizobium japonicum Is-1 were isolated. In this report, Tn5-encoded kanamycin resistance cassette and its flanking sequence were concurrently cloned into cosmid vector from Tn5 mutants. In general, the genomic southern blot analysis is often...

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Veröffentlicht in:	Journal of the Faculty of Agriculture, Kyushu University Kyushu University, 2008-02, Vol.53 (1), p.27-31
Hauptverfasser:	Tsurumaru, H.(Kyushu Univ., Fukuoka (Japan)), Yamakawa, T, Tanaka, M, Sakai, M
Format:	Artikel
Sprache:	eng
Schlagworte:	BRADYRHIZOBIUM JAPONICUM ENZIMA DE RESTRICCION ENZYME DE RESTRICTION GENOMAS GENOME GENOMES MUTANT MUTANTES MUTANTS PLASMIDE PLASMIDIOS PLASMIDS RESTRICTION ENZYMES
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